(Circular) (Six-base) MAPSORT of: pFN21AE6774.seq Check: 1214 from: 1 to: 6481 pFN21AE6774 6481 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2151 3824 1937 Size: 214 1673 4594 Fragments arranged by size: 4594 1673 214 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2049 2882 4544 5928 6196 1632 Size: 417 833 1662 1384 268 1917 Fragments arranged by size: 1917 1662 1384 833 417 268 AfeI AGC'GCT Cuts at: 2303 2303 Size: 6481 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 5380 6357 1203 Size: 4177 977 1327 Fragments arranged by size: 4177 1327 977 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 5210 5210 Size: 6481 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6481 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6481 AlwNI CAG_nnn'CTG Cuts at: 2386 3580 4224 5796 2386 Size: 1194 644 1572 3071 Fragments arranged by size: 3071 1572 1194 644 ApaI G_GGCC'C Cuts at: 2417 2423 2417 Size: 6 6475 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 3093 3307 3790 4016 4168 6277 1493 Size: 1600 214 483 226 152 2109 1697 Fragments arranged by size: 2109 1697 1600 483 226 214 152 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 2074 2319 2416 3806 1870 Size: 73 131 245 97 1390 4545 Fragments arranged by size: 4545 1390 245 131 97 73 BamHI G'GATC_C Cuts at: 2254 3811 4178 2254 Size: 1557 367 4557 Fragments arranged by size: 4557 1557 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3802 5257 618 Size: 325 217 627 2015 1455 1842 Fragments arranged by size: 2015 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2083 2417 2423 2532 3396 3800 1391 Size: 692 334 6 109 864 404 4072 Fragments arranged by size: 4072 864 692 404 334 109 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5510 BbvCI CC'TCA_GC Cuts at: 3160 3160 Size: 6481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3176 3210 3235 3269 4672 4706 1903 Size: 34 1239 34 25 34 1403 34 3678 Fragments arranged by size: 3678 1403 1239 34 34 34 34 25 BciVI GTATCCnnnnn_n' Cuts at: 3278 4373 5583 3278 Size: 1095 1210 4176 Fragments arranged by size: 4176 1210 1095 BclI T'GATC_A Cuts at: 1548 1548 Size: 6481 BfrBI ATG'CAT Cuts at: 2557 2812 2557 Size: 255 6226 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 2412 3360 Size: 107 122 71 631 605 406 334 948 Cuts at: 3360 3595 5092 136 Size: 235 1497 1525 Fragments arranged by size: 1525 1497 948 631 605 406 334 235 122 107 71 BglII A'GATC_T Cuts at: 6476 6476 Size: 6481 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 2417 2423 2728 4542 5698 1790 Size: 627 6 305 1814 1156 2573 Fragments arranged by size: 2573 1814 1156 627 305 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2207 2789 3555 Size: 416 53 83 399 24 790 582 766 Cuts at: 3555 4212 5170 442 Size: 657 958 1753 Fragments arranged by size: 1753 958 790 766 657 582 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2599 2599 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2026 2148 2546 3105 3186 3380 3705 5141 Size: 787 122 398 559 81 194 325 1436 Cuts at: 5141 1239 Size: 2579 Fragments arranged by size: 2579 1436 787 559 398 325 194 122 81 Bpu10I CC'TnA_GC Cuts at: 2291 3160 2291 Size: 869 5612 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4606 5471 5769 4606 Size: 865 298 5318 Fragments arranged by size: 5318 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2131 2625 5144 882 Size: 617 632 494 2519 2219 Fragments arranged by size: 2519 2219 632 617 494 BsaAI yAC'GTr Cuts at: 493 1204 4272 493 Size: 711 3068 2702 Fragments arranged by size: 3068 2702 711 BsaBI GATnn'nnATC Cuts at: 2430 6475 2430 Size: 4045 2436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4288 4670 5305 275 Size: 53 83 186 936 2755 382 635 1451 Fragments arranged by size: 2755 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2883 4914 5312 5586 5733 6144 2883 Size: 2031 398 274 147 411 3220 Fragments arranged by size: 3220 2031 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3103 3133 1178 Size: 30 165 30 1700 30 4526 Fragments arranged by size: 4526 1700 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3332 1186 Size: 2146 4335 BseYI C'CCAG_C Cuts at: 2505 3389 5684 6180 2505 Size: 884 2295 496 2806 Fragments arranged by size: 2806 2295 884 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3439 1163 Size: 573 1703 4205 Fragments arranged by size: 4205 1703 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2930 3703 3931 4692 5720 1540 Size: 275 186 929 773 228 761 1028 2301 Fragments arranged by size: 2301 1028 929 773 761 275 228 186 BsiHKAI G_wGCw'C Cuts at: 2204 2532 4542 4627 5698 2204 Size: 328 2010 85 1071 2987 Fragments arranged by size: 2987 2010 1071 328 85 BsmI GAATG_Cn' Cuts at: 3234 3992 4085 3234 Size: 758 93 5630 Fragments arranged by size: 5630 758 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2204 2417 2423 2532 2728 3396 Size: 399 293 121 213 6 109 196 668 Cuts at: 3396 3800 4542 4627 5698 1391 Size: 404 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 668 404 399 293 213 196 121 109 85 6 BspEI T'CCGG_A Cuts at: 2883 6144 2883 Size: 3261 3220 BspHI T'CATG_A Cuts at: 2269 2964 4370 6100 2269 Size: 695 1406 1730 2650 Fragments arranged by size: 2650 1730 1406 695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2153 3588 3836 844 Size: 1309 1435 248 3489 Fragments arranged by size: 3489 1435 1309 248 BsrBI CCG'CTC Cuts at: 1394 1823 4368 1394 Size: 429 2545 3507 Fragments arranged by size: 3507 2545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3007 4976 5150 65 Size: 1183 1759 1969 174 1396 Fragments arranged by size: 1969 1759 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3698 5125 5312 1700 Size: 110 1888 1427 187 2869 Fragments arranged by size: 2869 1888 1427 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6481 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 3296 4541 5553 3296 Size: 1245 1012 4224 Fragments arranged by size: 4224 1245 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6481 BstBI TT'CG_AA Cuts at: 2461 5285 2461 Size: 2824 3657 BstEII G'GTnAC_C Cuts at: 1184 2308 3151 1184 Size: 1124 843 4514 Fragments arranged by size: 4514 1124 843 BstXI CCAn_nnnn'nTGG Cuts at: 2305 3330 3373 2305 Size: 1025 43 5413 Fragments arranged by size: 5413 1025 43 BstYI r'GATC_y Cuts at: 1972 2254 3811 4178 4563 4580 6032 6118 6130 Size: 282 1557 367 385 17 1452 86 12 Cuts at: 6130 6476 1972 Size: 346 1977 Fragments arranged by size: 1977 1557 1452 385 367 346 282 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2387 2387 Size: 6481 BtgI C'CryG_G Cuts at: 513 1065 2407 3280 3407 3729 513 Size: 552 1342 873 127 322 3265 Fragments arranged by size: 3265 1342 873 552 322 127 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2929 487 Size: 683 56 1703 4039 Fragments arranged by size: 4039 1703 683 56 BtsI GCAGTG_nn' Cuts at: 2684 3451 3997 4791 4811 2684 Size: 767 546 794 20 4354 Fragments arranged by size: 4354 794 767 546 20 ClaI AT'CG_AT Cuts at: 1554 3520 1554 Size: 1966 4515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 3785 3785 Size: 6481 DraIII CAC_nnn'GTG Cuts at: 3298 3298 Size: 6481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2815 2927 3022 Size: 54 1425 50 275 18 985 112 95 Cuts at: 3022 3234 3415 3634 3700 3928 4817 6446 8 Size: 212 181 219 66 228 889 1629 43 Fragments arranged by size: 1629 1425 985 889 275 228 219 212 181 112 95 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2927 3700 3928 1537 Size: 275 1115 773 228 4090 Fragments arranged by size: 4090 1115 773 275 228 EarI CTCTTCn'nnn_ Cuts at: 1353 2858 4411 1353 Size: 1505 1553 3423 Fragments arranged by size: 3423 1553 1505 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5068 5442 5588 387 Size: 1064 138 3479 374 146 1280 Fragments arranged by size: 3479 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2530 2530 Size: 6481 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2026 2049 2148 2546 2882 3105 3186 Size: 393 394 23 99 398 336 223 81 Cuts at: 3186 3380 3705 4544 5141 5928 6196 1239 Size: 194 325 839 597 787 268 1524 Fragments arranged by size: 1524 839 787 597 398 394 393 336 325 268 223 194 99 81 23 EcoO109I rG'GnC_Cy Cuts at: 2413 3604 3663 2413 Size: 1191 59 5231 Fragments arranged by size: 5231 1191 59 EcoRI G'AATT_C Cuts at: 3790 4168 3790 Size: 378 6103 FseI GG_CCGG'CC Cuts at: 3702 3702 Size: 6481 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 2305 2751 3355 5628 1678 Size: 627 446 604 2273 2531 Fragments arranged by size: 2531 2273 627 604 446 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2659 2691 3059 3091 3433 Size: 32 135 32 1087 32 368 32 342 Cuts at: 3433 3465 3535 3567 5122 5154 5196 5228 1373 Size: 32 70 32 1555 32 42 32 2626 Fragments arranged by size: 2626 1555 1087 368 342 135 70 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3825 4071 669 Size: 988 281 1887 246 3079 Fragments arranged by size: 3079 1887 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6481 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 4080 4080 Size: 6481 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5595 5779 1305 Size: 4290 184 2007 Fragments arranged by size: 4290 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3236 3636 10 Size: 54 1425 343 1404 400 2855 Fragments arranged by size: 2855 1425 1404 400 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2514 2969 4439 4798 4957 518 Size: 648 843 505 455 1470 359 159 2042 Fragments arranged by size: 2042 1470 843 648 505 455 359 159 MspA1I CmG'CkG Cuts at: 1606 2251 2409 2860 3164 3400 3897 4574 5722 Size: 645 158 451 304 236 497 677 1148 Cuts at: 5722 5967 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 645 497 451 304 245 236 158 NaeI GCC'GGC Cuts at: 3700 3700 Size: 6481 NcoI C'CATG_G Cuts at: 513 1065 3280 3729 513 Size: 552 2215 449 3265 Fragments arranged by size: 3265 2215 552 449 NdeI CA'TA_TG Cuts at: 387 387 Size: 6481 NgoMIV G'CCGG_C Cuts at: 3698 3698 Size: 6481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NsiI A_TGCA'T Cuts at: 2559 2814 2559 Size: 255 6226 NspI r_CATG'y Cuts at: 1132 1229 3386 3839 5384 1132 Size: 97 2157 453 1545 2229 Fragments arranged by size: 2229 2157 1545 453 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 5380 5380 Size: 6481 PflMI CCAn_nnn'nTGG Cuts at: 1108 3413 1108 Size: 2305 4176 PfoI T'CCnGG_A Cuts at: 2708 3121 3426 2708 Size: 413 305 5763 Fragments arranged by size: 5763 413 305 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2225 2257 4550 4582 6063 6095 2225 Size: 32 2293 32 1481 32 2611 Fragments arranged by size: 2611 2293 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3663 3663 Size: 6481 PshAI GACnn'nnGTC Cuts at: 1613 3710 1613 Size: 2097 4384 PsiI TTA'TAA Cuts at: 2637 4051 2637 Size: 1414 5067 PspOMI G'GGCC_C Cuts at: 2413 2419 2413 Size: 6 6475 PstI C_TGCA'G Cuts at: 830 2164 2250 2376 3202 3588 3833 830 Size: 1334 86 126 826 386 245 3478 Fragments arranged by size: 3478 1334 826 386 245 126 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 PvuII CAG'CTG Cuts at: 2251 2860 3164 2251 Size: 609 304 5568 Fragments arranged by size: 5568 609 304 SacI G_AGCT'C Cuts at: 2532 2532 Size: 6481 SacII CC_GC'GG Cuts at: 2410 2410 Size: 6481 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SanDI GG'GwC_CC Cuts at: 3663 3663 Size: 6481 SapI GCTCTTCn'nnn_ Cuts at: 2858 2858 Size: 6481 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 4729 6441 4729 Size: 1712 4769 SfcI C'TryA_G Cuts at: 826 1045 2148 2160 2246 2372 3198 3584 3829 Size: 219 1103 12 86 126 826 386 245 Cuts at: 3829 4964 5645 5836 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1103 826 681 386 245 219 191 126 86 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 2076 2321 2418 3808 1872 Size: 204 245 97 1390 4545 Fragments arranged by size: 4545 1390 245 204 97 SmlI C'TyrA_G Cuts at: 820 1017 1943 4585 5486 5748 820 Size: 197 926 2642 901 262 1553 Fragments arranged by size: 2642 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SrfI GCCC'GGGC Cuts at: 2418 2418 Size: 6481 SspI AAT'ATT Cuts at: 5 52 2920 4405 5 Size: 47 2868 1485 2081 Fragments arranged by size: 2868 2081 1485 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6481 StyI C'CwwG_G Cuts at: 513 1065 3280 3729 3920 513 Size: 552 2215 449 191 3074 Fragments arranged by size: 3074 2215 552 449 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2378 4508 4525 4678 4863 5308 325 Size: 1255 798 2130 17 153 185 445 1498 Fragments arranged by size: 2130 1498 1255 798 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 2760 2981 Size: 274 80 33 51 1443 765 18 221 Cuts at: 2981 4727 6439 96 Size: 1746 1712 138 Fragments arranged by size: 1746 1712 1443 765 274 221 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6441 6473 6441 Size: 32 6449 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6481 XbaI T'CTAG_A Cuts at: 2623 3817 2623 Size: 1194 5287 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6481 XmaI C'CCGG_G Cuts at: 1870 2074 2319 2416 3806 1870 Size: 204 245 97 1390 4545 Fragments arranged by size: 4545 1390 245 204 97 XmnI GAAnn'nnTTC Cuts at: 4610 4610 Size: 6481 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SexAI SfiI SfoI SwaI XcmI