Restriction map for
pFN21AE6362
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BalI BamHI
BbrPI BcgI BclI BcnI BfrI
BglII BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HapII HincII
HindII HpaI HpaII KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NciI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 637 GTCTAC GT^[AC][GT]AC
AcsI 962 AAATTC [GA]^AATT[CT]
AflIII 641 ACATGT A^C[GA][CT]GT
Alw44I 207 GTGCAC G^TGCAC
AocI 409 CCTCAGG CC^T[ACGT]AGG
ApaLI 207 GTGCAC G^TGCAC
ApoI 962 AAATTC [GA]^AATT[CT]
AspHI 207 GTGCAC G[AT]GC[AT]^C
AspI 55 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 192 CTCGAG C^[CT]CG[GA]G
AvrII 42 CCTAGG C^CTAGG
BanII 76 GAGCCC G[GA]GC[CT]^C
BbeI 351 GGCGCC GGCGC^C
BbsI 796 GTCTTC GAAGAC(2/6)
BbvCI 776 CCTCAGC CCTCAGC(-5/-2)
BciVI 251 GGATAC GTATCC(6/5)
BlnI 42 CCTAGG C^CTAGG
BpuAI 796 GTCTTC GAAGAC(2/6)
BseRI 16 GAGGAG GAGGAG(10/8)
BsiHKAI 207 GTGCAC G[AT]GC[AT]^C
BsmBI 53 GAGACG CGTCTC(1/5)
BsoBI 192 CTCGAG C^[CT]CG[GA]G
BspLU11I 641 ACATGT A^CATGT
BspMI 327 ACCTGC ACCTGC(4/8)
BssSI 613 CTCGTG CACGAG(-5/-1)
BstYI 150 AGATCC [GA]^GATC[CT]
Bsu36I 409 CCTCAGG CC^T[ACGT]AGG
DraI 811 TTTAAA TTT^AAA
DsaI 356 CCACGG C^C[GA][CT]GG
EarI 955 GAAGAG CTCTTC(1/4)
Eco81I 409 CCTCAGG CC^T[ACGT]AGG
EcoNI 6 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 351 GGCGCC G^GCGCC
Ksp632I 955 GAAGAG CTCTTC(1/4)
MaeII 83 ACGT A^CGT
MaeIII 467 GTTAC ^GT[ACGT]AC
MflI 150 AGATCC [GA]^GATC[CT]
NarI 351 GGCGCC GG^CGCC
NheI 747 GCTAGC G^CTAGC
NspI 641 ACATGT [GA]CATG^[CT]
PaeR7I 192 CTCGAG C^TCGAG
PflFI 55 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 922 CCACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 329 CTGCAG CTGCA^G
ScaI 941 AGTACT AGT^ACT
SfiI 568 GGCCTGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 351 GGCGCC GGC^GCC
SmlI 192 CTCGAG C^T[CT][GA]AG
Tth111I 55 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 922 CCACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 192 CTCGAG C^TCGAG
XhoII 150 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 872 GTAC GT^AC
942 GTAC
BanI 351 GGCGCC G^G[CT][GA]CC
897 GGTGCC
BglI 427 GCCTACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
569 GCCTGACTGGC
BmyI 76 GAGCCC G[AGT]GC[ACT]^C
207 GTGCAC
BsmAI 53 GAGAC GTCTC(1/5)
969 GTCTC
Bsp1286I 76 GAGCCC G[AGT]GC[ACT]^C
207 GTGCAC
BsrDI 695 GCAATG GCAATG(2/0)
883 GCAATG
BstF5I 289 CATCC GGATG(2/0)
752 CATCC
Cac8I 618 GCAAGC GC[ACGT]^[ACGT]GC
747 GCTAGC
DraII 772 GGGACCT [GA]G^G[ACGT]CC[CT]
817 AGGACCC
EcoO109I 772 GGGACCT [GA]G^G[ACGT]CC[CT]
817 AGGACCC
EcoT14I 42 CCTAGG C^C[AT][AT]GG
580 CCTTGG
FauI 64 CCCGC CCCGC(4/6)
505 GCGGG
FokI 289 CATCC GGATG(9/13)
752 CATCC
HaeII 104 AGCGCC [GA]GCGC^[CT]
351 GGCGCC
HaeIII 568 GGCC GG^CC
577 GGCC
HindIII 260 AAGCTT A^AGCTT
316 AAGCTT
HphI 98 TCACC GGTGA(8/7)
800 TCACC
PleI 593 GACTC GAGTC(4/5)
273 GAGTC
PpuMI 772 GGGACCT [GA]G^G[AT]CC[CT]
817 AGGACCC
RsaI 872 GTAC GT^AC
942 GTAC
SfcI 329 CTGCAG C^T[GA][CT]AG
945 CTGTAG
StyI 42 CCTAGG C^C[AT][AT]GG
580 CCTTGG
TfiI 658 GAATC G^A[AT]TC
786 GATTC
XcmI 555 CCAGATGAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
841 CCAGGGTCATTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcyI AluI AlwI AvaII
BbvI BfaI BsaHI BsaJI BsiYI
BslI BsmFI BsrI BssKI BstNI
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI HgaI HhaI Hin1I HinP1I
HinfI ItaI MaeI MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I