(Linear) (Six-base) MAPSORT of: KIEE5229.seq Check: 4215 from: 1 to: 558 KIEE5229.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AfeI AGC'GCT Cuts at: 0 361 558 Size: 361 197 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 337 369 558 Size: 337 32 189 Fragments arranged by size: 337 189 32 AvaI C'yCGr_G Cuts at: 0 56 558 Size: 56 502 BamHI G'GATC_C Cuts at: 0 227 558 Size: 227 331 BanI G'GyrC_C Cuts at: 0 145 558 Size: 145 413 BanII G_rGCy'C Cuts at: 0 398 558 Size: 398 160 BbeI G_GCGC'C Cuts at: 0 149 558 Size: 149 409 BbvCI CC'TCA_GC Cuts at: 0 170 558 Size: 170 388 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 319 353 558 Size: 319 34 205 Fragments arranged by size: 319 205 34 BmgBI CAC'GTC Cuts at: 0 314 558 Size: 314 244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 558 Size: 364 194 Bpu10I CC'TnA_GC Cuts at: 0 170 558 Size: 170 388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 558 Size: 115 443 BsaI GGTCTCn'nnnn_ Cuts at: 0 66 415 558 Size: 66 349 143 Fragments arranged by size: 349 143 66 BsaAI yAC'GTr Cuts at: 0 372 558 Size: 372 186 BsaHI Gr'CG_yC Cuts at: 0 44 146 558 Size: 44 102 412 Fragments arranged by size: 412 102 44 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 337 367 558 Size: 337 30 191 Fragments arranged by size: 337 191 30 BseYI C'CCAG_C Cuts at: 0 117 558 Size: 117 441 BsiHKAI G_wGCw'C Cuts at: 0 398 558 Size: 398 160 Bsp1286I G_dGCh'C Cuts at: 0 398 558 Size: 398 160 BsrFI r'CCGG_y Cuts at: 0 293 558 Size: 293 265 BstXI CCAn_nnnn'nTGG Cuts at: 0 282 558 Size: 282 276 BstYI r'GATC_y Cuts at: 0 227 558 Size: 227 331 BtgI C'CryG_G Cuts at: 0 323 558 Size: 323 235 DrdI GACnn_nn'nnGTC Cuts at: 0 312 558 Size: 312 246 EcoICRI GAG'CTC Cuts at: 0 396 558 Size: 396 162 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 558 Size: 364 194 EcoNI CCTnn'n_nnAGG Cuts at: 0 274 558 Size: 274 284 EcoO109I rG'GnC_Cy Cuts at: 0 279 558 Size: 279 279 HaeII r_GCGC'y Cuts at: 0 149 167 363 558 Size: 149 18 196 195 Fragments arranged by size: 196 195 149 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 251 283 558 Size: 251 32 275 Fragments arranged by size: 275 251 32 KasI G'GCGC_C Cuts at: 0 145 558 Size: 145 413 MspA1I CmG'CkG Cuts at: 0 205 325 558 Size: 205 120 233 Fragments arranged by size: 233 205 120 NarI GG'CG_CC Cuts at: 0 146 558 Size: 146 412 PflMI CCAn_nnn'nTGG Cuts at: 0 372 558 Size: 372 186 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 337 369 558 Size: 337 32 189 Fragments arranged by size: 337 189 32 SacI G_AGCT'C Cuts at: 0 398 558 Size: 398 160 SacII CC_GC'GG Cuts at: 0 326 558 Size: 326 232 SfoI GGC'GCC Cuts at: 0 147 558 Size: 147 411 SmaI CCC'GGG Cuts at: 0 58 558 Size: 58 500 SmlI C'TyrA_G Cuts at: 0 130 558 Size: 130 428 StyI C'CwwG_G Cuts at: 0 245 275 558 Size: 245 30 283 Fragments arranged by size: 283 245 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 406 558 Size: 406 152 XmaI C'CCGG_G Cuts at: 0 56 558 Size: 56 502 Enzymes that do cut: AfeI AloI AvaI BamHI BanI BanII BbeI BbvCI BcgI BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsiHKAI Bsp1286I BsrFI BstXI BstYI BtgI DrdI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MspA1I NarI PflMI PpiI SacI SacII SfoI SmaI SmlI StyI TaqII XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BsaBI BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI EarI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI