################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbrPI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BseRI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HincII HindII HindIII HpaI HphI KpnI MfeI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 169 GACGTC GACGT^C AccI 487 GTCTAC GT^[AC][GT]AC AfaI 255 GTAC GT^AC AflIII 182 ACGCGT A^C[GA][CT]GT AseI 283 ATTAAT AT^TAAT AsnI 283 ATTAAT AT^TAAT AspHI 1035 GAGCAC G[AT]GC[AT]^C BalI 570 TGGCCA TGG^CCA BanI 213 GGCGCC G^G[CT][GA]CC BbeI 213 GGCGCC GGCGC^C BbsI 3 GAAGAC GAAGAC(2/6) BbvCI 760 GCTGAGG CCTCAGC(-5/-2) BclI 828 TGATCA T^GATCA BcnI 194 CCGGG CC^[GC]GG BglII 363 AGATCT A^GATCT BmyI 1035 GAGCAC G[AGT]GC[ACT]^C BpmI 945 CTGGAG CTGGAG(16/14) BpuAI 3 GAAGAC GAAGAC(2/6) BsaBI 358 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 978 GTGCAG GTGCAG(16/14) BsiEI 208 CGACCG CG[GA][CT]^CG BsiHKAI 1035 GAGCAC G[AT]GC[AT]^C BsmFI 304 GTCCC GGGAC(10/14) Bsp1286I 1035 GAGCAC G[AGT]GC[ACT]^C BspMI 346 ACCTGC ACCTGC(4/8) BsrDI 962 GCAATG GCAATG(2/0) BstXI 217 CCATCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 363 AGATCT [GA]^GATC[CT] EaeI 570 TGGCCA [CT]^GGCC[GA] EarI 1023 GAAGAG CTCTTC(1/4) FauI 79 CCCGC CCCGC(4/6) FbaI 828 TGATCA T^GATCA HaeII 213 GGCGCC [GA]GCGC^[CT] KasI 213 GGCGCC G^GCGCC Ksp632I 1023 GAAGAG CTCTTC(1/4) MaeII 170 ACGT A^CGT MaeIII 715 GTGAC ^GT[ACGT]AC MamI 358 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 363 AGATCT [GA]^GATC[CT] MluI 182 ACGCGT A^CGCGT MluNI 570 TGGCCA TGG^CCA MscI 570 TGGCCA TGG^CCA MslI 218 CATCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 213 GGCGCC GG^CGCC NciI 194 CCGGG CC^[GC]GG NspI 688 GCATGT [GA]CATG^[CT] PleI 681 GAGTC GAGTC(4/5) PshBI 283 ATTAAT AT^TAAT PvuII 493 CAGCTG CAG^CTG RsaI 255 GTAC GT^AC SfaNI 612 GATGC GCATC(5/9) SfoI 213 GGCGCC GGC^GCC SmlI 547 CTTGAG C^T[CT][GA]AG SspI 506 AATATT AAT^ATT StuI 54 AGGCCT AGG^CCT TfiI 1016 GAATC G^A[AT]TC Tsp45I 715 GTGAC ^GT[GC]AC XbaI 427 TCTAGA T^CTAGA XhoII 363 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 169 GACGTC G[GA]^CG[CT]C 213 GGCGCC AlwI 357 GGATC GGATC(4/5) 950 GGATC AlwNI 241 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 583 CAGGCACTG BsaHI 169 GACGTC G[GA]^CG[CT]C 213 GGCGCC BsaI 550 GAGACC GGTCTC(1/5) 598 GAGACC BsiYI 75 CCTCCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 79 CCCGCGGCAGG BslI 75 CCTCCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 79 CCCGCGGCAGG BsmAI 550 GAGAC GTCTC(1/5) 598 GAGAC BsrI 393 CCAGT ACTGG(1/-1) 748 CCAGT BssHII 103 GCGCGC G^CGCGC 105 GCGCGC BstNI 258 CCTGG CC^[AT]GG 708 CCTGG DsaI 43 CCGCGG C^C[GA][CT]GG 80 CCGCGG EcoRII 258 CCTGG ^CC[AT]GG 708 CCTGG HgaI 181 GACGC GACGC(5/10) 62 GCGTC Hin1I 169 GACGTC G[GA]^CG[CT]C 213 GGCGCC HinfI 681 GAGTC G^A[ACGT]TC 1016 GAATC KspI 43 CCGCGG CCGC^GG 80 CCGCGG MvaI 258 CCTGG CC^[AT]GG 708 CCTGG NlaIV 25 GGAACC GG[ACGT]^[ACGT]CC 213 GGCGCC PstI 124 CTGCAG CTGCA^G 377 CTGCAG SacII 43 CCGCGG CCGC^GG 80 CCGCGG SfcI 124 CTGCAG C^T[GA][CT]AG 377 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AluI BbvI BcgI BfaI BsaJI BsrFI BssKI BstF5I BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeI MboI MboII MnlI MseI MspA1I MspI MvnI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI TaqI Tru9I TseI Tsp509I TspRI TthHB8I