(Circular) (Six-base) MAPSORT of: pFN21AE5437.seq Check: 4597 from: 1 to: 5758 pFN21AE5437 5758 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3821 5205 5473 1632 Size: 2189 1384 268 1917 Fragments arranged by size: 2189 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2506 2996 820 Size: 197 1489 490 3582 Fragments arranged by size: 3582 1489 490 197 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 2210 2705 1166 Size: 1044 495 4219 Fragments arranged by size: 4219 1044 495 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2228 1184 Size: 1044 4714 AlwNI CAG_nnn'CTG Cuts at: 3501 5073 3501 Size: 1572 4186 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 2182 3067 3293 3445 5554 1493 Size: 689 885 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 689 226 152 AseI AT'TA_AT Cuts at: 160 1032 2463 4312 160 Size: 872 1431 1849 1606 Fragments arranged by size: 1849 1606 1431 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2049 3083 1870 Size: 73 106 1034 4545 Fragments arranged by size: 4545 1034 106 73 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 3079 4534 618 Size: 325 217 627 282 1010 1455 1842 Fragments arranged by size: 1842 1455 1010 627 325 282 217 BanII G_rGCy'C Cuts at: 1391 3077 1391 Size: 1686 4072 BbeI G_GCGC'C Cuts at: 2073 2073 Size: 5758 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4787 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2007 2041 3949 3983 1903 Size: 34 70 34 1908 34 3678 Fragments arranged by size: 3678 1908 70 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BfrBI ATG'CAT Cuts at: 2843 2843 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2732 4369 Size: 107 122 71 631 605 337 723 1637 Cuts at: 4369 136 Size: 1525 Fragments arranged by size: 1637 1525 723 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2217 3819 4975 1790 Size: 427 1602 1156 2573 Fragments arranged by size: 2573 1602 1156 427 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 4447 442 Size: 416 53 83 399 24 2072 958 1753 Fragments arranged by size: 2072 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4418 1239 Size: 3179 2579 Bpu10I CC'TnA_GC Cuts at: 2979 2979 Size: 5758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2291 2948 3883 4748 5046 2291 Size: 657 935 865 298 3003 Fragments arranged by size: 3003 935 865 657 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2493 3003 4421 882 Size: 617 994 510 1418 2219 Fragments arranged by size: 2219 1418 994 617 510 BsaAI yAC'GTr Cuts at: 493 1204 2482 3549 493 Size: 711 1278 1067 2702 Fragments arranged by size: 2702 1278 1067 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2118 3565 3947 Size: 53 83 186 936 537 48 1447 382 Cuts at: 3947 4582 275 Size: 635 1451 Fragments arranged by size: 1451 1447 936 635 537 382 186 83 53 48 BsaWI w'CCGG_w Cuts at: 2148 4191 4589 4863 5010 5421 2148 Size: 2043 398 274 147 411 2485 Fragments arranged by size: 2485 2043 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2035 2423 2426 1186 Size: 844 5 388 3 4518 Fragments arranged by size: 4518 844 388 5 3 BseYI C'CCAG_C Cuts at: 2113 4961 5457 2113 Size: 2848 496 2414 Fragments arranged by size: 2848 2414 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3208 3969 4997 1540 Size: 275 186 12 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 3819 3904 4975 3819 Size: 85 1071 4602 Fragments arranged by size: 4602 1071 85 BsiWI C'GTAC_G Cuts at: 2482 2482 Size: 5758 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 3077 3819 3904 4975 1391 Size: 399 427 860 742 85 1071 2174 Fragments arranged by size: 2174 1071 860 742 427 399 85 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 2512 3647 5377 2512 Size: 1135 1730 2893 Fragments arranged by size: 2893 1730 1135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2009 3113 844 Size: 1165 1104 3489 Fragments arranged by size: 3489 1165 1104 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2841 4253 4427 65 Size: 1183 1593 1412 174 1396 Fragments arranged by size: 1593 1412 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 2096 2130 4402 4589 1700 Size: 110 262 24 34 2272 187 2869 Fragments arranged by size: 2869 2272 262 187 110 34 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 2840 794 Size: 2046 3712 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 2250 1184 Size: 1066 4692 BstYI r'GATC_y Cuts at: 1972 2629 3088 3455 3840 3857 5309 5395 5407 Size: 657 459 367 385 17 1452 86 12 Cuts at: 5407 5753 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 657 459 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2829 513 Size: 552 939 825 3442 Fragments arranged by size: 3442 939 825 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 3274 4068 4088 3274 Size: 794 20 4944 Fragments arranged by size: 4944 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2725 2760 529 Size: 35 2161 35 3527 Fragments arranged by size: 3527 2161 35 35 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DraIII CAC_nnn'GTG Cuts at: 2574 2574 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2733 3205 Size: 54 1425 50 275 18 180 723 472 Cuts at: 3205 4094 5723 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 723 472 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 3205 1537 Size: 275 198 1195 4090 Fragments arranged by size: 4090 1195 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2349 2687 3688 1353 Size: 996 338 1001 3423 Fragments arranged by size: 3423 1001 996 338 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4345 4719 4865 387 Size: 1064 138 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3821 4418 5205 5473 1239 Size: 393 2189 597 787 268 1524 Fragments arranged by size: 2189 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2657 2657 Size: 5758 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 EcoRV GAT'ATC Cuts at: 2262 2262 Size: 5758 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 2073 2090 4905 1678 Size: 395 17 2815 2531 Fragments arranged by size: 2815 2531 395 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2367 2399 4399 4431 4473 Size: 32 135 32 795 32 2000 32 42 Cuts at: 4473 4505 1373 Size: 32 2626 Fragments arranged by size: 2626 2000 795 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KasI G'GCGC_C Cuts at: 2069 2069 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 2973 3357 2973 Size: 384 5374 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3061 4872 5056 1305 Size: 1756 1811 184 2007 Fragments arranged by size: 2007 1811 1756 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2735 10 Size: 54 1425 343 903 3033 Fragments arranged by size: 3033 1425 903 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2517 2705 2834 3716 4075 4234 Size: 648 1044 307 188 129 882 359 159 Cuts at: 4234 518 Size: 2042 Fragments arranged by size: 2042 1044 882 648 359 307 188 159 129 MspA1I CmG'CkG Cuts at: 1606 2644 3174 3851 4999 5244 1606 Size: 1038 530 677 1148 245 2120 Fragments arranged by size: 2120 1148 1038 677 530 245 NaeI GCC'GGC Cuts at: 2074 2098 2074 Size: 24 5734 NarI GG'CG_CC Cuts at: 2070 2070 Size: 5758 NcoI C'CATG_G Cuts at: 513 1065 2004 2829 513 Size: 552 939 825 3442 Fragments arranged by size: 3442 939 825 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NgoMIV G'CCGG_C Cuts at: 2072 2096 2072 Size: 24 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 2010 3205 2010 Size: 1195 4563 NsiI A_TGCA'T Cuts at: 2845 2845 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 3116 4661 1132 Size: 97 1887 1545 2229 Fragments arranged by size: 2229 1887 1545 97 PasI CC'CwG_GG Cuts at: 1348 2108 1348 Size: 760 4998 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2549 1613 Size: 936 4822 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PstI C_TGCA'G Cuts at: 830 3110 830 Size: 2280 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 2644 2644 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SexAI A'CCwGG_T Cuts at: 2765 2765 Size: 5758 SfcI C'TryA_G Cuts at: 826 1045 3106 4241 4922 5113 826 Size: 219 2061 1135 681 191 1471 Fragments arranged by size: 2061 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2071 2071 Size: 5758 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 2072 1700 Size: 372 5386 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2306 2506 2963 2996 3862 4763 Size: 197 926 363 200 457 33 866 901 Cuts at: 4763 5025 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 866 457 363 262 200 197 33 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 2777 3682 5 Size: 47 2725 905 2081 Fragments arranged by size: 2725 2081 905 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5758 StyI C'CwwG_G Cuts at: 513 1065 2004 2829 3197 513 Size: 552 939 825 368 3074 Fragments arranged by size: 3074 939 825 552 368 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3785 3802 3955 4140 4585 325 Size: 1255 2205 17 153 185 445 1498 Fragments arranged by size: 2205 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3022 4004 5716 Size: 274 80 33 51 1443 1045 982 1712 Cuts at: 5716 96 Size: 138 Fragments arranged by size: 1712 1443 1045 982 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5718 5750 5718 Size: 32 5726 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI