################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 1054 GTGCAC G^TGCAC AocI 612 CCTCAGG CC^T[ACGT]AGG ApaLI 1054 GTGCAC G^TGCAC AvaI 779 CTCGGG C^[CT]CG[GA]G AvaII 426 GGACC G^G[AT]CC BalI 1227 TGGCCA TGG^CCA BpmI 57 CTGGAG CTGGAG(16/14) BsaWI 1001 ACCGGA [AT]^CCGG[AT] BsgI 1183 CTGCAC GTGCAG(16/14) BsmFI 782 GGGAC GGGAC(10/14) BsoBI 779 CTCGGG C^[CT]CG[GA]G BspHI 383 TCATGA T^CATGA BspMI 1094 ACCTGC ACCTGC(4/8) BsrI 301 CCAGT ACTGG(1/-1) BssSI 1057 CACGAG CACGAG(-5/-1) Bsu36I 612 CCTCAGG CC^T[ACGT]AGG DraIII 485 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 631 CCATGG C^C[GA][CT]GG EaeI 1227 TGGCCA [CT]^GGCC[GA] Eco81I 612 CCTCAGG CC^T[ACGT]AGG EcoNI 453 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1198 GAATTC G^AATTC EcoT22I 1421 ATGCAT ATGCA^T HindIII 855 AAGCTT A^AGCTT HpaI 316 GTTAAC GTT^AAC MluNI 1227 TGGCCA TGG^CCA MscI 1227 TGGCCA TGG^CCA MwoI 1316 GCCTGGCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 631 CCATGG C^CATGG NsiI 1421 ATGCAT ATGCA^T NspI 1031 ACATGC [GA]CATG^[CT] PflMI 1230 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1021 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 127 CTGCAG CTGCA^G PvuII 8 CAGCTG CAG^CTG RcaI 383 TCATGA T^CATGA SfcI 127 CTGCAG C^T[GA][CT]AG Van91I 1230 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 497 CCACTTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AfaI 945 GTAC GT^AC 1446 GTAC AlwNI 302 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1043 CAGATGCTG BamHI 3 GGATCC G^GATCC 635 GGATCC BbvI 449 GCTGC GCAGC(8/12) 1182 GCTGC BclI 264 TGATCA T^GATCA 803 TGATCA BsmAI 898 GAGAC GTCTC(1/5) 29 GTCTC BspDI 554 ATCGAT AT^CGAT 667 ATCGAT Cac8I 445 GCCCGC GC[ACGT]^[ACGT]GC 1321 GCCCGC ClaI 554 ATCGAT AT^CGAT 667 ATCGAT DraII 254 AGGCCCT [GA]G^G[ACGT]CC[CT] 1313 AGGGCCT EcoO109I 254 AGGCCCT [GA]G^G[ACGT]CC[CT] 1313 AGGGCCT EcoT14I 631 CCATGG C^C[AT][AT]GG 1145 CCAAGG FauI 446 CCCGC CCCGC(4/6) 1322 CCCGC FbaI 264 TGATCA T^GATCA 803 TGATCA Fnu4HI 449 GCTGC GC^[ACGT]GC 1182 GCTGC HapII 369 CCGG C^CGG 1002 CCGG HincII 316 GTTAAC GT[CT]^[GA]AC 1384 GTTGAC HindII 316 GTTAAC GT[CT]^[GA]AC 1384 GTTGAC HpaII 369 CCGG C^CGG 1002 CCGG HphI 484 TCACC GGTGA(8/7) 1406 TCACC ItaI 449 GCTGC GC^[ACGT]GC 1182 GCTGC MslI 219 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 232 CATGGATATG MspA1I 8 CAGCTG C[AC]G^C[GT]G 447 CCGCTG MspI 369 CCGG C^CGG 1002 CCGG RsaI 945 GTAC GT^AC 1446 GTAC SacI 66 GAGCTC GAGCT^C 624 GAGCTC SfaNI 1045 GATGC GCATC(5/9) 419 GCATC StyI 631 CCATGG C^C[AT][AT]GG 1145 CCAAGG TseI 449 GCTGC G^C[AT]GC 1182 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI BanII BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrDI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII FokI HaeIII HinfI Ksp632I MaeIII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I XhoII