################################## # NO CUT ENZYMES ( 206 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BciVI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AciI 282 GCGG CCGC(-3/-1) AlwI 58 GGATC GGATC(4/5) AlwNI 301 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG BbvCI 23 CCTCAGC CCTCAGC(-5/-2) BcgI 222 GCAAGTTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 216 TGATCA T^GATCA BfaI 244 CTAG C^TAG BsaJI 91 CCACGG C^C[ACGT][ACGT]GG BseRI 21 CTCCTC GAGGAG(10/8) BsmAI 73 GTCTC GTCTC(1/5) BsrI 55 ACTGG ACTGG(1/-1) BstYI 58 GGATCT [GA]^GATC[CT] DsaI 91 CCACGG C^C[GA][CT]GG FbaI 216 TGATCA T^GATCA MaeI 244 CTAG C^TAG MaeIII 52 GTCAC ^GT[ACGT]AC MflI 58 GGATCT [GA]^GATC[CT] NlaIII 214 CATG CATG^ SapI 259 GAAGAGC GCTCTTC(1/4) SfaNI 190 GCATC GCATC(5/9) TaqI 296 TCGA T^CGA Tsp45I 52 GTCAC ^GT[GC]AC TthHB8I 296 TCGA T^CGA XhoII 58 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BpmI 125 CTGGAG CTGGAG(16/14) 265 CTGGAG BsiYI 86 CCCCACCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 319 CCCTCTTCAGG BslI 86 CCCCACCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 319 CCCTCTTCAGG DpnII 59 GATC ^GATC 217 GATC EarI 321 CTCTTC CTCTTC(1/4) 259 GAAGAG Ksp632I 321 CTCTTC CTCTTC(1/4) 259 GAAGAG MboI 59 GATC ^GATC 217 GATC MseI 200 TTAA T^TAA 287 TTAA MspA1I 26 CAGCTG C[AC]G^C[GT]G 301 CAGCTG MwoI 282 GCGGTTTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 291 GCCCATCGAGC NdeII 59 GATC ^GATC 217 GATC PvuII 26 CAGCTG CAG^CTG 301 CAGCTG Sau3AI 59 GATC ^GATC 217 GATC Tru9I 200 TTAA T^TAA 287 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BbvI BstF5I DdeI Fnu4HI FokI HinfI HphI ItaI MboII MnlI TfiI TseI TspRI