Restriction map for
pFN21AE1772
(Linear) (Six-base) MAPSORT of: KIEE1772.seq Check: 8700 from: 1 to: 837
KIEE1772.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 698 837
Size: 698 139
AlwNI CAG_nnn'CTG
Cuts at: 0 489 837
Size: 489 348
ApaI G_GGCC'C
Cuts at: 0 197 219 227 313 837
Size: 197 22 8 86 524
Fragments arranged by size:
524 197 86 22 8
AvaI C'yCGr_G
Cuts at: 0 108 198 219 837
Size: 108 90 21 618
Fragments arranged by size:
618 108 90 21
BanI G'GyrC_C
Cuts at: 0 204 240 270 315 523 781 837
Size: 204 36 30 45 208 258 56
Fragments arranged by size:
258 208 204 56 45 36 30
BanII G_rGCy'C
Cuts at: 0 197 219 227 313 398 837
Size: 197 22 8 86 85 439
Fragments arranged by size:
439 197 86 85 22 8
BbeI G_GCGC'C
Cuts at: 0 208 319 527 785 837
Size: 208 111 208 258 52
Fragments arranged by size:
258 208 208 111 52
BglI GCCn_nnn'nGGC
Cuts at: 0 192 837
Size: 192 645
Bme1580I G_kGCm'C
Cuts at: 0 197 219 227 313 837
Size: 197 22 8 86 524
Fragments arranged by size:
524 197 86 22 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 837
Size: 532 305
BsaAI yAC'GTr
Cuts at: 0 682 837
Size: 682 155
BsaHI Gr'CG_yC
Cuts at: 0 205 316 524 782 837
Size: 205 111 208 258 55
Fragments arranged by size:
258 208 205 111 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 689 719 837
Size: 689 30 118
Fragments arranged by size:
689 118 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 52 73 76 837
Size: 31 21 21 3 761
Fragments arranged by size:
761 31 21 21 3
BseYI C'CCAG_C
Cuts at: 0 99 409 837
Size: 99 310 428
Fragments arranged by size:
428 310 99
BsiEI CG_ry'CG
Cuts at: 0 357 837
Size: 357 480
BsiHKAI G_wGCw'C
Cuts at: 0 398 623 837
Size: 398 225 214
Fragments arranged by size:
398 225 214
BsmBI CGTCTCn'nnnn_
Cuts at: 0 139 837
Size: 139 698
Bsp1286I G_dGCh'C
Cuts at: 0 197 219 227 313 398 623 837
Size: 197 22 8 86 85 225 214
Fragments arranged by size:
225 214 197 86 85 22 8
BsrBI CCG'CTC
Cuts at: 0 705 837
Size: 705 132
BsrFI r'CCGG_y
Cuts at: 0 31 122 243 503 599 837
Size: 31 91 121 260 96 238
Fragments arranged by size:
260 238 121 96 91 31
BssHII G'CGCG_C
Cuts at: 0 413 837
Size: 413 424
BstAPI GCAn_nnn'nTGC
Cuts at: 0 618 837
Size: 618 219
Bsu36I CC'TnA_GG
Cuts at: 0 434 837
Size: 434 403
BtgI C'CryG_G
Cuts at: 0 351 837
Size: 351 486
BtsI GCAGTG_nn'
Cuts at: 0 424 777 837
Size: 424 353 60
Fragments arranged by size:
424 353 60
EaeI y'GGCC_r
Cuts at: 0 354 389 837
Size: 354 35 448
Fragments arranged by size:
448 354 35
EagI C'GGCC_G
Cuts at: 0 354 837
Size: 354 483
EarI CTCTTCn'nnn_
Cuts at: 0 17 50 837
Size: 17 33 787
Fragments arranged by size:
787 33 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 262 313 837
Size: 58 204 51 524
Fragments arranged by size:
524 204 58 51
EcoICRI GAG'CTC
Cuts at: 0 396 837
Size: 396 441
EcoNI CCTnn'n_nnAGG
Cuts at: 0 637 789 837
Size: 637 152 48
Fragments arranged by size:
637 152 48
EcoO109I rG'GnC_Cy
Cuts at: 0 194 216 223 224 260 327 406 560
Size: 194 22 7 1 36 67 79 154
Cuts at: 560 712 837
Size: 152 125
Fragments arranged by size:
194 154 152 125 79 67 36 22
7 1
HaeII r_GCGC'y
Cuts at: 0 100 208 319 527 785 837
Size: 100 108 111 208 258 52
Fragments arranged by size:
258 208 111 108 100 52
HindIII A'AGCT_T
Cuts at: 0 688 837
Size: 688 149
KasI G'GCGC_C
Cuts at: 0 204 315 523 781 837
Size: 204 111 208 258 56
Fragments arranged by size:
258 208 204 111 56
MspA1I CmG'CkG
Cuts at: 0 156 210 276 353 489 837
Size: 156 54 66 77 136 348
Fragments arranged by size:
348 156 136 77 66 54
NaeI GCC'GGC
Cuts at: 0 33 245 505 837
Size: 33 212 260 332
Fragments arranged by size:
332 260 212 33
NarI GG'CG_CC
Cuts at: 0 205 316 524 782 837
Size: 205 111 208 258 55
Fragments arranged by size:
258 208 205 111 55
NgoMIV G'CCGG_C
Cuts at: 0 31 243 503 837
Size: 31 212 260 334
Fragments arranged by size:
334 260 212 31
NotI GC'GGCC_GC
Cuts at: 0 354 837
Size: 354 483
NruI TCG'CGA
Cuts at: 0 666 837
Size: 666 171
PasI CC'CwG_GG
Cuts at: 0 322 342 837
Size: 322 20 495
Fragments arranged by size:
495 322 20
PfoI T'CCnGG_A
Cuts at: 0 456 787 837
Size: 456 331 50
Fragments arranged by size:
456 331 50
PpuMI rG'GwC_Cy
Cuts at: 0 260 327 560 712 837
Size: 260 67 233 152 125
Fragments arranged by size:
260 233 152 125 67
PshAI GACnn'nnGTC
Cuts at: 0 558 717 837
Size: 558 159 120
Fragments arranged by size:
558 159 120
PspOMI G'GGCC_C
Cuts at: 0 193 215 223 309 837
Size: 193 22 8 86 528
Fragments arranged by size:
528 193 86 22 8
PstI C_TGCA'G
Cuts at: 0 86 155 548 611 677 772 837
Size: 86 69 393 63 66 95 65
Fragments arranged by size:
393 95 86 69 66 65 63
PvuII CAG'CTG
Cuts at: 0 156 837
Size: 156 681
SacI G_AGCT'C
Cuts at: 0 398 837
Size: 398 439
SacII CC_GC'GG
Cuts at: 0 354 837
Size: 354 483
SanDI GG'GwC_CC
Cuts at: 0 560 837
Size: 560 277
SfcI C'TryA_G
Cuts at: 0 82 151 544 607 673 768 837
Size: 82 69 393 63 66 95 69
Fragments arranged by size:
393 95 82 69 69 66 63
SfoI GGC'GCC
Cuts at: 0 206 317 525 783 837
Size: 206 111 208 258 54
Fragments arranged by size:
258 208 206 111 54
SmaI CCC'GGG
Cuts at: 0 200 221 837
Size: 200 21 616
Fragments arranged by size:
616 200 21
XmaI C'CCGG_G
Cuts at: 0 198 219 837
Size: 198 21 618
Fragments arranged by size:
618 198 21
Enzymes that do cut:
AlfI AlwNI ApaI AvaI BanI BanII BbeI BglI
Bme1580I BplI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssHII BstAPI Bsu36I
BtgI BtsI EaeI EagI EarI EciI EcoICRI EcoNI
EcoO109I HaeII HindIII KasI MspA1I NaeI NarI NgoMIV
NotI NruI PasI PfoI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SanDI SfcI SfoI SmaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsgI BsiWI
BsmI BspEI BspHI BspMI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI Eco57MI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HpaI KpnI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI