################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BbsI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I TspRI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AfaI 369 GTAC GT^AC Aor51HI 359 AGCGCT AGC^GCT AspHI 394 GAGCTC G[AT]GC[AT]^C AvaI 56 CCCGGG C^[CT]CG[GA]G BamHI 227 GGATCC G^GATCC BanI 145 GGCGCC G^G[CT][GA]CC BanII 394 GAGCTC G[GA]GC[CT]^C BbeI 145 GGCGCC GGCGC^C BbvCI 169 GCTGAGG CCTCAGC(-5/-2) BcgI 330 GCAGCTCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 394 GAGCTC G[AGT]GC[ACT]^C BpmI 343 CTGGAG CTGGAG(16/14) BsaAI 370 TACGTG [CT]AC^GT[GA] BsiHKAI 394 GAGCTC G[AT]GC[AT]^C BsmFI 527 GGGAC GGGAC(10/14) BsoBI 56 CCCGGG C^[CT]CG[GA]G Bsp1286I 394 GAGCTC G[AGT]GC[ACT]^C BsrFI 293 ACCGGC [GA]^CCGG[CT] BsrI 366 CCAGT ACTGG(1/-1) BstXI 275 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 227 GGATCC [GA]^GATC[CT] Cfr10I 293 ACCGGC [GA]^CCGG[CT] DraII 278 AGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 306 GACGCGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 323 CCGCGG C^C[GA][CT]GG Eco47III 359 AGCGCT AGC^GCT EcoNI 270 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 278 AGGGCCT [GA]G^G[ACGT]CC[CT] FauI 464 GCGGG CCCGC(4/6) HphI 320 TCACC GGTGA(8/7) KasI 145 GGCGCC G^GCGCC KspI 323 CCGCGG CCGC^GG MaeIII 179 GTAAC ^GT[ACGT]AC MflI 227 GGATCC [GA]^GATC[CT] MseI 410 TTAA T^TAA NarI 145 GGCGCC GG^CGCC PflMI 366 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 369 GTAC GT^AC SacI 394 GAGCTC GAGCT^C SacII 323 CCGCGG CCGC^GG SfoI 145 GGCGCC GGC^GCC SmaI 56 CCCGGG CCC^GGG SmlI 130 CTCAAG C^T[CT][GA]AG TfiI 455 GATTC G^A[AT]TC Tru9I 410 TTAA T^TAA Tsp509I 196 AATT ^AATT Van91I 366 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 227 GGATCC [GA]^GATC[CT] XmaCI 56 CCCGGG C^CCGGG XmaI 56 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 43 GACGCC G[GA]^CG[CT]C 145 GGCGCC AlwI 228 GATCC GGATC(4/5) 227 GGATC BsaHI 43 GACGCC G[GA]^CG[CT]C 145 GGCGCC BsaI 421 GAGACC GGTCTC(1/5) 60 GGTCTC BstF5I 255 CATCC GGATG(2/0) 267 CATCC Cac8I 396 GCTCGC GC[ACGT]^[ACGT]GC 501 GCCAGC DpnII 228 GATC ^GATC 264 GATC EcoT14I 245 CCAAGG C^C[AT][AT]GG 275 CCAAGG FokI 255 CATCC GGATG(9/13) 267 CATCC Hin1I 43 GACGCC G[GA]^CG[CT]C 145 GGCGCC MboI 228 GATC ^GATC 264 GATC MspA1I 203 CAGCGG C[AC]G^C[GT]G 323 CCGCGG NdeII 228 GATC ^GATC 264 GATC Sau3AI 228 GATC ^GATC 264 GATC SfaNI 32 GATGC GCATC(5/9) 241 GATGC StyI 245 CCAAGG C^C[AT][AT]GG 275 CCAAGG TaqI 30 TCGA T^CGA 64 TCGA TthHB8I 30 TCGA T^CGA 64 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AccII AciI AluI AvaII BbvI BcnI BsaJI BsiYI BslI BsmAI BssKI BstNI BstUI CfoI Cfr13I DdeI EcoRII Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MaeII MboII MnlI MspI MvaI MvnI MwoI NciI NlaIV PleI Sau96I ScrFI TseI