(Linear) (Six-base) MAPSORT of: KIEE4223.seq Check: 6856 from: 1 to: 2328 KIEE4223.orf With 182 enzymes: SgfI * March 22, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 243 2328 Size: 243 2085 Acc65I G'GTAC_C Cuts at: 0 18 2328 Size: 18 2310 AclI AA'CG_TT Cuts at: 0 472 2328 Size: 472 1856 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 920 2328 Size: 920 1408 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1916 2328 Size: 1916 412 ApoI r'AATT_y Cuts at: 0 164 562 904 1412 1501 1517 1768 1814 Size: 164 398 342 508 89 16 251 46 Cuts at: 1814 1973 2297 2304 2328 Size: 159 324 7 24 Fragments arranged by size: 508 398 342 324 251 164 159 89 46 24 16 7 AseI AT'TA_AT Cuts at: 0 554 2328 Size: 554 1774 BanI G'GyrC_C Cuts at: 0 18 121 2328 Size: 18 103 2207 Fragments arranged by size: 2207 103 18 BanII G_rGCy'C Cuts at: 0 1600 2328 Size: 1600 728 BbeI G_GCGC'C Cuts at: 0 125 2328 Size: 125 2203 BbsI GAAGACnn'nnnn_ Cuts at: 0 548 622 1224 2328 Size: 548 74 602 1104 Fragments arranged by size: 1104 602 548 74 BclI T'GATC_A Cuts at: 0 1617 2288 2328 Size: 1617 671 40 Fragments arranged by size: 1617 671 40 BfrBI ATG'CAT Cuts at: 0 328 2007 2328 Size: 328 1679 321 Fragments arranged by size: 1679 328 321 BmrI ACTGGGnnnn_n' Cuts at: 0 346 2328 Size: 346 1982 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2042 2328 Size: 2042 286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 587 2328 Size: 587 1741 BsaI GGTCTCn'nnnn_ Cuts at: 0 1218 2328 Size: 1218 1110 BsaAI yAC'GTr Cuts at: 0 737 2328 Size: 737 1591 BsaBI GATnn'nnATC Cuts at: 0 461 1180 2328 Size: 461 719 1148 Fragments arranged by size: 1148 719 461 BsaHI Gr'CG_yC Cuts at: 0 122 2328 Size: 122 2206 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 79 164 590 2328 Size: 79 85 426 1738 Fragments arranged by size: 1738 426 85 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 221 2328 Size: 221 2107 BsiEI CG_ry'CG Cuts at: 0 56 134 2328 Size: 56 78 2194 Fragments arranged by size: 2194 78 56 BsmI GAATG_Cn' Cuts at: 0 576 2328 Size: 576 1752 Bsp1286I G_dGCh'C Cuts at: 0 1600 2328 Size: 1600 728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 243 2236 2328 Size: 243 1993 92 Fragments arranged by size: 1993 243 92 BsrDI GCAATG_nn' Cuts at: 0 1650 1728 2328 Size: 1650 78 600 Fragments arranged by size: 1650 600 78 BsrFI r'CCGG_y Cuts at: 0 124 2328 Size: 124 2204 BsrGI T'GTAC_A Cuts at: 0 1848 2328 Size: 1848 480 BstAPI GCAn_nnn'nTGC Cuts at: 0 2013 2328 Size: 2013 315 BstXI CCAn_nnnn'nTGG Cuts at: 0 39 2328 Size: 39 2289 BstYI r'GATC_y Cuts at: 0 2271 2328 Size: 2271 57 Bsu36I CC'TnA_GG Cuts at: 0 62 2328 Size: 62 2266 BtgI C'CryG_G Cuts at: 0 134 1652 1689 2328 Size: 134 1518 37 639 Fragments arranged by size: 1518 639 134 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1167 2328 Size: 1167 1161 BtsI GCAGTG_nn' Cuts at: 0 518 1458 2328 Size: 518 940 870 Fragments arranged by size: 940 870 518 DraI TTT'AAA Cuts at: 0 816 1482 1796 2178 2328 Size: 816 666 314 382 150 Fragments arranged by size: 816 666 382 314 150 EaeI y'GGCC_r Cuts at: 0 53 2328 Size: 53 2275 EagI C'GGCC_G Cuts at: 0 53 2328 Size: 53 2275 EarI CTCTTCn'nnn_ Cuts at: 0 826 1958 2328 Size: 826 1132 370 Fragments arranged by size: 1132 826 370 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 920 2328 Size: 920 1408 EcoNI CCTnn'n_nnAGG Cuts at: 0 2153 2328 Size: 2153 175 EcoO109I rG'GnC_Cy Cuts at: 0 348 2328 Size: 348 1980 EcoRI G'AATT_C Cuts at: 0 1501 1517 2328 Size: 1501 16 811 Fragments arranged by size: 1501 811 16 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 700 2328 Size: 700 1628 HaeII r_GCGC'y Cuts at: 0 125 2328 Size: 125 2203 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 316 348 1195 1227 2328 Size: 316 32 847 32 1101 Fragments arranged by size: 1101 847 316 32 32 KasI G'GCGC_C Cuts at: 0 121 2328 Size: 121 2207 KpnI G_GTAC'C Cuts at: 0 22 2328 Size: 22 2306 MfeI C'AATT_G Cuts at: 0 844 2328 Size: 844 1484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1494 2328 Size: 1494 834 MspA1I CmG'CkG Cuts at: 0 39 136 193 2328 Size: 39 97 57 2135 Fragments arranged by size: 2135 97 57 39 NaeI GCC'GGC Cuts at: 0 126 2328 Size: 126 2202 NarI GG'CG_CC Cuts at: 0 122 2328 Size: 122 2206 NcoI C'CATG_G Cuts at: 0 1689 2328 Size: 1689 639 NgoMIV G'CCGG_C Cuts at: 0 124 2328 Size: 124 2204 NotI GC'GGCC_GC Cuts at: 0 53 2328 Size: 53 2275 NsiI A_TGCA'T Cuts at: 0 330 2009 2328 Size: 330 1679 319 Fragments arranged by size: 1679 330 319 NspI r_CATG'y Cuts at: 0 1437 2011 2328 Size: 1437 574 317 Fragments arranged by size: 1437 574 317 PflMI CCAn_nnn'nTGG Cuts at: 0 1429 2328 Size: 1429 899 PshAI GACnn'nnGTC Cuts at: 0 1664 2328 Size: 1664 664 PsiI TTA'TAA Cuts at: 0 1541 2328 Size: 1541 787 PstI C_TGCA'G Cuts at: 0 2018 2328 Size: 2018 310 PvuII CAG'CTG Cuts at: 0 193 2328 Size: 193 2135 SacII CC_GC'GG Cuts at: 0 137 2328 Size: 137 2191 ScaI AGT'ACT Cuts at: 0 2025 2328 Size: 2025 303 SfcI C'TryA_G Cuts at: 0 1127 2014 2129 2150 2328 Size: 1127 887 115 21 178 Fragments arranged by size: 1127 887 178 115 21 SfoI GGC'GCC Cuts at: 0 123 2328 Size: 123 2205 SgrAI Cr'CCGG_yG Cuts at: 0 124 2328 Size: 124 2204 SmlI C'TyrA_G Cuts at: 0 602 2323 2328 Size: 602 1721 5 Fragments arranged by size: 1721 602 5 SnaBI TAC'GTA Cuts at: 0 737 2328 Size: 737 1591 SphI G_CATG'C Cuts at: 0 2011 2328 Size: 2011 317 StyI C'CwwG_G Cuts at: 0 1553 1689 2328 Size: 1553 136 639 Fragments arranged by size: 1553 639 136 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1360 2328 Size: 1360 968 TatI w'GTAC_w Cuts at: 0 450 1848 2023 2328 Size: 450 1398 175 305 Fragments arranged by size: 1398 450 305 175 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1081 1113 2328 Size: 1081 32 1215 Fragments arranged by size: 1215 1081 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 39 2328 Size: 39 2289 XmnI GAAnn'nnTTC Cuts at: 0 368 835 905 2328 Size: 368 467 70 1423 Fragments arranged by size: 1423 467 368 70 Enzymes that do cut: AarI Acc65I AclI AcuI AlfI ApoI AseI BanI BanII BbeI BbsI BclI BfrBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsgI BsiEI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI KpnI MfeI MmeI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI NspI PflMI PshAI PsiI PstI PvuII SacII ScaI SfcI SfoI SgrAI SmlI SnaBI SphI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaWI BsaXI BseYI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraIII DrdI EciI EcoICRI EcoRV FseI FspI FspAI HincII HindIII HpaI MluI MscI MslI NdeI NheI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI SexAI SfiI SgfI SmaI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI