################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbrPI BbsI BbvCI BciVI BclI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BseAI BsgI BsiHKAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DrdI Eam1105I Eco47III Eco81I EcoO109I EcoRI FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MluI MroI NdeI NheI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccII 400 CGCG CG^CG AcsI 177 GAATTT [GA]^AATT[CT] ApoI 177 GAATTT [GA]^AATT[CT] AseI 457 ATTAAT AT^TAAT AsnI 457 ATTAAT AT^TAAT AvaI 44 CTCGGG C^[CT]CG[GA]G BalI 728 TGGCCA TGG^CCA BanI 64 GGCGCC G^G[CT][GA]CC BbeI 64 GGCGCC GGCGC^C BcgI 13 GCAGGTAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 721 GCCTACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 208 GTGCCC G[AGT]GC[ACT]^C BsaAI 475 CACGTA [CT]AC^GT[GA] BsaWI 143 ACCGGA [AT]^CCGG[AT] BsiEI 5 CGGCCG CG[GA][CT]^CG BsiWI 477 CGTACG C^GTACG BsmFI 779 GGGAC GGGAC(10/14) BsoBI 44 CTCGGG C^[CT]CG[GA]G Bsp1286I 208 GTGCCC G[AGT]GC[ACT]^C BspHI 507 TCATGA T^CATGA BspMI 13 GCAGGT ACCTGC(4/8) BsrDI 829 GCAATG GCAATG(2/0) BstAPI 829 GCAATGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 245 GGTTACC G^GT[ACGT]ACC BstPI 245 GGTTACC G^GT[ACGT]ACC BstUI 400 CGCG CG^CG BstYI 624 GGATCT [GA]^GATC[CT] DraIII 564 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 824 CCATGG C^C[GA][CT]GG EagI 5 CGGCCG C^GGCCG EclXI 5 CGGCCG C^GGCCG Eco52I 5 CGGCCG C^GGCCG EcoNI 648 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 245 GGTTACC G^GT[ACGT]ACC EcoRV 255 GATATC GAT^ATC EcoT14I 824 CCATGG C^C[AT][AT]GG EcoT22I 836 ATGCAT ATGCA^T HgaI 113 GCGTC GACGC(5/10) HinfI 316 GACTC G^A[ACGT]TC KasI 64 GGCGCC G^GCGCC MfeI 968 CAATTG C^AATTG MflI 624 GGATCT [GA]^GATC[CT] MluNI 728 TGGCCA TGG^CCA MscI 728 TGGCCA TGG^CCA MspA1I 637 CAGCTG C[AC]G^C[GT]G MunI 968 CAATTG C^AATTG MvnI 400 CGCG CG^CG NarI 64 GGCGCC GG^CGCC NcoI 824 CCATGG C^CATGG NotI 4 GCGGCCGC GC^GGCCGC NsiI 836 ATGCAT ATGCA^T PleI 316 GACTC GAGTC(4/5) PshAI 540 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 457 ATTAAT AT^TAAT PvuII 637 CAGCTG CAG^CTG RcaI 507 TCATGA T^CATGA SexAI 760 ACCTGGT A^CC[AT]GGT SfaNI 964 GATGC GCATC(5/9) SfoI 64 GGCGCC GGC^GCC SgrAI 66 CGCCGGCG C[GA]^CCGG[CT]G SspI 770 AATATT AAT^ATT StyI 824 CCATGG C^C[AT][AT]GG Tsp45I 927 GTCAC ^GT[GC]AC XhoII 624 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 64 GGCGCC G[GA]^CG[CT]C 112 GGCGTC AflII 501 CTTAAG C^TTAAG 991 CTTAAG BcnI 275 CCCGG CC^[GC]GG 752 CCCGG BfaI 619 CTAG C^TAG 940 CTAG BfrI 501 CTTAAG C^TTAAG 991 CTTAAG BsaHI 64 GGCGCC G[GA]^CG[CT]C 112 GGCGTC BsaI 494 GAGACC GGTCTC(1/5) 1004 GAGACC BsrI 585 CCAGT ACTGG(1/-1) 793 CCAGT BstF5I 272 CATCC GGATG(2/0) 963 GGATG CfoI 65 GCGC GCG^C 82 GCGC EaeI 5 CGGCCG [CT]^GGCC[GA] 728 TGGCCA EarI 349 GAAGAG CTCTTC(1/4) 687 GAAGAG FauI 59 GCGGG CCCGC(4/6) 77 GCGGG FokI 272 CATCC GGATG(9/13) 963 GGATG HaeII 64 GGCGCC [GA]GCGC^[CT] 81 GGCGCT HhaI 65 GCGC GCG^C 82 GCGC Hin1I 64 GGCGCC G[GA]^CG[CT]C 112 GGCGTC HinP1I 65 GCGC G^CGC 82 GCGC HphI 1042 GGTGA GGTGA(8/7) 385 TCACC Ksp632I 349 GAAGAG CTCTTC(1/4) 687 GAAGAG MaeI 619 CTAG C^TAG 940 CTAG MaeII 476 ACGT A^CGT 930 ACGT MboII 349 GAAGA GAAGA(8/7) 687 GAAGA NaeI 67 GCCGGC GCC^GGC 91 GCCGGC NciI 275 CCCGG CC^[GC]GG 752 CCCGG NgoMIV 67 GCCGGC G^CCGGC 91 GCCGGC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BsaJI BseRI BsiYI BslI BsmAI BsrFI BssKI BstNI Cac8I Cfr10I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HpaII ItaI MaeIII MboI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SmlI Tru9I TseI Tsp509I TspRI