(Linear) (Six-base) MAPSORT of: KIEE6452.seq Check: 6670 from: 1 to: 1458 KIEE6452.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AccI GT'mk_AC Cuts at: 0 25 1151 1458 Size: 25 1126 307 Fragments arranged by size: 1126 307 25 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 396 964 1458 Size: 343 53 568 494 Fragments arranged by size: 568 494 343 53 AfeI AGC'GCT Cuts at: 0 1139 1458 Size: 1139 319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 720 1346 1458 Size: 720 626 112 Fragments arranged by size: 720 626 112 AlwNI CAG_nnn'CTG Cuts at: 0 1441 1458 Size: 1441 17 BamHI G'GATC_C Cuts at: 0 176 1458 Size: 176 1282 BanI G'GyrC_C Cuts at: 0 466 571 822 1072 1458 Size: 466 105 251 250 386 Fragments arranged by size: 466 386 251 250 105 BanII G_rGCy'C Cuts at: 0 21 307 995 1186 1458 Size: 21 286 688 191 272 Fragments arranged by size: 688 286 272 191 21 BbsI GAAGACnn'nnnn_ Cuts at: 0 656 1458 Size: 656 802 Bme1580I G_kGCm'C Cuts at: 0 699 1458 Size: 699 759 BmrI ACTGGGnnnn_n' Cuts at: 0 1087 1311 1458 Size: 1087 224 147 Fragments arranged by size: 1087 224 147 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 547 1458 Size: 547 911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 20 368 599 1458 Size: 20 348 231 859 Fragments arranged by size: 859 348 231 20 Bpu10I CC'TnA_GC Cuts at: 0 301 1349 1458 Size: 301 1048 109 Fragments arranged by size: 1048 301 109 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 18 48 1458 Size: 18 30 1410 Fragments arranged by size: 1410 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1231 1458 Size: 1231 227 BseYI C'CCAG_C Cuts at: 0 47 267 1458 Size: 47 220 1191 Fragments arranged by size: 1191 220 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 1458 Size: 75 1383 BsiHKAI G_wGCw'C Cuts at: 0 21 216 307 1458 Size: 21 195 91 1151 Fragments arranged by size: 1151 195 91 21 BsmI GAATG_Cn' Cuts at: 0 1234 1458 Size: 1234 224 BsmBI CGTCTCn'nnnn_ Cuts at: 0 777 1458 Size: 777 681 Bsp1286I G_dGCh'C Cuts at: 0 21 216 307 699 995 1186 1458 Size: 21 195 91 392 296 191 272 Fragments arranged by size: 392 296 272 195 191 91 21 BsrDI GCAATG_nn' Cuts at: 0 1328 1458 Size: 1328 130 BsrFI r'CCGG_y Cuts at: 0 1216 1458 Size: 1216 242 BssSI C'ACGA_G Cuts at: 0 315 1036 1458 Size: 315 721 422 Fragments arranged by size: 721 422 315 BstYI r'GATC_y Cuts at: 0 176 1458 Size: 176 1282 BstZ17I GTA'TAC Cuts at: 0 1152 1458 Size: 1152 306 BtgI C'CryG_G Cuts at: 0 395 977 1458 Size: 395 582 481 Fragments arranged by size: 582 481 395 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1176 1458 Size: 1176 282 BtsI GCAGTG_nn' Cuts at: 0 1172 1226 1442 1458 Size: 1172 54 216 16 Fragments arranged by size: 1172 216 54 16 DraIII CAC_nnn'GTG Cuts at: 0 1167 1458 Size: 1167 291 EcoICRI GAG'CTC Cuts at: 0 19 305 1458 Size: 19 286 1153 Fragments arranged by size: 1153 286 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 20 343 368 396 599 964 1458 Size: 20 323 25 28 203 365 494 Fragments arranged by size: 494 365 323 203 28 25 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 579 1458 Size: 579 879 HaeII r_GCGC'y Cuts at: 0 1141 1458 Size: 1141 317 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 822 1458 Size: 822 636 MslI CAynn'nnrTG Cuts at: 0 918 1458 Size: 918 540 NcoI C'CATG_G Cuts at: 0 395 1458 Size: 395 1063 PflMI CCAn_nnn'nTGG Cuts at: 0 692 1458 Size: 692 766 PsiI TTA'TAA Cuts at: 0 1297 1458 Size: 1297 161 PstI C_TGCA'G Cuts at: 0 802 1233 1358 1458 Size: 802 431 125 100 Fragments arranged by size: 802 431 125 100 SacI G_AGCT'C Cuts at: 0 21 307 1458 Size: 21 286 1151 Fragments arranged by size: 1151 286 21 ScaI AGT'ACT Cuts at: 0 409 1458 Size: 409 1049 SfcI C'TryA_G Cuts at: 0 798 1229 1354 1430 1446 1458 Size: 798 431 125 76 16 12 Fragments arranged by size: 798 431 125 76 16 12 StyI C'CwwG_G Cuts at: 0 395 1458 Size: 395 1063 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 11 259 943 1203 1458 Size: 11 248 684 260 255 Fragments arranged by size: 684 260 255 248 11 TatI w'GTAC_w Cuts at: 0 407 731 815 1458 Size: 407 324 84 643 Fragments arranged by size: 643 407 324 84 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 44 1110 1374 1458 Size: 44 1066 264 84 Fragments arranged by size: 1066 264 84 44 Enzymes that do cut: AccI AcuI AfeI AlfI AlwNI BamHI BanI BanII BbsI Bme1580I BmrI BplI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrFI BssSI BstYI BstZ17I BtgI BtgZI BtsI DraIII EcoICRI Eco57MI EcoNI HaeII MmeI MslI NcoI PflMI PsiI PstI SacI ScaI SfcI StyI TaqII TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI Bsu36I ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI