(Circular) (Six-base) MAPSORT of: pFN21AE2686.seq Check: 4285 from: 1 to: 6229 pFN21AE2686 6229 bp With 182 enzymes: SgfI * May 11, 2017 14:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4039 278 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 3132 4356 4729 1563 Size: 1569 1224 373 3063 Fragments arranged by size: 3063 1569 1224 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4292 5676 5944 1632 Size: 2660 1384 268 1917 Fragments arranged by size: 2660 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 5128 6105 1203 Size: 3925 977 1327 Fragments arranged by size: 3925 1327 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 2317 4958 2317 Size: 2641 3588 AleI CACnn'nnGTG Cuts at: 1166 2930 1166 Size: 1764 4465 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6229 AlwNI CAG_nnn'CTG Cuts at: 2428 3972 5544 2428 Size: 1544 1572 3113 Fragments arranged by size: 3113 1572 1544 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 2762 3538 3764 3916 6025 1493 Size: 1269 776 226 152 2109 1697 Fragments arranged by size: 2109 1697 1269 776 226 152 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 3554 1870 Size: 73 1611 4545 Fragments arranged by size: 4545 1611 73 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2067 2075 2210 3222 3403 Size: 325 217 627 280 8 135 1012 181 Cuts at: 3403 3550 5005 618 Size: 147 1455 1842 Fragments arranged by size: 1842 1455 1012 627 325 280 217 181 147 135 8 BanII G_rGCy'C Cuts at: 1391 2016 3360 3548 1391 Size: 625 1344 188 4072 Fragments arranged by size: 4072 1344 625 188 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2509 928 Size: 971 610 4648 Fragments arranged by size: 4648 971 610 BbvCI CC'TCA_GC Cuts at: 3516 3516 Size: 6229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2357 2391 4420 4454 1903 Size: 34 420 34 2029 34 3678 Fragments arranged by size: 3678 2029 420 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BfrBI ATG'CAT Cuts at: 2152 2152 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4840 136 Size: 107 122 71 631 605 3168 1525 Fragments arranged by size: 3168 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6224 6224 Size: 6229 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 2072 2213 3173 4290 5446 1790 Size: 282 141 960 1117 1156 2573 Fragments arranged by size: 2573 1156 1117 960 282 141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3960 4918 442 Size: 416 53 83 399 24 2543 958 1753 Fragments arranged by size: 2543 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 3083 3124 3419 3520 4889 1239 Size: 1211 633 41 295 101 1369 2579 Fragments arranged by size: 2579 1369 1211 633 295 101 41 Bpu10I CC'TnA_GC Cuts at: 2630 3246 3516 2630 Size: 616 270 5343 Fragments arranged by size: 5343 616 270 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4354 5219 5517 4354 Size: 865 298 5066 Fragments arranged by size: 5066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 4892 882 Size: 617 1413 1980 2219 Fragments arranged by size: 2219 1980 1413 617 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 2418 6223 2418 Size: 3805 2424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4036 4418 5053 275 Size: 53 83 186 936 2503 382 635 1451 Fragments arranged by size: 2503 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2766 4662 5060 5334 5481 5892 2766 Size: 1896 398 274 147 411 3103 Fragments arranged by size: 3103 1896 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3122 3152 1178 Size: 30 165 30 1719 30 4255 Fragments arranged by size: 4255 1719 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3008 3044 1186 Size: 1822 36 4371 Fragments arranged by size: 4371 1822 36 BseYI C'CCAG_C Cuts at: 3273 5432 5928 3273 Size: 2159 496 3574 Fragments arranged by size: 3574 2159 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2945 1163 Size: 573 1209 4447 Fragments arranged by size: 4447 1209 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3679 4440 5468 1540 Size: 275 186 1678 761 1028 2301 Fragments arranged by size: 2301 1678 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 3360 4290 4375 5446 2016 Size: 1344 930 85 1071 2799 Fragments arranged by size: 2799 1344 1071 930 85 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 BsmBI CGTCTCn'nnnn_ Cuts at: 3056 3056 Size: 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2072 2213 3173 3360 3548 4290 Size: 399 226 56 141 960 187 188 742 Cuts at: 4290 4375 5446 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 960 742 399 226 188 187 141 85 56 BspEI T'CCGG_A Cuts at: 2766 5892 2766 Size: 3126 3103 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3584 844 Size: 2740 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4116 1394 Size: 429 2293 3507 Fragments arranged by size: 3507 2293 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2839 4724 4898 65 Size: 1183 1591 1885 174 1396 Fragments arranged by size: 1885 1591 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4873 5060 1700 Size: 110 3063 187 2869 Fragments arranged by size: 3063 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 2015 4289 5301 2015 Size: 2274 1012 2943 Fragments arranged by size: 2943 2274 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 2527 1184 Size: 1343 4886 BstXI CCAn_nnnn'nTGG Cuts at: 2791 2932 2791 Size: 141 6088 BstYI r'GATC_y Cuts at: 1972 3339 3559 3926 4311 4328 5780 5866 5878 Size: 1367 220 367 385 17 1452 86 12 Cuts at: 5878 6224 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1367 385 367 346 220 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2970 3092 2970 Size: 122 6107 BtgI C'CryG_G Cuts at: 513 1065 3099 513 Size: 552 2034 3643 Fragments arranged by size: 3643 2034 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2299 2402 487 Size: 683 56 1073 103 4314 Fragments arranged by size: 4314 1073 683 103 56 BtsI GCAGTG_nn' Cuts at: 3745 4539 4559 3745 Size: 794 20 5415 Fragments arranged by size: 5415 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2233 2247 Size: 54 1425 50 275 18 313 90 14 Cuts at: 2247 2391 2475 3676 4565 6194 8 Size: 144 84 1201 889 1629 43 Fragments arranged by size: 1629 1425 1201 889 313 275 144 90 84 54 50 43 18 14 EagI C'GGCC_G Cuts at: 1537 1812 3676 1537 Size: 275 1864 4090 Fragments arranged by size: 4090 1864 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3365 4159 1353 Size: 2012 794 3423 Fragments arranged by size: 3423 2012 794 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2014 3358 2014 Size: 1344 4885 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 3083 3124 3419 3520 4292 4889 Size: 393 818 633 41 295 101 772 597 Cuts at: 4889 5676 5944 1239 Size: 787 268 1524 Fragments arranged by size: 1524 818 787 772 633 597 393 295 268 101 41 EcoNI CCTnn'n_nnAGG Cuts at: 2408 3062 2408 Size: 654 5575 EcoO109I rG'GnC_Cy Cuts at: 2578 3270 3317 2578 Size: 692 47 5490 Fragments arranged by size: 5490 692 47 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3469 3469 Size: 6229 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 3200 5376 1678 Size: 1522 2176 2531 Fragments arranged by size: 2531 2176 1522 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 2428 2460 2837 Size: 32 135 32 731 32 93 32 377 Cuts at: 2837 2869 4870 4902 4944 4976 1373 Size: 32 2001 32 42 32 2626 Fragments arranged by size: 2626 2001 731 377 135 93 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3136 3573 3819 669 Size: 988 281 1198 437 246 3079 Fragments arranged by size: 3079 1198 988 437 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2346 5343 5527 1305 Size: 1041 2997 184 2007 Fragments arranged by size: 2997 2007 1041 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 2393 2477 10 Size: 54 1425 343 417 144 84 3762 Fragments arranged by size: 3762 1425 417 343 144 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2786 2801 2930 3207 4187 4546 4705 Size: 648 1620 15 129 277 980 359 159 Cuts at: 4705 518 Size: 2042 Fragments arranged by size: 2042 1620 980 648 359 277 159 129 15 MspA1I CmG'CkG Cuts at: 1606 2245 2371 3266 3421 3645 4322 5470 5715 Size: 639 126 895 155 224 677 1148 245 Cuts at: 5715 1606 Size: 2120 Fragments arranged by size: 2120 1148 895 677 639 245 224 155 126 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5677 NdeI CA'TA_TG Cuts at: 387 2148 387 Size: 1761 4468 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NsiI A_TGCA'T Cuts at: 2154 2154 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 2791 3587 5132 1132 Size: 97 1562 796 1545 2229 Fragments arranged by size: 2229 1562 1545 796 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 3010 3413 1108 Size: 1137 765 403 3924 Fragments arranged by size: 3924 1137 765 403 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2578 3270 3317 2578 Size: 692 47 5490 Fragments arranged by size: 5490 692 47 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PstI C_TGCA'G Cuts at: 830 2175 3387 3581 830 Size: 1345 1212 194 3478 Fragments arranged by size: 3478 1345 1212 194 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2245 2371 3421 2245 Size: 126 1050 5053 Fragments arranged by size: 5053 1050 126 SacI G_AGCT'C Cuts at: 2016 3360 2016 Size: 1344 4885 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SanDI GG'GwC_CC Cuts at: 2578 3270 2578 Size: 692 5537 SapI GCTCTTCn'nnn_ Cuts at: 3365 3365 Size: 6229 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SfcI C'TryA_G Cuts at: 826 1045 2171 2220 3383 3577 4712 5393 5584 Size: 219 1126 49 1163 194 1135 681 191 Cuts at: 5584 826 Size: 1471 Fragments arranged by size: 1471 1163 1135 1126 681 219 194 191 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 3556 1872 Size: 1684 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4333 5234 5496 820 Size: 197 926 2390 901 262 1553 Fragments arranged by size: 2390 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 3497 1517 Size: 1980 4249 StyI C'CwwG_G Cuts at: 513 1065 2250 3444 3668 513 Size: 552 1185 1194 224 3074 Fragments arranged by size: 3074 1194 1185 552 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2495 4256 4273 4426 4611 5056 325 Size: 1255 915 1761 17 153 185 445 1498 Fragments arranged by size: 1761 1498 1255 915 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2456 4475 6187 Size: 274 80 33 51 1443 479 2019 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 2019 1712 1443 479 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6189 6221 6189 Size: 32 6197 Tth111I GACn'n_nGTC Cuts at: 1316 2654 1316 Size: 1338 4891 XbaI T'CTAG_A Cuts at: 2308 3565 2308 Size: 1257 4972 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2686 2929 2686 Size: 243 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 3554 1870 Size: 1684 4545 XmnI GAAnn'nnTTC Cuts at: 2672 4358 2672 Size: 1686 4543 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4037 276 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI