Restriction map for
pFN21AE2686
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AcyI AflII
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AvaI AviII AvrII BamHI BbeI
BbrPI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 312 CGCG CG^CG
AccIII 761 TCCGGA T^CCGGA
AclI 1126 AACGTT AA^CGTT
AcsI 757 AAATTC [GA]^AATT[CT]
AhdI 307 GACATCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 418 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 757 AAATTC [GA]^AATT[CT]
Asp700I 663 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 307 GACATCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 646 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 511 GTCTTC GAAGAC(2/6)
BbvCI 1510 GCTGAGG CCTCAGC(-5/-2)
BcgI 363 GCAGCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 511 GTCTTC GAAGAC(2/6)
BsaBI 409 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 913 GAGACC GGTCTC(1/5)
BsaWI 761 TCCGGA [AT]^CCGG[AT]
BseAI 761 TCCGGA T^CCGGA
BsgI 955 CTGCAC GTGCAG(16/14)
BsmBI 1045 CGTCTC CGTCTC(1/5)
BspEI 761 TCCGGA T^CCGGA
BsrDI 827 GCAATG GCAATG(2/0)
BsrI 359 ACTGG ACTGG(1/-1)
BssSI 10 CTCGTG CACGAG(-5/-1)
BstEII 522 GGTAACC G^GT[ACGT]ACC
BstPI 522 GGTAACC G^GT[ACGT]ACC
BstUI 312 CGCG CG^CG
BstYI 1334 AGATCC [GA]^GATC[CT]
DsaI 1094 CCGTGG C^C[GA][CT]GG
Eam1105I 307 GACATCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1354 CTCTTC CTCTTC(1/4)
EcoO65I 522 GGTAACC G^GT[ACGT]ACC
EcoT22I 145 ATGCAT ATGCA^T
HaeII 1191 GGCGCT [GA]GCGC^[CT]
HgaI 313 GCGTC GACGC(5/10)
HincII 1129 GTTGAC GT[CT]^[GA]AC
HindII 1129 GTTGAC GT[CT]^[GA]AC
Ksp632I 1354 CTCTTC CTCTTC(1/4)
MaeII 1127 ACGT A^CGT
MamI 409 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1334 AGATCC [GA]^GATC[CT]
MroI 761 TCCGGA T^CCGGA
MvnI 312 CGCG CG^CG
NdeI 142 CATATG CA^TATG
NsiI 145 ATGCAT ATGCA^T
NspI 782 ACATGC [GA]CATG^[CT]
PflFI 646 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1126 AACGTT AA^CGTT
SapI 1353 GCTCTTC GCTCTTC(1/4)
StuI 1490 AGGCCT AGG^CCT
TfiI 1254 GAATC G^A[AT]TC
Tth111I 646 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 303 TCTAGA T^CTAGA
XhoII 1334 AGATCC [GA]^GATC[CT]
XmnI 663 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 78 GTAC GT^AC
452 GTAC
AlwI 1335 GATCC GGATC(4/5)
1227 GGATC
AocI 964 CCTCAGG CC^T[ACGT]AGG
1086 CCTCAGG
AspHI 7 GAGCTC G[AT]GC[AT]^C
1351 GAGCTC
BanII 7 GAGCTC G[GA]GC[CT]^C
1351 GAGCTC
BseRI 1048 CTCCTC GAGGAG(10/8)
988 GAGGAG
BsiHKAI 7 GAGCTC G[AT]GC[AT]^C
1351 GAGCTC
BsmAI 913 GAGAC GTCTC(1/5)
1046 GTCTC
BstXI 779 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
920 CCACCTCTGTGG
Bsu36I 964 CCTCAGG CC^T[ACGT]AGG
1086 CCTCAGG
Eco81I 964 CCTCAGG CC^T[ACGT]AGG
1086 CCTCAGG
EcoNI 399 CCTGTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1053 CCTGCTGGAGG
EcoT14I 245 CCAAGG C^C[AT][AT]GG
1439 CCAAGG
FauI 1343 CCCGC CCCGC(4/6)
1261 GCGGG
HapII 762 CCGG C^CGG
1225 CCGG
HpaII 762 CCGG C^CGG
1225 CCGG
MspI 762 CCGG C^CGG
1225 CCGG
PstI 166 CTGCAG CTGCA^G
1378 CTGCAG
RsaI 78 GTAC GT^AC
452 GTAC
SacI 7 GAGCTC GAGCT^C
1351 GAGCTC
StyI 245 CCAAGG C^C[AT][AT]GG
1439 CCAAGG
Tsp45I 767 GTGAC ^GT[GC]AC
1034 GTGAC
XcmI 674 CCAACAAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
917 CCACCACCTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AvaII BalI BanI
BbvI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I