################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AlwNI 1436 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1137 AGCGCT AGC^GCT BamHI 176 GGATCC G^GATCC BbsI 649 GAAGAC GAAGAC(2/6) BfaI 1289 CTAG C^TAG BpuAI 649 GAAGAC GAAGAC(2/6) BseRI 1240 CTCCTC GAGGAG(10/8) BsgI 90 CTGCAC GTGCAG(16/14) BsmBI 771 CGTCTC CGTCTC(1/5) BsmFI 1117 GGGAC GGGAC(10/14) BsmI 1234 GCATTC GAATGC(1/-1) BsrDI 1329 CATTGC GCAATG(2/0) BsrFI 1216 ACCGGC [GA]^CCGG[CT] Bst1107I 1150 GTATAC GTA^TAC BstYI 176 GGATCC [GA]^GATC[CT] BstZ17I 1150 GTATAC GTA^TAC CfoI 1138 GCGC GCG^C Cfr10I 1216 ACCGGC [GA]^CCGG[CT] DraIII 1162 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1137 AGCGCT AGC^GCT EcoNI 575 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 395 CCATGG C^C[AT][AT]GG HaeII 1137 AGCGCT [GA]GCGC^[CT] HaeIII 584 GGCC GG^CC HapII 1217 CCGG C^CGG HhaI 1138 GCGC GCG^C HinP1I 1138 GCGC G^CGC HpaII 1217 CCGG C^CGG HphI 1179 GGTGA GGTGA(8/7) MaeI 1289 CTAG C^TAG MflI 176 GGATCC [GA]^GATC[CT] MslI 914 CATTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1217 CCGG C^CGG NcoI 395 CCATGG C^CATGG PflMI 686 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScaI 407 AGTACT AGT^ACT StyI 395 CCATGG C^C[AT][AT]GG Van91I 686 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 176 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AccI 24 GTCTAC GT^[AC][GT]AC 1150 GTATAC AciI 519 GCGG CCGC(-3/-1) 660 GCGG BsaJI 395 CCATGG C^C[ACGT][ACGT]GG 977 CCGTGG BssSI 315 CACGAG CACGAG(-5/-1) 1036 CTCGTG DsaI 395 CCATGG C^C[GA][CT]GG 977 CCGTGG FauI 519 GCGGG CCCGC(4/6) 660 GCGGG MboII 649 GAAGA GAAGA(8/7) 945 GAAGA MseI 596 TTAA T^TAA 656 TTAA SacI 17 GAGCTC GAGCT^C 303 GAGCTC SfaNI 283 GCATC GCATC(5/9) 327 GCATC Tru9I 596 TTAA T^TAA 656 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AfaI AluI AlwI AspHI AvaII BanI BanII BbvI BmyI BpmI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HinfI ItaI MaeIII MboI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI TfiI TseI Tsp45I Tsp509I TspRI XcmI