################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsiWI BsmBI Bsp1407I BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstZ17I CelII CpoI DraI DrdI Eam1105I EcoNI EcoRI EcoRV FbaI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MfeI MluI MunI NarI NdeI NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 145 GTAGAC GT^[AC][GT]AC AccIII 878 TCCGGA T^CCGGA AocI 381 CCTGAGG CC^T[ACGT]AGG Aor51HI 296 AGCGCT AGC^GCT BamHI 249 GGATCC G^GATCC BbvCI 1154 CCTCAGC CCTCAGC(-5/-2) BciVI 1279 GGATAC GTATCC(6/5) BsaBI 421 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 878 TCCGGA [AT]^CCGG[AT] BseAI 878 TCCGGA T^CCGGA BseRI 1336 CTCCTC GAGGAG(10/8) BsgI 1449 CTGCAC GTGCAG(16/14) BsmI 1223 GAATGC GAATGC(1/-1) BspDI 1514 ATCGAT AT^CGAT BspEI 878 TCCGGA T^CCGGA BsrDI 1003 CATTGC GCAATG(2/0) BsrFI 1693 GCCGGC [GA]^CCGG[CT] BssSI 1291 CTCGTG CACGAG(-5/-1) BstBI 455 TTCGAA TT^CGAA BstYI 249 GGATCC [GA]^GATC[CT] Bsu36I 381 CCTGAGG CC^T[ACGT]AGG Cfr10I 1693 GCCGGC [GA]^CCGG[CT] ClaI 1514 ATCGAT AT^CGAT DraIII 1288 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 858 GAAGAG CTCTTC(1/4) Eco47III 296 AGCGCT AGC^GCT Eco81I 381 CCTGAGG CC^T[ACGT]AGG FseI 1692 GGCCGGCC GGCCGG^CC HgaI 684 GCGTC GACGC(5/10) Ksp632I 858 GAAGAG CTCTTC(1/4) KspI 402 CCGCGG CCGC^GG MaeII 1475 ACGT A^CGT MamI 421 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 249 GGATCC [GA]^GATC[CT] MroI 878 TCCGGA T^CCGGA NaeI 1693 GCCGGC GCC^GGC NgoMIV 1693 GCCGGC G^CCGGC NspI 1377 GCATGT [GA]CATG^[CT] NspV 455 TTCGAA TT^CGAA PflMI 1402 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1657 GGGACCC [GA]G^G[AT]CC[CT] PshAI 1701 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 523 GAGCTC GAGCT^C SacII 402 CCGCGG CCGC^GG SapI 858 GAAGAGC GCTCTTC(1/4) SfuI 455 TTCGAA TT^CGAA SspI 913 AATATT AAT^ATT Van91I 1402 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 618 TCTAGA T^CTAGA XhoII 249 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 57 CGCG CG^CG 403 CGCG AcsI 1088 AAATTC [GA]^AATT[CT] 1302 GAATTT AlwNI 376 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 1570 CAGTACCTG ApaI 408 GGGCCC GGGCC^C 414 GGGCCC ApoI 1088 AAATTC [GA]^AATT[CT] 1302 GAATTT AspHI 195 GAGCAC G[AT]GC[AT]^C 523 GAGCTC BalI 1229 TGGCCA TGG^CCA 1629 TGGCCA BcgI 1182 CGATTCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1241 GCATTGATGTCG BsaI 132 GAGACC GGTCTC(1/5) 614 GGTCTC BsiEI 922 CGGCCG CG[GA][CT]^CG 1695 CGGCCG BsiHKAI 195 GAGCAC G[AT]GC[AT]^C 523 GAGCTC BspHI 264 TCATGA T^CATGA 959 TCATGA BspMI 1574 ACCTGC ACCTGC(4/8) 157 GCAGGT BstEII 303 GGTGACC G^GT[ACGT]ACC 1146 GGTGACC BstPI 303 GGTGACC G^GT[ACGT]ACC 1146 GGTGACC BstUI 57 CGCG CG^CG 403 CGCG EagI 922 CGGCCG C^GGCCG 1695 CGGCCG EclXI 922 CGGCCG C^GGCCG 1695 CGGCCG Eco52I 922 CGGCCG C^GGCCG 1695 CGGCCG EcoO65I 303 GGTGACC G^GT[ACGT]ACC 1146 GGTGACC EcoT14I 1275 CCATGG C^C[AT][AT]GG 1724 CCATGG EcoT22I 550 ATGCAT ATGCA^T 805 ATGCAT MluNI 1229 TGGCCA TGG^CCA 1629 TGGCCA MscI 1229 TGGCCA TGG^CCA 1629 TGGCCA MslI 505 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 960 CATGAAGGTG MvnI 57 CGCG CG^CG 403 CGCG NcoI 1275 CCATGG C^CATGG 1724 CCATGG NsiI 550 ATGCAT ATGCA^T 805 ATGCAT RcaI 264 TCATGA T^CATGA 959 TCATGA StyI 1275 CCATGG C^C[AT][AT]GG 1724 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI