(Circular) (Six-base) MAPSORT of: pFN21AE5246.seq Check: 299 from: 1 to: 5434 pFN21AE5246 5434 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3244 278 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2777 1937 Size: 840 4594 Acc65I G'GTAC_C Cuts at: 2755 2755 Size: 5434 AclI AA'CG_TT Cuts at: 1563 3561 3934 1563 Size: 1998 373 3063 Fragments arranged by size: 3063 1998 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 3497 4881 5149 1632 Size: 821 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1044 821 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5237 AflIII A'CryG_T Cuts at: 1203 4333 5310 1203 Size: 3130 977 1327 Fragments arranged by size: 3130 1327 977 AgeI A'CCGG_T Cuts at: 4265 4265 Size: 5434 AhdI GACnn_n'nnGTC Cuts at: 4163 4163 Size: 5434 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5434 AlwNI CAG_nnn'CTG Cuts at: 3177 4749 3177 Size: 1572 3862 ApaLI G'TGCA_C Cuts at: 3491 4647 3491 Size: 1156 4278 ApoI r'AATT_y Cuts at: 1493 2453 2743 2969 3121 5230 1493 Size: 960 290 226 152 2109 1697 Fragments arranged by size: 2109 1697 960 290 226 152 AseI AT'TA_AT Cuts at: 160 1032 3988 160 Size: 872 2956 1606 Fragments arranged by size: 2956 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 AvaI C'yCGr_G Cuts at: 1870 1943 2759 1870 Size: 73 816 4545 Fragments arranged by size: 4545 816 73 BamHI G'GATC_C Cuts at: 2764 3131 2764 Size: 367 5067 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2755 4210 618 Size: 325 217 627 968 1455 1842 Fragments arranged by size: 1842 1455 968 627 325 217 BanII G_rGCy'C Cuts at: 1391 2338 2753 1391 Size: 947 415 4072 Fragments arranged by size: 4072 947 415 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3625 3659 1903 Size: 34 1688 34 3678 Fragments arranged by size: 3678 1688 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2565 3326 4536 2565 Size: 761 1210 3463 Fragments arranged by size: 3463 1210 761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2053 4045 136 Size: 107 122 71 631 605 381 1992 1525 Fragments arranged by size: 1992 1525 631 605 381 122 107 71 BglII A'GATC_T Cuts at: 5429 5429 Size: 5434 BlpI GC'TnA_GC Cuts at: 2851 2851 Size: 5434 Bme1580I G_kGCm'C Cuts at: 1790 3495 4651 1790 Size: 1705 1156 2573 Fragments arranged by size: 2573 1705 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3165 4123 442 Size: 416 53 83 399 24 1748 958 1753 Fragments arranged by size: 1753 1748 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5434 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 4094 1239 Size: 1010 1845 2579 Fragments arranged by size: 2579 1845 1010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2114 2498 3559 4424 4722 2114 Size: 384 1061 865 298 2826 Fragments arranged by size: 2826 1061 865 384 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2225 4097 882 Size: 617 726 1872 2219 Fragments arranged by size: 2219 1872 726 617 BsaAI yAC'GTr Cuts at: 493 1204 3225 493 Size: 711 2021 2702 Fragments arranged by size: 2702 2021 711 BsaBI GATnn'nnATC Cuts at: 2078 2292 5428 2078 Size: 214 3136 2084 Fragments arranged by size: 3136 2084 214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3241 3623 4258 275 Size: 53 83 186 936 1708 382 635 1451 Fragments arranged by size: 1708 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3867 4265 4539 4686 5097 3867 Size: 398 274 147 411 4204 Fragments arranged by size: 4204 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5209 Fragments arranged by size: 5209 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2525 1186 Size: 1339 4095 BseYI C'CCAG_C Cuts at: 4637 5133 4637 Size: 496 4938 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4861 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2884 3645 4673 1540 Size: 275 186 883 761 1028 2301 Fragments arranged by size: 2301 1028 883 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2338 2650 3495 3580 4651 2338 Size: 312 845 85 1071 3121 Fragments arranged by size: 3121 1071 845 312 85 BsmI GAATG_Cn' Cuts at: 2426 2945 3038 2426 Size: 519 93 4822 Fragments arranged by size: 4822 519 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2338 2650 2753 3495 3580 4651 1391 Size: 399 548 312 103 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 548 399 312 103 85 BspEI T'CCGG_A Cuts at: 5097 5097 Size: 5434 BspHI T'CATG_A Cuts at: 3323 5053 3323 Size: 1730 3704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2789 844 Size: 1945 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2554 3321 1394 Size: 429 731 767 3507 Fragments arranged by size: 3507 767 731 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3929 4103 65 Size: 1183 2681 174 1396 Fragments arranged by size: 2681 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4078 4265 1700 Size: 110 2268 187 2869 Fragments arranged by size: 2869 2268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5434 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5434 BssSI C'ACGA_G Cuts at: 3494 4506 3494 Size: 1012 4422 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5434 BstBI TT'CG_AA Cuts at: 4238 4238 Size: 5434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5434 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2029 Size: 5434 BstYI r'GATC_y Cuts at: 1972 2764 3131 3516 3533 4985 5071 5083 5429 Size: 792 367 385 17 1452 86 12 346 Cuts at: 5429 1972 Size: 1977 Fragments arranged by size: 1977 1452 792 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4882 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4695 Fragments arranged by size: 4695 683 56 BtsI GCAGTG_nn' Cuts at: 2950 3744 3764 2950 Size: 794 20 4620 Fragments arranged by size: 4620 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5434 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2482 2517 529 Size: 35 1918 35 3446 Fragments arranged by size: 3446 1918 35 35 DraI TTT'AAA Cuts at: 2738 2738 Size: 5434 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2639 4441 809 Size: 507 1323 1802 1802 Fragments arranged by size: 1802 1802 1323 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2212 2881 3770 Size: 54 1425 50 275 18 382 669 889 Cuts at: 3770 5399 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 669 382 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2881 1537 Size: 275 1069 4090 Fragments arranged by size: 4090 1069 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2463 2655 3364 1353 Size: 1110 192 709 3423 Fragments arranged by size: 3423 1110 709 192 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4021 4395 4541 387 Size: 1064 138 2432 374 146 1280 Fragments arranged by size: 2432 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2336 2336 Size: 5434 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2453 3497 4094 4881 5149 1239 Size: 393 617 204 1044 597 787 268 1524 Fragments arranged by size: 1524 1044 787 617 597 393 268 204 EcoNI CCTnn'n_nnAGG Cuts at: 2447 2447 Size: 5434 EcoO109I rG'GnC_Cy Cuts at: 2025 2115 2371 2632 2025 Size: 90 256 261 4827 Fragments arranged by size: 4827 261 256 90 EcoRI G'AATT_C Cuts at: 2453 2743 3121 2453 Size: 290 378 4766 Fragments arranged by size: 4766 378 290 EcoRV GAT'ATC Cuts at: 2616 2616 Size: 5434 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2492 2492 Size: 5434 FspI TGC'GCA Cuts at: 3940 3940 Size: 5434 HaeII r_GCGC'y Cuts at: 1678 4581 1678 Size: 2903 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4075 4107 4149 4181 1373 Size: 32 135 32 2503 32 42 32 2626 Fragments arranged by size: 2626 2503 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 2246 2778 3024 669 Size: 988 281 223 85 532 246 3079 Fragments arranged by size: 3079 988 532 281 246 223 85 HindIII A'AGCT_T Cuts at: 748 748 Size: 5434 HpaI GTT'AAC Cuts at: 3024 3024 Size: 5434 KpnI G_GTAC'C Cuts at: 2759 2759 Size: 5434 MfeI C'AATT_G Cuts at: 3033 3033 Size: 5434 MluI A'CGCG_T Cuts at: 5310 5310 Size: 5434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4548 4732 1305 Size: 3243 184 2007 Fragments arranged by size: 3243 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2214 10 Size: 54 1425 343 382 3230 Fragments arranged by size: 3230 1425 382 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3392 3751 3910 518 Size: 648 2226 359 159 2042 Fragments arranged by size: 2226 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2850 3527 4675 4920 1606 Size: 1244 677 1148 245 2120 Fragments arranged by size: 2120 1244 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4882 NdeI CA'TA_TG Cuts at: 387 2317 387 Size: 1930 3504 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5434 NotI GC'GGCC_GC Cuts at: 2881 2881 Size: 5434 NspI r_CATG'y Cuts at: 1132 1229 2792 4337 1132 Size: 97 1563 1545 2229 Fragments arranged by size: 2229 1563 1545 97 PasI CC'CwG_GG Cuts at: 1348 2029 2051 1348 Size: 681 22 4731 Fragments arranged by size: 4731 681 22 PciI A'CATG_T Cuts at: 4333 4333 Size: 5434 PflMI CCAn_nnn'nTGG Cuts at: 1108 2628 1108 Size: 1520 3914 PfoI T'CCnGG_A Cuts at: 2061 2226 2296 2061 Size: 165 70 5199 Fragments arranged by size: 5199 165 70 PmeI GTTT'AAAC Cuts at: 2738 2738 Size: 5434 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3503 3535 5016 5048 3503 Size: 32 1481 32 3889 Fragments arranged by size: 3889 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2025 2115 2371 2632 2025 Size: 90 256 261 4827 Fragments arranged by size: 4827 261 256 90 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5434 PsiI TTA'TAA Cuts at: 3004 3004 Size: 5434 PstI C_TGCA'G Cuts at: 830 2786 830 Size: 1956 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5434 SacI G_AGCT'C Cuts at: 2338 2338 Size: 5434 SalI G'TCGA_C Cuts at: 1936 2776 1936 Size: 840 4594 SanDI GG'GwC_CC Cuts at: 2632 2632 Size: 5434 SapI GCTCTTCn'nnn_ Cuts at: 2655 2655 Size: 5434 SbfI CC_TGCA'GG Cuts at: 2786 2786 Size: 5434 ScaI AGT'ACT Cuts at: 3682 5394 3682 Size: 1712 3722 SfcI C'TryA_G Cuts at: 826 1045 2083 2782 3917 4598 4789 826 Size: 219 1038 699 1135 681 191 1471 Fragments arranged by size: 1471 1135 1038 699 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5434 SmaI CCC'GGG Cuts at: 1872 2761 1872 Size: 889 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 2477 3538 4439 4701 820 Size: 197 926 186 348 1061 901 262 1553 Fragments arranged by size: 1553 1061 926 901 348 262 197 186 SnaBI TAC'GTA Cuts at: 493 3225 493 Size: 2732 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5434 SphI G_CATG'C Cuts at: 1132 2792 1132 Size: 1660 3774 SspI AAT'ATT Cuts at: 5 52 3358 5 Size: 47 3306 2081 Fragments arranged by size: 3306 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5434 StyI C'CwwG_G Cuts at: 513 1065 2385 2873 513 Size: 552 1320 488 3074 Fragments arranged by size: 3074 1320 552 488 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3461 3478 3631 3816 4261 325 Size: 1255 1881 17 153 185 445 1498 Fragments arranged by size: 1881 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3680 5392 96 Size: 274 80 33 51 1443 1703 1712 138 Fragments arranged by size: 1712 1703 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5394 5426 5394 Size: 32 5402 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5434 XbaI T'CTAG_A Cuts at: 2770 2770 Size: 5434 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2392 2392 Size: 5434 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5434 XmaI C'CCGG_G Cuts at: 1870 2759 1870 Size: 889 4545 XmnI GAAnn'nnTTC Cuts at: 2472 3563 2472 Size: 1091 4343 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3242 276 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI