(Linear) (Six-base) MAPSORT of: KIEE2686.seq Check: 5495 from: 1 to: 1524 KIEE2686.orf With 182 enzymes: SgfI * May 11, 2017 11:17 .. AclI AA'CG_TT Cuts at: 0 1127 1524 Size: 1127 397 AhdI GACnn_n'nnGTC Cuts at: 0 312 1524 Size: 312 1212 AleI CACnn'nnGTG Cuts at: 0 925 1524 Size: 925 599 AlwNI CAG_nnn'CTG Cuts at: 0 423 1524 Size: 423 1101 ApoI r'AATT_y Cuts at: 0 757 1524 Size: 757 767 BanI G'GyrC_C Cuts at: 0 62 70 205 1217 1398 1524 Size: 62 8 135 1012 181 126 Fragments arranged by size: 1012 181 135 126 62 8 BanII G_rGCy'C Cuts at: 0 11 1355 1524 Size: 11 1344 169 Fragments arranged by size: 1344 169 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 504 1524 Size: 504 1020 BbvCI CC'TCA_GC Cuts at: 0 1511 1524 Size: 1511 13 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 352 386 1524 Size: 352 34 1138 Fragments arranged by size: 1138 352 34 BfrBI ATG'CAT Cuts at: 0 147 1524 Size: 147 1377 Bme1580I G_kGCm'C Cuts at: 0 67 208 1168 1524 Size: 67 141 960 356 Fragments arranged by size: 960 356 141 67 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 1078 1119 1414 1515 1524 Size: 445 633 41 295 101 9 Fragments arranged by size: 633 445 295 101 41 9 Bpu10I CC'TnA_GC Cuts at: 0 625 1241 1511 1524 Size: 625 616 270 13 Fragments arranged by size: 625 616 270 13 BsaI GGTCTCn'nnnn_ Cuts at: 0 907 1524 Size: 907 617 BsaBI GATnn'nnATC Cuts at: 0 413 1524 Size: 413 1111 BsaWI w'CCGG_w Cuts at: 0 761 1524 Size: 761 763 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1117 1147 1524 Size: 1117 30 377 Fragments arranged by size: 1117 377 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1003 1039 1524 Size: 1003 36 485 Fragments arranged by size: 1003 485 36 BseYI C'CCAG_C Cuts at: 0 1268 1524 Size: 1268 256 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 940 1524 Size: 940 584 BsiHKAI G_wGCw'C Cuts at: 0 11 1355 1524 Size: 11 1344 169 Fragments arranged by size: 1344 169 11 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1051 1524 Size: 1051 473 Bsp1286I G_dGCh'C Cuts at: 0 11 67 208 1168 1355 1524 Size: 11 56 141 960 187 169 Fragments arranged by size: 960 187 169 141 56 11 BspEI T'CCGG_A Cuts at: 0 761 1524 Size: 761 763 BsrDI GCAATG_nn' Cuts at: 0 834 1524 Size: 834 690 BssSI C'ACGA_G Cuts at: 0 10 1524 Size: 10 1514 BstEII G'GTnAC_C Cuts at: 0 522 1524 Size: 522 1002 BstXI CCAn_nnnn'nTGG Cuts at: 0 786 927 1524 Size: 786 141 597 Fragments arranged by size: 786 597 141 BstYI r'GATC_y Cuts at: 0 1334 1524 Size: 1334 190 Bsu36I CC'TnA_GG Cuts at: 0 965 1087 1524 Size: 965 122 437 Fragments arranged by size: 965 437 122 BtgI C'CryG_G Cuts at: 0 1094 1524 Size: 1094 430 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 294 397 1524 Size: 294 103 1127 Fragments arranged by size: 1127 294 103 EaeI y'GGCC_r Cuts at: 0 138 228 242 386 470 1524 Size: 138 90 14 144 84 1054 Fragments arranged by size: 1054 144 138 90 84 14 EarI CTCTTCn'nnn_ Cuts at: 0 1360 1524 Size: 1360 164 EcoICRI GAG'CTC Cuts at: 0 9 1353 1524 Size: 9 1344 171 Fragments arranged by size: 1344 171 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 1078 1119 1414 1515 1524 Size: 445 633 41 295 101 9 Fragments arranged by size: 633 445 295 101 41 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 403 1057 1524 Size: 403 654 467 Fragments arranged by size: 654 467 403 EcoO109I rG'GnC_Cy Cuts at: 0 573 1265 1312 1524 Size: 573 692 47 212 Fragments arranged by size: 692 573 212 47 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1464 1524 Size: 1464 60 HaeII r_GCGC'y Cuts at: 0 1195 1524 Size: 1195 329 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 298 330 423 455 832 864 1524 Size: 298 32 93 32 377 32 660 Fragments arranged by size: 660 377 298 93 32 32 32 HincII GTy'rAC Cuts at: 0 1131 1524 Size: 1131 393 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 341 1524 Size: 341 1183 MscI TGG'CCA Cuts at: 0 244 388 472 1524 Size: 244 144 84 1052 Fragments arranged by size: 1052 244 144 84 MslI CAynn'nnrTG Cuts at: 0 781 796 925 1202 1524 Size: 781 15 129 277 322 Fragments arranged by size: 781 322 277 129 15 MspA1I CmG'CkG Cuts at: 0 240 366 1261 1416 1524 Size: 240 126 895 155 108 Fragments arranged by size: 895 240 155 126 108 NdeI CA'TA_TG Cuts at: 0 143 1524 Size: 143 1381 NsiI A_TGCA'T Cuts at: 0 149 1524 Size: 149 1375 NspI r_CATG'y Cuts at: 0 786 1524 Size: 786 738 PflMI CCAn_nnn'nTGG Cuts at: 0 240 1005 1408 1524 Size: 240 765 403 116 Fragments arranged by size: 765 403 240 116 PpuMI rG'GwC_Cy Cuts at: 0 573 1265 1312 1524 Size: 573 692 47 212 Fragments arranged by size: 692 573 212 47 PstI C_TGCA'G Cuts at: 0 170 1382 1524 Size: 170 1212 142 Fragments arranged by size: 1212 170 142 PvuII CAG'CTG Cuts at: 0 240 366 1416 1524 Size: 240 126 1050 108 Fragments arranged by size: 1050 240 126 108 SacI G_AGCT'C Cuts at: 0 11 1355 1524 Size: 11 1344 169 Fragments arranged by size: 1344 169 11 SanDI GG'GwC_CC Cuts at: 0 573 1265 1524 Size: 573 692 259 Fragments arranged by size: 692 573 259 SapI GCTCTTCn'nnn_ Cuts at: 0 1360 1524 Size: 1360 164 SfcI C'TryA_G Cuts at: 0 166 215 1378 1524 Size: 166 49 1163 146 Fragments arranged by size: 1163 166 146 49 StuI AGG'CCT Cuts at: 0 1492 1524 Size: 1492 32 StyI C'CwwG_G Cuts at: 0 245 1439 1524 Size: 245 1194 85 Fragments arranged by size: 1194 245 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 490 1524 Size: 490 1034 TatI w'GTAC_w Cuts at: 0 451 1524 Size: 451 1073 Tth111I GACn'n_nGTC Cuts at: 0 649 1524 Size: 649 875 XbaI T'CTAG_A Cuts at: 0 303 1524 Size: 303 1221 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 681 924 1524 Size: 681 243 600 Fragments arranged by size: 681 600 243 XmnI GAAnn'nnTTC Cuts at: 0 667 1524 Size: 667 857 Enzymes that do cut: AclI AhdI AleI AlwNI ApoI BanI BanII BbsI BbvCI BcgI BfrBI Bme1580I BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BsrDI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NdeI NsiI NspI PflMI PpuMI PstI PvuII SacI SanDI SapI SfcI StuI StyI TaqII TatI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AcuI AfeI AflII AflIII AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI BsaHI BsiEI BsiWI BsmI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI XhoI XmaI ZraI