Restriction map for
pFN21AE1772
(Circular) (Six-base) MAPSORT of: pFN21AE1772.seq Check: 3561 from: 1 to: 5542
pFN21AE1772 5542 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3352 278
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 1937
Size: 948 4594
Acc65I G'GTAC_C
Cuts at: 2863 2863
Size: 5542
AclI AA'CG_TT
Cuts at: 1563 3669 4042 1563
Size: 2106 373 3063
Fragments arranged by size:
3063 2106 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3605 4989 5257 1632
Size: 1973 1384 268 1917
Fragments arranged by size:
1973 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5345
AflIII A'CryG_T
Cuts at: 1203 4441 5418 1203
Size: 3238 977 1327
Fragments arranged by size:
3238 1327 977
AgeI A'CCGG_T
Cuts at: 4373 4373
Size: 5542
AhdI GACnn_n'nnGTC
Cuts at: 4271 4271
Size: 5542
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2703 1184
Size: 1519 4023
AlwNI CAG_nnn'CTG
Cuts at: 2494 3285 4857 2494
Size: 791 1572 3179
Fragments arranged by size:
3179 1572 791
ApaI G_GGCC'C
Cuts at: 2202 2224 2232 2318 2202
Size: 22 8 86 5426
Fragments arranged by size:
5426 86 22 8
ApaLI G'TGCA_C
Cuts at: 3599 4755 3599
Size: 1156 4386
ApoI r'AATT_y
Cuts at: 1493 2851 3077 3229 5338 1493
Size: 1358 226 152 2109 1697
Fragments arranged by size:
2109 1697 1358 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4096 160
Size: 872 3064 1606
Fragments arranged by size:
3064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 2203 2224 2867 1870
Size: 73 170 90 21 643 4545
Fragments arranged by size:
4545 643 170 90 73 21
BamHI G'GATC_C
Cuts at: 2872 3239 2872
Size: 367 5175
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2209 2245 2275 2320 2528
Size: 325 217 627 422 36 30 45 208
Cuts at: 2528 2786 2863 4318 618
Size: 258 77 1455 1842
Fragments arranged by size:
1842 1455 627 422 325 258 217 208
77 45 36 30
BanII G_rGCy'C
Cuts at: 1391 2202 2224 2232 2318 2403 2861 1391
Size: 811 22 8 86 85 458 4072
Fragments arranged by size:
4072 811 458 86 85 22 8
BbeI G_GCGC'C
Cuts at: 2213 2324 2532 2790 2213
Size: 111 208 258 4965
Fragments arranged by size:
4965 258 208 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3733 3767 1903
Size: 34 50 34 1712 34 3678
Fragments arranged by size:
3678 1712 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3434 4644 3434
Size: 1210 4332
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2197 4153 136
Size: 107 122 71 631 605 525 1956 1525
Fragments arranged by size:
1956 1525 631 605 525 122 107 71
BglII A'GATC_T
Cuts at: 5537 5537
Size: 5542
BlpI GC'TnA_GC
Cuts at: 2959 2959
Size: 5542
Bme1580I G_kGCm'C
Cuts at: 1790 2202 2224 2232 2318 3603 4759 1790
Size: 412 22 8 86 1285 1156 2573
Fragments arranged by size:
2573 1285 1156 412 86 22 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5542
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3273 4231 442
Size: 416 53 83 399 24 1856 958 1753
Fragments arranged by size:
1856 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5542
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2537
Size: 5542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4202 1239
Size: 2963 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3667 4532 4830 3667
Size: 865 298 4379
Fragments arranged by size:
4379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4205 882
Size: 617 2706 2219
Fragments arranged by size:
2706 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2687 3333 493
Size: 711 1483 646 2702
Fragments arranged by size:
2702 1483 711 646
BsaBI GATnn'nnATC
Cuts at: 5536 5536
Size: 5542
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 2321 2529 2787
Size: 53 83 186 936 677 111 208 258
Cuts at: 2787 3349 3731 4366 275
Size: 562 382 635 1451
Fragments arranged by size:
1451 936 677 635 562 382 258 208
186 111 83 53
BsaWI w'CCGG_w
Cuts at: 3975 4373 4647 4794 5205 3975
Size: 398 274 147 411 4312
Fragments arranged by size:
4312 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2694 2724 1178
Size: 30 165 30 1291 30 3996
Fragments arranged by size:
3996 1291 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2057 2078 2081 1186
Size: 850 21 21 3 4647
Fragments arranged by size:
4647 850 21 21 3
BseYI C'CCAG_C
Cuts at: 2104 2414 4745 5241 2104
Size: 310 2331 496 2405
Fragments arranged by size:
2405 2331 496 310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4969
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2362 2992 3753 4781 1540
Size: 275 186 361 630 761 1028 2301
Fragments arranged by size:
2301 1028 761 630 361 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 2628 3603 3688 4759 2403
Size: 225 975 85 1071 3186
Fragments arranged by size:
3186 1071 975 225 85
BsmI GAATG_Cn'
Cuts at: 3053 3146 3053
Size: 93 5449
BsmBI CGTCTCn'nnnn_
Cuts at: 2144 2144
Size: 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2224 2232 2318 2403 2628 2861
Size: 399 412 22 8 86 85 225 233
Cuts at: 2861 3603 3688 4759 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 233 225 86
85 85 22 8
BspEI T'CCGG_A
Cuts at: 5205 5205
Size: 5542
BspHI T'CATG_A
Cuts at: 3431 5161 3431
Size: 1730 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2897 844
Size: 2053 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2710 3429 1394
Size: 429 887 719 3507
Fragments arranged by size:
3507 887 719 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4037 4211 65
Size: 1183 2789 174 1396
Fragments arranged by size:
2789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 2127 2248 2508 2604 4186 4373
Size: 110 226 91 121 260 96 1582 187
Cuts at: 4373 1700
Size: 2869
Fragments arranged by size:
2869 1582 260 226 187 121 110 96
91
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5542
BssHII G'CGCG_C
Cuts at: 1925 2418 1925
Size: 493 5049
BssSI C'ACGA_G
Cuts at: 3602 4614 3602
Size: 1012 4530
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2623 794
Size: 1829 3713
BstBI TT'CG_AA
Cuts at: 4346 4346
Size: 5542
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5542
BstYI r'GATC_y
Cuts at: 1972 2872 3239 3624 3641 5093 5179 5191 5537
Size: 900 367 385 17 1452 86 12 346
Cuts at: 5537 1972
Size: 1977
Fragments arranged by size:
1977 1452 900 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2439 2439
Size: 5542
BtgI C'CryG_G
Cuts at: 513 1065 2356 513
Size: 552 1291 3699
Fragments arranged by size:
3699 1291 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4803
Fragments arranged by size:
4803 683 56
BtsI GCAGTG_nn'
Cuts at: 2429 2782 3058 3852 3872 2429
Size: 353 276 794 20 4099
Fragments arranged by size:
4099 794 353 276 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5507
DraI TTT'AAA
Cuts at: 2846 2846
Size: 5542
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4549 809
Size: 507 3233 1802
Fragments arranged by size:
3233 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2359 2394 2989
Size: 54 1425 50 275 18 529 35 595
Cuts at: 2989 3878 5507 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 595 529 275 54 50
43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2359 2989 1537
Size: 275 547 630 4090
Fragments arranged by size:
4090 630 547 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2055 3472 1353
Size: 669 33 1417 3423
Fragments arranged by size:
3423 1417 669 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 2267 2318 4129 4503 4649
Size: 1064 138 474 204 51 1811 374 146
Cuts at: 4649 387
Size: 1280
Fragments arranged by size:
1811 1280 1064 474 374 204 146 138
51
EcoICRI GAG'CTC
Cuts at: 2401 2401
Size: 5542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3605 4202 4989 5257 1239
Size: 393 1973 597 787 268 1524
Fragments arranged by size:
1973 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 2794 2642
Size: 152 5390
EcoO109I rG'GnC_Cy
Cuts at: 2199 2221 2228 2229 2265 2332 2411 2565 2717
Size: 22 7 1 36 67 79 154 152
Cuts at: 2717 2199
Size: 5024
Fragments arranged by size:
5024 154 152 79 67 36 22 7
1
EcoRI G'AATT_C
Cuts at: 2851 3229 2851
Size: 378 5164
FspI TGC'GCA
Cuts at: 4048 4048
Size: 5542
HaeII r_GCGC'y
Cuts at: 1678 2105 2213 2324 2532 2790 4689 1678
Size: 427 108 111 208 258 1899 2531
Fragments arranged by size:
2531 1899 427 258 208 111 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4183 4215 4257 4289 1373
Size: 32 135 32 2611 32 42 32 2626
Fragments arranged by size:
2626 2611 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2886 3132 669
Size: 988 281 948 246 3079
Fragments arranged by size:
3079 988 948 281 246
HindIII A'AGCT_T
Cuts at: 748 2693 748
Size: 1945 3597
HpaI GTT'AAC
Cuts at: 3132 3132
Size: 5542
KasI G'GCGC_C
Cuts at: 2209 2320 2528 2786 2209
Size: 111 208 258 4965
Fragments arranged by size:
4965 258 208 111
KpnI G_GTAC'C
Cuts at: 2867 2867
Size: 5542
MfeI C'AATT_G
Cuts at: 3141 3141
Size: 5542
MluI A'CGCG_T
Cuts at: 5418 5418
Size: 5542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4656 4840 1305
Size: 3351 184 2007
Fragments arranged by size:
3351 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3720
Fragments arranged by size:
3720 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3500 3859 4018 518
Size: 648 2334 359 159 2042
Fragments arranged by size:
2334 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2215 2281 2358 2494 2958 3635 4783
Size: 555 54 66 77 136 464 677 1148
Cuts at: 4783 5028 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 555 464 245 136 77
66 54
NaeI GCC'GGC
Cuts at: 2038 2250 2510 2038
Size: 212 260 5070
Fragments arranged by size:
5070 260 212
NarI GG'CG_CC
Cuts at: 2210 2321 2529 2787 2210
Size: 111 208 258 4965
Fragments arranged by size:
4965 258 208 111
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4990
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5542
NgoMIV G'CCGG_C
Cuts at: 2036 2248 2508 2036
Size: 212 260 5070
Fragments arranged by size:
5070 260 212
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5542
NotI GC'GGCC_GC
Cuts at: 2359 2989 2359
Size: 630 4912
NruI TCG'CGA
Cuts at: 2671 2671
Size: 5542
NspI r_CATG'y
Cuts at: 1132 1229 2900 4445 1132
Size: 97 1671 1545 2229
Fragments arranged by size:
2229 1671 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2327 2347 1348
Size: 979 20 4543
Fragments arranged by size:
4543 979 20
PciI A'CATG_T
Cuts at: 4441 4441
Size: 5542
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5542
PfoI T'CCnGG_A
Cuts at: 2461 2792 2461
Size: 331 5211
PmeI GTTT'AAAC
Cuts at: 2846 2846
Size: 5542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 5124 5156 3611
Size: 32 1481 32 3997
Fragments arranged by size:
3997 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2265 2332 2565 2717 2265
Size: 67 233 152 5090
Fragments arranged by size:
5090 233 152 67
PshAI GACnn'nnGTC
Cuts at: 1613 2563 2722 1613
Size: 950 159 4433
Fragments arranged by size:
4433 950 159
PsiI TTA'TAA
Cuts at: 3112 3112
Size: 5542
PspOMI G'GGCC_C
Cuts at: 2198 2220 2228 2314 2198
Size: 22 8 86 5426
Fragments arranged by size:
5426 86 22 8
PstI C_TGCA'G
Cuts at: 830 2091 2160 2553 2616 2682 2777 2894 830
Size: 1261 69 393 63 66 95 117 3478
Fragments arranged by size:
3478 1261 393 117 95 69 66 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5542
PvuII CAG'CTG
Cuts at: 2161 2161
Size: 5542
SacI G_AGCT'C
Cuts at: 2403 2403
Size: 5542
SacII CC_GC'GG
Cuts at: 2359 2359
Size: 5542
SalI G'TCGA_C
Cuts at: 1936 2884 1936
Size: 948 4594
SanDI GG'GwC_CC
Cuts at: 2565 2565
Size: 5542
SbfI CC_TGCA'GG
Cuts at: 2894 2894
Size: 5542
ScaI AGT'ACT
Cuts at: 3790 5502 3790
Size: 1712 3830
SfcI C'TryA_G
Cuts at: 826 1045 2087 2156 2549 2612 2678 2773 2890
Size: 219 1042 69 393 63 66 95 117
Cuts at: 2890 4025 4706 4897 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1042 681 393 219 191 117
95 69 66 63
SfoI GGC'GCC
Cuts at: 2211 2322 2530 2788 2211
Size: 111 208 258 4965
Fragments arranged by size:
4965 258 208 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5542
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5542
SmaI CCC'GGG
Cuts at: 1872 2205 2226 2869 1872
Size: 333 21 643 4545
Fragments arranged by size:
4545 643 333 21
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3646 4547 4809 820
Size: 197 926 1703 901 262 1553
Fragments arranged by size:
1703 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3333 493
Size: 2840 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5542
SphI G_CATG'C
Cuts at: 1132 2900 1132
Size: 1768 3774
SspI AAT'ATT
Cuts at: 5 52 3466 5
Size: 47 3414 2081
Fragments arranged by size:
3414 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5542
StyI C'CwwG_G
Cuts at: 513 1065 2981 513
Size: 552 1916 3074
Fragments arranged by size:
3074 1916 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3569 3586 3739 3924 4369 325
Size: 1255 1989 17 153 185 445 1498
Fragments arranged by size:
1989 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3788 5500 96
Size: 274 80 33 51 1443 1811 1712 138
Fragments arranged by size:
1811 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5502 5534 5502
Size: 32 5510
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5542
XbaI T'CTAG_A
Cuts at: 2878 2878
Size: 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5542
XmaI C'CCGG_G
Cuts at: 1870 2203 2224 2867 1870
Size: 333 21 643 4545
Fragments arranged by size:
4545 643 333 21
XmnI GAAnn'nnTTC
Cuts at: 3671 3671
Size: 5542
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3350 276
Size: 53 83 186 936 1816 2468
Fragments arranged by size:
2468 1816 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI
FspAI NsiI PacI PmlI PspXI PsrI RsrII SapI
SexAI SfiI SrfI SwaI XcmI