################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII AccI AccIII AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaWI BseAI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRV FseI FspI HgaI HincII HindII HindIII HpaI MluI MluNI MroI MscI MslI NdeI NheI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SmaI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### Acc65I 18 GGTACC G^GTACC AclI 471 AACGTT AA^CGTT AcyI 121 GGCGCC G[GA]^CG[CT]C AocI 61 CCTGAGG CC^T[ACGT]AGG AseI 553 ATTAAT AT^TAAT AsnI 553 ATTAAT AT^TAAT Asp718I 18 GGTACC G^GTACC AvaII 664 GGTCC G^G[AT]CC BanII 1596 GAGCCC G[GA]GC[CT]^C BbeI 121 GGCGCC GGCGC^C BmyI 1596 GAGCCC G[AGT]GC[ACT]^C BsaAI 735 TACGTA [CT]AC^GT[GA] BsaHI 121 GGCGCC G[GA]^CG[CT]C BsaI 1224 GAGACC GGTCTC(1/5) BsgI 236 CTGCAC GTGCAG(16/14) BsmI 570 GAATGC GAATGC(1/-1) Bsp1286I 1596 GAGCCC G[AGT]GC[ACT]^C Bsp1407I 1848 TGTACA T^GTACA BsrFI 124 GCCGGC [GA]^CCGG[CT] BsrGI 1848 TGTACA T^GTACA BstAPI 2007 GCATGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 32 CCACTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 2271 GGATCT [GA]^GATC[CT] Bsu36I 61 CCTGAGG CC^T[ACGT]AGG Cfr10I 124 GCCGGC [GA]^CCGG[CT] DraII 347 AGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 53 CGGCCG [CT]^GGCC[GA] EagI 53 CGGCCG C^GGCCG EclXI 53 CGGCCG C^GGCCG Eco52I 53 CGGCCG C^GGCCG Eco81I 61 CCTGAGG CC^T[ACGT]AGG EcoNI 2149 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 347 AGGCCCC [GA]G^G[ACGT]CC[CT] HaeII 121 GGCGCC [GA]GCGC^[CT] Hin1I 121 GGCGCC G[GA]^CG[CT]C KasI 121 GGCGCC G^GCGCC KpnI 18 GGTACC GGTAC^C KspI 134 CCGCGG CCGC^GG MfeI 844 CAATTG C^AATTG MflI 2271 GGATCT [GA]^GATC[CT] MunI 844 CAATTG C^AATTG NaeI 124 GCCGGC GCC^GGC NarI 121 GGCGCC GG^CGCC NcoI 1689 CCATGG C^CATGG NgoMIV 124 GCCGGC G^CCGGC NotI 52 GCGGCCGC GC^GGCCGC PflMI 1423 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1660 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 553 ATTAAT AT^TAAT Psp1406I 471 AACGTT AA^CGTT PstI 2014 CTGCAG CTGCA^G PvuII 191 CAGCTG CAG^CTG SacII 134 CCGCGG CCGC^GG ScaI 2023 AGTACT AGT^ACT SfoI 121 GGCGCC GGC^GCC SgrAI 123 CGCCGGCG C[GA]^CCGG[CT]G SnaBI 735 TACGTA TAC^GTA SphI 2007 GCATGC GCATG^C SspBI 1848 TGTACA T^GTACA TaqI 1215 TCGA T^CGA Tsp45I 2138 GTGAC ^GT[GC]AC TthHB8I 1215 TCGA T^CGA Van91I 1423 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 32 CCACTCCGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 2271 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 1035 GGATC GGATC(4/5) 2271 GGATC BanI 18 GGTACC G^G[CT][GA]CC 121 GGCGCC BclI 1617 TGATCA T^GATCA 2288 TGATCA BcnI 1304 CCCGG CC^[GC]GG 1801 CCCGG BsaBI 457 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1176 GATAAACATC BsiEI 53 CGGCCG CG[GA][CT]^CG 131 CGACCG BsmAI 1157 GAGAC GTCTC(1/5) 1224 GAGAC BsmFI 1527 GGGAC GGGAC(10/14) 825 GTCCC BspMI 234 ACCTGC ACCTGC(4/8) 2227 ACCTGC BsrDI 1643 GCAATG GCAATG(2/0) 1721 GCAATG BstNI 929 CCTGG CC^[AT]GG 1998 CCTGG EarI 831 GAAGAG CTCTTC(1/4) 1963 GAAGAG EcoRI 1501 GAATTC G^AATTC 1517 GAATTC EcoRII 929 CCTGG ^CC[AT]GG 1998 CCTGG EcoT14I 1553 CCTTGG C^C[AT][AT]GG 1689 CCATGG EcoT22I 326 ATGCAT ATGCA^T 2005 ATGCAT FbaI 1617 TGATCA T^GATCA 2288 TGATCA Ksp632I 831 GAAGAG CTCTTC(1/4) 1963 GAAGAG MamI 457 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1176 GATAAACATC MvaI 929 CCTGG CC^[AT]GG 1998 CCTGG NciI 1304 CCCGG CC^[GC]GG 1801 CCCGG NsiI 326 ATGCAT ATGCA^T 2005 ATGCAT NspI 1433 GCATGT [GA]CATG^[CT] 2007 GCATGC SmlI 602 CTCAAG C^T[CT][GA]AG 2323 CTTGAG StyI 1553 CCTTGG C^C[AT][AT]GG 1689 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI Asp700I BbsI BbvI BfaI BpuAI BsaJI BseRI BsiYI BslI BsrI BssKI BstF5I BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvnI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XmnI