(Circular) (Six-base) MAPSORT of: pFN21AE3137.seq Check: 1171 from: 1 to: 5749 pFN21AE3137 5749 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2178 3559 278 Size: 53 83 186 936 642 1381 2468 Fragments arranged by size: 2468 1381 936 642 186 83 53 AccI GT'mk_AC Cuts at: 1937 2493 3092 1937 Size: 556 599 4594 Fragments arranged by size: 4594 599 556 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2346 3812 5196 5464 1632 Size: 714 1466 1384 268 1917 Fragments arranged by size: 1917 1466 1384 714 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 2187 4648 5625 1203 Size: 984 2461 977 1327 Fragments arranged by size: 2461 1327 984 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2251 2593 3492 5064 2251 Size: 342 899 1572 2936 Fragments arranged by size: 2936 1572 899 342 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 2289 4303 160 Size: 872 1257 2014 1606 Fragments arranged by size: 2014 1606 1257 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 3074 1870 Size: 73 1131 4545 Fragments arranged by size: 4545 1131 73 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 3070 4525 618 Size: 325 217 627 431 852 1455 1842 Fragments arranged by size: 1842 1455 852 627 431 325 217 BanII G_rGCy'C Cuts at: 1391 3068 1391 Size: 1677 4072 BbeI G_GCGC'C Cuts at: 2222 2222 Size: 5749 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 928 Size: 971 116 4662 Fragments arranged by size: 4662 971 116 BbvCI CC'TCA_GC Cuts at: 2766 2766 Size: 5749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2046 2079 2080 2113 2682 2716 3940 Size: 34 109 33 1 33 569 34 1224 Cuts at: 3940 3974 1903 Size: 34 3678 Fragments arranged by size: 3678 1224 569 109 34 34 34 33 33 1 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 2833 1548 Size: 1285 4464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4360 136 Size: 107 122 71 631 605 2688 1525 Fragments arranged by size: 2688 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2368 5744 2368 Size: 3376 2373 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 3810 4966 1790 Size: 2020 1156 2573 Fragments arranged by size: 2573 2020 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2392 3480 4438 Size: 416 53 83 399 24 975 1088 958 Cuts at: 4438 442 Size: 1753 Fragments arranged by size: 1753 1088 975 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2971 4409 1239 Size: 1732 1438 2579 Fragments arranged by size: 2579 1732 1438 Bpu10I CC'TnA_GC Cuts at: 2766 2766 Size: 5749 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2573 3874 4739 5037 2573 Size: 1301 865 298 3285 Fragments arranged by size: 3285 1301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2549 2597 4412 882 Size: 617 1050 48 1815 2219 Fragments arranged by size: 2219 1815 1050 617 48 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 2367 5743 2367 Size: 3376 2373 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 2219 3556 3938 Size: 53 83 186 936 642 44 1337 382 Cuts at: 3938 4573 275 Size: 635 1451 Fragments arranged by size: 1451 1337 936 642 635 382 186 83 53 44 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5524 Fragments arranged by size: 5524 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5749 BseYI C'CCAG_C Cuts at: 4952 5448 4952 Size: 496 5253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3004 1163 Size: 573 1268 3908 Fragments arranged by size: 3908 1268 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2216 3199 3960 4988 1540 Size: 275 186 215 983 761 1028 2301 Fragments arranged by size: 2301 1028 983 761 275 215 186 BsiHKAI G_wGCw'C Cuts at: 3044 3810 3895 4966 3044 Size: 766 85 1071 3827 Fragments arranged by size: 3827 1071 766 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3044 3068 3810 3895 4966 1391 Size: 399 1254 24 742 85 1071 2174 Fragments arranged by size: 2174 1254 1071 742 399 85 24 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2360 3104 844 Size: 1516 744 3489 Fragments arranged by size: 3489 1516 744 BsrBI CCG'CTC Cuts at: 1394 1823 3636 1394 Size: 429 1813 3507 Fragments arranged by size: 3507 1813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2974 4244 4418 65 Size: 1183 1726 1270 174 1396 Fragments arranged by size: 1726 1396 1270 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 2124 2215 4393 4580 1700 Size: 110 223 91 91 2178 187 2869 Fragments arranged by size: 2869 2178 223 187 110 91 91 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 2108 2110 1925 Size: 183 2 5564 Fragments arranged by size: 5564 183 2 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2229 2229 Size: 5749 BstYI r'GATC_y Cuts at: 1972 2368 3079 3446 3831 3848 5300 5386 5398 Size: 396 711 367 385 17 1452 86 12 Cuts at: 5398 5744 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 711 396 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2048 2085 513 Size: 552 983 37 4177 Fragments arranged by size: 4177 983 552 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 2591 2736 3017 3265 4059 4079 2591 Size: 145 281 248 794 20 4261 Fragments arranged by size: 4261 794 281 248 145 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2575 3196 4085 Size: 54 1425 50 275 18 745 621 889 Cuts at: 4085 5714 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 745 621 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3023 3679 1353 Size: 1670 656 3423 Fragments arranged by size: 3423 1670 656 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2346 2971 3812 4409 5196 5464 1239 Size: 393 714 625 841 597 787 268 1524 Fragments arranged by size: 1524 841 787 714 625 597 393 268 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2222 4896 1678 Size: 544 2674 2531 Fragments arranged by size: 2674 2531 544 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 2475 2507 2842 Size: 32 135 32 812 32 59 32 335 Cuts at: 2842 2874 4390 4422 4464 4496 1373 Size: 32 1516 32 42 32 2626 Fragments arranged by size: 2626 1516 812 335 135 59 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3093 3339 669 Size: 988 281 1155 246 3079 Fragments arranged by size: 3079 1155 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KasI G'GCGC_C Cuts at: 2218 2218 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 2187 5625 2187 Size: 3438 2311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4863 5047 1305 Size: 3558 184 2007 Fragments arranged by size: 3558 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2577 10 Size: 54 1425 343 745 3182 Fragments arranged by size: 3182 1425 745 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2227 3707 4066 4225 518 Size: 648 1061 1480 359 159 2042 Fragments arranged by size: 2042 1480 1061 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2087 2500 3165 3842 4990 5235 1606 Size: 444 37 413 665 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 665 444 413 245 37 NarI GG'CG_CC Cuts at: 2219 2219 Size: 5749 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5197 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2697 3107 4652 1132 Size: 97 1468 410 1545 2229 Fragments arranged by size: 2229 1545 1468 410 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5749 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 2348 3319 2348 Size: 971 4778 PstI C_TGCA'G Cuts at: 830 2133 2386 3101 830 Size: 1303 253 715 3478 Fragments arranged by size: 3478 1303 715 253 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2500 2500 Size: 5749 SacII CC_GC'GG Cuts at: 2051 2088 2051 Size: 37 5712 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2129 2382 3097 4232 4913 5104 826 Size: 219 1084 253 715 1135 681 191 1471 Fragments arranged by size: 1471 1135 1084 715 681 253 219 191 SfoI GGC'GCC Cuts at: 2220 2220 Size: 5749 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 3853 4754 5016 820 Size: 197 926 609 1301 901 262 1553 Fragments arranged by size: 1553 1301 926 901 609 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 2513 3673 5 Size: 47 2461 1160 2081 Fragments arranged by size: 2461 2081 1160 47 StuI AGG'CCT Cuts at: 1517 2061 1517 Size: 544 5205 StyI C'CwwG_G Cuts at: 513 1065 3188 513 Size: 552 2123 3074 Fragments arranged by size: 3074 2123 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2283 2329 3776 3793 3946 4131 4576 Size: 1255 703 46 1447 17 153 185 445 Cuts at: 4576 325 Size: 1498 Fragments arranged by size: 1498 1447 1255 703 445 185 153 46 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3995 5707 96 Size: 274 80 33 51 1443 2018 1712 138 Fragments arranged by size: 2018 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5709 5741 5709 Size: 32 5717 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 2432 3085 2432 Size: 653 5096 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2176 3557 276 Size: 53 83 186 936 642 1381 2468 Fragments arranged by size: 2468 1381 936 642 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI