(Circular) (Six-base) MAPSORT of: pFN21AE9515.seq Check: 6321 from: 1 to: 5815 pFN21AE9515 5815 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 3878 5262 5530 1632 Size: 569 1677 1384 268 1917 Fragments arranged by size: 1917 1677 1384 569 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 4714 5691 1203 Size: 3511 977 1327 Fragments arranged by size: 3511 1327 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5815 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2258 2537 1184 Size: 1074 279 4462 Fragments arranged by size: 4462 1074 279 AlwNI CAG_nnn'CTG Cuts at: 2161 3558 5130 2161 Size: 1397 1572 2846 Fragments arranged by size: 2846 1572 1397 ApaI G_GGCC'C Cuts at: 2030 2030 Size: 5815 ApaLI G'TGCA_C Cuts at: 2332 2495 2591 3872 5028 2332 Size: 163 96 1281 1156 3119 Fragments arranged by size: 3119 1281 1156 163 96 ApoI r'AATT_y Cuts at: 1493 3124 3350 3502 5611 1493 Size: 1631 226 152 2109 1697 Fragments arranged by size: 2109 1697 1631 226 152 AseI AT'TA_AT Cuts at: 160 1032 4369 160 Size: 872 3337 1606 Fragments arranged by size: 3337 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 2022 3140 1870 Size: 73 79 1118 4545 Fragments arranged by size: 4545 1118 79 73 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2773 2820 3136 4591 618 Size: 325 217 627 986 47 316 1455 1842 Fragments arranged by size: 1842 1455 986 627 325 316 217 47 BanII G_rGCy'C Cuts at: 1391 2030 2613 2654 3134 1391 Size: 639 583 41 480 4072 Fragments arranged by size: 4072 639 583 480 41 BbeI G_GCGC'C Cuts at: 2824 2824 Size: 5815 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2407 2884 928 Size: 971 508 477 3859 Fragments arranged by size: 3859 971 508 477 BbvCI CC'TCA_GC Cuts at: 2504 2528 2504 Size: 24 5791 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2082 2116 4006 4040 1903 Size: 34 145 34 1890 34 3678 Fragments arranged by size: 3678 1890 145 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2555 4426 136 Size: 107 122 71 631 605 883 1871 1525 Fragments arranged by size: 1871 1525 883 631 605 122 107 71 BglII A'GATC_T Cuts at: 5810 5810 Size: 5815 BlpI GC'TnA_GC Cuts at: 3232 3232 Size: 5815 Bme1580I G_kGCm'C Cuts at: 1790 2030 2336 2499 2595 3876 5032 1790 Size: 240 306 163 96 1281 1156 2573 Fragments arranged by size: 2573 1281 1156 306 240 163 96 BmgBI CAC'GTC Cuts at: 1318 2323 2626 2890 1318 Size: 1005 303 264 4243 Fragments arranged by size: 4243 1005 303 264 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2815 3546 4504 Size: 416 53 83 399 24 1398 731 958 Cuts at: 4504 442 Size: 1753 Fragments arranged by size: 1753 1398 958 731 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2121 4475 1239 Size: 882 2354 2579 Fragments arranged by size: 2579 2354 882 Bpu10I CC'TnA_GC Cuts at: 2504 2528 2504 Size: 24 5791 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2597 3940 4805 5103 2597 Size: 1343 865 298 3309 Fragments arranged by size: 3309 1343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2605 3606 493 Size: 711 1401 1001 2702 Fragments arranged by size: 2702 1401 1001 711 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2821 3622 4004 4639 Size: 53 83 186 936 1288 801 382 635 Cuts at: 4639 275 Size: 1451 Fragments arranged by size: 1451 1288 936 801 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4248 4646 4920 5067 5478 4248 Size: 398 274 147 411 4585 Fragments arranged by size: 4585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5590 Fragments arranged by size: 5590 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 3050 1186 Size: 1468 396 3951 Fragments arranged by size: 3951 1468 396 BseYI C'CCAG_C Cuts at: 2382 5018 5514 2382 Size: 2636 496 2683 Fragments arranged by size: 2683 2636 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 2870 2972 3002 1163 Size: 573 525 609 102 30 3976 Fragments arranged by size: 3976 609 573 525 102 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3265 4026 5054 1540 Size: 275 186 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 2336 2499 2595 2613 3876 3961 5032 2328 Size: 8 163 96 18 1263 85 1071 3111 Fragments arranged by size: 3111 1263 1071 163 96 85 18 8 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2328 2336 2499 2595 2613 2654 Size: 399 240 298 8 163 96 18 41 Cuts at: 2654 3134 3876 3961 5032 1391 Size: 480 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 480 399 298 240 163 96 85 41 18 8 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 3704 5434 3704 Size: 1730 4085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2715 2907 3170 844 Size: 1871 192 263 3489 Fragments arranged by size: 3489 1871 263 192 BsrBI CCG'CTC Cuts at: 1394 1823 2350 3702 1394 Size: 429 527 1352 3507 Fragments arranged by size: 3507 1352 527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4310 4484 65 Size: 1183 3062 174 1396 Fragments arranged by size: 3062 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2271 2428 2823 4459 4646 1700 Size: 110 252 209 157 395 1636 187 2869 Fragments arranged by size: 2869 1636 395 252 209 187 157 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5815 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5815 BssSI C'ACGA_G Cuts at: 3875 4887 3875 Size: 1012 4803 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 2340 2626 2835 794 Size: 1376 170 286 209 3774 Fragments arranged by size: 3774 1376 286 209 170 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 2431 2698 1184 Size: 1247 267 4301 Fragments arranged by size: 4301 1247 267 BstXI CCAn_nnnn'nTGG Cuts at: 2382 2473 2382 Size: 91 5724 BstYI r'GATC_y Cuts at: 1972 3145 3512 3897 3914 5366 5452 5464 5810 Size: 1173 367 385 17 1452 86 12 346 Cuts at: 5810 1972 Size: 1977 Fragments arranged by size: 1977 1452 1173 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2565 3082 513 Size: 552 939 561 517 3246 Fragments arranged by size: 3246 939 561 552 517 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2452 487 Size: 683 56 1226 3850 Fragments arranged by size: 3850 1226 683 56 BtsI GCAGTG_nn' Cuts at: 2478 3331 4125 4145 2478 Size: 853 794 20 4148 Fragments arranged by size: 4148 853 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 3119 3119 Size: 5815 DraIII CAC_nnn'GTG Cuts at: 2284 2626 2284 Size: 342 5473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2483 4822 809 Size: 507 1167 2339 1802 Fragments arranged by size: 2339 1802 1167 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2547 3085 3262 Size: 54 1425 50 275 18 717 538 177 Cuts at: 3262 4151 5780 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 717 538 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3262 1537 Size: 275 1450 4090 Fragments arranged by size: 4090 1450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3745 1353 Size: 2392 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4402 4776 4922 387 Size: 1064 138 2813 374 146 1280 Fragments arranged by size: 2813 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2611 2611 Size: 5815 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2201 3878 4475 5262 5530 1239 Size: 393 489 80 1677 597 787 268 1524 Fragments arranged by size: 1677 1524 787 597 489 393 268 80 EcoNI CCTnn'n_nnAGG Cuts at: 2636 2636 Size: 5815 EcoO109I rG'GnC_Cy Cuts at: 2026 2027 2908 2026 Size: 1 881 4933 Fragments arranged by size: 4933 881 1 EcoRI G'AATT_C Cuts at: 3124 3502 3124 Size: 378 5437 FspI TGC'GCA Cuts at: 3044 4321 3044 Size: 1277 4538 HaeII r_GCGC'y Cuts at: 1678 2535 2561 2579 2665 2671 2824 4962 1678 Size: 857 26 18 86 6 153 2138 2531 Fragments arranged by size: 2531 2138 857 153 86 26 18 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4456 4488 4530 4562 1373 Size: 32 135 32 2884 32 42 32 2626 Fragments arranged by size: 2884 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3405 669 Size: 988 281 1221 246 3079 Fragments arranged by size: 3079 1221 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KasI G'GCGC_C Cuts at: 2820 2820 Size: 5815 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 3414 3414 Size: 5815 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2343 4929 5113 1305 Size: 1038 2586 184 2007 Fragments arranged by size: 2586 2007 1038 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2549 3087 10 Size: 54 1425 343 717 538 2738 Fragments arranged by size: 2738 1425 717 538 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2471 3773 4132 4291 518 Size: 648 1305 1302 359 159 2042 Fragments arranged by size: 2042 1305 1302 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2167 2179 2503 2567 2884 2956 3231 3908 Size: 561 12 324 64 317 72 275 677 Cuts at: 3908 5056 5301 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 561 324 317 275 245 72 64 12 NaeI GCC'GGC Cuts at: 2064 2273 2825 2064 Size: 209 552 5054 Fragments arranged by size: 5054 552 209 NarI GG'CG_CC Cuts at: 2821 2821 Size: 5815 NcoI C'CATG_G Cuts at: 513 1065 2004 3082 513 Size: 552 939 1078 3246 Fragments arranged by size: 3246 1078 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NgoMIV G'CCGG_C Cuts at: 2062 2271 2823 2062 Size: 209 552 5054 Fragments arranged by size: 5054 552 209 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5815 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 3173 4718 1132 Size: 97 1944 1545 2229 Fragments arranged by size: 2229 1944 1545 97 PasI CC'CwG_GG Cuts at: 1348 2031 1348 Size: 683 5132 PciI A'CATG_T Cuts at: 4714 4714 Size: 5815 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5815 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PmlI CAC'GTG Cuts at: 2605 2605 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2908 2908 Size: 5815 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PspOMI G'GGCC_C Cuts at: 2026 2026 Size: 5815 PstI C_TGCA'G Cuts at: 830 2166 2427 3167 830 Size: 1336 261 740 3478 Fragments arranged by size: 3478 1336 740 261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 PvuII CAG'CTG Cuts at: 2167 2179 2956 2167 Size: 12 777 5026 Fragments arranged by size: 5026 777 12 SacI G_AGCT'C Cuts at: 2613 2613 Size: 5815 SacII CC_GC'GG Cuts at: 2568 2568 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SexAI A'CCwGG_T Cuts at: 2409 2409 Size: 5815 SfcI C'TryA_G Cuts at: 826 1045 2162 2203 2423 3163 4298 4979 5170 Size: 219 1117 41 220 740 1135 681 191 Cuts at: 5170 826 Size: 1471 Fragments arranged by size: 1471 1135 1117 740 681 220 219 191 41 SfoI GGC'GCC Cuts at: 2822 2822 Size: 5815 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 2062 2823 1700 Size: 362 761 4692 Fragments arranged by size: 4692 761 362 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2612 3919 4820 5082 820 Size: 197 926 669 1307 901 262 1553 Fragments arranged by size: 1553 1307 926 901 669 262 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 3739 5 Size: 47 3687 2081 Fragments arranged by size: 3687 2081 47 StuI AGG'CCT Cuts at: 1517 2099 1517 Size: 582 5233 StyI C'CwwG_G Cuts at: 513 1065 2004 3082 3254 513 Size: 552 939 1078 172 3074 Fragments arranged by size: 3074 1078 939 552 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2374 3842 3859 4012 4197 4642 325 Size: 1255 794 1468 17 153 185 445 1498 Fragments arranged by size: 1498 1468 1255 794 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4061 5773 96 Size: 274 80 33 51 1443 2084 1712 138 Fragments arranged by size: 2084 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5775 5807 5775 Size: 32 5783 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5815 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2470 2470 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5815 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 3944 3944 Size: 5815 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI