################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MfeI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspBI SspI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 577 GTAGAC GT^[AC][GT]AC AciI 72 CCGC CCGC(-3/-1) AfaI 273 GTAC GT^AC AflIII 112 ACGCGT A^C[GA][CT]GT AocI 700 CCTGAGG CC^T[ACGT]AGG Asp700I 430 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 679 GAGCTC G[AT]GC[AT]^C AvaI 45 CCCGGG C^[CT]CG[GA]G AvaII 54 GGTCC G^G[AT]CC BbvI 610 GCAGC GCAGC(8/12) BglI 42 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 69 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 460 GAGGAG GAGGAG(10/8) BsiHKAI 679 GAGCTC G[AT]GC[AT]^C BsiYI 45 CCCGGGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 45 CCCGGGGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 634 GTCTC GTCTC(1/5) BsmFI 55 GTCCC GGGAC(10/14) BsoBI 45 CCCGGG C^[CT]CG[GA]G BsrDI 556 GCAATG GCAATG(2/0) BsrFI 51 GCCGGT [GA]^CCGG[CT] BssHII 38 GCGCGC G^CGCGC BstXI 17 CCAGGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 624 AGATCC [GA]^GATC[CT] Bsu36I 700 CCTGAGG CC^T[ACGT]AGG Cfr10I 51 GCCGGT [GA]^CCGG[CT] Eco81I 700 CCTGAGG CC^T[ACGT]AGG Fnu4HI 610 GCAGC GC^[ACGT]GC HphI 263 TCACC GGTGA(8/7) ItaI 610 GCAGC GC^[ACGT]GC MaeIII 710 GTGAC ^GT[ACGT]AC MamI 69 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 624 AGATCC [GA]^GATC[CT] MluI 112 ACGCGT A^CGCGT MspA1I 241 CAGCTG C[AC]G^C[GT]G PvuII 241 CAGCTG CAG^CTG RsaI 273 GTAC GT^AC SacI 679 GAGCTC GAGCT^C SfaNI 74 GCATC GCATC(5/9) SfcI 78 CTACAG C^T[GA][CT]AG SmaI 45 CCCGGG CCC^GGG SpeI 649 ACTAGT A^CTAGT StuI 704 AGGCCT AGG^CCT TaqI 67 TCGA T^CGA TfiI 137 GAATC G^A[AT]TC TseI 610 GCAGC G^C[AT]GC Tsp45I 710 GTGAC ^GT[GC]AC TthHB8I 67 TCGA T^CGA XhoII 624 AGATCC [GA]^GATC[CT] XmaCI 45 CCCGGG C^CCGGG XmaI 45 CCCGGG C^CCGGG XmnI 430 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 39 CGCG CG^CG 113 CGCG AcsI 433 GAATTT [GA]^AATT[CT] 445 GAATTT AlwI 69 GATCC GGATC(4/5) 625 GATCC ApoI 433 GAATTT [GA]^AATT[CT] 445 GAATTT BanI 213 GGTGCC G^G[CT][GA]CC 366 GGTGCC BbsI 409 GAAGAC GAAGAC(2/6) 480 GAAGAC BpuAI 409 GAAGAC GAAGAC(2/6) 480 GAAGAC BsrI 662 ACTGG ACTGG(1/-1) 217 CCAGT BstUI 39 CGCG CG^CG 113 CGCG Cac8I 38 GCGCGC GC[ACGT]^[ACGT]GC 331 GCTTGC CfoI 38 GCGC GCG^C 40 GCGC EcoT14I 131 CCAAGG C^C[AT][AT]GG 374 CCTTGG HgaI 111 GACGC GACGC(5/10) 114 GCGTC HhaI 38 GCGC GCG^C 40 GCGC HinP1I 38 GCGC G^CGC 40 GCGC HindIII 329 AAGCTT A^AGCTT 384 AAGCTT MseI 191 TTAA T^TAA 565 TTAA MvnI 39 CGCG CG^CG 113 CGCG PleI 10 GAGTC GAGTC(4/5) 253 GAGTC StyI 131 CCAAGG C^C[AT][AT]GG 374 CCTTGG Tru9I 191 TTAA T^TAA 565 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AluI BanII BcnI BfaI BmyI BsaJI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII FokI HaeIII HapII HinfI HpaII MaeI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI Tsp509I