(Circular) (Six-base) MAPSORT of: pFN21AE4223.seq Check: 2050 from: 1 to: 7033 pFN21AE4223 7033 bp With 182 enzymes: SgfI * March 28, 2012 13:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2248 2248 Size: 7033 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4843 278 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4376 1937 Size: 2439 4594 Acc65I G'GTAC_C Cuts at: 2023 4354 2023 Size: 2331 4702 AclI AA'CG_TT Cuts at: 1563 2477 5160 5533 1563 Size: 914 2683 373 3063 Fragments arranged by size: 3063 2683 914 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2925 5096 6480 6748 1632 Size: 1293 2171 1384 268 1917 Fragments arranged by size: 2171 1917 1384 1293 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6836 AflIII A'CryG_T Cuts at: 1203 5932 6909 1203 Size: 4729 977 1327 Fragments arranged by size: 4729 1327 977 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 5762 5762 Size: 7033 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3921 1184 Size: 2737 4296 AlwNI CAG_nnn'CTG Cuts at: 4776 6348 4776 Size: 1572 5461 ApaLI G'TGCA_C Cuts at: 5090 6246 5090 Size: 1156 5877 ApoI r'AATT_y Cuts at: 1493 2169 2567 2909 3417 3506 3522 3773 3819 Size: 676 398 342 508 89 16 251 46 Cuts at: 3819 3978 4302 4309 4342 4568 4720 6829 1493 Size: 159 324 7 33 226 152 2109 1697 Fragments arranged by size: 2109 1697 676 508 398 342 324 251 226 159 152 89 46 33 16 7 AseI AT'TA_AT Cuts at: 160 1032 2559 5587 160 Size: 872 1527 3028 1606 Fragments arranged by size: 3028 1606 1527 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 4358 1870 Size: 73 2415 4545 Fragments arranged by size: 4545 2415 73 BamHI G'GATC_C Cuts at: 4363 4730 4363 Size: 367 6666 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 2126 4354 5809 618 Size: 325 217 627 236 103 2228 1455 1842 Fragments arranged by size: 2228 1842 1455 627 325 236 217 103 BanII G_rGCy'C Cuts at: 1391 3605 4352 1391 Size: 2214 747 4072 Fragments arranged by size: 4072 2214 747 BbeI G_GCGC'C Cuts at: 2130 2130 Size: 7033 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2553 2627 3229 928 Size: 971 654 74 602 4732 Fragments arranged by size: 4732 971 654 602 74 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5224 5258 1903 Size: 34 3287 34 3678 Fragments arranged by size: 3678 3287 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4925 6135 4925 Size: 1210 5823 BclI T'GATC_A Cuts at: 1548 3622 4293 1548 Size: 2074 671 4288 Fragments arranged by size: 4288 2074 671 BfrBI ATG'CAT Cuts at: 2333 4012 2333 Size: 1679 5354 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5644 136 Size: 107 122 71 631 605 3972 1525 Fragments arranged by size: 3972 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7028 7028 Size: 7033 BlpI GC'TnA_GC Cuts at: 4450 4450 Size: 7033 Bme1580I G_kGCm'C Cuts at: 1790 5094 6250 1790 Size: 3304 1156 2573 Fragments arranged by size: 3304 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7033 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2351 4764 5722 Size: 416 53 83 399 24 934 2413 958 Cuts at: 5722 442 Size: 1753 Fragments arranged by size: 2413 1753 958 934 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7033 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4047 4047 Size: 7033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5693 1239 Size: 4454 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2592 4349 5158 6023 6321 2592 Size: 1757 809 865 298 3304 Fragments arranged by size: 3304 1757 865 809 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3223 5696 882 Size: 617 1724 2473 2219 Fragments arranged by size: 2473 2219 1724 617 BsaAI yAC'GTr Cuts at: 493 1204 2742 4824 493 Size: 711 1538 2082 2702 Fragments arranged by size: 2702 2082 1538 711 BsaBI GATnn'nnATC Cuts at: 2466 3185 7027 2466 Size: 719 3842 2472 Fragments arranged by size: 3842 2472 719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2127 4840 5222 5857 Size: 53 83 186 936 594 2713 382 635 Cuts at: 5857 275 Size: 1451 Fragments arranged by size: 2713 1451 936 635 594 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5466 5864 6138 6285 6696 5466 Size: 398 274 147 411 5803 Fragments arranged by size: 5803 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6808 Fragments arranged by size: 6808 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2169 2595 1186 Size: 898 85 426 5624 Fragments arranged by size: 5624 898 426 85 BseYI C'CCAG_C Cuts at: 6236 6732 6236 Size: 496 6537 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2226 1163 Size: 573 490 5970 Fragments arranged by size: 5970 573 490 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2139 4483 5244 6272 1540 Size: 275 186 60 78 2344 761 1028 2301 Fragments arranged by size: 2344 2301 1028 761 275 186 78 60 BsiHKAI G_wGCw'C Cuts at: 5094 5179 6250 5094 Size: 85 1071 5877 Fragments arranged by size: 5877 1071 85 BsmI GAATG_Cn' Cuts at: 2581 4544 4637 2581 Size: 1963 93 4977 Fragments arranged by size: 4977 1963 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3605 4352 5094 5179 6250 1391 Size: 399 1815 747 742 85 1071 2174 Fragments arranged by size: 2174 1815 1071 747 742 399 85 BspEI T'CCGG_A Cuts at: 6696 6696 Size: 7033 BspHI T'CATG_A Cuts at: 4922 6652 4922 Size: 1730 5303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2248 4241 4388 844 Size: 1404 1993 147 3489 Fragments arranged by size: 3489 1993 1404 147 BsrBI CCG'CTC Cuts at: 1394 1823 4920 1394 Size: 429 3097 3507 Fragments arranged by size: 3507 3097 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3655 3733 5528 5702 65 Size: 1183 2407 78 1795 174 1396 Fragments arranged by size: 2407 1795 1396 1183 174 78 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 5677 5864 1700 Size: 110 319 3548 187 2869 Fragments arranged by size: 3548 2869 319 187 110 BsrGI T'GTAC_A Cuts at: 96 3853 96 Size: 3757 3276 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 5093 6105 5093 Size: 1012 6021 BstAPI GCAn_nnn'nTGC Cuts at: 794 4018 794 Size: 3224 3809 BstBI TT'CG_AA Cuts at: 5837 5837 Size: 7033 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7033 BstXI CCAn_nnnn'nTGG Cuts at: 2044 2044 Size: 7033 BstYI r'GATC_y Cuts at: 1972 4276 4363 4730 5115 5132 6584 6670 6682 Size: 2304 87 367 385 17 1452 86 12 Cuts at: 6682 7028 1972 Size: 346 1977 Fragments arranged by size: 2304 1977 1452 385 367 346 87 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2067 2067 Size: 7033 BtgI C'CryG_G Cuts at: 513 1065 2139 3657 3694 513 Size: 552 1074 1518 37 3852 Fragments arranged by size: 3852 1518 1074 552 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3172 487 Size: 683 56 1946 4348 Fragments arranged by size: 4348 1946 683 56 BtsI GCAGTG_nn' Cuts at: 2523 3463 4549 5343 5363 2523 Size: 940 1086 794 20 4193 Fragments arranged by size: 4193 1086 940 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6998 DraI TTT'AAA Cuts at: 2821 3487 3801 4183 4337 2821 Size: 666 314 382 154 5517 Fragments arranged by size: 5517 666 382 314 154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6040 809 Size: 507 4724 1802 Fragments arranged by size: 4724 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 4480 5369 Size: 54 1425 50 275 18 228 2422 889 Cuts at: 5369 6998 8 Size: 1629 43 Fragments arranged by size: 2422 1629 1425 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 4480 1537 Size: 275 246 2422 4090 Fragments arranged by size: 4090 2422 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 2831 3963 4963 1353 Size: 1478 1132 1000 3423 Fragments arranged by size: 3423 1478 1132 1000 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5620 5994 6140 387 Size: 1064 138 4031 374 146 1280 Fragments arranged by size: 4031 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2925 5096 5693 6480 6748 1239 Size: 393 1293 2171 597 787 268 1524 Fragments arranged by size: 2171 1524 1293 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 4158 4158 Size: 7033 EcoO109I rG'GnC_Cy Cuts at: 2353 2353 Size: 7033 EcoRI G'AATT_C Cuts at: 3506 3522 4342 4720 3506 Size: 16 820 378 5819 Fragments arranged by size: 5819 820 378 16 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2705 2705 Size: 7033 FspI TGC'GCA Cuts at: 5539 5539 Size: 7033 HaeII r_GCGC'y Cuts at: 1678 2130 6180 1678 Size: 452 4050 2531 Fragments arranged by size: 4050 2531 452 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 3200 3232 5674 Size: 32 135 32 749 32 847 32 2442 Cuts at: 5674 5706 5748 5780 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2442 847 749 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4377 4623 669 Size: 988 281 2439 246 3079 Fragments arranged by size: 3079 2439 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7033 HpaI GTT'AAC Cuts at: 4623 4623 Size: 7033 KasI G'GCGC_C Cuts at: 2126 2126 Size: 7033 KpnI G_GTAC'C Cuts at: 2027 4358 2027 Size: 2331 4702 MfeI C'AATT_G Cuts at: 2849 4632 2849 Size: 1783 5250 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3499 6147 6331 1305 Size: 2194 2648 184 2007 Fragments arranged by size: 2648 2194 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5211 Fragments arranged by size: 5211 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4991 5350 5509 518 Size: 648 3825 359 159 2042 Fragments arranged by size: 3825 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2141 2198 4449 5126 6274 6519 1606 Size: 438 97 57 2251 677 1148 245 2120 Fragments arranged by size: 2251 2120 1148 677 438 245 97 57 NaeI GCC'GGC Cuts at: 2131 2131 Size: 7033 NarI GG'CG_CC Cuts at: 2127 2127 Size: 7033 NcoI C'CATG_G Cuts at: 513 1065 3694 513 Size: 552 2629 3852 Fragments arranged by size: 3852 2629 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7033 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 7033 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7033 NotI GC'GGCC_GC Cuts at: 2058 4480 2058 Size: 2422 4611 NsiI A_TGCA'T Cuts at: 2335 4014 2335 Size: 1679 5354 NspI r_CATG'y Cuts at: 1132 1229 3442 4016 4391 5936 1132 Size: 97 2213 574 375 1545 2229 Fragments arranged by size: 2229 2213 1545 574 375 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7033 PciI A'CATG_T Cuts at: 5932 5932 Size: 7033 PflMI CCAn_nnn'nTGG Cuts at: 1108 3434 1108 Size: 2326 4707 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5102 5134 6615 6647 5102 Size: 32 1481 32 5488 Fragments arranged by size: 5488 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3669 1613 Size: 2056 4977 PsiI TTA'TAA Cuts at: 3546 4603 3546 Size: 1057 5976 PstI C_TGCA'G Cuts at: 830 4023 4385 830 Size: 3193 362 3478 Fragments arranged by size: 3478 3193 362 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7033 PvuII CAG'CTG Cuts at: 2198 2198 Size: 7033 SacII CC_GC'GG Cuts at: 2142 2142 Size: 7033 SalI G'TCGA_C Cuts at: 1936 4375 1936 Size: 2439 4594 SbfI CC_TGCA'GG Cuts at: 4385 4385 Size: 7033 ScaI AGT'ACT Cuts at: 4030 5281 6993 4030 Size: 1251 1712 4070 Fragments arranged by size: 4070 1712 1251 SfcI C'TryA_G Cuts at: 826 1045 3132 4019 4134 4155 4381 5516 6197 Size: 219 2087 887 115 21 226 1135 681 Cuts at: 6197 6388 826 Size: 191 1471 Fragments arranged by size: 2087 1471 1135 887 681 226 219 191 115 21 SfoI GGC'GCC Cuts at: 2128 2128 Size: 7033 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 2129 1700 Size: 429 6604 SmaI CCC'GGG Cuts at: 1872 4360 1872 Size: 2488 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2607 4328 5137 6038 6300 820 Size: 197 926 664 1721 809 901 262 1553 Fragments arranged by size: 1721 1553 926 901 809 664 262 197 SnaBI TAC'GTA Cuts at: 493 2742 4824 493 Size: 2249 2082 2702 Fragments arranged by size: 2702 2249 2082 SpeI A'CTAG_T Cuts at: 152 152 Size: 7033 SphI G_CATG'C Cuts at: 1132 4016 4391 1132 Size: 2884 375 3774 Fragments arranged by size: 3774 2884 375 SspI AAT'ATT Cuts at: 5 52 4957 5 Size: 47 4905 2081 Fragments arranged by size: 4905 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7033 StyI C'CwwG_G Cuts at: 513 1065 3558 3694 4472 513 Size: 552 2493 136 778 3074 Fragments arranged by size: 3074 2493 778 552 136 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3365 5060 5077 5230 5415 5860 325 Size: 1255 1785 1695 17 153 185 445 1498 Fragments arranged by size: 1785 1695 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2455 3853 4028 Size: 274 80 33 51 1443 478 1398 175 Cuts at: 4028 5279 6991 96 Size: 1251 1712 138 Fragments arranged by size: 1712 1443 1398 1251 478 274 175 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3086 3118 6993 7025 3086 Size: 32 3875 32 3094 Fragments arranged by size: 3875 3094 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7033 XbaI T'CTAG_A Cuts at: 4369 4369 Size: 7033 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2044 2044 Size: 7033 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7033 XmaI C'CCGG_G Cuts at: 1870 4358 1870 Size: 2488 4545 XmnI GAAnn'nnTTC Cuts at: 2373 2840 2910 5162 2373 Size: 467 70 2252 4244 Fragments arranged by size: 4244 2252 467 70 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4841 276 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI