(Circular) (Six-base) MAPSORT of: pFN21AE6819.seq Check: 1357 from: 1 to: 5731 pFN21AE6819 5731 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2002 2204 2002 Size: 202 5529 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2078 3541 278 Size: 53 83 186 936 542 1463 2468 Fragments arranged by size: 2468 1463 936 542 186 83 53 AccI GT'mk_AC Cuts at: 1937 2382 3074 1937 Size: 445 692 4594 Fragments arranged by size: 4594 692 445 Acc65I G'GTAC_C Cuts at: 3052 3052 Size: 5731 AclI AA'CG_TT Cuts at: 1563 2227 3858 4231 1563 Size: 664 1631 373 3063 Fragments arranged by size: 3063 1631 664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2371 3794 5178 5446 1632 Size: 739 1423 1384 268 1917 Fragments arranged by size: 1917 1423 1384 739 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 4630 5607 1203 Size: 3427 977 1327 Fragments arranged by size: 3427 1327 977 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 2079 4460 2079 Size: 2381 3350 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5731 AlwNI CAG_nnn'CTG Cuts at: 2017 2495 2557 2812 3474 5046 2017 Size: 478 62 255 662 1572 2702 Fragments arranged by size: 2702 1572 662 478 255 62 ApaLI G'TGCA_C Cuts at: 2164 3788 4944 2164 Size: 1624 1156 2951 Fragments arranged by size: 2951 1624 1156 ApoI r'AATT_y Cuts at: 1493 2334 3040 3266 3418 5527 1493 Size: 841 706 226 152 2109 1697 Fragments arranged by size: 2109 1697 841 706 226 152 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 3056 1870 Size: 73 1113 4545 Fragments arranged by size: 4545 1113 73 BamHI G'GATC_C Cuts at: 3061 3428 3061 Size: 367 5364 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2323 3052 4507 618 Size: 325 217 627 240 296 729 1455 1842 Fragments arranged by size: 1842 1455 729 627 325 296 240 217 BanII G_rGCy'C Cuts at: 1391 3050 1391 Size: 1659 4072 BbeI G_GCGC'C Cuts at: 2031 2031 Size: 5731 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2794 928 Size: 971 895 3865 Fragments arranged by size: 3865 971 895 BbvCI CC'TCA_GC Cuts at: 2063 2838 2063 Size: 775 4956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2653 2687 3922 3956 1903 Size: 34 716 34 1235 34 3678 Fragments arranged by size: 3678 1235 716 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2446 3623 4833 2446 Size: 1177 1210 3344 Fragments arranged by size: 3344 1210 1177 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4342 136 Size: 107 122 71 631 605 2670 1525 Fragments arranged by size: 2670 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 3148 3148 Size: 5731 Bme1580I G_kGCm'C Cuts at: 1790 2168 3792 4948 1790 Size: 378 1624 1156 2573 Fragments arranged by size: 2573 1624 1156 378 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3462 4420 442 Size: 416 53 83 399 24 2045 958 1753 Fragments arranged by size: 2045 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 4391 1239 Size: 1340 1812 2579 Fragments arranged by size: 2579 1812 1340 Bpu10I CC'TnA_GC Cuts at: 2063 2457 2838 2063 Size: 394 381 4956 Fragments arranged by size: 4956 394 381 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2313 3856 4721 5019 2313 Size: 1543 865 298 3025 Fragments arranged by size: 3025 1543 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4394 882 Size: 617 2895 2219 Fragments arranged by size: 2895 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3522 493 Size: 711 2318 2702 Fragments arranged by size: 2702 2318 711 BsaBI GATnn'nnATC Cuts at: 5725 5725 Size: 5731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 2075 3538 3920 Size: 53 83 186 936 495 47 1463 382 Cuts at: 3920 4555 275 Size: 635 1451 Fragments arranged by size: 1463 1451 936 635 495 382 186 83 53 47 BsaWI w'CCGG_w Cuts at: 2078 4164 4562 4836 4983 5394 2078 Size: 2086 398 274 147 411 2415 Fragments arranged by size: 2415 2086 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2185 2215 1178 Size: 30 165 30 782 30 4694 Fragments arranged by size: 4694 782 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2080 2193 2566 1186 Size: 894 113 373 4351 Fragments arranged by size: 4351 894 373 113 BseYI C'CCAG_C Cuts at: 2636 4934 5430 2636 Size: 2298 496 2937 Fragments arranged by size: 2937 2298 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2182 2394 1163 Size: 573 446 212 4500 Fragments arranged by size: 4500 573 446 212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3181 3942 4970 1540 Size: 275 186 1180 761 1028 2301 Fragments arranged by size: 2301 1180 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2168 2185 3792 3877 4948 2168 Size: 17 1607 85 1071 2951 Fragments arranged by size: 2951 1607 1071 85 17 BsmI GAATG_Cn' Cuts at: 2546 3242 3335 2546 Size: 696 93 4942 Fragments arranged by size: 4942 696 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2680 2680 Size: 5731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2185 3050 3792 3877 4948 1391 Size: 399 378 17 865 742 85 1071 2174 Fragments arranged by size: 2174 1071 865 742 399 378 85 17 BspEI T'CCGG_A Cuts at: 5394 5394 Size: 5731 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2002 2204 3086 844 Size: 1158 202 882 3489 Fragments arranged by size: 3489 1158 882 202 BsrBI CCG'CTC Cuts at: 1394 1823 3618 1394 Size: 429 1795 3507 Fragments arranged by size: 3507 1795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4226 4400 65 Size: 1183 2978 174 1396 Fragments arranged by size: 2978 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4375 4562 1700 Size: 110 2565 187 2869 Fragments arranged by size: 2869 2565 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5731 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5731 BssSI C'ACGA_G Cuts at: 2729 3791 4803 2729 Size: 1062 1012 3657 Fragments arranged by size: 3657 1062 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5731 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5731 BstYI r'GATC_y Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726 Size: 1089 367 385 17 1452 86 12 346 Cuts at: 5726 1972 Size: 1977 Fragments arranged by size: 1977 1452 1089 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4240 Fragments arranged by size: 4240 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4992 Fragments arranged by size: 4992 683 56 BtsI GCAGTG_nn' Cuts at: 3247 4041 4061 3247 Size: 794 20 4917 Fragments arranged by size: 4917 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3018 3035 3018 Size: 17 5714 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4738 809 Size: 507 3422 1802 Fragments arranged by size: 3422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 3178 4067 Size: 54 1425 50 275 18 1078 270 889 Cuts at: 4067 5696 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1078 889 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3178 1537 Size: 275 1366 4090 Fragments arranged by size: 4090 1366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3661 1353 Size: 2308 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4318 4692 4838 387 Size: 1064 138 2729 374 146 1280 Fragments arranged by size: 2729 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2371 2579 3794 4391 5178 5446 1239 Size: 393 739 208 1215 597 787 268 1524 Fragments arranged by size: 1524 1215 787 739 597 393 268 208 EcoNI CCTnn'n_nnAGG Cuts at: 2283 2283 Size: 5731 EcoRI G'AATT_C Cuts at: 3040 3418 3040 Size: 378 5353 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 5731 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 2031 4878 1678 Size: 353 2847 2531 Fragments arranged by size: 2847 2531 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2065 2097 2908 2940 4372 Size: 32 135 32 493 32 811 32 1432 Cuts at: 4372 4404 4446 4478 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1432 811 493 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 2044 3012 748 Size: 1296 968 3467 Fragments arranged by size: 3467 1296 968 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KasI G'GCGC_C Cuts at: 2027 2027 Size: 5731 KpnI G_GTAC'C Cuts at: 3056 3056 Size: 5731 MfeI C'AATT_G Cuts at: 2844 3330 2844 Size: 486 5245 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2994 4845 5029 1305 Size: 1689 1851 184 2007 Fragments arranged by size: 2007 1851 1689 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2910 10 Size: 54 1425 343 1078 2831 Fragments arranged by size: 2831 1425 1078 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3689 4048 4207 518 Size: 648 2523 359 159 2042 Fragments arranged by size: 2523 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2171 2812 3147 3824 4972 5217 1606 Size: 565 641 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 641 565 335 245 NarI GG'CG_CC Cuts at: 2028 2028 Size: 5731 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4240 Fragments arranged by size: 4240 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 2946 3089 4634 1132 Size: 97 1717 143 1545 2229 Fragments arranged by size: 2229 1717 1545 143 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5731 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 2812 1108 Size: 1704 4027 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PstI C_TGCA'G Cuts at: 830 2147 2508 3083 830 Size: 1317 361 575 3478 Fragments arranged by size: 3478 1317 575 361 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 PvuII CAG'CTG Cuts at: 2171 2812 2171 Size: 641 5090 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 2136 2143 2504 3079 4214 4895 5086 Size: 219 1091 7 361 575 1135 681 191 Cuts at: 5086 826 Size: 1471 Fragments arranged by size: 1471 1135 1091 681 575 361 219 191 7 SfoI GGC'GCC Cuts at: 2029 2029 Size: 5731 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 3058 1872 Size: 1186 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2328 3835 4736 4998 820 Size: 197 926 385 1507 901 262 1553 Fragments arranged by size: 1553 1507 926 901 385 262 197 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 3089 1132 Size: 1957 3774 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 2266 1517 Size: 749 4982 StyI C'CwwG_G Cuts at: 513 1065 2004 2630 3170 513 Size: 552 939 626 540 3074 Fragments arranged by size: 3074 939 626 552 540 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 3758 3775 3928 4113 4558 325 Size: 1255 588 1590 17 153 185 445 1498 Fragments arranged by size: 1590 1498 1255 588 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 2643 3977 Size: 274 80 33 51 1443 210 456 1334 Cuts at: 3977 5689 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1334 456 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2185 2217 2641 2673 5691 5723 2185 Size: 32 424 32 3018 32 2193 Fragments arranged by size: 3018 2193 424 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 3056 1870 Size: 1186 4545 XmnI GAAnn'nnTTC Cuts at: 2338 3860 2338 Size: 1522 4209 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2076 3539 276 Size: 53 83 186 936 542 1463 2468 Fragments arranged by size: 2468 1463 936 542 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI