(Circular) (Six-base) MAPSORT of: pFN21AE4468.seq Check: 9726 from: 1 to: 6175 pFN21AE4468 6175 bp With 182 enzymes: SgfI * March 28, 2012 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 4302 4675 1563 Size: 2739 373 3063 Fragments arranged by size: 3063 2739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 2966 4238 5622 5890 1632 Size: 605 729 1272 1384 268 1917 Fragments arranged by size: 1917 1384 1272 729 605 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6175 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6175 AlwNI CAG_nnn'CTG Cuts at: 2312 3053 3918 5490 2312 Size: 741 865 1572 2997 Fragments arranged by size: 2997 1572 865 741 ApaLI G'TGCA_C Cuts at: 3059 4232 5388 3059 Size: 1173 1156 3846 Fragments arranged by size: 3846 1173 1156 ApoI r'AATT_y Cuts at: 1493 2401 2603 2710 2846 3128 3203 3484 3710 Size: 908 202 107 136 282 75 281 226 Cuts at: 3710 3862 5971 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 908 282 281 226 202 152 136 107 75 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 2784 3500 1870 Size: 73 841 716 4545 Fragments arranged by size: 4545 841 716 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3434 3467 3434 Size: 33 6142 BamHI G'GATC_C Cuts at: 2008 2640 3505 3872 2008 Size: 632 865 367 4311 Fragments arranged by size: 4311 865 632 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3496 4951 618 Size: 325 217 627 1709 1455 1842 Fragments arranged by size: 1842 1709 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2075 2633 3014 3494 1391 Size: 684 558 381 480 4072 Fragments arranged by size: 4072 684 558 480 381 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 5277 4067 Size: 1210 4965 BclI T'GATC_A Cuts at: 1548 2269 2808 1548 Size: 721 539 4915 Fragments arranged by size: 4915 721 539 BfrBI ATG'CAT Cuts at: 3428 3428 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6170 6170 Size: 6175 BlpI GC'TnA_GC Cuts at: 3592 3592 Size: 6175 Bme1580I G_kGCm'C Cuts at: 1790 3063 3158 4236 5392 1790 Size: 1273 95 1078 1156 2573 Fragments arranged by size: 2573 1273 1156 1078 95 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3906 4864 442 Size: 416 53 83 399 24 2489 958 1753 Fragments arranged by size: 2489 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2105 2934 2105 Size: 829 5346 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2083 4835 1239 Size: 844 2752 2579 Fragments arranged by size: 2752 2579 844 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4300 5165 5463 4300 Size: 865 298 5012 Fragments arranged by size: 5012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4838 882 Size: 617 3339 2219 Fragments arranged by size: 3339 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3982 4364 4999 275 Size: 53 83 186 936 2449 382 635 1451 Fragments arranged by size: 2449 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3006 4608 5006 5280 5427 5838 3006 Size: 1602 398 274 147 411 3343 Fragments arranged by size: 3343 1602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2922 2952 3001 3031 1178 Size: 30 165 30 1519 30 49 30 4322 Fragments arranged by size: 4322 1519 165 49 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2097 2294 2434 2480 3077 1186 Size: 911 197 140 46 597 4284 Fragments arranged by size: 4284 911 597 197 140 46 BseYI C'CCAG_C Cuts at: 5378 5874 5378 Size: 496 5679 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3173 1163 Size: 573 1437 4165 Fragments arranged by size: 4165 1437 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 4386 5414 1540 Size: 275 186 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 2075 2524 2633 2785 2915 3063 4236 4321 Size: 44 449 109 152 130 148 1173 85 Cuts at: 4321 5392 2031 Size: 1071 2814 Fragments arranged by size: 2814 1173 1071 449 152 148 130 109 85 44 BsmI GAATG_Cn' Cuts at: 3686 3779 3686 Size: 93 6082 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2075 2524 2633 2785 2915 3014 Size: 399 241 44 449 109 152 130 99 Cuts at: 3014 3063 3158 3494 4236 4321 5392 1391 Size: 49 95 336 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 399 336 241 152 130 109 99 95 85 49 44 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 2388 4064 5794 2388 Size: 1676 1730 2769 Fragments arranged by size: 2769 1730 1676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3108 3530 844 Size: 2264 422 3489 Fragments arranged by size: 3489 2264 422 BsrBI CCG'CTC Cuts at: 1394 1823 4062 1394 Size: 429 2239 3507 Fragments arranged by size: 3507 2239 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2356 2536 3417 4670 4844 65 Size: 1183 1108 180 881 1253 174 1396 Fragments arranged by size: 1396 1253 1183 1108 881 180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4819 5006 1700 Size: 110 3009 187 2869 Fragments arranged by size: 3009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 3062 4235 5247 3062 Size: 1173 1012 3990 Fragments arranged by size: 3990 1173 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6175 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6175 BstYI r'GATC_y Cuts at: 1972 2008 2370 2640 3066 3505 3872 4257 4274 Size: 36 362 270 426 439 367 385 17 Cuts at: 4274 5726 5812 5824 6170 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 439 426 385 367 362 346 270 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2618 2618 Size: 6175 BtgI C'CryG_G Cuts at: 513 1065 2004 2636 513 Size: 552 939 632 4052 Fragments arranged by size: 4052 939 632 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5436 Fragments arranged by size: 5436 683 56 BtsI GCAGTG_nn' Cuts at: 2256 3117 3691 4485 4505 2256 Size: 861 574 794 20 3926 Fragments arranged by size: 3926 861 794 574 20 ClaI AT'CG_AT Cuts at: 1554 2560 2673 1554 Size: 1006 113 5056 Fragments arranged by size: 5056 1006 113 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DraIII CAC_nnn'GTG Cuts at: 2495 2495 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3232 3622 4511 Size: 54 1425 50 275 18 1402 390 889 Cuts at: 4511 6140 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1402 889 390 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2074 2287 2411 2973 4105 1353 Size: 721 213 124 562 1132 3423 Fragments arranged by size: 3423 1132 721 562 213 124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4762 5136 5282 387 Size: 1064 138 3173 374 146 1280 Fragments arranged by size: 3173 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2073 2631 2073 Size: 558 5617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 2237 2966 4238 4835 5622 5890 Size: 393 451 154 729 1272 597 787 268 Cuts at: 5890 1239 Size: 1524 Fragments arranged by size: 1524 1272 787 729 597 451 393 268 154 EcoNI CCTnn'n_nnAGG Cuts at: 2462 2462 Size: 6175 EcoO109I rG'GnC_Cy Cuts at: 2260 3319 2260 Size: 1059 5116 EcoRI G'AATT_C Cuts at: 3203 3484 3862 3203 Size: 281 378 5516 Fragments arranged by size: 5516 378 281 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 5322 1678 Size: 3644 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2322 2354 2916 2922 2948 Size: 32 135 32 750 32 562 6 26 Cuts at: 2948 2954 4816 4848 4890 4922 1373 Size: 6 1862 32 42 32 2626 Fragments arranged by size: 2626 1862 750 562 135 42 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2323 3391 3519 3765 669 Size: 988 281 385 1068 128 246 3079 Fragments arranged by size: 3079 1068 988 385 281 246 128 HindIII A'AGCT_T Cuts at: 748 2860 748 Size: 2112 4063 HpaI GTT'AAC Cuts at: 2323 3765 2323 Size: 1442 4733 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5289 5473 1305 Size: 3984 184 2007 Fragments arranged by size: 3984 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3234 10 Size: 54 1425 343 1402 2951 Fragments arranged by size: 2951 1425 1402 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 2241 4133 4492 4651 518 Size: 648 1062 13 1892 359 159 2042 Fragments arranged by size: 2042 1892 1062 648 359 159 13 MspA1I CmG'CkG Cuts at: 1606 2015 2454 3591 4268 5416 5661 1606 Size: 409 439 1137 677 1148 245 2120 Fragments arranged by size: 2120 1148 1137 677 439 409 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2636 513 Size: 552 939 632 4052 Fragments arranged by size: 4052 939 632 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NsiI A_TGCA'T Cuts at: 3430 3430 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 3040 3533 5078 1132 Size: 97 1811 493 1545 2229 Fragments arranged by size: 2229 1811 1545 493 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 3241 1108 Size: 2133 4042 PfoI T'CCnGG_A Cuts at: 2973 2973 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4244 4276 5757 5789 4244 Size: 32 1481 32 4630 Fragments arranged by size: 4630 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3030 1613 Size: 1417 4758 PsiI TTA'TAA Cuts at: 2571 3745 2571 Size: 1174 5001 PstI C_TGCA'G Cuts at: 830 2136 3527 830 Size: 1306 1391 3478 Fragments arranged by size: 3478 1391 1306 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2015 2015 Size: 6175 SacI G_AGCT'C Cuts at: 2075 2633 2075 Size: 558 5617 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2132 3523 4658 5339 5530 826 Size: 219 1087 1391 1135 681 191 1471 Fragments arranged by size: 1471 1391 1135 1087 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 3502 1872 Size: 1630 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4279 5180 5442 820 Size: 197 926 2336 901 262 1553 Fragments arranged by size: 2336 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6175 StyI C'CwwG_G Cuts at: 513 1065 2004 2636 3150 3614 513 Size: 552 939 632 514 464 3074 Fragments arranged by size: 3074 939 632 552 514 464 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4202 4219 4372 4557 5002 325 Size: 1255 2622 17 153 185 445 1498 Fragments arranged by size: 2622 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4421 6133 96 Size: 274 80 33 51 1443 2444 1712 138 Fragments arranged by size: 2444 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6135 6167 6135 Size: 32 6143 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2509 2509 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 3500 1870 Size: 1630 4545 XmnI GAAnn'nnTTC Cuts at: 4304 4304 Size: 6175 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI