################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII AccIII AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BbeI BbvCI BfrI BlnI BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FauI FseI FspI HgaI Hin1I HpaI KasI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 1048 GGTACC G^GTACC AccII 1109 CGCG CG^CG Asp718I 1048 GGTACC G^GTACC BanI 1048 GGTACC G^G[CT][GA]CC BanII 688 GAGCCC G[GA]GC[CT]^C BbsI 76 GTCTTC GAAGAC(2/6) BciVI 92 GTATCC GTATCC(6/5) BclI 1661 TGATCA T^GATCA BlpI 685 GCTGAGC GC^T[ACGT]AGC BpmI 1115 CTGGAG CTGGAG(16/14) Bpu1102I 685 GCTGAGC GC^T[ACGT]AGC BpuAI 76 GTCTTC GAAGAC(2/6) BsaI 2310 GGTCTC GGTCTC(1/5) BsaWI 1538 ACCGGA [AT]^CCGG[AT] BsmI 2163 GAATGC GAATGC(1/-1) Bsp1407I 67 TGTACA T^GTACA BsrDI 1493 GCAATG GCAATG(2/0) BsrGI 67 TGTACA T^GTACA BssSI 473 CACGAG CACGAG(-5/-1) BstUI 1109 CGCG CG^CG CelII 685 GCTGAGC GC^T[ACGT]AGC DraI 2337 TTTAAA TTT^AAA DrdI 2302 GACCACGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1677 CTCTTC CTCTTC(1/4) EcoNI 1554 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2019 ATGCAT ATGCA^T FbaI 1661 TGATCA T^GATCA HindIII 844 AAGCTT A^AGCTT KpnI 1048 GGTACC GGTAC^C Ksp632I 1677 CTCTTC CTCTTC(1/4) MfeI 1415 CAATTG C^AATTG MunI 1415 CAATTG C^AATTG MvnI 1109 CGCG CG^CG NcoI 1578 CCATGG C^CATGG NsiI 2019 ATGCAT ATGCA^T PpuMI 2006 GGGACCT [GA]G^G[AT]CC[CT] SapI 1676 GCTCTTC GCTCTTC(1/4) SmaI 2243 CCCGGG CCC^GGG SmlI 2260 CTTGAG C^T[CT][GA]AG SphI 204 GCATGC GCATG^C SspBI 67 TGTACA T^GTACA StuI 807 AGGCCT AGG^CCT TaqI 2230 TCGA T^CGA TthHB8I 2230 TCGA T^CGA XbaI 901 TCTAGA T^CTAGA XmaCI 2243 CCCGGG C^CCGGG XmaI 2243 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1457 GTCTAC GT^[AC][GT]AC 2140 GTAGAC AclI 1271 AACGTT AA^CGTT 2038 AACGTT AcsI 1928 AAATTC [GA]^AATT[CT] 1945 AAATTT AlwNI 540 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 2145 CAGTGCCTG ApoI 1928 AAATTC [GA]^AATT[CT] 1945 AAATTT BalI 1130 TGGCCA TGG^CCA 1307 TGGCCA BbrPI 1670 CACGTG CAC^GTG 2305 CACGTG BcgI 173 GCAAGTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1370 GCACCCGAATCG BglI 751 GCCCGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 943 GCCCTCTGGGC BsgI 1526 CTGCAC GTGCAG(16/14) 482 GTGCAG BspHI 621 TCATGA T^CATGA 2349 TCATGA BspLU11I 114 ACATGT A^CATGT 1754 ACATGT BspMI 701 ACCTGC ACCTGC(4/8) 300 GCAGGT BstXI 1382 CCAAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2064 CCACAAGTATGG DsaI 239 CCGTGG C^C[GA][CT]GG 1578 CCATGG EaeI 1130 TGGCCA [CT]^GGCC[GA] 1307 TGGCCA HaeII 278 GGCGCT [GA]GCGC^[CT] 596 AGCGCC HincII 655 GTCAAC GT[CT]^[GA]AC 1185 GTTGAC HindII 655 GTCAAC GT[CT]^[GA]AC 1185 GTTGAC MluNI 1130 TGGCCA TGG^CCA 1307 TGGCCA MscI 1130 TGGCCA TGG^CCA 1307 TGGCCA PflMI 555 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1167 CCATCATCTGG PmaCI 1670 CACGTG CAC^GTG 2305 CACGTG PmlI 1670 CACGTG CAC^GTG 2305 CACGTG Psp1406I 1271 AACGTT AA^CGTT 2038 AACGTT RcaI 621 TCATGA T^CATGA 2349 TCATGA SbfI 702 CCTGCAGG CCTGCA^GG 1072 CCTGCAGG Sse8387I 702 CCTGCAGG CCTGCA^GG 1072 CCTGCAGG Van91I 555 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1167 CCATCATCTGG XcmI 209 CCAAGAGAATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 896 CCAAGTCTAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AvaI AvaII BbvI BcnI BfaI BglII BmyI BsaAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII