Restriction map for
pFN21AE5246
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
HphI KasI KpnI KspI MaeI
MaeII MaeIII MfeI MflI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I TspRI Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 448 GAATTC [GA]^AATT[CT]
AlwI 69 GATCC GGATC(4/5)
ApoI 448 GAATTC [GA]^AATT[CT]
Asp700I 463 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 207 TGGCCA TGG^CCA
BanII 329 GAGCTC G[GA]GC[CT]^C
BbvI 433 GCAGC GCAGC(8/12)
BciVI 566 GGATAC GTATCC(6/5)
BcnI 57 CCCGG CC^[GC]GG
BglI 42 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 223 CTGGAG CTGGAG(16/14)
BsaI 226 GAGACC GGTCTC(1/5)
BseRI 529 CTCCTC GAGGAG(10/8)
BsmAI 226 GAGAC GTCTC(1/5)
BsmI 415 GAATGC GAATGC(1/-1)
BsrBI 547 GAGCGG CCGCTC(-3/-3)
BstXI 17 CCAGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 166 CTGAG C^T[ACGT]AG
DrdI 628 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 207 TGGCCA [CT]^GGCC[GA]
EcoNI 438 CCTGCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 448 GAATTC G^AATTC
EcoRV 609 GATATC GAT^ATC
EcoT14I 380 CCAAGG C^C[AT][AT]GG
FauI 113 CCCGC CCCGC(4/6)
HapII 58 CCGG C^CGG
HgaI 441 GCGTC GACGC(5/10)
HpaII 58 CCGG C^CGG
MluNI 207 TGGCCA TGG^CCA
MscI 207 TGGCCA TGG^CCA
MseI 161 TTAA T^TAA
MspI 58 CCGG C^CGG
NciI 57 CCCGG CC^[GC]GG
NdeI 311 CATATG CA^TATG
PflMI 617 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 329 GAGCTC GAGCT^C
SapI 643 GCTCTTC GCTCTTC(1/4)
SfcI 78 CTACAG C^T[GA][CT]AG
StyI 380 CCAAGG C^C[AT][AT]GG
Tru9I 161 TTAA T^TAA
TseI 433 GCAGC G^C[AT]GC
Tsp509I 449 AATT ^AATT
Van91I 617 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 380 CCAAGGAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 463 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 186 GTAC GT^AC
273 GTAC
AspHI 329 GAGCTC G[AT]GC[AT]^C
641 GTGCTC
BmyI 329 GAGCTC G[AGT]GC[ACT]^C
641 GTGCTC
BsaBI 69 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
283 GATGCTTATC
BsiHKAI 329 GAGCTC G[AT]GC[AT]^C
641 GTGCTC
BsiYI 438 CCTGCGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
617 CCATGAAGTGG
BslI 438 CCTGCGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
617 CCATGAAGTGG
Bsp1286I 329 GAGCTC G[AGT]GC[ACT]^C
641 GTGCTC
BsrI 570 ACTGG ACTGG(1/-1)
52 CCAGT
DpnII 69 GATC ^GATC
246 GATC
EarI 644 CTCTTC CTCTTC(1/4)
463 GAAGAG
HincII 154 GTCAAC GT[CT]^[GA]AC
239 GTCAAC
HindII 154 GTCAAC GT[CT]^[GA]AC
239 GTCAAC
Ksp632I 644 CTCTTC CTCTTC(1/4)
463 GAAGAG
MamI 69 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
283 GATGCTTATC
MboI 69 GATC ^GATC
246 GATC
MboII 463 GAAGA GAAGA(8/7)
645 TCTTC
NdeII 69 GATC ^GATC
246 GATC
RsaI 186 GTAC GT^AC
273 GTAC
Sau3AI 69 GATC ^GATC
246 GATC
SmlI 124 CTCAAG C^T[CT][GA]AG
472 CTTGAG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AvaII BsaJI BsmFI
BssKI BstF5I BstNI Cac8I Cfr13I
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MnlI MvaI
MwoI NlaIII NlaIV PpuMI Sau96I
ScrFI SfaNI TfiI