(Linear) (Six-base) MAPSORT of: KIEE6620.seq Check: 8827 from: 1 to: 981 KIEE6620.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 883 981 Size: 883 98 AlwNI CAG_nnn'CTG Cuts at: 0 801 924 981 Size: 801 123 57 Fragments arranged by size: 801 123 57 AvaI C'yCGr_G Cuts at: 0 268 981 Size: 268 713 BamHI G'GATC_C Cuts at: 0 851 981 Size: 851 130 BanI G'GyrC_C Cuts at: 0 193 981 Size: 193 788 BanII G_rGCy'C Cuts at: 0 229 744 931 981 Size: 229 515 187 50 Fragments arranged by size: 515 229 187 50 BbsI GAAGACnn'nnnn_ Cuts at: 0 14 981 Size: 14 967 BbvCI CC'TCA_GC Cuts at: 0 935 981 Size: 935 46 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 752 786 981 Size: 752 34 195 Fragments arranged by size: 752 195 34 BlpI GC'TnA_GC Cuts at: 0 40 925 981 Size: 40 885 56 Fragments arranged by size: 885 56 40 Bme1580I G_kGCm'C Cuts at: 0 196 981 Size: 196 785 BmrI ACTGGGnnnn_n' Cuts at: 0 199 981 Size: 199 782 BmtI G_CTAG'C Cuts at: 0 322 981 Size: 322 659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 778 981 Size: 190 588 203 Fragments arranged by size: 588 203 190 Bpu10I CC'TnA_GC Cuts at: 0 743 935 981 Size: 743 192 46 Fragments arranged by size: 743 192 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 329 981 Size: 329 652 BsaHI Gr'CG_yC Cuts at: 0 713 981 Size: 713 268 BsaWI w'CCGG_w Cuts at: 0 328 981 Size: 328 653 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 751 781 981 Size: 751 30 200 Fragments arranged by size: 751 200 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 187 952 955 981 Size: 187 765 3 26 Fragments arranged by size: 765 187 26 3 BseYI C'CCAG_C Cuts at: 0 3 510 560 981 Size: 3 507 50 421 Fragments arranged by size: 507 421 50 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 756 981 Size: 166 590 225 Fragments arranged by size: 590 225 166 Bsp1286I G_dGCh'C Cuts at: 0 196 229 744 931 981 Size: 196 33 515 187 50 Fragments arranged by size: 515 196 187 50 33 BspEI T'CCGG_A Cuts at: 0 328 981 Size: 328 653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 539 981 Size: 539 442 BsrDI GCAATG_nn' Cuts at: 0 101 787 981 Size: 101 686 194 Fragments arranged by size: 686 194 101 BsrGI T'GTAC_A Cuts at: 0 500 981 Size: 500 481 BstEII G'GTnAC_C Cuts at: 0 405 834 981 Size: 405 429 147 Fragments arranged by size: 429 405 147 BstXI CCAn_nnnn'nTGG Cuts at: 0 63 735 981 Size: 63 672 246 Fragments arranged by size: 672 246 63 BstYI r'GATC_y Cuts at: 0 851 981 Size: 851 130 Bsu36I CC'TnA_GG Cuts at: 0 78 981 Size: 78 903 BtgI C'CryG_G Cuts at: 0 398 981 Size: 398 583 BtsI GCAGTG_nn' Cuts at: 0 137 143 264 444 551 946 981 Size: 137 6 121 180 107 395 35 Fragments arranged by size: 395 180 137 121 107 35 6 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 473 508 981 Size: 473 35 473 Fragments arranged by size: 473 473 35 EaeI y'GGCC_r Cuts at: 0 395 426 683 725 981 Size: 395 31 257 42 256 Fragments arranged by size: 395 257 256 42 31 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 778 883 981 Size: 190 588 105 98 Fragments arranged by size: 588 190 105 98 EcoO109I rG'GnC_Cy Cuts at: 0 7 898 981 Size: 7 891 83 Fragments arranged by size: 891 83 7 FspI TGC'GCA Cuts at: 0 952 981 Size: 952 29 HaeII r_GCGC'y Cuts at: 0 267 846 981 Size: 267 579 135 Fragments arranged by size: 579 267 135 MscI TGG'CCA Cuts at: 0 685 727 981 Size: 685 42 254 Fragments arranged by size: 685 254 42 MslI CAynn'nnrTG Cuts at: 0 403 981 Size: 403 578 MspA1I CmG'CkG Cuts at: 0 593 756 934 981 Size: 593 163 178 47 Fragments arranged by size: 593 178 163 47 NcoI C'CATG_G Cuts at: 0 398 981 Size: 398 583 NheI G'CTAG_C Cuts at: 0 318 981 Size: 318 663 PasI CC'CwG_GG Cuts at: 0 856 981 Size: 856 125 PfoI T'CCnGG_A Cuts at: 0 55 981 Size: 55 926 PpuMI rG'GwC_Cy Cuts at: 0 7 981 Size: 7 974 PspXI vC'TCGA_Gb Cuts at: 0 268 981 Size: 268 713 PstI C_TGCA'G Cuts at: 0 150 806 981 Size: 150 656 175 Fragments arranged by size: 656 175 150 PvuII CAG'CTG Cuts at: 0 756 981 Size: 756 225 SanDI GG'GwC_CC Cuts at: 0 7 981 Size: 7 974 SfcI C'TryA_G Cuts at: 0 146 802 981 Size: 146 656 179 Fragments arranged by size: 656 179 146 SmlI C'TyrA_G Cuts at: 0 268 344 981 Size: 268 76 637 Fragments arranged by size: 637 268 76 SspI AAT'ATT Cuts at: 0 573 981 Size: 573 408 StyI C'CwwG_G Cuts at: 0 398 464 695 981 Size: 398 66 231 286 Fragments arranged by size: 398 286 231 66 TatI w'GTAC_w Cuts at: 0 500 981 Size: 500 481 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 751 783 981 Size: 751 32 198 Fragments arranged by size: 751 198 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 150 229 522 693 981 Size: 150 79 293 171 288 Fragments arranged by size: 293 288 171 150 79 XhoI C'TCGA_G Cuts at: 0 268 981 Size: 268 713 Enzymes that do cut: AcuI AlwNI AvaI BamHI BanI BanII BbsI BbvCI BcgI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspEI BspMI BsrDI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI Eco57MI EcoO109I FspI HaeII MscI MslI MspA1I NcoI NheI PasI PfoI PpuMI PspXI PstI PvuII SanDI SfcI SmlI SspI StyI TatI TstI XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglI BglII BmgBI BplI BsaI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I XbaI XmaI XmnI ZraI