################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## Acc65I AccII AccIII AciI AflII AflIII AgeI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BamHI BanII BbrPI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HpaI KpnI Ksp632I KspI MaeI MamI MflI MluI MroI MslI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 69 GACGTC GACGT^C AccI 376 GTCTAC GT^[AC][GT]AC AclI 221 AACGTT AA^CGTT AcsI 329 GAATTT [GA]^AATT[CT] AhdI 69 GACGTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 159 GTGCAC G^TGCAC ApaLI 159 GTGCAC G^TGCAC ApoI 329 GAATTT [GA]^AATT[CT] Asp700I 329 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 69 GACGTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 76 GGTCC G^G[AT]CC BalI 903 TGGCCA TGG^CCA BbeI 22 GGCGCC GGCGC^C BbsI 796 GTCTTC GAAGAC(2/6) BcgI 659 GCATACGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 430 GTATCC GTATCC(6/5) BpmI 553 CTGGAG CTGGAG(16/14) BpuAI 796 GTCTTC GAAGAC(2/6) BsaJI 625 CCTTGG C^C[ACGT][ACGT]GG BsaWI 73 TCCGGT [AT]^CCGG[AT] BsmBI 669 CGTCTC CGTCTC(1/5) BsmI 535 GAATGC GAATGC(1/-1) BssSI 724 CTCGTG CACGAG(-5/-1) BstNI 901 CCTGG CC^[AT]GG DraI 1011 TTTAAA TTT^AAA EaeI 903 TGGCCA [CT]^GGCC[GA] Eam1105I 69 GACGTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 274 CCTATTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 901 CCTGG ^CC[AT]GG EcoT14I 625 CCTTGG C^C[AT][AT]GG HaeII 22 GGCGCC [GA]GCGC^[CT] KasI 22 GGCGCC G^GCGCC MfeI 839 CAATTG C^AATTG MluNI 903 TGGCCA TGG^CCA MscI 903 TGGCCA TGG^CCA MunI 839 CAATTG C^AATTG MvaI 901 CCTGG CC^[AT]GG NarI 22 GGCGCC GG^CGCC NlaIII 938 CATG CATG^ NspI 937 GCATGT [GA]CATG^[CT] PflMI 801 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 221 AACGTT AA^CGTT SfoI 22 GGCGCC GGC^GCC SmlI 323 CTCAAG C^T[CT][GA]AG StuI 259 AGGCCT AGG^CCT StyI 625 CCTTGG C^C[AT][AT]GG TaqI 576 TCGA T^CGA TthHB8I 576 TCGA T^CGA Van91I 801 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 329 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 22 GGCGCC G[GA]^CG[CT]C 69 GACGTC AspHI 159 GTGCAC G[AT]GC[AT]^C 176 GTGCTC BanI 22 GGCGCC G^G[CT][GA]CC 318 GGCACC BbvCI 832 CCTCAGC CCTCAGC(-5/-2) 57 GCTGAGG BcnI 26 CCGGG CC^[GC]GG 823 CCGGG BmyI 159 GTGCAC G[AGT]GC[ACT]^C 176 GTGCTC BsaHI 22 GGCGCC G[GA]^CG[CT]C 69 GACGTC BsgI 192 CTGCAC GTGCAG(16/14) 368 GTGCAG BsiHKAI 159 GTGCAC G[AT]GC[AT]^C 176 GTGCTC Bsp1286I 159 GTGCAC G[AGT]GC[ACT]^C 176 GTGCTC BspMI 190 ACCTGC ACCTGC(4/8) 6 GCAGGT Cac8I 136 GCCTGC GC[ACGT]^[ACGT]GC 269 GCTCGC Hin1I 22 GGCGCC G[GA]^CG[CT]C 69 GACGTC HindIII 39 AAGCTT A^AGCTT 1007 AAGCTT MaeII 70 ACGT A^CGT 222 ACGT MspA1I 164 CAGCTG C[AC]G^C[GT]G 805 CAGCTG MwoI 57 GCTGAGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 822 GCCGGGTTTGC NciI 26 CCGGG CC^[GC]GG 823 CCGGG PstI 138 CTGCAG CTGCA^G 499 CTGCAG PvuII 164 CAGCTG CAG^CTG 805 CAGCTG SfaNI 918 GCATC GCATC(5/9) 980 GCATC Tsp45I 394 GTGAC ^GT[GC]AC 512 GTGAC TspRI 157 CAGTG CA[GC]TG[ACGT][ACGT]^ 738 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AfaI AluI AlwNI BbvI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I CfoI Cfr13I DdeI DpnII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspI NdeII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfcI TfiI Tru9I TseI Tsp509I