################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccII AcyI AflII AgeI AlwNI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BanI BbeI BbvCI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI BstBI BstUI CelII ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 1771 GTATAC GT^[AC][GT]AC AccIII 121 TCCGGA T^CCGGA AclI 1980 AACGTT AA^CGTT AcsI 2012 GAATTC [GA]^AATT[CT] AflIII 458 ACACGT A^C[GA][CT]GT AhdI 1540 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 979 GTGCAC G^TGCAC AocI 1516 CCTGAGG CC^T[ACGT]AGG ApaI 637 GGGCCC GGGCC^C ApaLI 979 GTGCAC G^TGCAC ApoI 2012 GAATTC [GA]^AATT[CT] AspEI 1540 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1565 TGGCCA TGG^CCA BbrPI 610 CACGTG CAC^GTG BglI 639 GCCCGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1820 AGATCT A^GATCT BsaWI 121 TCCGGA [AT]^CCGG[AT] BseAI 121 TCCGGA T^CCGGA BsmBI 548 GAGACG CGTCTC(1/5) Bsp1407I 1931 TGTACA T^GTACA BspEI 121 TCCGGA T^CCGGA BsrGI 1931 TGTACA T^GTACA Bst1107I 1771 GTATAC GTA^TAC BstAPI 1956 GCATGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1820 AGATCT [GA]^GATC[CT] BstZ17I 1771 GTATAC GTA^TAC Bsu36I 1516 CCTGAGG CC^T[ACGT]AGG CfoI 820 GCGC GCG^C DraII 1145 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 1540 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1516 CCTGAGG CC^T[ACGT]AGG EcoO109I 1145 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 2012 GAATTC G^AATTC EcoRV 1684 GATATC GAT^ATC FauI 1062 CCCGC CCCGC(4/6) HaeII 819 AGCGCC [GA]GCGC^[CT] HhaI 820 GCGC GCG^C HinP1I 820 GCGC G^CGC MflI 1820 AGATCT [GA]^GATC[CT] MluNI 1565 TGGCCA TGG^CCA MroI 121 TCCGGA T^CCGGA MscI 1565 TGGCCA TGG^CCA NcoI 41 CCATGG C^CATGG NspI 1956 GCATGC [GA]CATG^[CT] PflMI 296 CCATGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 610 CACGTG CAC^GTG PmlI 610 CACGTG CAC^GTG PpuMI 1145 AGGACCC [GA]G^G[AT]CC[CT] Psp1406I 1980 AACGTT AA^CGTT SmaI 1480 CCCGGG CCC^GGG SphI 1956 GCATGC GCATG^C SspBI 1931 TGTACA T^GTACA TfiI 1854 GATTC G^A[AT]TC Van91I 296 CCATGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1820 AGATCT [GA]^GATC[CT] XmaCI 1480 CCCGGG C^CCGGG XmaI 1480 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwI 602 GGATC GGATC(4/5) 2007 GGATC AspHI 188 GAGCAC G[AT]GC[AT]^C 979 GTGCAC AvaI 675 CTCGGG C^[CT]CG[GA]G 1480 CCCGGG BbsI 1999 GAAGAC GAAGAC(2/6) 889 GTCTTC BcgI 726 CGAGGCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 330 GCAGGAGCATCG BciVI 87 GGATAC GTATCC(6/5) 998 GTATCC BpuAI 1999 GAAGAC GAAGAC(2/6) 889 GTCTTC BsaAI 459 CACGTA [CT]AC^GT[GA] 610 CACGTG BsgI 1395 CTGCAC GTGCAG(16/14) 1917 CTGCAC BsiHKAI 188 GAGCAC G[AT]GC[AT]^C 979 GTGCAC BsoBI 675 CTCGGG C^[CT]CG[GA]G 1480 CCCGGG BstEII 36 GGTCACC G^GT[ACGT]ACC 1269 GGTCACC BstPI 36 GGTCACC G^GT[ACGT]ACC 1269 GGTCACC BstXI 1568 CCAGAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1732 CCAGTCAAGTGG DdeI 1031 CTGAG C^T[ACGT]AG 1517 CTGAG EarI 1089 CTCTTC CTCTTC(1/4) 827 GAAGAG EcoO65I 36 GGTCACC G^GT[ACGT]ACC 1269 GGTCACC EcoT14I 41 CCATGG C^C[AT][AT]GG 1259 CCAAGG Ksp632I 1089 CTCTTC CTCTTC(1/4) 827 GAAGAG NaeI 519 GCCGGC GCC^GGC 972 GCCGGC NgoMIV 519 GCCGGC G^CCGGC 972 GCCGGC PleI 1673 GACTC GAGTC(4/5) 1033 GAGTC PvuII 6 CAGCTG CAG^CTG 1561 CAGCTG ScaI 1490 AGTACT AGT^ACT 1580 AGTACT SmlI 658 CTCAAG C^T[CT][GA]AG 667 CTCAAG StyI 41 CCATGG C^C[AT][AT]GG 1259 CCAAGG TaqI 1608 TCGA T^CGA 2010 TCGA TthHB8I 1608 TCGA T^CGA 2010 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AluI AvaII BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DpnII DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI XcmI