Restriction map for
pFN21AE3093
(Linear) (Six-base) MAPSORT of: KIEE3093.seq Check: 1724 from: 1 to: 786
KIEE3093.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AatII G_ACGT'C
Cuts at: 0 728 786
Size: 728 58
AccI GT'mk_AC
Cuts at: 0 734 786
Size: 734 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 786
Size: 499 287
AfeI AGC'GCT
Cuts at: 0 589 786
Size: 589 197
AlwNI CAG_nnn'CTG
Cuts at: 0 135 603 786
Size: 135 468 183
Fragments arranged by size:
468 183 135
AvaI C'yCGr_G
Cuts at: 0 145 786
Size: 145 641
BanI G'GyrC_C
Cuts at: 0 12 64 101 328 529 786
Size: 12 52 37 227 201 257
Fragments arranged by size:
257 227 201 52 37 12
BanII G_rGCy'C
Cuts at: 0 52 786
Size: 52 734
BbeI G_GCGC'C
Cuts at: 0 68 786
Size: 68 718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 113 147 786
Size: 113 34 639
Fragments arranged by size:
639 113 34
BglI GCCn_nnn'nGGC
Cuts at: 0 40 289 313 786
Size: 40 249 24 473
Fragments arranged by size:
473 249 40 24
BlpI GC'TnA_GC
Cuts at: 0 51 786
Size: 51 735
Bme1580I G_kGCm'C
Cuts at: 0 17 32 106 331 786
Size: 17 15 74 225 455
Fragments arranged by size:
455 225 74 17 15
BmgBI CAC'GTC
Cuts at: 0 540 678 786
Size: 540 138 108
Fragments arranged by size:
540 138 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 786
Size: 70 716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 588 637 786
Size: 588 49 149
Fragments arranged by size:
588 149 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 786
Size: 250 536
BsaI GGTCTCn'nnnn_
Cuts at: 0 487 786
Size: 487 299
BsaHI Gr'CG_yC
Cuts at: 0 65 725 786
Size: 65 660 61
Fragments arranged by size:
660 65 61
BseYI C'CCAG_C
Cuts at: 0 5 272 423 786
Size: 5 267 151 363
Fragments arranged by size:
363 267 151 5
BsiEI CG_ry'CG
Cuts at: 0 686 786
Size: 686 100
BsiHKAI G_wGCw'C
Cuts at: 0 197 461 786
Size: 197 264 325
Fragments arranged by size:
325 264 197
Bsp1286I G_dGCh'C
Cuts at: 0 17 32 52 106 197 331 461 786
Size: 17 15 20 54 91 134 130 325
Fragments arranged by size:
325 134 130 91 54 20 17 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 268 403 786
Size: 268 135 383
Fragments arranged by size:
383 268 135
BsrBI CCG'CTC
Cuts at: 0 182 786
Size: 182 604
BsrFI r'CCGG_y
Cuts at: 0 9 119 526 786
Size: 9 110 407 260
Fragments arranged by size:
407 260 110 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 135 603 786
Size: 135 468 183
Fragments arranged by size:
468 183 135
BstEII G'GTnAC_C
Cuts at: 0 390 786
Size: 390 396
Bsu36I CC'TnA_GG
Cuts at: 0 214 786
Size: 214 572
BtgI C'CryG_G
Cuts at: 0 109 374 545 786
Size: 109 265 171 241
Fragments arranged by size:
265 241 171 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 773 786
Size: 773 13
BtsI GCAGTG_nn'
Cuts at: 0 136 786
Size: 136 650
DrdI GACnn_nn'nnGTC
Cuts at: 0 730 786
Size: 730 56
EaeI y'GGCC_r
Cuts at: 0 281 542 683 786
Size: 281 261 141 103
Fragments arranged by size:
281 261 141 103
EagI C'GGCC_G
Cuts at: 0 683 786
Size: 683 103
EarI CTCTTCn'nnn_
Cuts at: 0 91 786
Size: 91 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 336 469 564 786
Size: 336 133 95 222
Fragments arranged by size:
336 222 133 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 499 786
Size: 250 249 287
Fragments arranged by size:
287 250 249
EcoNI CCTnn'n_nnAGG
Cuts at: 0 668 786
Size: 668 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 504 786
Size: 504 282
HaeII r_GCGC'y
Cuts at: 0 68 205 319 370 591 786
Size: 68 137 114 51 221 195
Fragments arranged by size:
221 195 137 114 68 51
KasI G'GCGC_C
Cuts at: 0 64 786
Size: 64 722
MscI TGG'CCA
Cuts at: 0 283 786
Size: 283 503
MspA1I CmG'CkG
Cuts at: 0 376 600 786
Size: 376 224 186
Fragments arranged by size:
376 224 186
NaeI GCC'GGC
Cuts at: 0 121 528 786
Size: 121 407 258
Fragments arranged by size:
407 258 121
NarI GG'CG_CC
Cuts at: 0 65 786
Size: 65 721
NcoI C'CATG_G
Cuts at: 0 109 786
Size: 109 677
NgoMIV G'CCGG_C
Cuts at: 0 119 526 786
Size: 119 407 260
Fragments arranged by size:
407 260 119
PasI CC'CwG_GG
Cuts at: 0 45 753 786
Size: 45 708 33
Fragments arranged by size:
708 45 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 786
Size: 279 507
PstI C_TGCA'G
Cuts at: 0 599 786
Size: 599 187
PvuII CAG'CTG
Cuts at: 0 600 786
Size: 600 186
SacII CC_GC'GG
Cuts at: 0 377 786
Size: 377 409
SfcI C'TryA_G
Cuts at: 0 595 786
Size: 595 191
SfoI GGC'GCC
Cuts at: 0 66 786
Size: 66 720
StyI C'CwwG_G
Cuts at: 0 109 488 770 786
Size: 109 379 282 16
Fragments arranged by size:
379 282 109 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 786
Size: 315 471
ZraI GAC'GTC
Cuts at: 0 726 786
Size: 726 60
Enzymes that do cut:
AatII AccI AcuI AfeI AlwNI AvaI BanI BanII
BbeI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI BsaI BsaHI BseYI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BstAPI BstEII Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI FalI
HaeII KasI MscI MspA1I NaeI NarI NcoI NgoMIV
PasI PflMI PstI PvuII SacII SfcI SfoI StyI
XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BglII BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I ClaI
CspCI DraI DraIII EcoICRI EcoO109I EcoRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MslI NdeI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI