(Circular) (Six-base) MAPSORT of: pFN21AE6362.seq Check: 1661 from: 1 to: 5716 pFN21AE6362 5716 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2643 3059 1937 Size: 706 416 4594 Fragments arranged by size: 4594 706 416 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2817 3779 5163 5431 1632 Size: 1185 962 1384 268 1917 Fragments arranged by size: 1917 1384 1185 962 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 2646 4615 5592 1203 Size: 1443 1969 977 1327 Fragments arranged by size: 1969 1443 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 3459 5031 3459 Size: 1572 4144 ApaLI G'TGCA_C Cuts at: 2212 3773 4929 2212 Size: 1561 1156 2999 Fragments arranged by size: 2999 1561 1156 ApoI r'AATT_y Cuts at: 1493 2967 3025 3251 3403 5512 1493 Size: 1474 58 226 152 2109 1697 Fragments arranged by size: 2109 1697 1474 226 152 58 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 2197 3041 1870 Size: 73 254 844 4545 Fragments arranged by size: 4545 844 254 73 AvrII C'CTAG_G Cuts at: 2047 2047 Size: 5716 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2356 2902 3037 4492 618 Size: 325 217 627 569 546 135 1455 1842 Fragments arranged by size: 1842 1455 627 569 546 325 217 135 BanII G_rGCy'C Cuts at: 1391 2085 3035 1391 Size: 694 950 4072 Fragments arranged by size: 4072 950 694 BbeI G_GCGC'C Cuts at: 2360 2360 Size: 5716 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2794 928 Size: 971 895 3850 Fragments arranged by size: 3850 971 895 BbvCI CC'TCA_GC Cuts at: 2782 2782 Size: 5716 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2250 3608 4818 2250 Size: 1358 1210 3148 Fragments arranged by size: 3148 1358 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2438 2580 4327 Size: 107 122 71 631 605 766 142 1747 Cuts at: 4327 136 Size: 1525 Fragments arranged by size: 1747 1525 766 631 605 142 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 3133 3133 Size: 5716 Bme1580I G_kGCm'C Cuts at: 1790 2216 3777 4933 1790 Size: 426 1561 1156 2573 Fragments arranged by size: 2573 1561 1156 426 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2821 3447 4405 Size: 416 53 83 399 24 1404 626 958 Cuts at: 4405 442 Size: 1753 Fragments arranged by size: 1753 1404 958 626 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2756 1056 Size: 1700 4016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4376 1239 Size: 3137 2579 Bpu10I CC'TnA_GC Cuts at: 2782 2782 Size: 5716 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3841 4706 5004 3841 Size: 865 298 4553 Fragments arranged by size: 4553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 882 Size: 617 2880 2219 Fragments arranged by size: 2880 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3507 493 Size: 711 2303 2702 Fragments arranged by size: 2702 2303 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2357 2381 3523 Size: 53 83 186 936 528 296 24 1142 Cuts at: 3523 3905 4540 275 Size: 382 635 1451 Fragments arranged by size: 1451 1142 936 635 528 382 296 186 83 53 24 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5491 Fragments arranged by size: 5491 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 1186 Size: 850 4866 BseYI C'CCAG_C Cuts at: 2726 4919 5415 2726 Size: 2193 496 3027 Fragments arranged by size: 3027 2193 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5143 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3166 3927 4955 1540 Size: 275 186 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2216 3777 3862 4933 2216 Size: 1561 85 1071 2999 Fragments arranged by size: 2999 1561 1071 85 BsmI GAATG_Cn' Cuts at: 3227 3320 3227 Size: 93 5623 BsmBI CGTCTCn'nnnn_ Cuts at: 2052 2052 Size: 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2216 3035 3777 3862 4933 1391 Size: 399 295 131 819 742 85 1071 2174 Fragments arranged by size: 2174 1071 819 742 399 295 131 85 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2341 3071 844 Size: 1497 730 3489 Fragments arranged by size: 3489 1497 730 BsrBI CCG'CTC Cuts at: 1394 1823 3603 1394 Size: 429 1780 3507 Fragments arranged by size: 3507 1780 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2707 2895 4211 4385 65 Size: 1183 1459 188 1316 174 1396 Fragments arranged by size: 1459 1396 1316 1183 188 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4360 4547 1700 Size: 110 2550 187 2869 Fragments arranged by size: 2869 2550 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 2618 3776 4788 2618 Size: 1158 1012 3546 Fragments arranged by size: 3546 1158 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5716 BstYI r'GATC_y Cuts at: 1972 2155 3046 3413 3798 3815 5267 5353 5365 Size: 183 891 367 385 17 1452 86 12 Cuts at: 5365 5711 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 891 385 367 346 183 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2415 2415 Size: 5716 BtgI C'CryG_G Cuts at: 513 1065 2361 513 Size: 552 1296 3868 Fragments arranged by size: 3868 1296 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 3232 4026 4046 3232 Size: 794 20 4902 Fragments arranged by size: 4902 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 2818 3020 2818 Size: 202 5514 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3163 4052 5681 Size: 54 1425 50 275 18 1333 889 1629 Cuts at: 5681 8 Size: 43 Fragments arranged by size: 1629 1425 1333 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2955 3646 1353 Size: 1602 691 3423 Fragments arranged by size: 3423 1602 691 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2261 4303 4677 4823 387 Size: 1064 138 672 2042 374 146 1280 Fragments arranged by size: 2042 1280 1064 672 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2817 3779 4376 5163 5431 1239 Size: 393 1185 962 597 787 268 1524 Fragments arranged by size: 1524 1185 962 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2015 Size: 5716 EcoO109I rG'GnC_Cy Cuts at: 2778 2823 2778 Size: 45 5671 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2493 2493 Size: 5716 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 2113 2360 4863 1678 Size: 435 247 2503 2531 Fragments arranged by size: 2531 2503 435 247 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2049 2081 2099 2131 2398 Size: 32 135 32 477 32 18 32 267 Cuts at: 2398 2430 2770 2802 4357 4389 4431 4463 1373 Size: 32 340 32 1555 32 42 32 2626 Fragments arranged by size: 2626 1555 477 340 267 135 42 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3306 669 Size: 988 281 1122 246 3079 Fragments arranged by size: 3079 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 2265 2321 748 Size: 1517 56 4143 Fragments arranged by size: 4143 1517 56 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KasI G'GCGC_C Cuts at: 2356 2356 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3002 4830 5014 1305 Size: 1697 1828 184 2007 Fragments arranged by size: 2007 1828 1697 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3674 4033 4192 518 Size: 648 2508 359 159 2042 Fragments arranged by size: 2508 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3132 3809 4957 5202 1606 Size: 1526 677 1148 245 2120 Fragments arranged by size: 2120 1526 1148 677 245 NarI GG'CG_CC Cuts at: 2357 2357 Size: 5716 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5164 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NheI G'CTAG_C Cuts at: 1052 2752 1052 Size: 1700 4016 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 2650 3074 4619 1132 Size: 97 1421 424 1545 2229 Fragments arranged by size: 2229 1545 1421 424 97 PasI CC'CwG_GG Cuts at: 1348 2846 1348 Size: 1498 4218 PciI A'CATG_T Cuts at: 2646 4615 2646 Size: 1969 3747 PflMI CCAn_nnn'nTGG Cuts at: 1108 2933 1108 Size: 1825 3891 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2778 2823 2778 Size: 45 5671 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PspXI vC'TCGA_Gb Cuts at: 2197 2197 Size: 5716 PstI C_TGCA'G Cuts at: 830 2338 3068 830 Size: 1508 730 3478 Fragments arranged by size: 3478 1508 730 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 2948 3964 5676 2948 Size: 1016 1712 2988 Fragments arranged by size: 2988 1712 1016 SfcI C'TryA_G Cuts at: 826 1045 2334 2950 3064 4199 4880 5071 826 Size: 219 1289 616 114 1135 681 191 1471 Fragments arranged by size: 1471 1289 1135 681 616 219 191 114 SfiI GGCCn_nnn'nGGCC Cuts at: 2580 2580 Size: 5716 SfoI GGC'GCC Cuts at: 2358 2358 Size: 5716 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2197 3820 4721 4983 820 Size: 197 926 254 1623 901 262 1553 Fragments arranged by size: 1623 1553 926 901 262 254 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5716 StyI C'CwwG_G Cuts at: 513 1065 2047 2585 3155 513 Size: 552 982 538 570 3074 Fragments arranged by size: 3074 982 570 552 538 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3743 3760 3913 4098 4543 325 Size: 1255 2163 17 153 185 445 1498 Fragments arranged by size: 2163 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2876 2946 3962 Size: 274 80 33 51 1443 899 70 1016 Cuts at: 3962 5674 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1016 899 274 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2886 2918 5676 5708 2886 Size: 32 2758 32 2894 Fragments arranged by size: 2894 2758 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2063 1316 Size: 747 4969 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2567 2853 2567 Size: 286 5430 XhoI C'TCGA_G Cuts at: 1943 2197 1943 Size: 254 5462 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 3845 3845 Size: 5716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SrfI SwaI