Restriction map for
pFN21AE3137
(Linear) (Six-base) MAPSORT of: KIEE3137.seq Check: 4671 from: 1 to: 1044
KIEE3137.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AatII G_ACGT'C
Cuts at: 0 173 1044
Size: 173 871
AccI GT'mk_AC
Cuts at: 0 488 1044
Size: 488 556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 1044
Size: 341 703
AflIII A'CryG_T
Cuts at: 0 182 1044
Size: 182 862
AlwNI CAG_nnn'CTG
Cuts at: 0 246 588 1044
Size: 246 342 456
Fragments arranged by size:
456 342 246
AseI AT'TA_AT
Cuts at: 0 284 1044
Size: 284 760
BanI G'GyrC_C
Cuts at: 0 213 1044
Size: 213 831
BbeI G_GCGC'C
Cuts at: 0 217 1044
Size: 217 827
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 1044
Size: 10 1034
BbvCI CC'TCA_GC
Cuts at: 0 761 1044
Size: 761 283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 41 74 75 108 677 711 1044
Size: 41 33 1 33 569 34 333
Fragments arranged by size:
569 333 41 34 33 33 1
BclI T'GATC_A
Cuts at: 0 828 1044
Size: 828 216
BglII A'GATC_T
Cuts at: 0 363 1044
Size: 363 681
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 1044
Size: 387 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 966 1044
Size: 966 78
Bpu10I CC'TnA_GC
Cuts at: 0 761 1044
Size: 761 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1044
Size: 568 476
BsaI GGTCTCn'nnnn_
Cuts at: 0 544 592 1044
Size: 544 48 452
Fragments arranged by size:
544 452 48
BsaBI GATnn'nnATC
Cuts at: 0 362 1044
Size: 362 682
BsaHI Gr'CG_yC
Cuts at: 0 170 214 1044
Size: 170 44 830
Fragments arranged by size:
830 170 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 999 1044
Size: 999 45
BsiEI CG_ry'CG
Cuts at: 0 211 1044
Size: 211 833
BsiHKAI G_wGCw'C
Cuts at: 0 1039 1044
Size: 1039 5
Bsp1286I G_dGCh'C
Cuts at: 0 1039 1044
Size: 1039 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 355 1044
Size: 355 689
BsrDI GCAATG_nn'
Cuts at: 0 969 1044
Size: 969 75
BsrFI r'CCGG_y
Cuts at: 0 28 119 210 1044
Size: 28 91 91 834
Fragments arranged by size:
834 91 91 28
BssHII G'CGCG_C
Cuts at: 0 103 105 1044
Size: 103 2 939
Fragments arranged by size:
939 103 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 224 1044
Size: 224 820
BstYI r'GATC_y
Cuts at: 0 363 1044
Size: 363 681
BtgI C'CryG_G
Cuts at: 0 43 80 1044
Size: 43 37 964
Fragments arranged by size:
964 43 37
BtsI GCAGTG_nn'
Cuts at: 0 586 731 1012 1044
Size: 586 145 281 32
Fragments arranged by size:
586 281 145 32
EaeI y'GGCC_r
Cuts at: 0 570 1044
Size: 570 474
EarI CTCTTCn'nnn_
Cuts at: 0 1018 1044
Size: 1018 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 966 1044
Size: 341 625 78
Fragments arranged by size:
625 341 78
HaeII r_GCGC'y
Cuts at: 0 217 1044
Size: 217 827
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 470 502 837 869 1044
Size: 379 32 59 32 335 32 175
Fragments arranged by size:
379 335 175 59 32 32 32
KasI G'GCGC_C
Cuts at: 0 213 1044
Size: 213 831
MluI A'CGCG_T
Cuts at: 0 182 1044
Size: 182 862
MscI TGG'CCA
Cuts at: 0 572 1044
Size: 572 472
MslI CAynn'nnrTG
Cuts at: 0 222 1044
Size: 222 822
MspA1I CmG'CkG
Cuts at: 0 45 82 495 1044
Size: 45 37 413 549
Fragments arranged by size:
549 413 45 37
NarI GG'CG_CC
Cuts at: 0 214 1044
Size: 214 830
NspI r_CATG'y
Cuts at: 0 692 1044
Size: 692 352
PsiI TTA'TAA
Cuts at: 0 343 1044
Size: 343 701
PstI C_TGCA'G
Cuts at: 0 128 381 1044
Size: 128 253 663
Fragments arranged by size:
663 253 128
PvuII CAG'CTG
Cuts at: 0 495 1044
Size: 495 549
SacII CC_GC'GG
Cuts at: 0 46 83 1044
Size: 46 37 961
Fragments arranged by size:
961 46 37
SfcI C'TryA_G
Cuts at: 0 124 377 1044
Size: 124 253 667
Fragments arranged by size:
667 253 124
SfoI GGC'GCC
Cuts at: 0 215 1044
Size: 215 829
SmlI C'TyrA_G
Cuts at: 0 547 1044
Size: 547 497
SspI AAT'ATT
Cuts at: 0 508 1044
Size: 508 536
StuI AGG'CCT
Cuts at: 0 56 1044
Size: 56 988
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 324 1044
Size: 278 46 720
Fragments arranged by size:
720 278 46
XbaI T'CTAG_A
Cuts at: 0 427 1044
Size: 427 617
ZraI GAC'GTC
Cuts at: 0 171 1044
Size: 171 873
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI AseI BanI BbeI
BbsI BbvCI BcgI BclI BglII BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BssHII BstXI BstYI BtgI BtsI
EaeI EarI Eco57MI HaeII Hin4I KasI MluI MscI
MslI MspA1I NarI NspI PsiI PstI PvuII SacII
SfcI SfoI SmlI SspI StuI TaqII XbaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BciVI BfrBI BglI BlpI
Bme1580I BmgBI BmtI BplI BsaAI BsaWI BsaXI BseRI
BseYI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StyI SwaI TatI TstI Tth111I
XcmI XhoI XmaI XmnI