################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BbeI BbrPI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BspDI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MseI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 328 TCCGGA T^CCGGA AcyI 712 GGCGTC G[GA]^CG[CT]C AfaI 501 GTAC GT^AC AocI 77 CCTCAGG CC^T[ACGT]AGG AvaI 268 CTCGAG C^[CT]CG[GA]G AviII 950 TGCGCA TGC^GCA BamHI 851 GGATCC G^GATCC BanI 193 GGCACC G^G[CT][GA]CC BbsI 21 GTCTTC GAAGAC(2/6) BbvCI 934 GCTGAGG CCTCAGC(-5/-2) BcgI 763 CGACTGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 839 CCCGG CC^[GC]GG BfaI 319 CTAG C^TAG BpuAI 21 GTCTTC GAAGAC(2/6) BsaHI 712 GGCGTC G[GA]^CG[CT]C BsaWI 328 TCCGGA [AT]^CCGG[AT] BseAI 328 TCCGGA T^CCGGA BsoBI 268 CTCGAG C^[CT]CG[GA]G Bsp1407I 500 TGTACA T^GTACA BspEI 328 TCCGGA T^CCGGA BspMI 530 ACCTGC ACCTGC(4/8) BsrGI 500 TGTACA T^GTACA BstYI 851 GGATCC [GA]^GATC[CT] Bsu36I 77 CCTCAGG CC^T[ACGT]AGG DsaI 398 CCATGG C^C[GA][CT]GG Eco81I 77 CCTCAGG CC^T[ACGT]AGG FspI 950 TGCGCA TGC^GCA Hin1I 712 GGCGTC G[GA]^CG[CT]C MaeI 319 CTAG C^TAG MflI 851 GGATCC [GA]^GATC[CT] MroI 328 TCCGGA T^CCGGA MslI 399 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 839 CCCGG CC^[GC]GG NcoI 398 CCATGG C^CATGG NheI 318 GCTAGC G^CTAGC PaeR7I 268 CTCGAG C^TCGAG PleI 337 GAGTC GAGTC(4/5) PpuMI 6 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 754 CAGCTG CAG^CTG RsaI 501 GTAC GT^AC SspBI 500 TGTACA T^GTACA SspI 571 AATATT AAT^ATT TaqI 269 TCGA T^CGA TfiI 177 GAATC G^A[AT]TC TthHB8I 269 TCGA T^CGA XhoI 268 CTCGAG C^TCGAG XhoII 851 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 393 CGCG CG^CG 907 CGCG AlwNI 796 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG 919 CAGGAGCTG BalI 683 TGGCCA TGG^CCA 725 TGGCCA BlpI 39 GCTAAGC GC^T[ACGT]AGC 924 GCTGAGC BpmI 169 CTGGAG CTGGAG(16/14) 757 CTGGAG Bpu1102I 39 GCTAAGC GC^T[ACGT]AGC 924 GCTGAGC BsgI 181 CTGCAC GTGCAG(16/14) 771 CTGCAC BsmFI 8 GTCCC GGGAC(10/14) 508 GTCCC BsrDI 102 CATTGC GCAATG(2/0) 780 GCAATG BstEII 405 GGTGACC G^GT[ACGT]ACC 834 GGTGACC BstF5I 331 GGATG GGATG(2/0) 540 GGATG BstPI 405 GGTGACC G^GT[ACGT]ACC 834 GGTGACC BstUI 393 CGCG CG^CG 907 CGCG BstXI 56 CCAGGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 728 CCAATGTCCTGG CelII 39 GCTAAGC GC^T[ACGT]AGC 924 GCTGAGC DraII 6 GGGTCCC [GA]G^G[ACGT]CC[CT] 897 AGGCCCC EcoO109I 6 GGGTCCC [GA]G^G[ACGT]CC[CT] 897 AGGCCCC EcoO65I 405 GGTGACC G^GT[ACGT]ACC 834 GGTGACC FauI 931 CCCGC CCCGC(4/6) 106 GCGGG FokI 331 GGATG GGATG(9/13) 540 GGATG HaeII 263 AGCGCC [GA]GCGC^[CT] 842 GGCGCT HapII 329 CCGG C^CGG 840 CCGG HgaI 391 GACGC GACGC(5/10) 713 GCGTC HinfI 177 GAATC G^A[ACGT]TC 337 GAGTC HpaII 329 CCGG C^CGG 840 CCGG MluNI 683 TGGCCA TGG^CCA 725 TGGCCA MscI 683 TGGCCA TGG^CCA 725 TGGCCA MspI 329 CCGG C^CGG 840 CCGG MvnI 393 CGCG CG^CG 907 CGCG PstI 146 CTGCAG CTGCA^G 802 CTGCAG SfcI 146 CTGCAG C^T[GA][CT]AG 802 CTGCAG SmlI 268 CTCGAG C^T[CT][GA]AG 344 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I HphI ItaI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI XcmI