(Circular) (Six-base) MAPSORT of: pFN21AE3304.seq Check: 7585 from: 1 to: 7090 pFN21AE3304 7090 bp With 182 enzymes: SgfI * March 28, 2012 14:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4900 278 Size: 53 83 186 936 3364 2468 Fragments arranged by size: 3364 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3463 4146 4433 1937 Size: 1526 683 287 4594 Fragments arranged by size: 4594 1526 683 287 Acc65I G'GTAC_C Cuts at: 3053 4411 3053 Size: 1358 5732 AclI AA'CG_TT Cuts at: 1563 3277 4044 4389 5217 5590 1563 Size: 1714 767 345 828 373 3063 Fragments arranged by size: 3063 1714 828 767 373 345 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2068 2091 2225 3955 4236 5153 6537 6805 Size: 436 23 134 1730 281 917 1384 268 Cuts at: 6805 1632 Size: 1917 Fragments arranged by size: 1917 1730 1384 917 436 281 268 134 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6893 AflIII A'CryG_T Cuts at: 1203 2119 3361 3759 5989 6966 1203 Size: 916 1242 398 2230 977 1327 Fragments arranged by size: 2230 1327 1242 977 916 398 AgeI A'CCGG_T Cuts at: 5921 5921 Size: 7090 AhdI GACnn_n'nnGTC Cuts at: 5819 5819 Size: 7090 AleI CACnn'nnGTG Cuts at: 1166 2196 1166 Size: 1030 6060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7090 AlwNI CAG_nnn'CTG Cuts at: 2550 4155 4833 6405 2550 Size: 1605 678 1572 3235 Fragments arranged by size: 3235 1605 1572 678 ApaLI G'TGCA_C Cuts at: 5147 6303 5147 Size: 1156 5934 ApoI r'AATT_y Cuts at: 1493 3933 3950 4399 4625 4777 6886 1493 Size: 2440 17 449 226 152 2109 1697 Fragments arranged by size: 2440 2109 1697 449 226 152 17 AseI AT'TA_AT Cuts at: 160 1032 5644 160 Size: 872 4612 1606 Fragments arranged by size: 4612 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 AvaI C'yCGr_G Cuts at: 1870 1943 2253 2431 4248 4415 1870 Size: 73 310 178 1817 167 4545 Fragments arranged by size: 4545 1817 310 178 167 73 BamHI G'GATC_C Cuts at: 4420 4787 4420 Size: 367 6723 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3053 4411 5866 618 Size: 325 217 627 1266 1358 1455 1842 Fragments arranged by size: 1842 1455 1358 1266 627 325 217 BanII G_rGCy'C Cuts at: 1391 2697 4409 1391 Size: 1306 1712 4072 Fragments arranged by size: 4072 1712 1306 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2074 928 Size: 971 175 5944 Fragments arranged by size: 5944 971 175 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2167 2201 3364 3398 5281 5315 1903 Size: 34 230 34 1163 34 1883 34 3678 Fragments arranged by size: 3678 1883 1163 230 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2108 4982 6192 2108 Size: 2874 1210 3006 Fragments arranged by size: 3006 2874 1210 BclI T'GATC_A Cuts at: 1548 3666 1548 Size: 2118 4972 BfrBI ATG'CAT Cuts at: 4026 4026 Size: 7090 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2762 2954 5701 Size: 107 122 71 631 605 1090 192 2747 Cuts at: 5701 136 Size: 1525 Fragments arranged by size: 2747 1525 1090 631 605 192 122 107 71 BglII A'GATC_T Cuts at: 2087 3634 3661 7085 2087 Size: 1547 27 3424 2092 Fragments arranged by size: 3424 2092 1547 27 BlpI GC'TnA_GC Cuts at: 2691 4507 2691 Size: 1816 5274 Bme1580I G_kGCm'C Cuts at: 1790 3018 3741 5151 6307 1790 Size: 1228 723 1410 1156 2573 Fragments arranged by size: 2573 1410 1228 1156 723 BmgBI CAC'GTC Cuts at: 1318 3019 3362 1318 Size: 1701 343 5046 Fragments arranged by size: 5046 1701 343 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2533 4217 4821 Size: 416 53 83 399 24 1116 1684 604 Cuts at: 4821 5779 442 Size: 958 1753 Fragments arranged by size: 1753 1684 1116 958 604 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7090 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3592 3592 Size: 7090 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3141 5750 1239 Size: 1902 2609 2579 Fragments arranged by size: 2609 2579 1902 Bpu10I CC'TnA_GC Cuts at: 3150 3717 3878 3150 Size: 567 161 6362 Fragments arranged by size: 6362 567 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4286 5215 6080 6378 4286 Size: 929 865 298 4998 Fragments arranged by size: 4998 929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4321 5753 882 Size: 617 2822 1432 2219 Fragments arranged by size: 2822 2219 1432 617 BsaAI yAC'GTr Cuts at: 493 1204 3677 4144 4312 4881 493 Size: 711 2473 467 168 569 2702 Fragments arranged by size: 2702 2473 711 569 467 168 BsaBI GATnn'nnATC Cuts at: 7084 7084 Size: 7090 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4897 5279 5914 275 Size: 53 83 186 936 3364 382 635 1451 Fragments arranged by size: 3364 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3543 5523 5921 6195 6342 6753 3543 Size: 1980 398 274 147 411 3880 Fragments arranged by size: 3880 1980 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3842 3872 4203 4233 1178 Size: 30 165 30 2439 30 331 30 4035 Fragments arranged by size: 4035 2439 331 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3254 3255 3331 1186 Size: 2068 1 76 4945 Fragments arranged by size: 4945 2068 76 1 BseYI C'CCAG_C Cuts at: 2686 3687 6293 6789 2686 Size: 1001 2606 496 2987 Fragments arranged by size: 2987 2606 1001 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2508 3516 1163 Size: 573 772 1008 4737 Fragments arranged by size: 4737 1008 772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4540 5301 6329 1540 Size: 275 186 2539 761 1028 2301 Fragments arranged by size: 2539 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5151 5236 6307 5151 Size: 85 1071 5934 Fragments arranged by size: 5934 1071 85 BsmI GAATG_Cn' Cuts at: 4174 4601 4694 4174 Size: 427 93 6570 Fragments arranged by size: 6570 427 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2697 3018 3741 4409 5151 5236 6307 Size: 399 907 321 723 668 742 85 1071 Cuts at: 6307 1391 Size: 2174 Fragments arranged by size: 2174 1071 907 742 723 668 399 321 85 BspEI T'CCGG_A Cuts at: 6753 6753 Size: 7090 BspHI T'CATG_A Cuts at: 2626 4354 4979 6709 2626 Size: 1728 625 1730 3007 Fragments arranged by size: 3007 1730 1728 625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2296 2715 4445 844 Size: 1452 419 1730 3489 Fragments arranged by size: 3489 1730 1452 419 BsrBI CCG'CTC Cuts at: 1394 1823 4977 1394 Size: 429 3154 3507 Fragments arranged by size: 3507 3154 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3505 5585 5759 65 Size: 1183 2257 2080 174 1396 Fragments arranged by size: 2257 2080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5734 5921 1700 Size: 110 3924 187 2869 Fragments arranged by size: 3924 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2072 96 Size: 1976 5114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7090 BssSI C'ACGA_G Cuts at: 2478 5150 6162 2478 Size: 2672 1012 3406 Fragments arranged by size: 3406 2672 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7090 BstBI TT'CG_AA Cuts at: 5894 5894 Size: 7090 BstEII G'GTnAC_C Cuts at: 1184 2248 2347 2569 3793 4193 1184 Size: 1064 99 222 1224 400 4081 Fragments arranged by size: 4081 1224 1064 400 222 99 BstXI CCAn_nnnn'nTGG Cuts at: 3394 4076 3394 Size: 682 6408 BstYI r'GATC_y Cuts at: 1972 2087 2394 3210 3634 3661 3750 3871 4420 Size: 115 307 816 424 27 89 121 549 Cuts at: 4420 4787 5172 5189 6641 6727 6739 7085 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 816 549 424 385 367 346 307 121 115 89 86 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2244 3583 513 Size: 552 1179 1339 4020 Fragments arranged by size: 4020 1339 1179 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6351 Fragments arranged by size: 6351 683 56 BtsI GCAGTG_nn' Cuts at: 2203 2245 4606 5400 5420 2203 Size: 42 2361 794 20 3873 Fragments arranged by size: 3873 2361 794 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7090 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7055 DraI TTT'AAA Cuts at: 4344 4394 4344 Size: 50 7040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4313 6097 809 Size: 507 2997 1784 1802 Fragments arranged by size: 2997 1802 1784 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3135 3312 4537 Size: 54 1425 50 275 18 1305 177 1225 Cuts at: 4537 5426 7055 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1305 1225 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4537 1537 Size: 275 2725 4090 Fragments arranged by size: 4090 2725 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3688 5020 1353 Size: 2335 1332 3423 Fragments arranged by size: 3423 2335 1332 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5677 6051 6197 387 Size: 1064 138 4088 374 146 1280 Fragments arranged by size: 4088 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2091 2225 3141 3955 4236 5153 Size: 393 436 23 134 916 814 281 917 Cuts at: 5153 5750 6537 6805 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 917 916 814 787 597 436 393 281 268 134 23 EcoNI CCTnn'n_nnAGG Cuts at: 3563 3563 Size: 7090 EcoO109I rG'GnC_Cy Cuts at: 2422 3083 3944 4012 2422 Size: 661 861 68 5500 Fragments arranged by size: 5500 861 661 68 EcoRI G'AATT_C Cuts at: 4399 4777 4399 Size: 378 6712 FspI TGC'GCA Cuts at: 5596 5596 Size: 7090 HaeII r_GCGC'y Cuts at: 1678 2287 2605 6237 1678 Size: 609 318 3632 2531 Fragments arranged by size: 3632 2531 609 318 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 2867 2899 5731 Size: 32 135 32 722 32 541 32 2832 Cuts at: 5731 5763 5805 5837 1373 Size: 32 42 32 2626 Fragments arranged by size: 2832 2626 722 541 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2662 3192 4434 4680 669 Size: 988 281 724 530 1242 246 3079 Fragments arranged by size: 3079 1242 988 724 530 281 246 HindIII A'AGCT_T Cuts at: 748 2849 748 Size: 2101 4989 HpaI GTT'AAC Cuts at: 4680 4680 Size: 7090 KpnI G_GTAC'C Cuts at: 3057 4415 3057 Size: 1358 5732 MfeI C'AATT_G Cuts at: 3420 4689 3420 Size: 1269 5821 MluI A'CGCG_T Cuts at: 6966 6966 Size: 7090 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6204 6388 1305 Size: 4899 184 2007 Fragments arranged by size: 4899 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3137 3314 10 Size: 54 1425 343 1305 177 3786 Fragments arranged by size: 3786 1425 1305 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2196 3762 3764 3908 4074 4092 5048 Size: 648 1030 1566 2 144 166 18 956 Cuts at: 5048 5407 5566 518 Size: 359 159 2042 Fragments arranged by size: 2042 1566 1030 956 648 359 166 159 144 18 2 MspA1I CmG'CkG Cuts at: 1606 2025 2446 2492 2690 3691 3833 4124 4506 Size: 419 421 46 198 1001 142 291 382 Cuts at: 4506 5183 6331 6576 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1001 677 421 419 382 291 245 198 142 46 NcoI C'CATG_G Cuts at: 513 1065 3583 513 Size: 552 2518 4020 Fragments arranged by size: 4020 2518 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7090 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7090 NotI GC'GGCC_GC Cuts at: 4537 4537 Size: 7090 NsiI A_TGCA'T Cuts at: 4028 4028 Size: 7090 NspI r_CATG'y Cuts at: 1132 1229 2123 2213 3763 4448 5993 1132 Size: 97 894 90 1550 685 1545 2229 Fragments arranged by size: 2229 1550 1545 894 685 97 90 PasI CC'CwG_GG Cuts at: 1348 2061 2278 1348 Size: 713 217 6160 Fragments arranged by size: 6160 713 217 PciI A'CATG_T Cuts at: 2119 3759 5989 2119 Size: 1640 2230 3220 Fragments arranged by size: 3220 2230 1640 PflMI CCAn_nnn'nTGG Cuts at: 1108 2566 3178 1108 Size: 1458 612 5020 Fragments arranged by size: 5020 1458 612 PfoI T'CCnGG_A Cuts at: 2869 2869 Size: 7090 PmeI GTTT'AAAC Cuts at: 4394 4394 Size: 7090 PmlI CAC'GTG Cuts at: 3677 4312 3677 Size: 635 6455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5159 5191 6672 6704 5159 Size: 32 1481 32 5545 Fragments arranged by size: 5545 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4012 4012 Size: 7090 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7090 PsiI TTA'TAA Cuts at: 4660 4660 Size: 7090 PstI C_TGCA'G Cuts at: 830 2322 2712 3082 4140 4442 830 Size: 1492 390 370 1058 302 3478 Fragments arranged by size: 3478 1492 1058 390 370 302 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7090 PvuII CAG'CTG Cuts at: 2446 2492 2690 3691 3833 2446 Size: 46 198 1001 142 5703 Fragments arranged by size: 5703 1001 198 142 46 SalI G'TCGA_C Cuts at: 1936 4432 1936 Size: 2496 4594 SapI GCTCTTCn'nnn_ Cuts at: 3688 3688 Size: 7090 SbfI CC_TGCA'GG Cuts at: 2712 3082 4442 2712 Size: 370 1360 5360 Fragments arranged by size: 5360 1360 370 ScaI AGT'ACT Cuts at: 5338 7050 5338 Size: 1712 5378 SfcI C'TryA_G Cuts at: 826 1045 2318 2708 3078 4136 4438 5573 6254 Size: 219 1273 390 370 1058 302 1135 681 Cuts at: 6254 6445 826 Size: 191 1471 Fragments arranged by size: 1471 1273 1135 1058 681 390 370 302 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7090 SmaI CCC'GGG Cuts at: 1872 4250 4417 1872 Size: 2378 167 4545 Fragments arranged by size: 4545 2378 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 4265 5194 6095 6357 820 Size: 197 926 2322 929 901 262 1553 Fragments arranged by size: 2322 1553 929 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4881 493 Size: 4388 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7090 SphI G_CATG'C Cuts at: 1132 2213 4448 1132 Size: 1081 2235 3774 Fragments arranged by size: 3774 2235 1081 SspI AAT'ATT Cuts at: 5 52 5014 5 Size: 47 4962 2081 Fragments arranged by size: 4962 2081 47 StuI AGG'CCT Cuts at: 1517 2814 1517 Size: 1297 5793 StyI C'CwwG_G Cuts at: 513 1065 2934 3141 3583 4529 513 Size: 552 1869 207 442 946 3074 Fragments arranged by size: 3074 1869 946 552 442 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2909 3015 3308 5117 5134 5287 5472 Size: 1255 1329 106 293 1809 17 153 185 Cuts at: 5472 5917 325 Size: 445 1498 Fragments arranged by size: 1809 1498 1329 1255 445 293 185 153 106 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2072 2541 4229 Size: 274 80 33 51 1443 95 469 1688 Cuts at: 4229 5336 7048 96 Size: 1107 1712 138 Fragments arranged by size: 1712 1688 1443 1107 469 274 138 95 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4203 4235 7050 7082 4203 Size: 32 2815 32 4211 Fragments arranged by size: 4211 2815 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7090 XbaI T'CTAG_A Cuts at: 2906 4426 2906 Size: 1520 5570 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 2908 2221 Size: 687 6403 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7090 XmaI C'CCGG_G Cuts at: 1870 4248 4415 1870 Size: 2378 167 4545 Fragments arranged by size: 4545 2378 167 XmnI GAAnn'nnTTC Cuts at: 5219 5219 Size: 7090 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4898 276 Size: 53 83 186 936 3364 2468 Fragments arranged by size: 3364 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI