(Circular) (Six-base) MAPSORT of: pFN21AE2011.seq Check: 1690 from: 1 to: 6475 pFN21AE2011 6475 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2928 3818 1937 Size: 991 890 4594 Fragments arranged by size: 4594 991 890 Acc65I G'GTAC_C Cuts at: 3596 3796 3596 Size: 200 6275 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2126 2837 3331 4538 5922 6190 1632 Size: 494 711 494 1207 1384 268 1917 Fragments arranged by size: 1917 1384 1207 711 494 494 268 AfeI AGC'GCT Cuts at: 2651 3368 2651 Size: 717 5758 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 3579 5374 6351 1203 Size: 2376 1795 977 1327 Fragments arranged by size: 2376 1795 1327 977 AgeI A'CCGG_T Cuts at: 2542 5306 2542 Size: 2764 3711 AhdI GACnn_n'nnGTC Cuts at: 5204 5204 Size: 6475 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3287 1184 Size: 2103 4372 AlwNI CAG_nnn'CTG Cuts at: 2254 3298 4218 5790 2254 Size: 1044 920 1572 2939 Fragments arranged by size: 2939 1572 1044 920 ApaLI G'TGCA_C Cuts at: 2439 4532 5688 2439 Size: 2093 1156 3226 Fragments arranged by size: 3226 2093 1156 ApoI r'AATT_y Cuts at: 1493 3784 4010 4162 6271 1493 Size: 2291 226 152 2109 1697 Fragments arranged by size: 2291 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 2717 3014 3800 1870 Size: 73 774 297 786 4545 Fragments arranged by size: 4545 786 774 297 73 BamHI G'GATC_C Cuts at: 3805 4172 3805 Size: 367 6108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3596 3796 5251 618 Size: 325 217 627 1809 200 1455 1842 Fragments arranged by size: 1842 1809 1455 627 325 217 200 BanII G_rGCy'C Cuts at: 1391 3101 3521 3794 1391 Size: 1710 420 273 4072 Fragments arranged by size: 4072 1710 420 273 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 2910 928 Size: 971 240 771 4493 Fragments arranged by size: 4493 971 771 240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4666 4700 1903 Size: 34 2729 34 3678 Fragments arranged by size: 3678 2729 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4367 5577 4367 Size: 1210 5265 BclI T'GATC_A Cuts at: 1548 1548 Size: 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3042 5086 136 Size: 107 122 71 631 605 1370 2044 1525 Fragments arranged by size: 2044 1525 1370 631 605 122 107 71 BglII A'GATC_T Cuts at: 2935 3610 6470 2935 Size: 675 2860 2940 Fragments arranged by size: 2940 2860 675 BlpI GC'TnA_GC Cuts at: 3892 3892 Size: 6475 Bme1580I G_kGCm'C Cuts at: 1790 2443 3047 4536 5692 1790 Size: 653 604 1489 1156 2573 Fragments arranged by size: 2573 1489 1156 653 604 BmgBI CAC'GTC Cuts at: 1318 3433 1318 Size: 2115 4360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2306 3118 4206 Size: 416 53 83 399 24 889 812 1088 Cuts at: 4206 5164 442 Size: 958 1753 Fragments arranged by size: 1753 1088 958 889 812 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3244 3469 3244 Size: 225 6250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 2268 2501 3048 3230 3641 5135 1239 Size: 1016 13 233 547 182 411 1494 2579 Fragments arranged by size: 2579 1494 1016 547 411 233 182 13 Bpu10I CC'TnA_GC Cuts at: 3095 3095 Size: 6475 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4600 5465 5763 4600 Size: 865 298 5312 Fragments arranged by size: 5312 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2386 2759 3015 5138 882 Size: 617 887 373 256 2123 2219 Fragments arranged by size: 2219 2123 887 617 373 256 BsaAI yAC'GTr Cuts at: 493 1204 4266 493 Size: 711 3062 2702 Fragments arranged by size: 3062 2702 711 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3468 4282 4664 5299 Size: 53 83 186 936 1935 814 382 635 Cuts at: 5299 275 Size: 1451 Fragments arranged by size: 1935 1451 936 814 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2542 3631 3764 4908 5306 5580 5727 6138 2542 Size: 1089 133 1144 398 274 147 411 2879 Fragments arranged by size: 2879 1144 1089 411 398 274 147 133 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2366 2396 3209 3224 3239 Size: 30 165 30 963 30 813 15 15 Cuts at: 3239 3254 3269 3485 3515 1178 Size: 15 15 216 30 4138 Fragments arranged by size: 4138 963 813 216 165 30 30 30 30 15 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2408 2870 2873 3203 3233 3260 3326 1186 Size: 1222 462 3 330 30 27 66 4335 Fragments arranged by size: 4335 1222 462 330 66 30 27 3 BseYI C'CCAG_C Cuts at: 3298 3704 5678 6174 3298 Size: 406 1974 496 3599 Fragments arranged by size: 3599 1974 496 406 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5902 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3037 3166 3925 4686 5714 1540 Size: 275 186 1036 129 759 761 1028 2301 Fragments arranged by size: 2301 1036 1028 761 759 275 186 129 BsiHKAI G_wGCw'C Cuts at: 2443 4536 4621 5692 2443 Size: 2093 85 1071 3226 Fragments arranged by size: 3226 2093 1071 85 BsmI GAATG_Cn' Cuts at: 3566 3986 4079 3566 Size: 420 93 5962 Fragments arranged by size: 5962 420 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3206 3206 Size: 6475 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2443 3047 3101 3521 3794 4536 4621 Size: 399 653 604 54 420 273 742 85 Cuts at: 4621 5692 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 653 604 420 399 273 85 54 BspEI T'CCGG_A Cuts at: 3631 6138 3631 Size: 2507 3968 BspHI T'CATG_A Cuts at: 2799 4364 6094 2799 Size: 1565 1730 3180 Fragments arranged by size: 3180 1730 1565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3830 844 Size: 2986 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2863 4362 1394 Size: 429 1040 1499 3507 Fragments arranged by size: 3507 1499 1040 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4970 5144 65 Size: 1183 3722 174 1396 Fragments arranged by size: 3722 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2542 5119 5306 1700 Size: 110 732 2577 187 2869 Fragments arranged by size: 2869 2577 732 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6475 BssSI C'ACGA_G Cuts at: 2453 4535 5547 2453 Size: 2082 1012 3381 Fragments arranged by size: 3381 2082 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6475 BstBI TT'CG_AA Cuts at: 5279 5279 Size: 6475 BstEII G'GTnAC_C Cuts at: 1184 2545 2810 1184 Size: 1361 265 4849 Fragments arranged by size: 4849 1361 265 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6475 BstYI r'GATC_y Cuts at: 1972 2712 2886 2935 2982 3009 3610 3805 4172 Size: 740 174 49 47 27 601 195 367 Cuts at: 4172 4557 4574 6026 6112 6124 6470 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 740 601 385 367 346 195 174 86 49 47 27 17 12 Bsu36I CC'TnA_GG Cuts at: 2462 2462 Size: 6475 BtgI C'CryG_G Cuts at: 513 1065 2004 2262 2625 2691 3031 3160 3513 Size: 552 939 258 363 66 340 129 353 Cuts at: 3513 3533 513 Size: 20 3455 Fragments arranged by size: 3455 939 552 363 353 340 258 129 66 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5736 Fragments arranged by size: 5736 683 56 BtsI GCAGTG_nn' Cuts at: 3991 4785 4805 3991 Size: 794 20 5661 Fragments arranged by size: 5661 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3203 5482 809 Size: 507 1887 2279 1802 Fragments arranged by size: 2279 1887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2368 2971 Size: 54 1425 50 275 18 195 343 603 Cuts at: 2971 3034 3163 3922 4811 6440 8 Size: 63 129 759 889 1629 43 Fragments arranged by size: 1629 1425 889 759 603 343 275 195 129 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3034 3163 3922 1537 Size: 275 1222 129 759 4090 Fragments arranged by size: 4090 1222 759 275 129 EarI CTCTTCn'nnn_ Cuts at: 1353 2657 3671 4405 1353 Size: 1304 1014 734 3423 Fragments arranged by size: 3423 1304 1014 734 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2076 5062 5436 5582 387 Size: 1064 138 487 2986 374 146 1280 Fragments arranged by size: 2986 1280 1064 487 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2255 2268 2501 2837 3048 3230 Size: 393 494 129 13 233 336 211 182 Cuts at: 3230 3331 3641 4538 5135 5922 6190 1239 Size: 101 310 897 597 787 268 1524 Fragments arranged by size: 1524 897 787 597 494 393 336 310 268 233 211 182 129 101 13 EcoNI CCTnn'n_nnAGG Cuts at: 2720 3346 2720 Size: 626 5849 EcoO109I rG'GnC_Cy Cuts at: 3070 3112 3205 3070 Size: 42 93 6340 Fragments arranged by size: 6340 93 42 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 FspI TGC'GCA Cuts at: 4981 4981 Size: 6475 HaeII r_GCGC'y Cuts at: 1678 2653 3370 5622 1678 Size: 975 717 2252 2531 Fragments arranged by size: 2531 2252 975 717 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 3012 3044 3134 Size: 32 135 32 794 32 614 32 90 Cuts at: 3134 3166 3239 3271 3485 3517 5116 5148 5190 Size: 32 73 32 214 32 1599 32 42 Cuts at: 5190 5222 1373 Size: 32 2626 Fragments arranged by size: 2626 1599 794 614 214 135 90 73 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2728 2746 3819 4065 669 Size: 988 281 790 18 1073 246 3079 Fragments arranged by size: 3079 1073 988 790 281 246 18 HindIII A'AGCT_T Cuts at: 748 748 Size: 6475 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KpnI G_GTAC'C Cuts at: 3600 3800 3600 Size: 200 6275 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2511 3366 5589 5773 1305 Size: 1206 855 2223 184 2007 Fragments arranged by size: 2223 2007 1206 855 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2370 2973 10 Size: 54 1425 343 195 343 603 3512 Fragments arranged by size: 3512 1425 603 343 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 4433 4792 4951 518 Size: 648 3267 359 159 2042 Fragments arranged by size: 3267 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3033 3162 3891 4568 5716 5961 1606 Size: 1427 129 729 677 1148 245 2120 Fragments arranged by size: 2120 1427 1148 729 677 245 129 NcoI C'CATG_G Cuts at: 513 1065 2004 2691 3533 513 Size: 552 939 687 842 3455 Fragments arranged by size: 3455 939 842 687 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3163 3922 3163 Size: 759 5716 NspI r_CATG'y Cuts at: 1132 1229 2500 3833 5378 1132 Size: 97 1271 1333 1545 2229 Fragments arranged by size: 2229 1545 1333 1271 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6475 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 3534 1108 Size: 1269 1157 4049 Fragments arranged by size: 4049 1269 1157 PfoI T'CCnGG_A Cuts at: 2253 3207 2253 Size: 954 5521 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4544 4576 6057 6089 4544 Size: 32 1481 32 4930 Fragments arranged by size: 4930 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3070 3205 3070 Size: 135 6340 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6475 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2912 2944 2912 Size: 32 6443 PstI C_TGCA'G Cuts at: 830 2168 3325 3827 830 Size: 1338 1157 502 3478 Fragments arranged by size: 3478 1338 1157 502 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 SacII CC_GC'GG Cuts at: 3034 3163 3034 Size: 129 6346 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SbfI CC_TGCA'GG Cuts at: 3827 3827 Size: 6475 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SexAI A'CCwGG_T Cuts at: 2376 2376 Size: 6475 SfcI C'TryA_G Cuts at: 826 1045 2164 2405 3321 3823 4958 5639 5830 Size: 219 1119 241 916 502 1135 681 191 Cuts at: 5830 826 Size: 1471 Fragments arranged by size: 1471 1135 1119 916 681 502 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6475 SmaI CCC'GGG Cuts at: 1872 3802 1872 Size: 1930 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2717 4579 5480 5742 820 Size: 197 926 774 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 774 262 197 SnaBI TAC'GTA Cuts at: 493 4266 493 Size: 3773 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 3833 1132 Size: 2701 3774 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 2227 1517 Size: 710 5765 StyI C'CwwG_G Cuts at: 513 1065 2004 2691 2769 3115 3533 3914 513 Size: 552 939 687 78 346 418 381 3074 Fragments arranged by size: 3074 939 687 552 418 381 346 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4502 4519 4672 4857 5302 325 Size: 1255 2922 17 153 185 445 1498 Fragments arranged by size: 2922 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3225 3420 4721 Size: 274 80 33 51 1443 1248 195 1301 Cuts at: 4721 6433 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1301 1248 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2433 2465 2765 2797 3224 3256 6435 6467 2433 Size: 32 300 32 427 32 3179 32 2441 Fragments arranged by size: 3179 2441 427 300 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2378 1316 Size: 1062 5413 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2230 2230 Size: 6475 XhoI C'TCGA_G Cuts at: 1943 2717 1943 Size: 774 5701 XmaI C'CCGG_G Cuts at: 1870 3800 1870 Size: 1930 4545 XmnI GAAnn'nnTTC Cuts at: 4604 4604 Size: 6475 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI