(Linear) (Six-base) MAPSORT of: KIEE3304.seq Check: 609 from: 1 to: 2385 KIEE3304.orf With 182 enzymes: SgfI * March 22, 2012 16:29 .. AccI GT'mk_AC Cuts at: 0 1458 2141 2385 Size: 1458 683 244 Fragments arranged by size: 1458 683 244 Acc65I G'GTAC_C Cuts at: 0 1048 2385 Size: 1048 1337 AclI AA'CG_TT Cuts at: 0 1272 2039 2385 Size: 1272 767 346 Fragments arranged by size: 1272 767 346 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 86 220 1950 2231 2385 Size: 63 23 134 1730 281 154 Fragments arranged by size: 1730 281 154 134 63 23 AflIII A'CryG_T Cuts at: 0 114 1356 1754 2385 Size: 114 1242 398 631 Fragments arranged by size: 1242 631 398 114 AleI CACnn'nnGTG Cuts at: 0 191 2385 Size: 191 2194 AlwNI CAG_nnn'CTG Cuts at: 0 545 2150 2385 Size: 545 1605 235 Fragments arranged by size: 1605 545 235 ApoI r'AATT_y Cuts at: 0 1928 1945 2385 Size: 1928 17 440 Fragments arranged by size: 1928 440 17 AvaI C'yCGr_G Cuts at: 0 248 426 2243 2385 Size: 248 178 1817 142 Fragments arranged by size: 1817 248 178 142 BanI G'GyrC_C Cuts at: 0 1048 2385 Size: 1048 1337 BanII G_rGCy'C Cuts at: 0 692 2385 Size: 692 1693 BbsI GAAGACnn'nnnn_ Cuts at: 0 69 2385 Size: 69 2316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 162 196 1359 1393 2385 Size: 162 34 1163 34 992 Fragments arranged by size: 1163 992 162 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 103 2385 Size: 103 2282 BclI T'GATC_A Cuts at: 0 1661 2385 Size: 1661 724 BfrBI ATG'CAT Cuts at: 0 2021 2385 Size: 2021 364 BglI GCCn_nnn'nGGC Cuts at: 0 757 949 2385 Size: 757 192 1436 Fragments arranged by size: 1436 757 192 BglII A'GATC_T Cuts at: 0 82 1629 1656 2385 Size: 82 1547 27 729 Fragments arranged by size: 1547 729 82 27 BlpI GC'TnA_GC Cuts at: 0 686 2385 Size: 686 1699 Bme1580I G_kGCm'C Cuts at: 0 1013 1736 2385 Size: 1013 723 649 Fragments arranged by size: 1013 723 649 BmgBI CAC'GTC Cuts at: 0 1014 1357 2385 Size: 1014 343 1028 Fragments arranged by size: 1028 1014 343 BmrI ACTGGGnnnn_n' Cuts at: 0 528 2212 2385 Size: 528 1684 173 Fragments arranged by size: 1684 528 173 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1587 2385 Size: 1587 798 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1136 2385 Size: 1136 1249 Bpu10I CC'TnA_GC Cuts at: 0 1145 1712 1873 2385 Size: 1145 567 161 512 Fragments arranged by size: 1145 567 512 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2281 2385 Size: 2281 104 BsaI GGTCTCn'nnnn_ Cuts at: 0 2316 2385 Size: 2316 69 BsaAI yAC'GTr Cuts at: 0 1672 2139 2307 2385 Size: 1672 467 168 78 Fragments arranged by size: 1672 467 168 78 BsaWI w'CCGG_w Cuts at: 0 1538 2385 Size: 1538 847 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1837 1867 2198 2228 2385 Size: 1837 30 331 30 157 Fragments arranged by size: 1837 331 157 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1249 1250 1326 2385 Size: 1249 1 76 1059 Fragments arranged by size: 1249 1059 76 1 BseYI C'CCAG_C Cuts at: 0 681 1682 2385 Size: 681 1001 703 Fragments arranged by size: 1001 703 681 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 503 1511 2385 Size: 503 1008 874 Fragments arranged by size: 1008 874 503 BsmI GAATG_Cn' Cuts at: 0 2169 2385 Size: 2169 216 Bsp1286I G_dGCh'C Cuts at: 0 692 1013 1736 2385 Size: 692 321 723 649 Fragments arranged by size: 723 692 649 321 BspHI T'CATG_A Cuts at: 0 621 2349 2385 Size: 621 1728 36 Fragments arranged by size: 1728 621 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 291 710 2385 Size: 291 419 1675 Fragments arranged by size: 1675 419 291 BsrDI GCAATG_nn' Cuts at: 0 1500 2385 Size: 1500 885 BsrGI T'GTAC_A Cuts at: 0 67 2385 Size: 67 2318 BssSI C'ACGA_G Cuts at: 0 473 2385 Size: 473 1912 BstEII G'GTnAC_C Cuts at: 0 243 342 564 1788 2188 2385 Size: 243 99 222 1224 400 197 Fragments arranged by size: 1224 400 243 222 197 99 BstXI CCAn_nnnn'nTGG Cuts at: 0 1389 2071 2385 Size: 1389 682 314 Fragments arranged by size: 1389 682 314 BstYI r'GATC_y Cuts at: 0 82 389 1205 1629 1656 1745 1866 2385 Size: 82 307 816 424 27 89 121 519 Fragments arranged by size: 816 519 424 307 121 89 82 27 BtgI C'CryG_G Cuts at: 0 239 1578 2385 Size: 239 1339 807 Fragments arranged by size: 1339 807 239 BtsI GCAGTG_nn' Cuts at: 0 198 240 2385 Size: 198 42 2145 Fragments arranged by size: 2145 198 42 DraI TTT'AAA Cuts at: 0 2339 2385 Size: 2339 46 DrdI GACnn_nn'nnGTC Cuts at: 0 2308 2385 Size: 2308 77 EaeI y'GGCC_r Cuts at: 0 1130 1307 2385 Size: 1130 177 1078 Fragments arranged by size: 1130 1078 177 EarI CTCTTCn'nnn_ Cuts at: 0 1683 2385 Size: 1683 702 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 86 220 1136 1950 2231 2385 Size: 63 23 134 916 814 281 154 Fragments arranged by size: 916 814 281 154 134 63 23 EcoNI CCTnn'n_nnAGG Cuts at: 0 1558 2385 Size: 1558 827 EcoO109I rG'GnC_Cy Cuts at: 0 417 1078 1939 2007 2385 Size: 417 661 861 68 378 Fragments arranged by size: 861 661 417 378 68 HaeII r_GCGC'y Cuts at: 0 282 600 2385 Size: 282 318 1785 Fragments arranged by size: 1785 318 282 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 289 321 862 894 2385 Size: 289 32 541 32 1491 Fragments arranged by size: 1491 541 289 32 32 HincII GTy'rAC Cuts at: 0 657 1187 2385 Size: 657 530 1198 Fragments arranged by size: 1198 657 530 HindIII A'AGCT_T Cuts at: 0 844 2385 Size: 844 1541 KpnI G_GTAC'C Cuts at: 0 1052 2385 Size: 1052 1333 MfeI C'AATT_G Cuts at: 0 1415 2385 Size: 1415 970 MscI TGG'CCA Cuts at: 0 1132 1309 2385 Size: 1132 177 1076 Fragments arranged by size: 1132 1076 177 MslI CAynn'nnrTG Cuts at: 0 191 1757 1759 1903 2069 2087 2385 Size: 191 1566 2 144 166 18 298 Fragments arranged by size: 1566 298 191 166 144 18 2 MspA1I CmG'CkG Cuts at: 0 20 441 487 685 1686 1828 2119 2385 Size: 20 421 46 198 1001 142 291 266 Fragments arranged by size: 1001 421 291 266 198 142 46 20 NcoI C'CATG_G Cuts at: 0 1578 2385 Size: 1578 807 NsiI A_TGCA'T Cuts at: 0 2023 2385 Size: 2023 362 NspI r_CATG'y Cuts at: 0 118 208 1758 2385 Size: 118 90 1550 627 Fragments arranged by size: 1550 627 118 90 PasI CC'CwG_GG Cuts at: 0 56 273 2385 Size: 56 217 2112 Fragments arranged by size: 2112 217 56 PciI A'CATG_T Cuts at: 0 114 1754 2385 Size: 114 1640 631 Fragments arranged by size: 1640 631 114 PflMI CCAn_nnn'nTGG Cuts at: 0 561 1173 2385 Size: 561 612 1212 Fragments arranged by size: 1212 612 561 PfoI T'CCnGG_A Cuts at: 0 864 2385 Size: 864 1521 PmlI CAC'GTG Cuts at: 0 1672 2307 2385 Size: 1672 635 78 Fragments arranged by size: 1672 635 78 PpuMI rG'GwC_Cy Cuts at: 0 2007 2385 Size: 2007 378 PstI C_TGCA'G Cuts at: 0 317 707 1077 2135 2385 Size: 317 390 370 1058 250 Fragments arranged by size: 1058 390 370 317 250 PvuII CAG'CTG Cuts at: 0 441 487 685 1686 1828 2385 Size: 441 46 198 1001 142 557 Fragments arranged by size: 1001 557 441 198 142 46 SapI GCTCTTCn'nnn_ Cuts at: 0 1683 2385 Size: 1683 702 SbfI CC_TGCA'GG Cuts at: 0 707 1077 2385 Size: 707 370 1308 Fragments arranged by size: 1308 707 370 SfcI C'TryA_G Cuts at: 0 313 703 1073 2131 2385 Size: 313 390 370 1058 254 Fragments arranged by size: 1058 390 370 313 254 SmaI CCC'GGG Cuts at: 0 2245 2385 Size: 2245 140 SmlI C'TyrA_G Cuts at: 0 2260 2385 Size: 2260 125 SphI G_CATG'C Cuts at: 0 208 2385 Size: 208 2177 StuI AGG'CCT Cuts at: 0 809 2385 Size: 809 1576 StyI C'CwwG_G Cuts at: 0 929 1136 1578 2385 Size: 929 207 442 807 Fragments arranged by size: 929 807 442 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 904 1010 1303 2385 Size: 904 106 293 1082 Fragments arranged by size: 1082 904 293 106 TatI w'GTAC_w Cuts at: 0 67 536 2224 2385 Size: 67 469 1688 161 Fragments arranged by size: 1688 469 161 67 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2198 2230 2385 Size: 2198 32 155 Fragments arranged by size: 2198 155 32 XbaI T'CTAG_A Cuts at: 0 901 2385 Size: 901 1484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 216 903 2385 Size: 216 687 1482 Fragments arranged by size: 1482 687 216 XmaI C'CCGG_G Cuts at: 0 2243 2385 Size: 2243 142 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AleI AlwNI ApoI AvaI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstEII BstXI BstYI BtgI BtsI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KpnI MfeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PstI PvuII SapI SbfI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BmtI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XhoI XmnI ZraI