(Circular) (Six-base) MAPSORT of: pFN21AE6620.seq Check: 7104 from: 1 to: 5686 pFN21AE6620 5686 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3029 1937 Size: 1092 4594 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2888 3749 5133 5401 1632 Size: 1256 861 1384 268 1917 Fragments arranged by size: 1917 1384 1256 861 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5686 AlwNI CAG_nnn'CTG Cuts at: 2806 2929 3429 5001 2806 Size: 123 500 1572 3491 Fragments arranged by size: 3491 1572 500 123 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2995 3221 3373 5482 1493 Size: 1502 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 2273 3011 1870 Size: 73 330 738 4545 Fragments arranged by size: 4545 738 330 73 BamHI G'GATC_C Cuts at: 2856 3016 3383 2856 Size: 160 367 5159 Fragments arranged by size: 5159 367 160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2198 3007 4462 618 Size: 325 217 627 411 809 1455 1842 Fragments arranged by size: 1842 1455 809 627 411 325 217 BanII G_rGCy'C Cuts at: 1391 2234 2749 2936 3005 1391 Size: 843 515 187 69 4072 Fragments arranged by size: 4072 843 515 187 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 928 Size: 971 120 4595 Fragments arranged by size: 4595 971 120 BbvCI CC'TCA_GC Cuts at: 2940 2940 Size: 5686 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2757 2791 3877 3911 1903 Size: 34 50 34 736 34 1086 34 3678 Fragments arranged by size: 3678 1086 736 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 1548 Size: 5686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5681 5681 Size: 5686 BlpI GC'TnA_GC Cuts at: 2045 2930 3103 2045 Size: 885 173 4628 Fragments arranged by size: 4628 885 173 Bme1580I G_kGCm'C Cuts at: 1790 2201 3747 4903 1790 Size: 411 1546 1156 2573 Fragments arranged by size: 2573 1546 1156 411 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2204 3417 4375 Size: 416 53 83 399 24 787 1213 958 Cuts at: 4375 442 Size: 1753 Fragments arranged by size: 1753 1213 958 787 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2327 1056 Size: 1271 4415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2783 4346 1239 Size: 956 588 1563 2579 Fragments arranged by size: 2579 1563 956 588 Bpu10I CC'TnA_GC Cuts at: 2748 2940 2748 Size: 192 5494 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2334 3811 4676 4974 2334 Size: 1477 865 298 3046 Fragments arranged by size: 3046 1477 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4349 882 Size: 617 2850 2219 Fragments arranged by size: 2850 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2718 3493 3875 4510 Size: 53 83 186 936 1185 775 382 635 Cuts at: 4510 275 Size: 1451 Fragments arranged by size: 1451 1185 936 775 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2333 4119 4517 4791 4938 5349 2333 Size: 1786 398 274 147 411 2670 Fragments arranged by size: 2670 1786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2756 2786 1178 Size: 30 165 30 1353 30 4078 Fragments arranged by size: 4078 1353 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2957 2960 1186 Size: 1006 765 3 3912 Fragments arranged by size: 3912 1006 765 3 BseYI C'CCAG_C Cuts at: 2008 2515 2565 4889 5385 2008 Size: 507 50 2324 496 2309 Fragments arranged by size: 2324 2309 507 496 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2171 2761 1163 Size: 573 435 590 4088 Fragments arranged by size: 4088 590 573 435 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3747 3832 4903 3747 Size: 85 1071 4530 Fragments arranged by size: 4530 1071 85 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2234 2749 2936 3005 3747 3832 Size: 399 411 33 515 187 69 742 85 Cuts at: 3832 4903 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 515 411 399 187 85 69 33 BspEI T'CCGG_A Cuts at: 2333 5349 2333 Size: 3016 2670 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2544 3041 844 Size: 1700 497 3489 Fragments arranged by size: 3489 1700 497 BsrBI CCG'CTC Cuts at: 1394 1823 3573 1394 Size: 429 1750 3507 Fragments arranged by size: 3507 1750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2106 2792 4181 4355 65 Size: 1183 858 686 1389 174 1396 Fragments arranged by size: 1396 1389 1183 858 686 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4330 4517 1700 Size: 110 2520 187 2869 Fragments arranged by size: 2869 2520 187 110 BsrGI T'GTAC_A Cuts at: 96 2505 96 Size: 2409 3277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5686 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 2410 2839 1184 Size: 1226 429 4031 Fragments arranged by size: 4031 1226 429 BstXI CCAn_nnnn'nTGG Cuts at: 2068 2740 2068 Size: 672 5014 BstYI r'GATC_y Cuts at: 1972 2856 3016 3383 3768 3785 5237 5323 5335 Size: 884 160 367 385 17 1452 86 12 Cuts at: 5335 5681 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 884 385 367 346 160 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2083 2083 Size: 5686 BtgI C'CryG_G Cuts at: 513 1065 2403 513 Size: 552 1338 3796 Fragments arranged by size: 3796 1338 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 2142 2148 2269 2449 2556 2951 3202 3996 4016 Size: 6 121 180 107 395 251 794 20 Cuts at: 4016 2142 Size: 3812 Fragments arranged by size: 3812 794 395 251 180 121 107 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2478 2513 529 Size: 35 1914 35 3702 Fragments arranged by size: 3702 1914 35 35 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4693 809 Size: 507 3377 1802 Fragments arranged by size: 3377 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2400 2431 2688 Size: 54 1425 50 275 18 570 31 257 Cuts at: 2688 2730 3133 4022 5651 8 Size: 42 403 889 1629 43 Fragments arranged by size: 1629 1425 889 570 403 275 257 54 50 43 42 31 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3616 1353 Size: 2263 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4273 4647 4793 387 Size: 1064 138 2684 374 146 1280 Fragments arranged by size: 2684 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2783 2888 3749 4346 5133 5401 Size: 393 563 588 105 861 597 787 268 Cuts at: 5401 1239 Size: 1524 Fragments arranged by size: 1524 861 787 597 588 563 393 268 105 EcoO109I rG'GnC_Cy Cuts at: 2012 2903 2012 Size: 891 4795 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FspI TGC'GCA Cuts at: 2957 4192 2957 Size: 1235 4451 HaeII r_GCGC'y Cuts at: 1678 2272 2851 4833 1678 Size: 594 579 1982 2531 Fragments arranged by size: 2531 1982 594 579 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4327 4359 4401 4433 1373 Size: 32 135 32 2755 32 42 32 2626 Fragments arranged by size: 2755 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2690 2732 10 Size: 54 1425 343 858 42 2964 Fragments arranged by size: 2964 1425 858 343 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2408 3644 4003 4162 518 Size: 648 1242 1236 359 159 2042 Fragments arranged by size: 2042 1242 1236 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2598 2761 2939 3102 3779 4927 5172 1606 Size: 992 163 178 163 677 1148 245 2120 Fragments arranged by size: 2120 1148 992 677 245 178 163 163 NcoI C'CATG_G Cuts at: 513 1065 2403 513 Size: 552 1338 3796 Fragments arranged by size: 3796 1338 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NheI G'CTAG_C Cuts at: 1052 2323 1052 Size: 1271 4415 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 3044 4589 1132 Size: 97 1815 1545 2229 Fragments arranged by size: 2229 1815 1545 97 PasI CC'CwG_GG Cuts at: 1348 2861 1348 Size: 1513 4173 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5686 PfoI T'CCnGG_A Cuts at: 2060 2060 Size: 5686 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2012 2012 Size: 5686 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PspXI vC'TCGA_Gb Cuts at: 2273 2273 Size: 5686 PstI C_TGCA'G Cuts at: 830 2155 2811 3038 830 Size: 1325 656 227 3478 Fragments arranged by size: 3478 1325 656 227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 PvuII CAG'CTG Cuts at: 2761 2761 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SanDI GG'GwC_CC Cuts at: 2012 2012 Size: 5686 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SfcI C'TryA_G Cuts at: 826 1045 2151 2807 3034 4169 4850 5041 826 Size: 219 1106 656 227 1135 681 191 1471 Fragments arranged by size: 1471 1135 1106 681 656 227 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2273 2349 3790 4691 4953 820 Size: 197 926 330 76 1441 901 262 1553 Fragments arranged by size: 1553 1441 926 901 330 262 197 76 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 3044 1132 Size: 1912 3774 SspI AAT'ATT Cuts at: 5 52 2578 3610 5 Size: 47 2526 1032 2081 Fragments arranged by size: 2526 2081 1032 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5686 StyI C'CwwG_G Cuts at: 513 1065 2403 2469 2700 3125 513 Size: 552 1338 66 231 425 3074 Fragments arranged by size: 3074 1338 552 425 231 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 3730 3883 4068 4513 325 Size: 1255 2133 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2505 3932 5644 Size: 274 80 33 51 1443 528 1427 1712 Cuts at: 5644 96 Size: 138 Fragments arranged by size: 1712 1443 1427 528 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2756 2788 5646 5678 2756 Size: 32 2858 32 2764 Fragments arranged by size: 2858 2764 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2155 2234 2527 2698 2155 Size: 79 293 171 5143 Fragments arranged by size: 5143 293 171 79 XhoI C'TCGA_G Cuts at: 1943 2273 1943 Size: 330 5356 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 3815 3815 Size: 5686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI