(Circular) (Six-base) MAPSORT of: pFN21AE6452.seq Check: 7738 from: 1 to: 6163 pFN21AE6452 6163 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2030 3156 3506 1937 Size: 93 1126 350 4594 Fragments arranged by size: 4594 1126 350 93 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2348 2401 2969 4226 5610 5878 1632 Size: 716 53 568 1257 1384 268 1917 Fragments arranged by size: 1917 1384 1257 716 568 268 53 AfeI AGC'GCT Cuts at: 3144 3144 Size: 6163 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5966 AflIII A'CryG_T Cuts at: 1203 5062 6039 1203 Size: 3859 977 1327 Fragments arranged by size: 3859 1327 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 4892 4892 Size: 6163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2725 3351 1184 Size: 1541 626 3996 Fragments arranged by size: 3996 1541 626 AlwNI CAG_nnn'CTG Cuts at: 3446 3906 5478 3446 Size: 460 1572 4131 Fragments arranged by size: 4131 1572 460 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 3472 3698 3850 5959 1493 Size: 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 3488 1870 Size: 73 1545 4545 Fragments arranged by size: 4545 1545 73 BamHI G'GATC_C Cuts at: 2181 3493 3860 2181 Size: 1312 367 4484 Fragments arranged by size: 4484 1312 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2471 2576 2827 3077 3484 Size: 325 217 627 684 105 251 250 407 Cuts at: 3484 4939 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 684 627 407 325 251 250 217 105 BanII G_rGCy'C Cuts at: 1391 2026 2312 3000 3191 3482 1391 Size: 635 286 688 191 291 4072 Fragments arranged by size: 4072 688 635 291 286 191 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2661 928 Size: 971 762 4430 Fragments arranged by size: 4430 971 762 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4354 4388 1903 Size: 34 2417 34 3678 Fragments arranged by size: 3678 2417 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 1548 Size: 6163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4774 136 Size: 107 122 71 631 605 3102 1525 Fragments arranged by size: 3102 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 3580 3580 Size: 6163 Bme1580I G_kGCm'C Cuts at: 1790 2704 4224 5380 1790 Size: 914 1520 1156 2573 Fragments arranged by size: 2573 1520 1156 914 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3092 3316 3894 Size: 416 53 83 399 24 1675 224 578 Cuts at: 3894 4852 442 Size: 958 1753 Fragments arranged by size: 1753 1675 958 578 416 399 224 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2552 2552 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2025 2373 2604 4823 1239 Size: 786 348 231 2219 2579 Fragments arranged by size: 2579 2219 786 348 231 Bpu10I CC'TnA_GC Cuts at: 2306 3354 2306 Size: 1048 5115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4288 5153 5451 4288 Size: 865 298 5000 Fragments arranged by size: 5000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4826 882 Size: 617 3327 2219 Fragments arranged by size: 3327 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3954 493 Size: 711 2750 2702 Fragments arranged by size: 2750 2702 711 BsaBI GATnn'nnATC Cuts at: 6157 6157 Size: 6163 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3970 4352 4987 275 Size: 53 83 186 936 2437 382 635 1451 Fragments arranged by size: 2437 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4596 4994 5268 5415 5826 4596 Size: 398 274 147 411 4933 Fragments arranged by size: 4933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2023 2053 1178 Size: 30 165 30 620 30 5288 Fragments arranged by size: 5288 620 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3236 1186 Size: 2050 4113 BseYI C'CCAG_C Cuts at: 2052 2272 5366 5862 2052 Size: 220 3094 496 2353 Fragments arranged by size: 3094 2353 496 220 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2080 1163 Size: 573 344 5246 Fragments arranged by size: 5246 573 344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3613 4374 5402 1540 Size: 275 186 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2026 2221 2312 4224 4309 5380 2026 Size: 195 91 1912 85 1071 2809 Fragments arranged by size: 2809 1912 1071 195 91 85 BsmI GAATG_Cn' Cuts at: 3239 3674 3767 3239 Size: 435 93 5635 Fragments arranged by size: 5635 435 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2782 2782 Size: 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2221 2312 2704 3000 3191 3482 Size: 399 236 195 91 392 296 191 291 Cuts at: 3482 4224 4309 5380 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 392 296 291 236 195 191 91 85 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3518 844 Size: 2674 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4050 1394 Size: 429 2227 3507 Fragments arranged by size: 3507 2227 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3333 4658 4832 65 Size: 1183 2085 1325 174 1396 Fragments arranged by size: 2085 1396 1325 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3221 4807 4994 1700 Size: 110 1411 1586 187 2869 Fragments arranged by size: 2869 1586 1411 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6163 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6163 BssSI C'ACGA_G Cuts at: 2320 3041 4223 5235 2320 Size: 721 1182 1012 3248 Fragments arranged by size: 3248 1182 1012 721 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6163 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstYI r'GATC_y Cuts at: 1972 2181 3493 3860 4245 4262 5714 5800 5812 Size: 209 1312 367 385 17 1452 86 12 Cuts at: 5812 6158 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1312 385 367 346 209 86 17 12 BstZ17I GTA'TAC Cuts at: 3157 3157 Size: 6163 BtgI C'CryG_G Cuts at: 513 1065 2004 2400 2982 513 Size: 552 939 396 582 3694 Fragments arranged by size: 3694 939 582 552 396 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3181 487 Size: 683 56 1955 3469 Fragments arranged by size: 3469 1955 683 56 BtsI GCAGTG_nn' Cuts at: 3177 3231 3447 3679 4473 4493 3177 Size: 54 216 232 794 20 4847 Fragments arranged by size: 4847 794 232 216 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6128 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DraIII CAC_nnn'GTG Cuts at: 3172 3172 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3610 4499 6128 Size: 54 1425 50 275 18 1780 889 1629 Cuts at: 6128 8 Size: 43 Fragments arranged by size: 1780 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3610 1537 Size: 275 1798 4090 Fragments arranged by size: 4090 1798 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4093 1353 Size: 2740 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4750 5124 5270 387 Size: 1064 138 3161 374 146 1280 Fragments arranged by size: 3161 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2024 2310 2024 Size: 286 5877 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2025 2348 2373 2401 2604 2969 4226 Size: 393 393 323 25 28 203 365 1257 Cuts at: 4226 4823 5610 5878 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1257 787 597 393 393 365 323 268 203 28 25 EcoNI CCTnn'n_nnAGG Cuts at: 2584 2584 Size: 6163 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FspI TGC'GCA Cuts at: 4669 4669 Size: 6163 HaeII r_GCGC'y Cuts at: 1678 3146 5310 1678 Size: 1468 2164 2531 Fragments arranged by size: 2531 2164 1468 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4804 4836 4878 4910 1373 Size: 32 135 32 3232 32 42 32 2626 Fragments arranged by size: 3232 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3507 3753 669 Size: 988 281 1569 246 3079 Fragments arranged by size: 3079 1569 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6163 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2827 5277 5461 1305 Size: 1522 2450 184 2007 Fragments arranged by size: 2450 2007 1522 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4341 Fragments arranged by size: 4341 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2923 4121 4480 4639 518 Size: 648 1757 1198 359 159 2042 Fragments arranged by size: 2042 1757 1198 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3579 4256 5404 5649 1606 Size: 1973 677 1148 245 2120 Fragments arranged by size: 2120 1973 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2400 513 Size: 552 939 396 4276 Fragments arranged by size: 4276 939 552 396 NdeI CA'TA_TG Cuts at: 387 387 Size: 6163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 3521 5066 1132 Size: 97 2292 1545 2229 Fragments arranged by size: 2292 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6163 PciI A'CATG_T Cuts at: 5062 5062 Size: 6163 PflMI CCAn_nnn'nTGG Cuts at: 1108 2697 1108 Size: 1589 4574 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5745 5777 4232 Size: 32 1481 32 4618 Fragments arranged by size: 4618 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3302 3733 3302 Size: 431 5732 PstI C_TGCA'G Cuts at: 830 2807 3238 3363 3515 830 Size: 1977 431 125 152 3478 Fragments arranged by size: 3478 1977 431 152 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 SacI G_AGCT'C Cuts at: 2026 2312 2026 Size: 286 5877 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SbfI CC_TGCA'GG Cuts at: 3515 3515 Size: 6163 ScaI AGT'ACT Cuts at: 2414 4411 6123 2414 Size: 1997 1712 2454 Fragments arranged by size: 2454 1997 1712 SfcI C'TryA_G Cuts at: 826 1045 2803 3234 3359 3435 3451 3511 4646 Size: 219 1758 431 125 76 16 60 1135 Cuts at: 4646 5327 5518 826 Size: 681 191 1471 Fragments arranged by size: 1758 1471 1135 681 431 219 191 125 76 60 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 3490 1872 Size: 1618 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4267 5168 5430 820 Size: 197 926 2324 901 262 1553 Fragments arranged by size: 2324 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6163 StyI C'CwwG_G Cuts at: 513 1065 2004 2400 3602 513 Size: 552 939 396 1202 3074 Fragments arranged by size: 3074 1202 939 552 396 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2016 2264 2948 3208 4190 4207 4360 Size: 1255 436 248 684 260 982 17 153 Cuts at: 4360 4545 4990 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 982 684 445 436 260 248 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2412 2736 2820 Size: 274 80 33 51 1443 435 324 84 Cuts at: 2820 4409 6121 96 Size: 1589 1712 138 Fragments arranged by size: 1712 1589 1443 435 324 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6123 6155 6123 Size: 32 6131 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6163 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2049 3115 3379 2049 Size: 1066 264 4833 Fragments arranged by size: 4833 1066 264 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6163 XmaI C'CCGG_G Cuts at: 1870 3488 1870 Size: 1618 4545 XmnI GAAnn'nnTTC Cuts at: 4292 4292 Size: 6163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI Bsu36I EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI