Restriction map for
pFN21AE6774
(Linear) (Six-base) MAPSORT of: KIEE6774.seq Check: 9878 from: 1 to: 1776
KIEE6774.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 146 1776
Size: 146 1630
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 877 1776
Size: 44 833 899
Fragments arranged by size:
899 833 44
AfeI AGC'GCT
Cuts at: 0 298 1776
Size: 298 1478
AlwNI CAG_nnn'CTG
Cuts at: 0 381 1575 1776
Size: 381 1194 201
Fragments arranged by size:
1194 381 201
ApaI G_GGCC'C
Cuts at: 0 412 418 1776
Size: 412 6 1358
Fragments arranged by size:
1358 412 6
ApoI r'AATT_y
Cuts at: 0 1088 1302 1776
Size: 1088 214 474
Fragments arranged by size:
1088 474 214
AvaI C'yCGr_G
Cuts at: 0 69 314 411 1776
Size: 69 245 97 1365
Fragments arranged by size:
1365 245 97 69
BamHI G'GATC_C
Cuts at: 0 249 1776
Size: 249 1527
BanII G_rGCy'C
Cuts at: 0 78 412 418 527 1391 1776
Size: 78 334 6 109 864 385
Fragments arranged by size:
864 385 334 109 78 6
BbvCI CC'TCA_GC
Cuts at: 0 1155 1776
Size: 1155 621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1171 1205 1230 1264 1776
Size: 1171 34 25 34 512
Fragments arranged by size:
1171 512 34 34 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 1273 1776
Size: 1273 503
BfrBI ATG'CAT
Cuts at: 0 552 807 1776
Size: 552 255 969
Fragments arranged by size:
969 552 255
BglI GCCn_nnn'nGGC
Cuts at: 0 73 407 1355 1590 1776
Size: 73 334 948 235 186
Fragments arranged by size:
948 334 235 186 73
Bme1580I G_kGCm'C
Cuts at: 0 412 418 723 1776
Size: 412 6 305 1053
Fragments arranged by size:
1053 412 305 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 202 784 1550 1776
Size: 202 582 766 226
Fragments arranged by size:
766 582 226 202
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 594 1776
Size: 594 1182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 143 541 1100 1181 1375 1700 1776
Size: 21 122 398 559 81 194 325 76
Fragments arranged by size:
559 398 325 194 122 81 76 21
Bpu10I CC'TnA_GC
Cuts at: 0 286 1155 1776
Size: 286 869 621
Fragments arranged by size:
869 621 286
BsaI GGTCTCn'nnnn_
Cuts at: 0 126 620 1776
Size: 126 494 1156
Fragments arranged by size:
1156 494 126
BsaBI GATnn'nnATC
Cuts at: 0 425 1776
Size: 425 1351
BsaWI w'CCGG_w
Cuts at: 0 878 1776
Size: 878 898
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1098 1128 1776
Size: 1098 30 648
Fragments arranged by size:
1098 648 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1327 1776
Size: 1327 449
BseYI C'CCAG_C
Cuts at: 0 500 1384 1776
Size: 500 884 392
Fragments arranged by size:
884 500 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1434 1776
Size: 1434 342
BsiEI CG_ry'CG
Cuts at: 0 925 1698 1776
Size: 925 773 78
Fragments arranged by size:
925 773 78
BsiHKAI G_wGCw'C
Cuts at: 0 199 527 1776
Size: 199 328 1249
Fragments arranged by size:
1249 328 199
BsmI GAATG_Cn'
Cuts at: 0 1229 1776
Size: 1229 547
Bsp1286I G_dGCh'C
Cuts at: 0 78 199 412 418 527 723 1391 1776
Size: 78 121 213 6 109 196 668 385
Fragments arranged by size:
668 385 213 196 121 109 78 6
BspEI T'CCGG_A
Cuts at: 0 878 1776
Size: 878 898
BspHI T'CATG_A
Cuts at: 0 264 959 1776
Size: 264 695 817
Fragments arranged by size:
817 695 264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 148 1583 1776
Size: 148 1435 193
Fragments arranged by size:
1435 193 148
BsrDI GCAATG_nn'
Cuts at: 0 1002 1776
Size: 1002 774
BsrFI r'CCGG_y
Cuts at: 0 1693 1776
Size: 1693 83
BssSI C'ACGA_G
Cuts at: 0 1291 1776
Size: 1291 485
BstBI TT'CG_AA
Cuts at: 0 456 1776
Size: 456 1320
BstEII G'GTnAC_C
Cuts at: 0 303 1146 1776
Size: 303 843 630
Fragments arranged by size:
843 630 303
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 1325 1368 1776
Size: 300 1025 43 408
Fragments arranged by size:
1025 408 300 43
BstYI r'GATC_y
Cuts at: 0 249 1776
Size: 249 1527
Bsu36I CC'TnA_GG
Cuts at: 0 382 1776
Size: 382 1394
BtgI C'CryG_G
Cuts at: 0 402 1275 1402 1724 1776
Size: 402 873 127 322 52
Fragments arranged by size:
873 402 322 127 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 924 1776
Size: 924 852
BtsI GCAGTG_nn'
Cuts at: 0 679 1446 1776
Size: 679 767 330
Fragments arranged by size:
767 679 330
ClaI AT'CG_AT
Cuts at: 0 1515 1776
Size: 1515 261
DraIII CAC_nnn'GTG
Cuts at: 0 1293 1776
Size: 1293 483
EaeI y'GGCC_r
Cuts at: 0 810 922 1017 1229 1410 1629 1695 1776
Size: 810 112 95 212 181 219 66 81
Fragments arranged by size:
810 219 212 181 112 95 81 66
EagI C'GGCC_G
Cuts at: 0 922 1695 1776
Size: 922 773 81
Fragments arranged by size:
922 773 81
EarI CTCTTCn'nnn_
Cuts at: 0 853 1776
Size: 853 923
EcoICRI GAG'CTC
Cuts at: 0 525 1776
Size: 525 1251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 44 143 541 877 1100 1181 1375
Size: 21 23 99 398 336 223 81 194
Cuts at: 1375 1700 1776
Size: 325 76
Fragments arranged by size:
398 336 325 223 194 99 81 76
23 21
EcoO109I rG'GnC_Cy
Cuts at: 0 408 1599 1658 1776
Size: 408 1191 59 118
Fragments arranged by size:
1191 408 118 59
FseI GG_CCGG'CC
Cuts at: 0 1697 1776
Size: 1697 79
HaeII r_GCGC'y
Cuts at: 0 300 746 1350 1776
Size: 300 446 604 426
Fragments arranged by size:
604 446 426 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 654 686 1054 1086 1428 1460 1530 1562
Size: 654 32 368 32 342 32 70 32
Cuts at: 1562 1776
Size: 214
Fragments arranged by size:
654 368 342 214 70 32 32 32
32
MscI TGG'CCA
Cuts at: 0 1231 1631 1776
Size: 1231 400 145
Fragments arranged by size:
1231 400 145
MslI CAynn'nnrTG
Cuts at: 0 509 964 1776
Size: 509 455 812
Fragments arranged by size:
812 509 455
MspA1I CmG'CkG
Cuts at: 0 246 404 855 1159 1395 1776
Size: 246 158 451 304 236 381
Fragments arranged by size:
451 381 304 246 236 158
NaeI GCC'GGC
Cuts at: 0 1695 1776
Size: 1695 81
NcoI C'CATG_G
Cuts at: 0 1275 1724 1776
Size: 1275 449 52
Fragments arranged by size:
1275 449 52
NgoMIV G'CCGG_C
Cuts at: 0 1693 1776
Size: 1693 83
NsiI A_TGCA'T
Cuts at: 0 554 809 1776
Size: 554 255 967
Fragments arranged by size:
967 554 255
NspI r_CATG'y
Cuts at: 0 1381 1776
Size: 1381 395
PflMI CCAn_nnn'nTGG
Cuts at: 0 1408 1776
Size: 1408 368
PfoI T'CCnGG_A
Cuts at: 0 703 1116 1421 1776
Size: 703 413 305 355
Fragments arranged by size:
703 413 355 305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 1776
Size: 220 32 1524
Fragments arranged by size:
1524 220 32
PpuMI rG'GwC_Cy
Cuts at: 0 1658 1776
Size: 1658 118
PshAI GACnn'nnGTC
Cuts at: 0 1705 1776
Size: 1705 71
PsiI TTA'TAA
Cuts at: 0 632 1776
Size: 632 1144
PspOMI G'GGCC_C
Cuts at: 0 408 414 1776
Size: 408 6 1362
Fragments arranged by size:
1362 408 6
PstI C_TGCA'G
Cuts at: 0 159 245 371 1197 1583 1776
Size: 159 86 126 826 386 193
Fragments arranged by size:
826 386 193 159 126 86
PvuII CAG'CTG
Cuts at: 0 246 855 1159 1776
Size: 246 609 304 617
Fragments arranged by size:
617 609 304 246
SacI G_AGCT'C
Cuts at: 0 527 1776
Size: 527 1249
SacII CC_GC'GG
Cuts at: 0 405 1776
Size: 405 1371
SanDI GG'GwC_CC
Cuts at: 0 1658 1776
Size: 1658 118
SapI GCTCTTCn'nnn_
Cuts at: 0 853 1776
Size: 853 923
SfcI C'TryA_G
Cuts at: 0 143 155 241 367 1193 1579 1776
Size: 143 12 86 126 826 386 197
Fragments arranged by size:
826 386 197 143 126 86 12
SmaI CCC'GGG
Cuts at: 0 71 316 413 1776
Size: 71 245 97 1363
Fragments arranged by size:
1363 245 97 71
SrfI GCCC'GGGC
Cuts at: 0 413 1776
Size: 413 1363
SspI AAT'ATT
Cuts at: 0 915 1776
Size: 915 861
StyI C'CwwG_G
Cuts at: 0 1275 1724 1776
Size: 1275 449 52
Fragments arranged by size:
1275 449 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 373 1776
Size: 373 1403
TatI w'GTAC_w
Cuts at: 0 737 755 976 1776
Size: 737 18 221 800
Fragments arranged by size:
800 737 221 18
XbaI T'CTAG_A
Cuts at: 0 618 1776
Size: 618 1158
XmaI C'CCGG_G
Cuts at: 0 69 314 411 1776
Size: 69 245 97 1365
Fragments arranged by size:
1365 245 97 69
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApaI ApoI AvaI BamHI
BanII BbvCI BcgI BciVI BfrBI BglI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BssSI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoO109I FseI HaeII Hin4I MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI
PfoI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII
SacI SacII SanDI SapI SfcI SmaI SrfI SspI
StyI TaqII TatI XbaI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BclI BglII BlpI BmgBI
BmtI BpuEI BsaAI BsaHI BsiWI BsmBI BsrBI BsrGI
BssHII BstAPI BstZ17I CspCI DraI DrdI EciI EcoNI
EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NarI NdeI NheI
NotI NruI PacI PasI PciI PmeI PmlI PspXI
PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI StuI
SwaI TstI Tth111I XcmI XhoI XmnI ZraI