Restriction map for
pFN21AE2011
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII AclI AcsI AflII AhdI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsgI BsiWI Bsp1407I
BspDI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI MamI MfeI MluI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1591 GGTACC G^GTACC
AccI 922 GTCTAC GT^[AC][GT]AC
AccIII 1626 TCCGGA T^CCGGA
AcyI 1462 GGCGTC G[GA]^CG[CT]C
AflIII 1574 ACGTGT A^C[GA][CT]GT
AgeI 537 ACCGGT A^CCGGT
Alw44I 434 GTGCAC G^TGCAC
AocI 456 CCTGAGG CC^T[ACGT]AGG
ApaLI 434 GTGCAC G^TGCAC
Asp718I 1591 GGTACC G^GTACC
AspHI 434 GTGCAC G[AT]GC[AT]^C
AspI 370 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1591 GGTACC G^G[CT][GA]CC
BfaI 574 CTAG C^TAG
BglI 1031 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1462 GGCGTC G[GA]^CG[CT]C
BseAI 1626 TCCGGA T^CCGGA
BsiHKAI 434 GTGCAC G[AT]GC[AT]^C
BsmBI 1207 GAGACG CGTCTC(1/5)
BsmFI 339 GTCCC GGGAC(10/14)
BsmI 1555 GAATGC GAATGC(1/-1)
BspEI 1626 TCCGGA T^CCGGA
BspHI 794 TCATGA T^CATGA
BsrBI 856 GAGCGG CCGCTC(-3/-3)
BsrFI 537 ACCGGT [GA]^CCGG[CT]
BssSI 448 CACGAG CACGAG(-5/-1)
Bsu36I 456 CCTGAGG CC^T[ACGT]AGG
Cfr10I 537 ACCGGT [GA]^CCGG[CT]
DrdI 1192 GACCGCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 456 CCTGAGG CC^T[ACGT]AGG
HgaI 1463 GCGTC GACGC(5/10)
Hin1I 1462 GGCGTC G[GA]^CG[CT]C
KpnI 1591 GGTACC GGTAC^C
MaeI 574 CTAG C^TAG
MroI 1626 TCCGGA T^CCGGA
NotI 1157 GCGGCCGC GC^GGCCGC
NspI 491 GCATGT [GA]CATG^[CT]
PaeR7I 712 CTCGAG C^TCGAG
PflFI 370 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 537 ACCGGT A^CCGGT
RcaI 794 TCATGA T^CATGA
SexAI 371 ACCTGGT A^CC[AT]GGT
SmlI 712 CTCGAG C^T[CT][GA]AG
StuI 220 AGGCCT AGG^CCT
Tth111I 370 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 218 CCAGGCCTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 712 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AlwNI 244 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1288 CAGAGGCTG
Aor51HI 644 AGCGCT AGC^GCT
1361 AGCGCT
AvaI 712 CTCGAG C^[CT]CG[GA]G
1009 CCCGAG
BanII 1092 GAGCCC G[GA]GC[CT]^C
1512 GGGCTC
BbsI 127 GAAGAC GAAGAC(2/6)
898 GAAGAC
BglII 930 AGATCT A^GATCT
1605 AGATCT
BpuAI 127 GAAGAC GAAGAC(2/6)
898 GAAGAC
BsiEI 1029 CGGCCG CG[GA][CT]^CG
1158 CGGCCG
BsoBI 712 CTCGAG C^[CT]CG[GA]G
1009 CCCGAG
BstEII 540 GGTGACC G^GT[ACGT]ACC
805 GGTGACC
BstPI 540 GGTGACC G^GT[ACGT]ACC
805 GGTGACC
EagI 1029 CGGCCG C^GGCCG
1158 CGGCCG
EarI 1660 CTCTTC CTCTTC(1/4)
657 GAAGAG
EclXI 1029 CGGCCG C^GGCCG
1158 CGGCCG
Eco47III 644 AGCGCT AGC^GCT
1361 AGCGCT
Eco52I 1029 CGGCCG C^GGCCG
1158 CGGCCG
EcoNI 711 CCTCGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1337 CCTTCTTCAGG
EcoO65I 540 GGTGACC G^GT[ACGT]ACC
805 GGTGACC
HaeII 644 AGCGCT [GA]GCGC^[CT]
1361 AGCGCT
HincII 721 GTCAAC GT[CT]^[GA]AC
739 GTCAAC
HindII 721 GTCAAC GT[CT]^[GA]AC
739 GTCAAC
Ksp632I 1660 CTCTTC CTCTTC(1/4)
657 GAAGAG
KspI 1026 CCGCGG CCGC^GG
1155 CCGCGG
MaeII 1427 ACGT A^CGT
1574 ACGT
MseI 1562 TTAA T^TAA
1718 TTAA
MspA1I 1026 CCGCGG C[AC]G^C[GT]G
1155 CCGCGG
NcoI 686 CCATGG C^CATGG
1528 CCATGG
PflMI 366 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1523 CCATCCCATGG
PpuMI 1064 AGGTCCT [GA]G^G[AT]CC[CT]
1199 GGGTCCT
PstI 159 CTGCAG CTGCA^G
1316 CTGCAG
SacII 1026 CCGCGG CCGC^GG
1155 CCGCGG
Tru9I 1562 TTAA T^TAA
1718 TTAA
Tsp509I 312 AATT ^AATT
1684 AATT
Van91I 366 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1523 CCATCCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BalI BbvI BcnI BmyI
BpmI BsaI BsaJI BsaWI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MluNI MnlI MscI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII