(Circular) (Six-base) MAPSORT of: pFN21AE2406.seq Check: 3584 from: 1 to: 9196 pFN21AE2406 9196 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2269 3070 2269 Size: 801 8395 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7006 278 Size: 53 83 186 936 5470 2468 Fragments arranged by size: 5470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6539 1937 Size: 4602 4594 Acc65I G'GTAC_C Cuts at: 4376 6517 4376 Size: 2141 7055 AclI AA'CG_TT Cuts at: 1563 7323 7696 1563 Size: 5760 373 3063 Fragments arranged by size: 5760 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2061 3254 3509 4835 4968 5277 7259 8643 Size: 429 1193 255 1326 133 309 1982 1384 Cuts at: 8643 8911 1632 Size: 268 1917 Fragments arranged by size: 1982 1917 1384 1326 1193 429 309 268 255 133 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8999 AflIII A'CryG_T Cuts at: 1203 4974 5415 8095 9072 1203 Size: 3771 441 2680 977 1327 Fragments arranged by size: 3771 2680 1327 977 441 AgeI A'CCGG_T Cuts at: 8027 8027 Size: 9196 AhdI GACnn_n'nnGTC Cuts at: 5608 7925 5608 Size: 2317 6879 AleI CACnn'nnGTG Cuts at: 1166 2996 1166 Size: 1830 7366 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9196 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3017 3049 3017 Size: 32 9164 AlwNI CAG_nnn'CTG Cuts at: 3992 4046 4523 5141 6939 8511 3992 Size: 54 477 618 1798 1572 4677 Fragments arranged by size: 4677 1798 1572 618 477 54 ApaI G_GGCC'C Cuts at: 3789 4037 4995 5004 5324 5463 5688 6203 6374 Size: 248 958 9 320 139 225 515 171 Cuts at: 6374 3789 Size: 6611 Fragments arranged by size: 6611 958 515 320 248 225 171 139 9 ApaLI G'TGCA_C Cuts at: 7253 8409 7253 Size: 1156 8040 ApoI r'AATT_y Cuts at: 1493 2378 2427 2536 5519 6505 6731 6883 8992 Size: 885 49 109 2983 986 226 152 2109 Cuts at: 8992 1493 Size: 1697 Fragments arranged by size: 2983 2109 1697 986 885 226 152 109 49 AseI AT'TA_AT Cuts at: 160 1032 7750 160 Size: 872 6718 1606 Fragments arranged by size: 6718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9196 AvaI C'yCGr_G Cuts at: 1870 1943 4155 4211 4638 5325 5455 5551 5679 Size: 73 2212 56 427 687 130 96 128 Cuts at: 5679 5689 6411 6521 1870 Size: 10 722 110 4545 Fragments arranged by size: 4545 2212 722 687 427 130 128 110 96 73 56 10 BamHI G'GATC_C Cuts at: 5565 6526 6893 5565 Size: 961 367 7868 Fragments arranged by size: 7868 961 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2618 2894 3369 3586 4376 Size: 325 217 627 831 276 475 217 790 Cuts at: 4376 4511 5978 6517 7972 618 Size: 135 1467 539 1455 1842 Fragments arranged by size: 1842 1467 1455 831 790 627 539 475 325 276 217 217 135 BanII G_rGCy'C Cuts at: 1391 2254 2342 2528 2603 2732 3648 3789 4037 Size: 863 88 186 75 129 916 141 248 Cuts at: 4037 4065 4212 4417 4924 4995 5004 5021 5324 Size: 28 147 205 507 71 9 17 303 Cuts at: 5324 5463 5496 5688 5707 6203 6374 6515 1391 Size: 139 33 192 19 496 171 141 4072 Fragments arranged by size: 4072 916 863 507 496 303 248 205 192 186 171 147 141 141 139 129 88 75 71 33 28 19 17 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2764 3165 3242 5265 6241 928 Size: 971 865 401 77 2023 976 3883 Fragments arranged by size: 3883 2023 976 971 865 401 77 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6007 6041 7387 7421 1903 Size: 34 4070 34 1346 34 3678 Fragments arranged by size: 4070 3678 1346 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3788 7088 8298 3788 Size: 3300 1210 4686 Fragments arranged by size: 4686 3300 1210 BclI T'GATC_A Cuts at: 1548 5754 1548 Size: 4206 4990 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3378 3579 3864 Size: 107 122 71 631 605 1706 201 285 Cuts at: 3864 4032 4167 4527 4590 4609 4674 4728 4737 Size: 168 135 360 63 19 65 54 9 Cuts at: 4737 4872 4999 5008 5683 5805 6207 6226 7807 Size: 135 127 9 675 122 402 19 1581 Cuts at: 7807 136 Size: 1525 Fragments arranged by size: 1706 1581 1525 675 631 605 402 360 285 201 168 135 135 127 122 122 107 71 65 63 54 19 19 9 9 BglII A'GATC_T Cuts at: 5087 6112 9191 5087 Size: 1025 3079 5092 Fragments arranged by size: 5092 3079 1025 BlpI GC'TnA_GC Cuts at: 6613 6613 Size: 9196 Bme1580I G_kGCm'C Cuts at: 1790 3374 3789 4037 4516 4995 5004 5324 5463 Size: 1584 415 248 479 479 9 320 139 Cuts at: 5463 5688 6203 6347 6374 7257 8413 1790 Size: 225 515 144 27 883 1156 2573 Fragments arranged by size: 2573 1584 1156 883 515 479 479 415 320 248 225 144 139 27 9 BmgBI CAC'GTC Cuts at: 1318 5290 1318 Size: 3972 5224 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2599 4293 6927 Size: 416 53 83 399 24 1182 1694 2634 Cuts at: 6927 7885 442 Size: 958 1753 Fragments arranged by size: 2634 1753 1694 1182 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9196 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5515 5623 5515 Size: 108 9088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2326 3530 3560 3756 3945 4023 4341 Size: 941 146 1204 30 196 189 78 318 Cuts at: 4341 4401 4491 4503 5637 6129 6333 7856 1239 Size: 60 90 12 1134 492 204 1523 2579 Fragments arranged by size: 2579 1523 1204 1134 941 492 318 204 196 189 146 90 78 60 30 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2483 7321 8186 8484 2483 Size: 4838 865 298 3195 Fragments arranged by size: 4838 3195 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2573 3222 4058 4317 4346 4544 4752 Size: 617 1074 649 836 259 29 198 208 Cuts at: 4752 7859 882 Size: 3107 2219 Fragments arranged by size: 3107 2219 1074 836 649 617 259 208 198 29 BsaAI yAC'GTr Cuts at: 493 1204 4598 4975 6987 493 Size: 711 3394 377 2012 2702 Fragments arranged by size: 3394 2702 2012 711 377 BsaBI GATnn'nnATC Cuts at: 9190 9190 Size: 9196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2795 7003 7385 8020 Size: 53 83 186 936 1262 4208 382 635 Cuts at: 8020 275 Size: 1451 Fragments arranged by size: 4208 1451 1262 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2359 5099 5742 7629 8027 8301 8448 8859 2359 Size: 2740 643 1887 398 274 147 411 2696 Fragments arranged by size: 2740 2696 1887 643 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2329 2359 2639 2669 6463 Size: 30 165 30 926 30 280 30 3794 Cuts at: 6463 6493 1178 Size: 30 3881 Fragments arranged by size: 3881 3794 926 280 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2729 2827 3327 3488 3538 3656 5486 5504 Size: 1543 98 500 161 50 118 1830 18 Cuts at: 5504 5875 5878 5961 1186 Size: 371 3 83 4421 Fragments arranged by size: 4421 1830 1543 500 371 161 118 98 83 50 18 3 BseYI C'CCAG_C Cuts at: 2870 3409 4531 6377 6458 8399 8895 2870 Size: 539 1122 1846 81 1941 496 3171 Fragments arranged by size: 3171 1941 1846 1122 539 496 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2971 3020 3608 5976 5991 1163 Size: 573 1235 49 588 2368 15 4368 Fragments arranged by size: 4368 2368 1235 588 573 49 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2513 6646 7407 8435 1540 Size: 275 186 512 4133 761 1028 2301 Fragments arranged by size: 4133 2301 1028 761 512 275 186 BsiHKAI G_wGCw'C Cuts at: 2342 2603 2732 3648 4417 5496 5942 7257 7342 Size: 261 129 916 769 1079 446 1315 85 Cuts at: 7342 8413 2342 Size: 1071 3125 Fragments arranged by size: 3125 1315 1079 1071 916 769 446 261 129 85 BsmI GAATG_Cn' Cuts at: 4929 5403 6707 6800 4929 Size: 474 1304 93 7325 Fragments arranged by size: 7325 1304 474 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2254 2342 2528 2603 2732 3374 3648 Size: 399 464 88 186 75 129 642 274 Cuts at: 3648 3789 4037 4065 4212 4417 4516 4924 4995 Size: 141 248 28 147 205 99 408 71 Cuts at: 4995 5004 5021 5324 5463 5496 5688 5707 5942 Size: 9 17 303 139 33 192 19 235 Cuts at: 5942 6203 6347 6374 6515 7257 7342 8413 1391 Size: 261 144 27 141 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 642 464 408 399 303 274 261 248 235 205 192 186 147 144 141 141 139 129 99 88 85 75 71 33 28 27 19 17 9 BspEI T'CCGG_A Cuts at: 2359 5099 5742 8859 2359 Size: 2740 643 3117 2696 Fragments arranged by size: 3117 2740 2696 643 BspHI T'CATG_A Cuts at: 3090 7085 8815 3090 Size: 3995 1730 3471 Fragments arranged by size: 3995 3471 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2269 3070 6551 844 Size: 1425 801 3481 3489 Fragments arranged by size: 3489 3481 1425 801 BsrBI CCG'CTC Cuts at: 1394 1823 7083 1394 Size: 429 5260 3507 Fragments arranged by size: 5260 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2122 2637 3459 4233 5581 6085 7691 Size: 1183 874 515 822 774 1348 504 1606 Cuts at: 7691 7865 65 Size: 174 1396 Fragments arranged by size: 1606 1396 1348 1183 874 822 774 515 504 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2154 4526 5627 6493 7840 8027 1700 Size: 110 344 2372 1101 866 1347 187 2869 Fragments arranged by size: 2869 2372 1347 1101 866 344 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9196 BssSI C'ACGA_G Cuts at: 2217 2415 3897 7256 8268 2217 Size: 198 1482 3359 1012 3145 Fragments arranged by size: 3359 3145 1482 1012 198 BstAPI GCAn_nnn'nTGC Cuts at: 794 5717 5978 794 Size: 4923 261 4012 Fragments arranged by size: 4923 4012 261 BstBI TT'CG_AA Cuts at: 8000 8000 Size: 9196 BstEII G'GTnAC_C Cuts at: 1184 4133 6443 1184 Size: 2949 2310 3937 Fragments arranged by size: 3937 2949 2310 BstXI CCAn_nnnn'nTGG Cuts at: 6302 6302 Size: 9196 BstYI r'GATC_y Cuts at: 1972 3841 3920 5087 5565 6112 6526 6893 7278 Size: 1869 79 1167 478 547 414 367 385 Cuts at: 7278 7295 8747 8833 8845 9191 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1869 1452 1167 547 478 414 385 367 346 86 79 17 12 Bsu36I CC'TnA_GG Cuts at: 4362 4822 4362 Size: 460 8736 BtgI C'CryG_G Cuts at: 513 1065 2004 2682 2694 2890 3591 3861 3888 Size: 552 939 678 12 196 701 270 27 Cuts at: 3888 4300 4488 5853 513 Size: 412 188 1365 3856 Fragments arranged by size: 3856 1365 939 701 678 552 412 270 196 188 27 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3021 3121 487 Size: 683 56 1795 100 6562 Fragments arranged by size: 6562 1795 683 100 56 BtsI GCAGTG_nn' Cuts at: 3264 3298 3313 6712 7506 7526 3264 Size: 34 15 3399 794 20 4934 Fragments arranged by size: 4934 3399 794 34 20 15 ClaI AT'CG_AT Cuts at: 1554 4448 1554 Size: 2894 6302 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9161 DraI TTT'AAA Cuts at: 6500 6500 Size: 9196 DraIII CAC_nnn'GTG Cuts at: 3980 5197 3980 Size: 1217 7979 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2884 3819 5544 5664 8203 809 Size: 507 1568 935 1725 120 2539 1802 Fragments arranged by size: 2539 1802 1725 1568 935 507 120 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4384 5035 6643 Size: 54 1425 50 275 18 2554 651 1608 Cuts at: 6643 7532 9161 8 Size: 889 1629 43 Fragments arranged by size: 2554 1629 1608 1425 889 651 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6643 1537 Size: 275 4831 4090 Fragments arranged by size: 4831 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2359 3455 5257 5935 7126 1353 Size: 1006 1096 1802 678 1191 3423 Fragments arranged by size: 3423 1802 1191 1096 1006 678 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2968 3485 5322 6051 6099 6478 Size: 1064 138 1379 517 1837 729 48 379 Cuts at: 6478 7783 8157 8303 387 Size: 1305 374 146 1280 Fragments arranged by size: 1837 1379 1305 1280 1064 729 517 379 374 146 138 48 EcoICRI GAG'CTC Cuts at: 2340 2601 2730 3646 4415 5494 2340 Size: 261 129 916 769 1079 6042 Fragments arranged by size: 6042 1079 916 769 261 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2180 2326 3254 3509 3530 3560 Size: 393 429 119 146 928 255 21 30 Cuts at: 3560 3756 3945 4023 4341 4401 4491 4503 4835 Size: 196 189 78 318 60 90 12 332 Cuts at: 4835 4968 5277 5637 6129 6333 7259 7856 8643 Size: 133 309 360 492 204 926 597 787 Cuts at: 8643 8911 1239 Size: 268 1524 Fragments arranged by size: 1524 928 926 787 597 492 429 393 360 332 318 309 268 255 204 196 189 146 133 119 90 78 60 30 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 2299 4947 2299 Size: 2648 6548 EcoO109I rG'GnC_Cy Cuts at: 2688 2700 3740 3786 3857 3884 3893 3911 3962 Size: 12 1040 46 71 27 9 18 51 Cuts at: 3962 3998 4033 4034 4043 4124 4151 4232 4313 Size: 36 35 1 9 81 27 81 81 Cuts at: 4313 4349 4358 4466 4475 4493 4502 4520 4574 Size: 36 9 108 9 18 9 18 54 Cuts at: 4574 4610 4634 4748 4766 4775 4991 4992 5001 Size: 36 24 114 18 9 216 1 9 Cuts at: 5001 5111 5138 5321 5364 5459 5460 5546 5602 Size: 110 27 183 43 95 1 86 56 Cuts at: 5602 5648 5657 5675 5684 5685 5807 5825 6008 Size: 46 9 18 9 1 122 18 183 Cuts at: 6008 6166 6199 6317 6353 6370 6371 2688 Size: 158 33 118 36 17 1 5513 Fragments arranged by size: 5513 1040 216 183 183 158 122 118 114 110 108 95 86 81 81 81 71 56 54 51 46 46 43 36 36 36 36 35 33 27 27 27 24 18 18 18 18 18 18 17 12 9 9 9 9 9 9 9 9 9 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 2536 5519 6505 6883 2536 Size: 2983 986 378 4849 Fragments arranged by size: 4849 2983 986 378 EcoRV GAT'ATC Cuts at: 3274 3274 Size: 9196 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4097 4577 4097 Size: 480 8716 FspI TGC'GCA Cuts at: 5783 7702 5783 Size: 1919 7277 HaeII r_GCGC'y Cuts at: 1678 3336 3362 8343 1678 Size: 1658 26 4981 2531 Fragments arranged by size: 4981 2531 1658 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4430 4462 5308 5340 5594 Size: 32 135 32 2858 32 846 32 254 Cuts at: 5594 5626 6331 6363 7837 7869 7911 7943 1373 Size: 32 705 32 1474 32 42 32 2626 Fragments arranged by size: 2858 2626 1474 846 705 254 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6540 6786 669 Size: 988 281 4602 246 3079 Fragments arranged by size: 4602 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9196 HpaI GTT'AAC Cuts at: 6786 6786 Size: 9196 KpnI G_GTAC'C Cuts at: 4380 6521 4380 Size: 2141 7055 MfeI C'AATT_G Cuts at: 6795 6795 Size: 9196 MluI A'CGCG_T Cuts at: 9072 9072 Size: 9196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2568 4722 5515 8310 8494 1305 Size: 1263 2154 793 2795 184 2007 Fragments arranged by size: 2795 2154 2007 1263 793 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5037 10 Size: 54 1425 343 3205 4169 Fragments arranged by size: 4169 3205 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 2996 4973 5594 7154 7513 7672 Size: 648 1162 668 1977 621 1560 359 159 Cuts at: 7672 518 Size: 2042 Fragments arranged by size: 2042 1977 1560 1162 668 648 621 359 159 MspA1I CmG'CkG Cuts at: 1606 2190 2684 3105 3393 3593 4014 4535 5281 Size: 584 494 421 288 200 421 521 746 Cuts at: 5281 5723 6612 7289 8437 8682 1606 Size: 442 889 677 1148 245 2120 Fragments arranged by size: 2120 1148 889 746 677 584 521 494 442 421 421 288 245 200 NaeI GCC'GGC Cuts at: 2156 4528 5629 2156 Size: 2372 1101 5723 Fragments arranged by size: 5723 2372 1101 NcoI C'CATG_G Cuts at: 513 1065 2004 2694 2890 3861 3888 4488 513 Size: 552 939 690 196 971 27 600 5221 Fragments arranged by size: 5221 971 939 690 600 552 196 27 NdeI CA'TA_TG Cuts at: 387 2917 387 Size: 2530 6666 NgoMIV G'CCGG_C Cuts at: 2154 4526 5627 2154 Size: 2372 1101 5723 Fragments arranged by size: 5723 2372 1101 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9196 NotI GC'GGCC_GC Cuts at: 6643 6643 Size: 9196 NspI r_CATG'y Cuts at: 1132 1229 2854 2938 5071 5419 6070 6157 6554 Size: 97 1625 84 2133 348 651 87 397 Cuts at: 6554 8099 1132 Size: 1545 2229 Fragments arranged by size: 2229 2133 1625 1545 651 397 348 97 87 84 PasI CC'CwG_GG Cuts at: 1348 3764 3782 4354 4879 4997 5006 5672 5831 Size: 2416 18 572 525 118 9 666 159 Cuts at: 5831 5965 6323 1348 Size: 134 358 4221 Fragments arranged by size: 4221 2416 666 572 525 358 159 134 118 18 9 PciI A'CATG_T Cuts at: 5415 8095 5415 Size: 2680 6516 PflMI CCAn_nnn'nTGG Cuts at: 1108 2466 3583 1108 Size: 1358 1117 6721 Fragments arranged by size: 6721 1358 1117 PfoI T'CCnGG_A Cuts at: 2637 3916 4480 5653 6349 2637 Size: 1279 564 1173 696 5484 Fragments arranged by size: 5484 1279 1173 696 564 PmeI GTTT'AAAC Cuts at: 6500 6500 Size: 9196 PmlI CAC'GTG Cuts at: 4598 4598 Size: 9196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7265 7297 8778 8810 7265 Size: 32 1481 32 7651 Fragments arranged by size: 7651 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2688 2700 3893 3962 3998 4043 4124 4151 4232 Size: 12 1193 69 36 45 81 27 81 Cuts at: 4232 4313 4349 4358 4475 4493 4502 4574 4634 Size: 81 36 9 117 18 9 72 60 Cuts at: 4634 4748 4766 5111 5138 5364 5546 5602 5657 Size: 114 18 345 27 226 182 56 55 Cuts at: 5657 5825 6008 6317 6353 2688 Size: 168 183 309 36 5531 Fragments arranged by size: 5531 1193 345 309 226 183 182 168 117 114 81 81 81 72 69 60 56 55 45 36 36 36 27 27 18 18 12 9 9 PshAI GACnn'nnGTC Cuts at: 1613 4764 5292 6006 1613 Size: 3151 528 714 4803 Fragments arranged by size: 4803 3151 714 528 PsiI TTA'TAA Cuts at: 6766 6766 Size: 9196 PspOMI G'GGCC_C Cuts at: 3785 4033 4991 5000 5320 5459 5684 6199 6370 Size: 248 958 9 320 139 225 515 171 Cuts at: 6370 3785 Size: 6611 Fragments arranged by size: 6611 958 515 320 248 225 171 139 9 PspXI vC'TCGA_Gb Cuts at: 4155 5679 6411 4155 Size: 1524 732 6940 Fragments arranged by size: 6940 1524 732 PstI C_TGCA'G Cuts at: 830 2445 3271 3392 5280 5998 6548 830 Size: 1615 826 121 1888 718 550 3478 Fragments arranged by size: 3478 1888 1615 826 718 550 121 PvuI CG_AT'CG Cuts at: 2001 2513 2001 Size: 512 8684 PvuII CAG'CTG Cuts at: 3393 4535 5723 3393 Size: 1142 1188 6866 Fragments arranged by size: 6866 1188 1142 SacI G_AGCT'C Cuts at: 2342 2603 2732 3648 4417 5496 2342 Size: 261 129 916 769 1079 6042 Fragments arranged by size: 6042 1079 916 769 261 129 SacII CC_GC'GG Cuts at: 2685 3594 2685 Size: 909 8287 SalI G'TCGA_C Cuts at: 1936 6538 1936 Size: 4602 4594 SanDI GG'GwC_CC Cuts at: 3998 4493 5111 5364 5825 6008 3998 Size: 495 618 253 461 183 7186 Fragments arranged by size: 7186 618 495 461 253 183 SapI GCTCTTCn'nnn_ Cuts at: 3455 5935 3455 Size: 2480 6716 SbfI CC_TGCA'GG Cuts at: 6548 6548 Size: 9196 ScaI AGT'ACT Cuts at: 5312 7444 9156 5312 Size: 2132 1712 5352 Fragments arranged by size: 5352 2132 1712 SexAI A'CCwGG_T Cuts at: 3958 3976 5125 6385 3958 Size: 18 1149 1260 6769 Fragments arranged by size: 6769 1260 1149 18 SfcI C'TryA_G Cuts at: 826 1045 2441 3267 3283 3388 4047 5276 5923 Size: 219 1396 826 16 105 659 1229 647 Cuts at: 5923 5994 6259 6544 7679 8360 8551 826 Size: 71 265 285 1135 681 191 1471 Fragments arranged by size: 1471 1396 1229 1135 826 681 659 647 285 265 219 191 105 71 16 SfiI GGCCn_nnn'nGGCC Cuts at: 3864 4032 4527 4590 4609 4674 4728 4737 4872 Size: 168 495 63 19 65 54 9 135 Cuts at: 4872 4999 5008 5683 5805 6207 6226 3864 Size: 127 9 675 122 402 19 6834 Fragments arranged by size: 6834 675 495 402 168 135 127 122 65 63 54 19 19 9 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9196 SmaI CCC'GGG Cuts at: 1872 4213 5457 5553 5691 6523 1872 Size: 2341 1244 96 138 832 4545 Fragments arranged by size: 4545 2341 1244 832 138 96 SmlI C'TyrA_G Cuts at: 820 1017 1943 2498 4155 5679 6411 7300 8201 Size: 197 926 555 1657 1524 732 889 901 Cuts at: 8201 8463 820 Size: 262 1553 Fragments arranged by size: 1657 1553 1524 926 901 889 732 555 262 197 SnaBI TAC'GTA Cuts at: 493 6987 493 Size: 6494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9196 SphI G_CATG'C Cuts at: 1132 2854 6157 6554 1132 Size: 1722 3303 397 3774 Fragments arranged by size: 3774 3303 1722 397 SspI AAT'ATT Cuts at: 5 52 7120 5 Size: 47 7068 2081 Fragments arranged by size: 7068 2081 47 StuI AGG'CCT Cuts at: 1517 3207 4161 4803 1517 Size: 1690 954 642 5910 Fragments arranged by size: 5910 1690 954 642 StyI C'CwwG_G Cuts at: 513 1065 2004 2694 2890 3096 3347 3789 3861 Size: 552 939 690 196 206 251 442 72 Cuts at: 3861 3888 4057 4255 4488 4752 4762 4789 6635 Size: 27 169 198 233 264 10 27 1846 Cuts at: 6635 513 Size: 3074 Fragments arranged by size: 3074 1846 939 690 552 442 264 251 233 206 198 196 169 72 27 27 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2821 3813 5957 7223 7240 7393 7578 Size: 1255 1241 992 2144 1266 17 153 185 Cuts at: 7578 8023 325 Size: 445 1498 Fragments arranged by size: 2144 1498 1266 1255 1241 992 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5052 5064 5310 Size: 274 80 33 51 1443 3075 12 246 Cuts at: 5310 7442 9154 96 Size: 2132 1712 138 Fragments arranged by size: 3075 2132 1712 1443 274 246 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2329 2361 2541 2573 3966 3998 5550 5582 9156 Size: 32 180 32 1393 32 1552 32 3574 Cuts at: 9156 9188 2329 Size: 32 2337 Fragments arranged by size: 3574 2337 1552 1393 180 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2406 3241 6449 1316 Size: 1090 835 3208 4063 Fragments arranged by size: 4063 3208 1090 835 XbaI T'CTAG_A Cuts at: 6532 6532 Size: 9196 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2463 3073 3363 5577 5593 6455 2463 Size: 610 290 2214 16 862 5204 Fragments arranged by size: 5204 2214 862 610 290 16 XhoI C'TCGA_G Cuts at: 1943 4155 5679 6411 1943 Size: 2212 1524 732 4728 Fragments arranged by size: 4728 2212 1524 732 XmaI C'CCGG_G Cuts at: 1870 4211 5455 5551 5689 6521 1870 Size: 2341 1244 96 138 832 4545 Fragments arranged by size: 4545 2341 1244 832 138 96 XmnI GAAnn'nnTTC Cuts at: 7325 7325 Size: 9196 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7004 276 Size: 53 83 186 936 5470 2468 Fragments arranged by size: 5470 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SfoI SrfI SwaI