Restriction map for
pFN21AE5229
(Circular) (Six-base) MAPSORT of: pFN21AE5229.seq Check: 1164 from: 1 to: 5263
pFN21AE5229 5263 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3073 278
Size: 53 83 186 936 1537 2468
Fragments arranged by size:
2468 1537 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2606 1937
Size: 669 4594
Acc65I G'GTAC_C
Cuts at: 2584 2584
Size: 5263
AclI AA'CG_TT
Cuts at: 1563 3390 3763 1563
Size: 1827 373 3063
Fragments arranged by size:
3063 1827 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3326 4710 4978 1632
Size: 1694 1384 268 1917
Fragments arranged by size:
1917 1694 1384 268
AfeI AGC'GCT
Cuts at: 2366 2366
Size: 5263
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5066
AflIII A'CryG_T
Cuts at: 1203 4162 5139 1203
Size: 2959 977 1327
Fragments arranged by size:
2959 1327 977
AgeI A'CCGG_T
Cuts at: 4094 4094
Size: 5263
AhdI GACnn_n'nnGTC
Cuts at: 3992 3992
Size: 5263
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 2342
Size: 32 5231
AlwNI CAG_nnn'CTG
Cuts at: 3006 4578 3006
Size: 1572 3691
ApaLI G'TGCA_C
Cuts at: 3320 4476 3320
Size: 1156 4107
ApoI r'AATT_y
Cuts at: 1493 2572 2798 2950 5059 1493
Size: 1079 226 152 2109 1697
Fragments arranged by size:
2109 1697 1079 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3817 160
Size: 872 2785 1606
Fragments arranged by size:
2785 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5263
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 2588 1870
Size: 73 118 527 4545
Fragments arranged by size:
4545 527 118 73
BamHI G'GATC_C
Cuts at: 2232 2593 2960 2232
Size: 361 367 4535
Fragments arranged by size:
4535 367 361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2584 4039 618
Size: 325 217 627 363 434 1455 1842
Fragments arranged by size:
1842 1455 627 434 363 325 217
BanII G_rGCy'C
Cuts at: 1391 2403 2582 1391
Size: 1012 179 4072
Fragments arranged by size:
4072 1012 179
BbeI G_GCGC'C
Cuts at: 2154 2154
Size: 5263
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4292
BbvCI CC'TCA_GC
Cuts at: 2175 2175
Size: 5263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2324 2358 3454 3488 1903
Size: 34 387 34 1096 34 3678
Fragments arranged by size:
3678 1096 387 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3155 4365 3155
Size: 1210 4053
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5263
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3874 136
Size: 107 122 71 631 605 2202 1525
Fragments arranged by size:
2202 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5258 5258
Size: 5263
BlpI GC'TnA_GC
Cuts at: 2680 2680
Size: 5263
Bme1580I G_kGCm'C
Cuts at: 1790 3324 4480 1790
Size: 1534 1156 2573
Fragments arranged by size:
2573 1534 1156
BmgBI CAC'GTC
Cuts at: 1318 2319 1318
Size: 1001 4262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2994 3952 442
Size: 416 53 83 399 24 1577 958 1753
Fragments arranged by size:
1753 1577 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 3923 1239
Size: 1130 1554 2579
Fragments arranged by size:
2579 1554 1130
Bpu10I CC'TnA_GC
Cuts at: 2175 2175
Size: 5263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 3388 4253 4551 2120
Size: 1268 865 298 2832
Fragments arranged by size:
2832 1268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2071 2420 3926 882
Size: 617 572 349 1506 2219
Fragments arranged by size:
2219 1506 617 572 349
BsaAI yAC'GTr
Cuts at: 493 1204 2377 3054 493
Size: 711 1173 677 2702
Fragments arranged by size:
2702 1173 711 677
BsaBI GATnn'nnATC
Cuts at: 5257 5257
Size: 5263
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2151 3070 3452
Size: 53 83 186 936 516 102 919 382
Cuts at: 3452 4087 275
Size: 635 1451
Fragments arranged by size:
1451 936 919 635 516 382 186 102
83 53
BsaWI w'CCGG_w
Cuts at: 3696 4094 4368 4515 4926 3696
Size: 398 274 147 411 4033
Fragments arranged by size:
4033 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 1178
Size: 30 165 30 939 30 4069
Fragments arranged by size:
4069 939 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5263
BseYI C'CCAG_C
Cuts at: 2122 4466 4962 2122
Size: 2344 496 2423
Fragments arranged by size:
2423 2344 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4690
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2713 3474 4502 1540
Size: 275 186 712 761 1028 2301
Fragments arranged by size:
2301 1028 761 712 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 3324 3409 4480 2403
Size: 921 85 1071 3186
Fragments arranged by size:
3186 1071 921 85
BsmI GAATG_Cn'
Cuts at: 2774 2867 2774
Size: 93 5170
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2403 2582 3324 3409 4480 1391
Size: 399 613 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 613 399 179 85
BspEI T'CCGG_A
Cuts at: 4926 4926
Size: 5263
BspHI T'CATG_A
Cuts at: 3152 4882 3152
Size: 1730 3533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2618 844
Size: 1774 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3150 1394
Size: 429 1327 3507
Fragments arranged by size:
3507 1327 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3758 3932 65
Size: 1183 2510 174 1396
Fragments arranged by size:
2510 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2298 3907 4094 1700
Size: 110 488 1609 187 2869
Fragments arranged by size:
2869 1609 488 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5263
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5263
BssSI C'ACGA_G
Cuts at: 3323 4335 3323
Size: 1012 4251
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5263
BstBI TT'CG_AA
Cuts at: 4067 4067
Size: 5263
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5263
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2287
Size: 5263
BstYI r'GATC_y
Cuts at: 1972 2232 2593 2960 3345 3362 4814 4900 4912
Size: 260 361 367 385 17 1452 86 12
Cuts at: 4912 5258 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 361 346 260 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2328 513
Size: 552 1263 3448
Fragments arranged by size:
3448 1263 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4524
Fragments arranged by size:
4524 683 56
BtsI GCAGTG_nn'
Cuts at: 2779 3573 3593 2779
Size: 794 20 4449
Fragments arranged by size:
4449 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5228
DraI TTT'AAA
Cuts at: 2567 2567
Size: 5263
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2317 4270 809
Size: 507 1001 1953 1802
Fragments arranged by size:
1953 1802 1001 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2710 3599 5228
Size: 54 1425 50 275 18 880 889 1629
Cuts at: 5228 8
Size: 43
Fragments arranged by size:
1629 1425 889 880 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2710 1537
Size: 275 898 4090
Fragments arranged by size:
4090 898 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3193 1353
Size: 1840 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3850 4224 4370 387
Size: 1064 138 2261 374 146 1280
Fragments arranged by size:
2261 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2401 2401
Size: 5263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 3326 3923 4710 4978 1239
Size: 393 737 957 597 787 268 1524
Fragments arranged by size:
1524 957 787 737 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 2279
Size: 5263
EcoO109I rG'GnC_Cy
Cuts at: 2284 2284
Size: 5263
EcoRI G'AATT_C
Cuts at: 2572 2950 2572
Size: 378 4885
FspI TGC'GCA
Cuts at: 3769 3769
Size: 5263
HaeII r_GCGC'y
Cuts at: 1678 2154 2172 2368 4410 1678
Size: 476 18 196 2042 2531
Fragments arranged by size:
2531 2042 476 196 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2256 2288 3904 3936 3978
Size: 32 135 32 684 32 1616 32 42
Cuts at: 3978 4010 1373
Size: 32 2626
Fragments arranged by size:
2626 1616 684 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2607 2853 669
Size: 988 281 669 246 3079
Fragments arranged by size:
3079 988 669 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5263
HpaI GTT'AAC
Cuts at: 2853 2853
Size: 5263
KasI G'GCGC_C
Cuts at: 2150 2150
Size: 5263
KpnI G_GTAC'C
Cuts at: 2588 2588
Size: 5263
MfeI C'AATT_G
Cuts at: 2862 2862
Size: 5263
MluI A'CGCG_T
Cuts at: 5139 5139
Size: 5263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4377 4561 1305
Size: 3072 184 2007
Fragments arranged by size:
3072 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3441
Fragments arranged by size:
3441 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3221 3580 3739 518
Size: 648 2055 359 159 2042
Fragments arranged by size:
2055 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2210 2330 2679 3356 4504 4749 1606
Size: 604 120 349 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 604 349 245 120
NarI GG'CG_CC
Cuts at: 2151 2151
Size: 5263
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4711
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5263
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5263
NotI GC'GGCC_GC
Cuts at: 2710 2710
Size: 5263
NspI r_CATG'y
Cuts at: 1132 1229 2621 4166 1132
Size: 97 1392 1545 2229
Fragments arranged by size:
2229 1545 1392 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5263
PciI A'CATG_T
Cuts at: 4162 4162
Size: 5263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 1108
Size: 1269 3994
PmeI GTTT'AAAC
Cuts at: 2567 2567
Size: 5263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2342 2374 3332 3364 4845 4877 2342
Size: 32 958 32 1481 32 2728
Fragments arranged by size:
2728 1481 958 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5263
PsiI TTA'TAA
Cuts at: 2833 2833
Size: 5263
PstI C_TGCA'G
Cuts at: 830 2615 830
Size: 1785 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5263
SacI G_AGCT'C
Cuts at: 2403 2403
Size: 5263
SacII CC_GC'GG
Cuts at: 2331 2331
Size: 5263
SalI G'TCGA_C
Cuts at: 1936 2605 1936
Size: 669 4594
SbfI CC_TGCA'GG
Cuts at: 2615 2615
Size: 5263
ScaI AGT'ACT
Cuts at: 3511 5223 3511
Size: 1712 3551
SfcI C'TryA_G
Cuts at: 826 1045 2611 3746 4427 4618 826
Size: 219 1566 1135 681 191 1471
Fragments arranged by size:
1566 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2152 2152
Size: 5263
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5263
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5263
SmaI CCC'GGG
Cuts at: 1872 2063 2590 1872
Size: 191 527 4545
Fragments arranged by size:
4545 527 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 3367 4268 4530 820
Size: 197 926 192 1232 901 262 1553
Fragments arranged by size:
1553 1232 926 901 262 197 192
SnaBI TAC'GTA
Cuts at: 493 3054 493
Size: 2561 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5263
SphI G_CATG'C
Cuts at: 1132 2621 1132
Size: 1489 3774
SspI AAT'ATT
Cuts at: 5 52 3187 5
Size: 47 3135 2081
Fragments arranged by size:
3135 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5263
StyI C'CwwG_G
Cuts at: 513 1065 2250 2280 2702 513
Size: 552 1185 30 422 3074
Fragments arranged by size:
3074 1185 552 422 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2411 3290 3307 3460 3645 4090 325
Size: 1255 831 879 17 153 185 445 1498
Fragments arranged by size:
1498 1255 879 831 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3509 5221 96
Size: 274 80 33 51 1443 1532 1712 138
Fragments arranged by size:
1712 1532 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5223 5255 5223
Size: 32 5231
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5263
XbaI T'CTAG_A
Cuts at: 2599 2599
Size: 5263
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5263
XmaI C'CCGG_G
Cuts at: 1870 2061 2588 1870
Size: 191 527 4545
Fragments arranged by size:
4545 527 191
XmnI GAAnn'nnTTC
Cuts at: 3392 3392
Size: 5263
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3071 276
Size: 53 83 186 936 1537 2468
Fragments arranged by size:
2468 1537 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI
SfiI SrfI SwaI XcmI