################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MamI MfeI MflI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SalI SapI SbfI ScaI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcyI 815 GGCGCC G[GA]^CG[CT]C AlwNI 151 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 21 GGGCCC GGGCC^C AvaI 17 CTCGGG C^[CT]CG[GA]G AviII 1037 TGCGCA TGC^GCA BbeI 815 GGCGCC GGCGC^C BbrPI 598 CACGTG CAC^GTG BcgI 88 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 544 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 131 CTCCAG CTGGAG(16/14) BsaAI 598 CACGTG [CT]AC^GT[GA] BsaHI 815 GGCGCC G[GA]^CG[CT]C BsoBI 17 CTCGGG C^[CT]CG[GA]G BsrBI 343 CCGCTC CCGCTC(-3/-3) BstF5I 720 CATCC GGATG(2/0) DrdI 472 GACCGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 627 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1077 CCATGG C^C[AT][AT]GG FokI 720 CATCC GGATG(9/13) FspI 1037 TGCGCA TGC^GCA Hin1I 815 GGCGCC G[GA]^CG[CT]C KasI 815 GGCGCC G^GCGCC KspI 560 CCGCGG CCGC^GG MslI 462 CATCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 815 GGCGCC GG^CGCC NcoI 1077 CCATGG C^CATGG PmaCI 598 CACGTG CAC^GTG PmlI 598 CACGTG CAC^GTG PpuMI 902 GGGACCT [GA]G^G[AT]CC[CT] SacI 604 GAGCTC GAGCT^C SacII 560 CCGCGG CCGC^GG SexAI 404 ACCTGGT A^CC[AT]GGT SfoI 815 GGCGCC GGC^GCC SmlI 607 CTCAAG C^T[CT][GA]AG StuI 92 AGGCCT AGG^CCT StyI 1077 CCATGG C^C[AT][AT]GG XcmI 458 CCACCATCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### BalI 542 TGGCCA TGG^CCA 1080 TGGCCA BanI 768 GGTGCC G^G[CT][GA]CC 815 GGCGCC BbsI 409 GTCTTC GAAGAC(2/6) 886 GTCTTC BbvCI 522 CCTCAGC CCTCAGC(-5/-2) 498 GCTGAGG BcnI 738 CCGGG CC^[GC]GG 772 CCGGG BpuAI 409 GTCTTC GAAGAC(2/6) 886 GTCTTC BseRI 634 GAGGAG GAGGAG(10/8) 1030 GAGGAG BsmFI 803 GGGAC GGGAC(10/14) 902 GGGAC BspMI 701 ACCTGC ACCTGC(4/8) 893 ACCTGC BsrI 800 ACTGG ACTGG(1/-1) 962 ACTGG BstEII 426 GGTCACC G^GT[ACGT]ACC 693 GGTCACC BstPI 426 GGTCACC G^GT[ACGT]ACC 693 GGTCACC BstXI 370 CCACGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 461 CCATCGCAGTGG DdeI 499 CTGAG C^T[ACGT]AG 523 CTCAG DpnII 186 GATC ^GATC 252 GATC DraIII 274 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG 616 CACGACGTG DsaI 560 CCGCGG C^C[GA][CT]GG 1077 CCATGG EaeI 542 TGGCCA [CT]^GGCC[GA] 1080 TGGCCA EcoO65I 426 GGTCACC G^GT[ACGT]ACC 693 GGTCACC HgaI 127 GACGC GACGC(5/10) 203 GCGTC MboI 186 GATC ^GATC 252 GATC MluNI 542 TGGCCA TGG^CCA 1080 TGGCCA MscI 542 TGGCCA TGG^CCA 1080 TGGCCA NciI 738 CCGGG CC^[GC]GG 772 CCGGG NdeII 186 GATC ^GATC 252 GATC PstI 157 CTGCAG CTGCA^G 418 CTGCAG Sau3AI 186 GATC ^GATC 252 GATC SfaNI 168 GCATC GCATC(5/9) 509 GCATC SgrAI 56 CGCCGGCG C[GA]^CCGG[CT]G 817 CGCCGGCG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AluI Alw44I ApaLI AspHI AvaII BanII BbvI BmyI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BsrFI BssKI BstAPI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NgoMIV NlaIII NlaIV PvuII Sau96I ScrFI SfcI TaqI TseI Tsp45I TspRI TthHB8I