################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I ClaI CpoI DraI DraII DraIII Eam1105I EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MflI MluI MroI MseI MslI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 724 GACGTC GACGT^C AccI 733 GTCTAC GT^[AC][GT]AC AocI 213 CCTGAGG CC^T[ACGT]AGG Aor51HI 587 AGCGCT AGC^GCT AvaI 145 CCCGAG C^[CT]CG[GA]G AvaII 321 GGACC G^G[AT]CC BalI 281 TGGCCA TGG^CCA BanII 48 GGGCTC G[GA]GC[CT]^C BbeI 64 GGCGCC GGCGC^C BcgI 124 CGAGAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 50 GCTCAGC GC^T[ACGT]AGC BpmI 229 CTGGAG CTGGAG(16/14) Bpu1102I 50 GCTCAGC GC^T[ACGT]AGC BsaI 493 GAGACC GGTCTC(1/5) BsiEI 683 CGGCCG CG[GA][CT]^CG BsmAI 493 GAGAC GTCTC(1/5) BsoBI 145 CCCGAG C^[CT]CG[GA]G BsrBI 180 CCGCTC CCGCTC(-3/-3) BstEII 390 GGTGACC G^GT[ACGT]ACC BstPI 390 GGTGACC G^GT[ACGT]ACC Bsu36I 213 CCTGAGG CC^T[ACGT]AGG CelII 50 GCTCAGC GC^T[ACGT]AGC DrdI 724 GACGTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 683 CGGCCG C^GGCCG EarI 85 CTCTTC CTCTTC(1/4) EclXI 683 CGGCCG C^GGCCG Eco47III 587 AGCGCT AGC^GCT Eco52I 683 CGGCCG C^GGCCG Eco81I 213 CCTGAGG CC^T[ACGT]AGG EcoNI 664 CCTCCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 390 GGTGACC G^GT[ACGT]ACC HgaI 406 GACGC GACGC(5/10) KasI 64 GGCGCC G^GCGCC Ksp632I 85 CTCTTC CTCTTC(1/4) KspI 374 CCGCGG CCGC^GG MaeIII 391 GTGAC ^GT[ACGT]AC MluNI 281 TGGCCA TGG^CCA MscI 281 TGGCCA TGG^CCA NarI 64 GGCGCC GG^CGCC NcoI 109 CCATGG C^CATGG PflMI 273 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 595 CTGCAG CTGCA^G PvuII 598 CAGCTG CAG^CTG SacII 374 CCGCGG CCGC^GG SfcI 595 CTGCAG C^T[GA][CT]AG SfoI 64 GGCGCC GGC^GCC TfiI 247 GATTC G^A[AT]TC Tsp45I 391 GTGAC ^GT[GC]AC Van91I 273 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 308 CCAAGAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 64 GGCGCC G[GA]^CG[CT]C 724 GACGTC AlwNI 130 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 598 CAGCTGCTG AspHI 193 GAGCAC G[AT]GC[AT]^C 457 GAGCAC BsaHI 64 GGCGCC G[GA]^CG[CT]C 724 GACGTC BsiHKAI 193 GAGCAC G[AT]GC[AT]^C 457 GAGCAC BspMI 259 ACCTGC ACCTGC(4/8) 394 ACCTGC BsrI 60 ACTGG ACTGG(1/-1) 79 ACTGG BstAPI 129 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 597 GCAGCTGCTGC DpnII 465 GATC ^GATC 783 GATC Hin1I 64 GGCGCC G[GA]^CG[CT]C 724 GACGTC HphI 390 GGTGA GGTGA(8/7) 767 TCACC MboI 465 GATC ^GATC 783 GATC MboII 86 TCTTC GAAGA(8/7) 171 TCTTC MspA1I 374 CCGCGG C[AC]G^C[GT]G 598 CAGCTG NaeI 119 GCCGGC GCC^GGC 526 GCCGGC NdeII 465 GATC ^GATC 783 GATC NgoMIV 119 GCCGGC G^CCGGC 526 GCCGGC NlaIII 110 CATG CATG^ 186 CATG PleI 764 GACTC GAGTC(4/5) 635 GAGTC Sau3AI 465 GATC ^GATC 783 GATC TaqI 467 TCGA T^CGA 536 TCGA TthHB8I 467 TCGA T^CGA 536 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AluI BanI BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MnlI MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI SfaNI StyI TseI TspRI