Restriction map for pFN21AE3304
 (Circular) (Six-base) MAPSORT of: pFN21AE3304.seq  Check: 7585  from: 1  to: 7090



pFN21AE3304  7090 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 14:19  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4900     278 
   Size:         53      83     186     936    3364    2468

  Fragments arranged by size: 

                3364    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3463    4146    4433    1937 
   Size:       1526     683     287    4594

  Fragments arranged by size: 

                4594    1526     683     287
 
Acc65I G'GTAC_C

Cuts at:   3053    4411    3053 
   Size:       1358    5732
 
AclI AA'CG_TT

Cuts at:   1563    3277    4044    4389    5217    5590    1563 
   Size:       1714     767     345     828     373    3063

  Fragments arranged by size: 

                3063    1714     828     767     373     345
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2068    2091    2225    3955    4236    5153    6537    6805 
   Size:        436      23     134    1730     281     917    1384     268

Cuts at:   6805    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1730    1384     917     436     281     268     134
                  23
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6893
 
AflIII A'CryG_T

Cuts at:   1203    2119    3361    3759    5989    6966    1203 
   Size:        916    1242     398    2230     977    1327

  Fragments arranged by size: 

                2230    1327    1242     977     916     398
 
AgeI A'CCGG_T

Cuts at:   5921    5921 
   Size:       7090
 
AhdI GACnn_n'nnGTC

Cuts at:   5819    5819 
   Size:       7090
 
AleI CACnn'nnGTG

Cuts at:   1166    2196    1166 
   Size:       1030    6060
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       7090
 
AlwNI CAG_nnn'CTG

Cuts at:   2550    4155    4833    6405    2550 
   Size:       1605     678    1572    3235

  Fragments arranged by size: 

                3235    1605    1572     678
 
ApaLI G'TGCA_C

Cuts at:   5147    6303    5147 
   Size:       1156    5934
 
ApoI r'AATT_y

Cuts at:   1493    3933    3950    4399    4625    4777    6886    1493 
   Size:       2440      17     449     226     152    2109    1697

  Fragments arranged by size: 

                2440    2109    1697     449     226     152      17
 
AseI AT'TA_AT

Cuts at:    160    1032    5644     160 
   Size:        872    4612    1606

  Fragments arranged by size: 

                4612    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7090
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2253    2431    4248    4415    1870 
   Size:         73     310     178    1817     167    4545

  Fragments arranged by size: 

                4545    1817     310     178     167      73
 
BamHI G'GATC_C

Cuts at:   4420    4787    4420 
   Size:        367    6723
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3053    4411    5866     618 
   Size:        325     217     627    1266    1358    1455    1842

  Fragments arranged by size: 

                1842    1455    1358    1266     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2697    4409    1391 
   Size:       1306    1712    4072

  Fragments arranged by size: 

                4072    1712    1306
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2074     928 
   Size:        971     175    5944

  Fragments arranged by size: 

                5944     971     175
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2167    2201    3364    3398    5281    5315    1903 
   Size:         34     230      34    1163      34    1883      34    3678

  Fragments arranged by size: 

                3678    1883    1163     230      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   2108    4982    6192    2108 
   Size:       2874    1210    3006

  Fragments arranged by size: 

                3006    2874    1210
 
BclI T'GATC_A

Cuts at:   1548    3666    1548 
   Size:       2118    4972
 
BfrBI ATG'CAT

Cuts at:   4026    4026 
   Size:       7090
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2762    2954    5701 
   Size:        107     122      71     631     605    1090     192    2747

Cuts at:   5701     136 
   Size:       1525

  Fragments arranged by size: 

                2747    1525    1090     631     605     192     122     107
                  71
 
BglII A'GATC_T

Cuts at:   2087    3634    3661    7085    2087 
   Size:       1547      27    3424    2092

  Fragments arranged by size: 

                3424    2092    1547      27
 
BlpI GC'TnA_GC

Cuts at:   2691    4507    2691 
   Size:       1816    5274
 
Bme1580I G_kGCm'C

Cuts at:   1790    3018    3741    5151    6307    1790 
   Size:       1228     723    1410    1156    2573

  Fragments arranged by size: 

                2573    1410    1228    1156     723
 
BmgBI CAC'GTC

Cuts at:   1318    3019    3362    1318 
   Size:       1701     343    5046

  Fragments arranged by size: 

                5046    1701     343
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2533    4217    4821 
   Size:        416      53      83     399      24    1116    1684     604

Cuts at:   4821    5779     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1684    1116     958     604     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       7090
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3592    3592 
   Size:       7090
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3141    5750    1239 
   Size:       1902    2609    2579

  Fragments arranged by size: 

                2609    2579    1902
 
Bpu10I CC'TnA_GC

Cuts at:   3150    3717    3878    3150 
   Size:        567     161    6362

  Fragments arranged by size: 

                6362     567     161
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4286    5215    6080    6378    4286 
   Size:        929     865     298    4998

  Fragments arranged by size: 

                4998     929     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4321    5753     882 
   Size:        617    2822    1432    2219

  Fragments arranged by size: 

                2822    2219    1432     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3677    4144    4312    4881     493 
   Size:        711    2473     467     168     569    2702

  Fragments arranged by size: 

                2702    2473     711     569     467     168
 
BsaBI GATnn'nnATC

Cuts at:   7084    7084 
   Size:       7090
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4897    5279    5914     275 
   Size:         53      83     186     936    3364     382     635    1451

  Fragments arranged by size: 

                3364    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3543    5523    5921    6195    6342    6753    3543 
   Size:       1980     398     274     147     411    3880

  Fragments arranged by size: 

                3880    1980     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3842    3872    4203    4233    1178 
   Size:         30     165      30    2439      30     331      30    4035

  Fragments arranged by size: 

                4035    2439     331     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3254    3255    3331    1186 
   Size:       2068       1      76    4945

  Fragments arranged by size: 

                4945    2068      76       1
 
BseYI C'CCAG_C

Cuts at:   2686    3687    6293    6789    2686 
   Size:       1001    2606     496    2987

  Fragments arranged by size: 

                2987    2606    1001     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2508    3516    1163 
   Size:        573     772    1008    4737

  Fragments arranged by size: 

                4737    1008     772     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4540    5301    6329    1540 
   Size:        275     186    2539     761    1028    2301

  Fragments arranged by size: 

                2539    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   5151    5236    6307    5151 
   Size:         85    1071    5934

  Fragments arranged by size: 

                5934    1071      85
 
BsmI GAATG_Cn'

Cuts at:   4174    4601    4694    4174 
   Size:        427      93    6570

  Fragments arranged by size: 

                6570     427      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2697    3018    3741    4409    5151    5236    6307 
   Size:        399     907     321     723     668     742      85    1071

Cuts at:   6307    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     907     742     723     668     399     321
                  85
 
BspEI T'CCGG_A

Cuts at:   6753    6753 
   Size:       7090
 
BspHI T'CATG_A

Cuts at:   2626    4354    4979    6709    2626 
   Size:       1728     625    1730    3007

  Fragments arranged by size: 

                3007    1730    1728     625
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2296    2715    4445     844 
   Size:       1452     419    1730    3489

  Fragments arranged by size: 

                3489    1730    1452     419
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4977    1394 
   Size:        429    3154    3507

  Fragments arranged by size: 

                3507    3154     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3505    5585    5759      65 
   Size:       1183    2257    2080     174    1396

  Fragments arranged by size: 

                2257    2080    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5734    5921    1700 
   Size:        110    3924     187    2869

  Fragments arranged by size: 

                3924    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2072      96 
   Size:       1976    5114
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7090
 
BssSI C'ACGA_G

Cuts at:   2478    5150    6162    2478 
   Size:       2672    1012    3406

  Fragments arranged by size: 

                3406    2672    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       7090
 
BstBI TT'CG_AA

Cuts at:   5894    5894 
   Size:       7090
 
BstEII G'GTnAC_C

Cuts at:   1184    2248    2347    2569    3793    4193    1184 
   Size:       1064      99     222    1224     400    4081

  Fragments arranged by size: 

                4081    1224    1064     400     222      99
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3394    4076    3394 
   Size:        682    6408
 
BstYI r'GATC_y

Cuts at:   1972    2087    2394    3210    3634    3661    3750    3871    4420 
   Size:        115     307     816     424      27      89     121     549

Cuts at:   4420    4787    5172    5189    6641    6727    6739    7085    1972 
   Size:        367     385      17    1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452     816     549     424     385     367     346
                 307     121     115      89      86      27      17      12
            
 
BtgI C'CryG_G

Cuts at:    513    1065    2244    3583     513 
   Size:        552    1179    1339    4020

  Fragments arranged by size: 

                4020    1339    1179     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6351

  Fragments arranged by size: 

                6351     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2203    2245    4606    5400    5420    2203 
   Size:         42    2361     794      20    3873

  Fragments arranged by size: 

                3873    2361     794      42      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7090
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    7055
 
DraI TTT'AAA

Cuts at:   4344    4394    4344 
   Size:         50    7040
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4313    6097     809 
   Size:        507    2997    1784    1802

  Fragments arranged by size: 

                2997    1802    1784     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3135    3312    4537 
   Size:         54    1425      50     275      18    1305     177    1225

Cuts at:   4537    5426    7055       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425    1305    1225     889     275     177      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4537    1537 
   Size:        275    2725    4090

  Fragments arranged by size: 

                4090    2725     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3688    5020    1353 
   Size:       2335    1332    3423

  Fragments arranged by size: 

                3423    2335    1332
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5677    6051    6197     387 
   Size:       1064     138    4088     374     146    1280

  Fragments arranged by size: 

                4088    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2068    2091    2225    3141    3955    4236    5153 
   Size:        393     436      23     134     916     814     281     917

Cuts at:   5153    5750    6537    6805    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     917     916     814     787     597     436     393
                 281     268     134      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3563    3563 
   Size:       7090
 
EcoO109I rG'GnC_Cy

Cuts at:   2422    3083    3944    4012    2422 
   Size:        661     861      68    5500

  Fragments arranged by size: 

                5500     861     661      68
 
EcoRI G'AATT_C

Cuts at:   4399    4777    4399 
   Size:        378    6712
 
FspI TGC'GCA

Cuts at:   5596    5596 
   Size:       7090
 
HaeII r_GCGC'y

Cuts at:   1678    2287    2605    6237    1678 
   Size:        609     318    3632    2531

  Fragments arranged by size: 

                3632    2531     609     318
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2294    2326    2867    2899    5731 
   Size:         32     135      32     722      32     541      32    2832

Cuts at:   5731    5763    5805    5837    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2832    2626     722     541     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2662    3192    4434    4680     669 
   Size:        988     281     724     530    1242     246    3079

  Fragments arranged by size: 

                3079    1242     988     724     530     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2849     748 
   Size:       2101    4989
 
HpaI GTT'AAC

Cuts at:   4680    4680 
   Size:       7090
 
KpnI G_GTAC'C

Cuts at:   3057    4415    3057 
   Size:       1358    5732
 
MfeI C'AATT_G

Cuts at:   3420    4689    3420 
   Size:       1269    5821
 
MluI A'CGCG_T

Cuts at:   6966    6966 
   Size:       7090
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    6204    6388    1305 
   Size:       4899     184    2007

  Fragments arranged by size: 

                4899    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3137    3314      10 
   Size:         54    1425     343    1305     177    3786

  Fragments arranged by size: 

                3786    1425    1305     343     177      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2196    3762    3764    3908    4074    4092    5048 
   Size:        648    1030    1566       2     144     166      18     956

Cuts at:   5048    5407    5566     518 
   Size:        359     159    2042

  Fragments arranged by size: 

                2042    1566    1030     956     648     359     166     159
                 144      18       2
 
MspA1I CmG'CkG

Cuts at:   1606    2025    2446    2492    2690    3691    3833    4124    4506 
   Size:        419     421      46     198    1001     142     291     382

Cuts at:   4506    5183    6331    6576    1606 
   Size:        677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1001     677     421     419     382     291
                 245     198     142      46
 
NcoI C'CATG_G

Cuts at:    513    1065    3583     513 
   Size:        552    2518    4020

  Fragments arranged by size: 

                4020    2518     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       7090
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       7090
 
NotI GC'GGCC_GC

Cuts at:   4537    4537 
   Size:       7090
 
NsiI A_TGCA'T

Cuts at:   4028    4028 
   Size:       7090
 
NspI r_CATG'y

Cuts at:   1132    1229    2123    2213    3763    4448    5993    1132 
   Size:         97     894      90    1550     685    1545    2229

  Fragments arranged by size: 

                2229    1550    1545     894     685      97      90
 
PasI CC'CwG_GG

Cuts at:   1348    2061    2278    1348 
   Size:        713     217    6160

  Fragments arranged by size: 

                6160     713     217
 
PciI A'CATG_T

Cuts at:   2119    3759    5989    2119 
   Size:       1640    2230    3220

  Fragments arranged by size: 

                3220    2230    1640
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2566    3178    1108 
   Size:       1458     612    5020

  Fragments arranged by size: 

                5020    1458     612
 
PfoI T'CCnGG_A

Cuts at:   2869    2869 
   Size:       7090
 
PmeI GTTT'AAAC

Cuts at:   4394    4394 
   Size:       7090
 
PmlI CAC'GTG

Cuts at:   3677    4312    3677 
   Size:        635    6455
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5159    5191    6672    6704    5159 
   Size:         32    1481      32    5545

  Fragments arranged by size: 

                5545    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   4012    4012 
   Size:       7090
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7090
 
PsiI TTA'TAA

Cuts at:   4660    4660 
   Size:       7090
 
PstI C_TGCA'G

Cuts at:    830    2322    2712    3082    4140    4442     830 
   Size:       1492     390     370    1058     302    3478

  Fragments arranged by size: 

                3478    1492    1058     390     370     302
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7090
 
PvuII CAG'CTG

Cuts at:   2446    2492    2690    3691    3833    2446 
   Size:         46     198    1001     142    5703

  Fragments arranged by size: 

                5703    1001     198     142      46
 
SalI G'TCGA_C

Cuts at:   1936    4432    1936 
   Size:       2496    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3688    3688 
   Size:       7090
 
SbfI CC_TGCA'GG

Cuts at:   2712    3082    4442    2712 
   Size:        370    1360    5360

  Fragments arranged by size: 

                5360    1360     370
 
ScaI AGT'ACT

Cuts at:   5338    7050    5338 
   Size:       1712    5378
 
SfcI C'TryA_G

Cuts at:    826    1045    2318    2708    3078    4136    4438    5573    6254 
   Size:        219    1273     390     370    1058     302    1135     681

Cuts at:   6254    6445     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1273    1135    1058     681     390     370     302
                 219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7090
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7090
 
SmaI CCC'GGG

Cuts at:   1872    4250    4417    1872 
   Size:       2378     167    4545

  Fragments arranged by size: 

                4545    2378     167
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4265    5194    6095    6357     820 
   Size:        197     926    2322     929     901     262    1553

  Fragments arranged by size: 

                2322    1553     929     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4881     493 
   Size:       4388    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7090
 
SphI G_CATG'C

Cuts at:   1132    2213    4448    1132 
   Size:       1081    2235    3774

  Fragments arranged by size: 

                3774    2235    1081
 
SspI AAT'ATT

Cuts at:      5      52    5014       5 
   Size:         47    4962    2081

  Fragments arranged by size: 

                4962    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2814    1517 
   Size:       1297    5793
 
StyI C'CwwG_G

Cuts at:    513    1065    2934    3141    3583    4529     513 
   Size:        552    1869     207     442     946    3074

  Fragments arranged by size: 

                3074    1869     946     552     442     207
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2909    3015    3308    5117    5134    5287    5472 
   Size:       1255    1329     106     293    1809      17     153     185

Cuts at:   5472    5917     325 
   Size:        445    1498

  Fragments arranged by size: 

                1809    1498    1329    1255     445     293     185     153
                 106      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2072    2541    4229 
   Size:        274      80      33      51    1443      95     469    1688

Cuts at:   4229    5336    7048      96 
   Size:       1107    1712     138

  Fragments arranged by size: 

                1712    1688    1443    1107     469     274     138      95
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4203    4235    7050    7082    4203 
   Size:         32    2815      32    4211

  Fragments arranged by size: 

                4211    2815      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7090
 
XbaI T'CTAG_A

Cuts at:   2906    4426    2906 
   Size:       1520    5570
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2221    2908    2221 
   Size:        687    6403
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7090
 
XmaI C'CCGG_G

Cuts at:   1870    4248    4415    1870 
   Size:       2378     167    4545

  Fragments arranged by size: 

                4545    2378     167
 
XmnI GAAnn'nnTTC

Cuts at:   5219    5219 
   Size:       7090
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4898     276 
   Size:         53      83     186     936    3364    2468

  Fragments arranged by size: 

                3364    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SapI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI
    SexAI     SfiI     SfoI     SrfI     SwaI
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