Restriction map for
pFN21AE5647
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AfaI AflII AgeI AhdI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI ClaI CpoI DraI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MaeI MfeI
MluI MluNI MroI MscI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PvuI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 984 ACGTGT A^C[GA][CT]GT
Alw44I 1054 GTGCAC G^TGCAC
AocI 778 CCTCAGG CC^T[ACGT]AGG
ApaLI 1054 GTGCAC G^TGCAC
AseI 1161 ATTAAT AT^TAAT
AsnI 1161 ATTAAT AT^TAAT
AvaI 678 CCCGGG C^[CT]CG[GA]G
BamHI 635 GGATCC G^GATCC
BglII 641 AGATCT A^GATCT
BsaBI 636 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1001 ACCGGA [AT]^CCGG[AT]
BsmBI 898 GAGACG CGTCTC(1/5)
BsmFI 892 GGGAC GGGAC(10/14)
BsoBI 678 CCCGGG C^[CT]CG[GA]G
BspHI 383 TCATGA T^CATGA
BsrFI 1321 GCCGGC [GA]^CCGG[CT]
BssSI 1057 CACGAG CACGAG(-5/-1)
Bsu36I 778 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1321 GCCGGC [GA]^CCGG[CT]
DraIII 485 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1319 TGGCCG [CT]^GGCC[GA]
Eco81I 778 CCTCAGG CC^T[ACGT]AGG
EcoNI 453 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 446 CCCGC CCCGC(4/6)
HgaI 387 GACGC GACGC(5/10)
HindIII 855 AAGCTT A^AGCTT
HphI 484 TCACC GGTGA(8/7)
MamI 636 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MwoI 307 GCTGAAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 1321 GCCGGC GCC^GGC
NgoMIV 1321 GCCGGC G^CCGGC
PflMI 109 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1161 ATTAAT AT^TAAT
PstI 869 CTGCAG CTGCA^G
PvuII 1450 CAGCTG CAG^CTG
RcaI 383 TCATGA T^CATGA
SacI 66 GAGCTC GAGCT^C
SfaNI 1045 GATGC GCATC(5/9)
SmaI 678 CCCGGG CCC^GGG
SphI 1013 GCATGC GCATG^C
StuI 479 AGGCCT AGG^CCT
Van91I 109 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 497 CCACTTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 678 CCCGGG C^CCGGG
XmaI 678 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AclI 554 AACGTT AA^CGTT
706 AACGTT
AlwNI 627 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1043 CAGATGCTG
BanII 66 GAGCTC G[GA]GC[CT]^C
1005 GAGCCC
BcnI 678 CCCGG CC^[GC]GG
679 CCGGG
BpmI 1409 CTCCAG CTGGAG(16/14)
57 CTGGAG
BsiYI 109 CCAGTGATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
453 CCTTGTGGAGG
BslI 109 CCAGTGATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
453 CCTTGTGGAGG
BsrDI 344 GCAATG GCAATG(2/0)
524 GCAATG
EcoT14I 476 CCAAGG C^C[AT][AT]GG
1145 CCAAGG
HincII 874 GTTGAC GT[CT]^[GA]AC
1384 GTTGAC
HindII 874 GTTGAC GT[CT]^[GA]AC
1384 GTTGAC
HinfI 4 GATTC G^A[ACGT]TC
757 GAATC
MslI 219 CATAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
232 CATGGATATG
MspA1I 447 CCGCTG C[AC]G^C[GT]G
1450 CAGCTG
NciI 678 CCCGG CC^[GC]GG
679 CCGGG
NspI 1013 GCATGC [GA]CATG^[CT]
1031 ACATGC
Psp1406I 554 AACGTT AA^CGTT
706 AACGTT
SfcI 127 CTACAG C^T[GA][CT]AG
869 CTGCAG
StyI 476 CCAAGG C^C[AT][AT]GG
1145 CCAAGG
TfiI 4 GATTC G^A[AT]TC
757 GAATC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BbvI BmyI BsaJI
BseRI BsiHKAI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII