(Circular) (Six-base) MAPSORT of: pFN21AE3093.seq Check: 7982 from: 1 to: 5491 pFN21AE3093 5491 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2733 3301 278 Size: 53 83 186 936 1197 568 2468 Fragments arranged by size: 2468 1197 936 568 186 83 53 AccI GT'mk_AC Cuts at: 1937 2739 2834 1937 Size: 802 95 4594 Fragments arranged by size: 4594 802 95 Acc65I G'GTAC_C Cuts at: 2812 2812 Size: 5491 AclI AA'CG_TT Cuts at: 1563 3618 3991 1563 Size: 2055 373 3063 Fragments arranged by size: 3063 2055 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 3554 4938 5206 1632 Size: 872 1050 1384 268 1917 Fragments arranged by size: 1917 1384 1050 872 268 AfeI AGC'GCT Cuts at: 2594 2594 Size: 5491 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5294 AflIII A'CryG_T Cuts at: 1203 4390 5367 1203 Size: 3187 977 1327 Fragments arranged by size: 3187 1327 977 AgeI A'CCGG_T Cuts at: 4322 4322 Size: 5491 AhdI GACnn_n'nnGTC Cuts at: 4220 4220 Size: 5491 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5491 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5491 AlwNI CAG_nnn'CTG Cuts at: 2140 2608 3234 4806 2140 Size: 468 626 1572 2825 Fragments arranged by size: 2825 1572 626 468 ApaLI G'TGCA_C Cuts at: 3548 4704 3548 Size: 1156 4335 ApoI r'AATT_y Cuts at: 1493 2800 3026 3178 5287 1493 Size: 1307 226 152 2109 1697 Fragments arranged by size: 2109 1697 1307 226 152 AseI AT'TA_AT Cuts at: 160 1032 4045 160 Size: 872 3013 1606 Fragments arranged by size: 3013 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5491 AvaI C'yCGr_G Cuts at: 1870 1943 2150 2816 1870 Size: 73 207 666 4545 Fragments arranged by size: 4545 666 207 73 BamHI G'GATC_C Cuts at: 2821 3188 2821 Size: 367 5124 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2069 2106 2333 2534 Size: 325 217 627 230 52 37 227 201 Cuts at: 2534 2812 4267 618 Size: 278 1455 1842 Fragments arranged by size: 1842 1455 627 325 278 230 227 217 201 52 37 BanII G_rGCy'C Cuts at: 1391 2057 2810 1391 Size: 666 753 4072 Fragments arranged by size: 4072 753 666 BbeI G_GCGC'C Cuts at: 2073 2073 Size: 5491 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4520 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2118 2152 3682 3716 1903 Size: 34 181 34 1530 34 3678 Fragments arranged by size: 3678 1530 181 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3383 4593 3383 Size: 1210 4281 BclI T'GATC_A Cuts at: 1548 1548 Size: 5491 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 2294 2318 Size: 107 122 71 631 605 373 249 24 Cuts at: 2318 4102 136 Size: 1784 1525 Fragments arranged by size: 1784 1525 631 605 373 249 122 107 71 24 BglII A'GATC_T Cuts at: 5486 5486 Size: 5491 BlpI GC'TnA_GC Cuts at: 2056 2908 2056 Size: 852 4639 Bme1580I G_kGCm'C Cuts at: 1790 2022 2037 2111 2336 3552 4708 1790 Size: 232 15 74 225 1216 1156 2573 Fragments arranged by size: 2573 1216 1156 232 225 74 15 BmgBI CAC'GTC Cuts at: 1318 2545 2683 1318 Size: 1227 138 4126 Fragments arranged by size: 4126 1227 138 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 3222 4180 Size: 416 53 83 399 24 658 1147 958 Cuts at: 4180 442 Size: 1753 Fragments arranged by size: 1753 1147 958 658 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5491 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2593 2642 2593 Size: 49 5442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 4151 1239 Size: 1016 1896 2579 Fragments arranged by size: 2579 1896 1016 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3616 4481 4779 3616 Size: 865 298 4328 Fragments arranged by size: 4328 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2492 4154 882 Size: 617 993 1662 2219 Fragments arranged by size: 2219 1662 993 617 BsaAI yAC'GTr Cuts at: 493 1204 3282 493 Size: 711 2078 2702 Fragments arranged by size: 2702 2078 711 BsaBI GATnn'nnATC Cuts at: 5485 5485 Size: 5491 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2730 3298 3680 Size: 53 83 186 936 537 660 568 382 Cuts at: 3680 4315 275 Size: 635 1451 Fragments arranged by size: 1451 936 660 635 568 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3924 4322 4596 4743 5154 3924 Size: 398 274 147 411 4261 Fragments arranged by size: 4261 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5266 Fragments arranged by size: 5266 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5491 BseYI C'CCAG_C Cuts at: 2010 2277 2428 4694 5190 2010 Size: 267 151 2266 496 2311 Fragments arranged by size: 2311 2266 496 267 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4918 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2691 2941 3702 4730 1540 Size: 275 186 690 250 761 1028 2301 Fragments arranged by size: 2301 1028 761 690 275 250 186 BsiHKAI G_wGCw'C Cuts at: 2202 2466 3552 3637 4708 2202 Size: 264 1086 85 1071 2985 Fragments arranged by size: 2985 1086 1071 264 85 BsmI GAATG_Cn' Cuts at: 3002 3095 3002 Size: 93 5398 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2037 2057 2111 2202 2336 2466 Size: 399 232 15 20 54 91 134 130 Cuts at: 2466 2810 3552 3637 4708 1391 Size: 344 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 344 232 134 130 91 85 54 20 15 BspEI T'CCGG_A Cuts at: 5154 5154 Size: 5491 BspHI T'CATG_A Cuts at: 3380 5110 3380 Size: 1730 3761 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2273 2408 2846 844 Size: 1429 135 438 3489 Fragments arranged by size: 3489 1429 438 135 BsrBI CCG'CTC Cuts at: 1394 1823 2187 3378 1394 Size: 429 364 1191 3507 Fragments arranged by size: 3507 1191 429 364 BsrDI GCAATG_nn' Cuts at: 65 1248 3986 4160 65 Size: 1183 2738 174 1396 Fragments arranged by size: 2738 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2124 2531 4135 4322 1700 Size: 110 204 110 407 1604 187 2869 Fragments arranged by size: 2869 1604 407 204 187 110 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5491 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5491 BssSI C'ACGA_G Cuts at: 3551 4563 3551 Size: 1012 4479 BstAPI GCAn_nnn'nTGC Cuts at: 794 2140 2608 794 Size: 1346 468 3677 Fragments arranged by size: 3677 1346 468 BstBI TT'CG_AA Cuts at: 4295 4295 Size: 5491 BstEII G'GTnAC_C Cuts at: 1184 2395 1184 Size: 1211 4280 BstYI r'GATC_y Cuts at: 1972 2821 3188 3573 3590 5042 5128 5140 5486 Size: 849 367 385 17 1452 86 12 346 Cuts at: 5486 1972 Size: 1977 Fragments arranged by size: 1977 1452 849 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2219 2219 Size: 5491 BtgI C'CryG_G Cuts at: 513 1065 2114 2379 2550 513 Size: 552 1049 265 171 3454 Fragments arranged by size: 3454 1049 552 265 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2778 487 Size: 683 56 1552 3200 Fragments arranged by size: 3200 1552 683 56 BtsI GCAGTG_nn' Cuts at: 2141 3007 3801 3821 2141 Size: 866 794 20 3811 Fragments arranged by size: 3811 866 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5491 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5456 DraI TTT'AAA Cuts at: 2795 2795 Size: 5491 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2735 4498 809 Size: 507 1419 1763 1802 Fragments arranged by size: 1802 1763 1419 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 2547 2688 Size: 54 1425 50 275 18 456 261 141 Cuts at: 2688 2938 3827 5456 8 Size: 250 889 1629 43 Fragments arranged by size: 1629 1425 889 456 275 261 250 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2688 2938 1537 Size: 275 876 250 4090 Fragments arranged by size: 4090 876 275 250 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 3421 1353 Size: 743 1325 3423 Fragments arranged by size: 3423 1325 743 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2341 2474 2569 4078 4452 4598 Size: 1064 138 752 133 95 1509 374 146 Cuts at: 4598 387 Size: 1280 Fragments arranged by size: 1509 1280 1064 752 374 146 138 133 95 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2504 3554 4151 4938 5206 1239 Size: 393 623 249 1050 597 787 268 1524 Fragments arranged by size: 1524 1050 787 623 597 393 268 249 EcoNI CCTnn'n_nnAGG Cuts at: 2673 2673 Size: 5491 EcoRI G'AATT_C Cuts at: 2800 3178 2800 Size: 378 5113 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2509 2509 Size: 5491 FspI TGC'GCA Cuts at: 3997 3997 Size: 5491 HaeII r_GCGC'y Cuts at: 1678 2073 2210 2324 2375 2596 4638 1678 Size: 395 137 114 51 221 2042 2531 Fragments arranged by size: 2531 2042 395 221 137 114 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4132 4164 4206 4238 1373 Size: 32 135 32 2560 32 42 32 2626 Fragments arranged by size: 2626 2560 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2835 3081 669 Size: 988 281 897 246 3079 Fragments arranged by size: 3079 988 897 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5491 HpaI GTT'AAC Cuts at: 3081 3081 Size: 5491 KasI G'GCGC_C Cuts at: 2069 2069 Size: 5491 KpnI G_GTAC'C Cuts at: 2816 2816 Size: 5491 MfeI C'AATT_G Cuts at: 3090 3090 Size: 5491 MluI A'CGCG_T Cuts at: 5367 5367 Size: 5491 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4605 4789 1305 Size: 3300 184 2007 Fragments arranged by size: 3300 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2288 10 Size: 54 1425 343 456 3213 Fragments arranged by size: 3213 1425 456 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3449 3808 3967 518 Size: 648 2283 359 159 2042 Fragments arranged by size: 2283 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2381 2605 2907 3584 4732 4977 1606 Size: 775 224 302 677 1148 245 2120 Fragments arranged by size: 2120 1148 775 677 302 245 224 NaeI GCC'GGC Cuts at: 2126 2533 2126 Size: 407 5084 NarI GG'CG_CC Cuts at: 2070 2070 Size: 5491 NcoI C'CATG_G Cuts at: 513 1065 2114 513 Size: 552 1049 3890 Fragments arranged by size: 3890 1049 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5491 NgoMIV G'CCGG_C Cuts at: 2124 2531 2124 Size: 407 5084 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5491 NotI GC'GGCC_GC Cuts at: 2938 2938 Size: 5491 NspI r_CATG'y Cuts at: 1132 1229 2849 4394 1132 Size: 97 1620 1545 2229 Fragments arranged by size: 2229 1620 1545 97 PasI CC'CwG_GG Cuts at: 1348 2050 2758 1348 Size: 702 708 4081 Fragments arranged by size: 4081 708 702 PciI A'CATG_T Cuts at: 4390 4390 Size: 5491 PflMI CCAn_nnn'nTGG Cuts at: 1108 2284 1108 Size: 1176 4315 PmeI GTTT'AAAC Cuts at: 2795 2795 Size: 5491 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3560 3592 5073 5105 3560 Size: 32 1481 32 3946 Fragments arranged by size: 3946 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5491 PsiI TTA'TAA Cuts at: 3061 3061 Size: 5491 PstI C_TGCA'G Cuts at: 830 2604 2843 830 Size: 1774 239 3478 Fragments arranged by size: 3478 1774 239 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5491 PvuII CAG'CTG Cuts at: 2605 2605 Size: 5491 SacII CC_GC'GG Cuts at: 2382 2382 Size: 5491 SalI G'TCGA_C Cuts at: 1936 2833 1936 Size: 897 4594 SbfI CC_TGCA'GG Cuts at: 2843 2843 Size: 5491 ScaI AGT'ACT Cuts at: 3739 5451 3739 Size: 1712 3779 SfcI C'TryA_G Cuts at: 826 1045 2600 2839 3974 4655 4846 826 Size: 219 1555 239 1135 681 191 1471 Fragments arranged by size: 1555 1471 1135 681 239 219 191 SfoI GGC'GCC Cuts at: 2071 2071 Size: 5491 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5491 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5491 SmaI CCC'GGG Cuts at: 1872 2818 1872 Size: 946 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3595 4496 4758 820 Size: 197 926 1652 901 262 1553 Fragments arranged by size: 1652 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3282 493 Size: 2789 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5491 SphI G_CATG'C Cuts at: 1132 2849 1132 Size: 1717 3774 SspI AAT'ATT Cuts at: 5 52 3415 5 Size: 47 3363 2081 Fragments arranged by size: 3363 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5491 StyI C'CwwG_G Cuts at: 513 1065 2114 2493 2775 2930 513 Size: 552 1049 379 282 155 3074 Fragments arranged by size: 3074 1049 552 379 282 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3518 3535 3688 3873 4318 325 Size: 1255 1938 17 153 185 445 1498 Fragments arranged by size: 1938 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3737 5449 96 Size: 274 80 33 51 1443 1760 1712 138 Fragments arranged by size: 1760 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5451 5483 5451 Size: 32 5459 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5491 XbaI T'CTAG_A Cuts at: 2827 2827 Size: 5491 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 2320 Size: 5491 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5491 XmaI C'CCGG_G Cuts at: 1870 2816 1870 Size: 946 4545 XmnI GAAnn'nnTTC Cuts at: 3620 3620 Size: 5491 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2731 3299 276 Size: 53 83 186 936 1197 568 2468 Fragments arranged by size: 2468 1197 936 568 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI