(Linear) (Six-base) MAPSORT of: KIEE9434.seq Check: 8247 from: 1 to: 1011 KIEE9434.orf With 182 enzymes: SgfI * December 27, 2011 16:40 .. AflIII A'CryG_T Cuts at: 0 386 1011 Size: 386 625 AleI CACnn'nnGTG Cuts at: 0 665 1011 Size: 665 346 ApaLI G'TGCA_C Cuts at: 0 162 465 1011 Size: 162 303 546 Fragments arranged by size: 546 303 162 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 883 916 1011 Size: 883 33 95 Fragments arranged by size: 883 95 33 BanI G'GyrC_C Cuts at: 0 117 520 804 1011 Size: 117 403 284 207 Fragments arranged by size: 403 284 207 117 BanII G_rGCy'C Cuts at: 0 794 1011 Size: 794 217 BbeI G_GCGC'C Cuts at: 0 121 524 1011 Size: 121 403 487 Fragments arranged by size: 487 403 121 BbsI GAAGACnn'nnnn_ Cuts at: 0 318 504 1011 Size: 318 186 507 Fragments arranged by size: 507 318 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 597 631 1011 Size: 597 34 380 Fragments arranged by size: 597 380 34 BclI T'GATC_A Cuts at: 0 623 773 1011 Size: 623 150 238 Fragments arranged by size: 623 238 150 BglI GCCn_nnn'nGGC Cuts at: 0 971 1011 Size: 971 40 BlpI GC'TnA_GC Cuts at: 0 283 298 334 1011 Size: 283 15 36 677 Fragments arranged by size: 677 283 36 15 Bme1580I G_kGCm'C Cuts at: 0 43 166 469 809 1011 Size: 43 123 303 340 202 Fragments arranged by size: 340 303 202 123 43 BmgBI CAC'GTC Cuts at: 0 621 1011 Size: 621 390 BmrI ACTGGGnnnn_n' Cuts at: 0 659 831 1011 Size: 659 172 180 Fragments arranged by size: 659 180 172 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 813 1011 Size: 813 198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 1011 Size: 706 305 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 562 571 1011 Size: 562 9 440 Fragments arranged by size: 562 440 9 BsaAI yAC'GTr Cuts at: 0 146 389 572 1011 Size: 146 243 183 439 Fragments arranged by size: 439 243 183 146 BsaHI Gr'CG_yC Cuts at: 0 50 118 197 521 1011 Size: 50 68 79 324 490 Fragments arranged by size: 490 324 79 68 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 931 961 1011 Size: 931 30 50 Fragments arranged by size: 931 50 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 703 948 1011 Size: 703 245 63 Fragments arranged by size: 703 245 63 BseYI C'CCAG_C Cuts at: 0 311 411 1011 Size: 311 100 600 Fragments arranged by size: 600 311 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 379 495 552 643 922 1011 Size: 76 303 116 57 91 279 89 Fragments arranged by size: 303 279 116 91 89 76 57 BsiEI CG_ry'CG Cuts at: 0 228 309 1011 Size: 228 81 702 Fragments arranged by size: 702 228 81 BsiHKAI G_wGCw'C Cuts at: 0 166 469 563 587 794 1011 Size: 166 303 94 24 207 217 Fragments arranged by size: 303 217 207 166 94 24 BsmBI CGTCTCn'nnnn_ Cuts at: 0 57 1011 Size: 57 954 Bsp1286I G_dGCh'C Cuts at: 0 43 166 469 563 587 794 809 1011 Size: 43 123 303 94 24 207 15 202 Fragments arranged by size: 303 207 202 123 94 43 24 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 164 665 1011 Size: 164 501 346 Fragments arranged by size: 501 346 164 BsrBI CCG'CTC Cuts at: 0 183 1011 Size: 183 828 BsrDI GCAATG_nn' Cuts at: 0 603 1011 Size: 603 408 BsrFI r'CCGG_y Cuts at: 0 120 193 216 479 999 1011 Size: 120 73 23 263 520 12 Fragments arranged by size: 520 263 120 73 23 12 BssSI C'ACGA_G Cuts at: 0 6 462 640 1011 Size: 6 456 178 371 Fragments arranged by size: 456 371 178 6 BstAPI GCAn_nnn'nTGC Cuts at: 0 473 911 1011 Size: 473 438 100 Fragments arranged by size: 473 438 100 BstXI CCAn_nnnn'nTGG Cuts at: 0 72 786 843 961 1011 Size: 72 714 57 118 50 Fragments arranged by size: 714 118 72 57 50 BtgI C'CryG_G Cuts at: 0 167 320 800 1011 Size: 167 153 480 211 Fragments arranged by size: 480 211 167 153 DraIII CAC_nnn'GTG Cuts at: 0 473 1011 Size: 473 538 EaeI y'GGCC_r Cuts at: 0 191 290 317 484 1011 Size: 191 99 27 167 527 Fragments arranged by size: 527 191 167 99 27 EarI CTCTTCn'nnn_ Cuts at: 0 133 799 1011 Size: 133 666 212 Fragments arranged by size: 666 212 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 97 1011 Size: 97 914 EcoICRI GAG'CTC Cuts at: 0 792 1011 Size: 792 219 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 1011 Size: 706 305 EcoO109I rG'GnC_Cy Cuts at: 0 743 1011 Size: 743 268 HaeII r_GCGC'y Cuts at: 0 121 127 373 498 524 1011 Size: 121 6 246 125 26 487 Fragments arranged by size: 487 246 125 121 26 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 935 967 1011 Size: 935 32 44 Fragments arranged by size: 935 44 32 HincII GTy'rAC Cuts at: 0 174 1011 Size: 174 837 KasI G'GCGC_C Cuts at: 0 117 520 1011 Size: 117 403 491 Fragments arranged by size: 491 403 117 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 89 890 1011 Size: 89 801 121 Fragments arranged by size: 801 121 89 MslI CAynn'nnrTG Cuts at: 0 619 665 1011 Size: 619 46 346 Fragments arranged by size: 619 346 46 MspA1I CmG'CkG Cuts at: 0 210 311 333 453 1011 Size: 210 101 22 120 558 Fragments arranged by size: 558 210 120 101 22 NaeI GCC'GGC Cuts at: 0 122 195 218 481 1011 Size: 122 73 23 263 530 Fragments arranged by size: 530 263 122 73 23 NarI GG'CG_CC Cuts at: 0 118 521 1011 Size: 118 403 490 Fragments arranged by size: 490 403 118 NgoMIV G'CCGG_C Cuts at: 0 120 193 216 479 1011 Size: 120 73 23 263 532 Fragments arranged by size: 532 263 120 73 23 NspI r_CATG'y Cuts at: 0 180 1011 Size: 180 831 PmlI CAC'GTG Cuts at: 0 146 389 1011 Size: 146 243 622 Fragments arranged by size: 622 243 146 PpuMI rG'GwC_Cy Cuts at: 0 743 1011 Size: 743 268 PvuII CAG'CTG Cuts at: 0 453 1011 Size: 453 558 SacI G_AGCT'C Cuts at: 0 794 1011 Size: 794 217 SapI GCTCTTCn'nnn_ Cuts at: 0 133 799 1011 Size: 133 666 212 Fragments arranged by size: 666 212 133 SfoI GGC'GCC Cuts at: 0 119 522 1011 Size: 119 403 489 Fragments arranged by size: 489 403 119 SgrAI Cr'CCGG_yG Cuts at: 0 120 1011 Size: 120 891 SmlI C'TyrA_G Cuts at: 0 577 586 1011 Size: 577 9 425 Fragments arranged by size: 577 425 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 303 1011 Size: 303 708 TatI w'GTAC_w Cuts at: 0 878 1011 Size: 878 133 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 931 963 1011 Size: 931 32 48 Fragments arranged by size: 931 48 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 285 1011 Size: 285 726 Enzymes that do cut: AflIII AleI ApaLI BaeI BanI BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PmlI PpuMI PvuII SacI SapI SfoI SgrAI SmlI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI AvaI AvrII BamHI BbvCI BciVI BfrBI BglII BmtI Bpu10I BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI