(Linear) (Six-base) MAPSORT of: KIEE2539.seq Check: 2502 from: 1 to: 2040 KIEE2539.orf With 182 enzymes: SgfI * September 19, 2014 17:03 .. AccI GT'mk_AC Cuts at: 0 1772 2040 Size: 1772 268 AclI AA'CG_TT Cuts at: 0 1981 2040 Size: 1981 59 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 945 1903 2040 Size: 57 888 958 137 Fragments arranged by size: 958 888 137 57 AflIII A'CryG_T Cuts at: 0 458 2040 Size: 458 1582 AhdI GACnn_n'nnGTC Cuts at: 0 1545 2040 Size: 1545 495 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 672 704 2040 Size: 672 32 1336 Fragments arranged by size: 1336 672 32 ApaI G_GGCC'C Cuts at: 0 641 2040 Size: 641 1399 ApaLI G'TGCA_C Cuts at: 0 979 2040 Size: 979 1061 ApoI r'AATT_y Cuts at: 0 2012 2040 Size: 2012 28 AvaI C'yCGr_G Cuts at: 0 675 1480 2040 Size: 675 805 560 Fragments arranged by size: 805 675 560 BanII G_rGCy'C Cuts at: 0 641 1470 1840 2040 Size: 641 829 370 200 Fragments arranged by size: 829 641 370 200 BbsI GAAGACnn'nnnn_ Cuts at: 0 882 2006 2040 Size: 882 1124 34 Fragments arranged by size: 1124 882 34 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 319 353 715 749 2040 Size: 319 34 362 34 1291 Fragments arranged by size: 1291 362 319 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 81 1009 2040 Size: 81 928 1031 Fragments arranged by size: 1031 928 81 BglI GCCn_nnn'nGGC Cuts at: 0 645 2040 Size: 645 1395 BglII A'GATC_T Cuts at: 0 1820 2040 Size: 1820 220 Bme1580I G_kGCm'C Cuts at: 0 641 983 1967 2040 Size: 641 342 984 73 Fragments arranged by size: 984 641 342 73 BmrI ACTGGGnnnn_n' Cuts at: 0 1192 2040 Size: 1192 848 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1056 1452 1477 2040 Size: 1056 396 25 563 Fragments arranged by size: 1056 563 396 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 393 883 1733 2040 Size: 393 490 850 307 Fragments arranged by size: 850 490 393 307 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 643 652 2040 Size: 643 9 1388 Fragments arranged by size: 1388 643 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 762 1128 1968 2040 Size: 762 366 840 72 Fragments arranged by size: 840 762 366 72 BsaAI yAC'GTr Cuts at: 0 461 612 2040 Size: 461 151 1428 Fragments arranged by size: 1428 461 151 BsaWI w'CCGG_w Cuts at: 0 121 2040 Size: 121 1919 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 536 566 2040 Size: 536 30 1474 Fragments arranged by size: 1474 536 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 560 1032 1534 2040 Size: 560 472 502 506 Fragments arranged by size: 560 506 502 472 BseYI C'CCAG_C Cuts at: 0 8 270 584 736 2040 Size: 8 262 314 152 1304 Fragments arranged by size: 1304 314 262 152 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1380 1902 2040 Size: 1380 522 138 Fragments arranged by size: 1380 522 138 BsiHKAI G_wGCw'C Cuts at: 0 192 983 2040 Size: 192 791 1057 Fragments arranged by size: 1057 791 192 BsmBI CGTCTCn'nnnn_ Cuts at: 0 542 2040 Size: 542 1498 Bsp1286I G_dGCh'C Cuts at: 0 192 641 983 1470 1840 1967 2040 Size: 192 449 342 487 370 127 73 Fragments arranged by size: 487 449 370 342 192 127 73 BspEI T'CCGG_A Cuts at: 0 121 2040 Size: 121 1919 BsrFI r'CCGG_y Cuts at: 0 494 519 972 2040 Size: 494 25 453 1068 Fragments arranged by size: 1068 494 453 25 BsrGI T'GTAC_A Cuts at: 0 1931 2040 Size: 1931 109 BstAPI GCAn_nnn'nTGC Cuts at: 0 1962 2040 Size: 1962 78 BstEII G'GTnAC_C Cuts at: 0 36 1269 2040 Size: 36 1233 771 Fragments arranged by size: 1233 771 36 BstXI CCAn_nnnn'nTGG Cuts at: 0 1575 1739 2040 Size: 1575 164 301 Fragments arranged by size: 1575 301 164 BstYI r'GATC_y Cuts at: 0 1820 2040 Size: 1820 220 BstZ17I GTA'TAC Cuts at: 0 1773 2040 Size: 1773 267 Bsu36I CC'TnA_GG Cuts at: 0 1517 2040 Size: 1517 523 BtgI C'CryG_G Cuts at: 0 41 1274 1859 2040 Size: 41 1233 585 181 Fragments arranged by size: 1233 585 181 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 322 2040 Size: 322 1718 BtsI GCAGTG_nn' Cuts at: 0 1241 1967 2040 Size: 1241 726 73 Fragments arranged by size: 1241 726 73 EaeI y'GGCC_r Cuts at: 0 245 1277 1565 2040 Size: 245 1032 288 475 Fragments arranged by size: 1032 475 288 245 EarI CTCTTCn'nnn_ Cuts at: 0 822 1095 2040 Size: 822 273 945 Fragments arranged by size: 945 822 273 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 745 1243 2040 Size: 745 498 797 Fragments arranged by size: 797 745 498 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 393 883 945 1733 1903 2040 Size: 57 336 490 62 788 170 137 Fragments arranged by size: 788 490 336 170 137 62 57 EcoO109I rG'GnC_Cy Cuts at: 0 1146 2040 Size: 1146 894 EcoRI G'AATT_C Cuts at: 0 2012 2040 Size: 2012 28 EcoRV GAT'ATC Cuts at: 0 1686 2040 Size: 1686 354 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 975 2040 Size: 975 1065 HaeII r_GCGC'y Cuts at: 0 823 2040 Size: 823 1217 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 100 132 1315 1347 1531 1563 1780 1812 Size: 100 32 1183 32 184 32 217 32 Cuts at: 1812 2040 Size: 228 Fragments arranged by size: 1183 228 217 184 100 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 522 1079 2040 Size: 522 557 961 Fragments arranged by size: 961 557 522 MscI TGG'CCA Cuts at: 0 1567 2040 Size: 1567 473 MslI CAynn'nnrTG Cuts at: 0 470 1108 1784 1939 2040 Size: 470 638 676 155 101 Fragments arranged by size: 676 638 470 155 101 MspA1I CmG'CkG Cuts at: 0 8 1065 1563 2040 Size: 8 1057 498 477 Fragments arranged by size: 1057 498 477 8 NaeI GCC'GGC Cuts at: 0 521 974 2040 Size: 521 453 1066 Fragments arranged by size: 1066 521 453 NcoI C'CATG_G Cuts at: 0 41 2040 Size: 41 1999 NgoMIV G'CCGG_C Cuts at: 0 519 972 2040 Size: 519 453 1068 Fragments arranged by size: 1068 519 453 NspI r_CATG'y Cuts at: 0 1960 2040 Size: 1960 80 PasI CC'CwG_GG Cuts at: 0 1194 1849 2040 Size: 1194 655 191 Fragments arranged by size: 1194 655 191 PflMI CCAn_nnn'nTGG Cuts at: 0 302 2040 Size: 302 1738 PmlI CAC'GTG Cuts at: 0 612 2040 Size: 612 1428 PpuMI rG'GwC_Cy Cuts at: 0 1146 2040 Size: 1146 894 PspOMI G'GGCC_C Cuts at: 0 637 2040 Size: 637 1403 PvuII CAG'CTG Cuts at: 0 8 1563 2040 Size: 8 1555 477 Fragments arranged by size: 1555 477 8 ScaI AGT'ACT Cuts at: 0 1492 1582 2040 Size: 1492 90 458 Fragments arranged by size: 1492 458 90 SmaI CCC'GGG Cuts at: 0 1482 2040 Size: 1482 558 SmlI C'TyrA_G Cuts at: 0 658 667 2040 Size: 658 9 1373 Fragments arranged by size: 1373 658 9 SphI G_CATG'C Cuts at: 0 1960 2040 Size: 1960 80 StyI C'CwwG_G Cuts at: 0 41 1259 2040 Size: 41 1218 781 Fragments arranged by size: 1218 781 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 739 2040 Size: 739 1301 TatI w'GTAC_w Cuts at: 0 1490 1580 1931 2040 Size: 1490 90 351 109 Fragments arranged by size: 1490 351 109 90 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 299 1062 1629 2040 Size: 299 763 567 411 Fragments arranged by size: 763 567 411 299 XmaI C'CCGG_G Cuts at: 0 1480 2040 Size: 1480 560 Enzymes that do cut: AccI AclI AcuI AflIII AhdI AloI ApaI ApaLI ApoI AvaI BanII BbsI BcgI BciVI BglI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BsrFI BsrGI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PmlI PpuMI PspOMI PvuII ScaI SmaI SmlI SphI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AleI AlfI AlwNI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BclI BfrBI BlpI BmgBI BmtI Bpu10I BsaBI BsaHI BsiEI BsiWI BsmI BspHI BspMI BsrBI BsrDI BssHII BssSI BstBI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI